Objective To analyze the occurrence of sharp injuries among health care workers(HCWs),and put forward suggestions for safety management of sharp inj uries.Methods Recorded form of sharp inj uries reported in a hospital in August 2006-July 2015 were analyzed retrospectively,relevant data were analyzed statistically.Results A total of 211 sharp injuries occurred among HCWs were reported,sharp injuries mainly occurred among nurses(n=118,55.92%)and doctors(n=83,39.34%);117(55.45%)sharp injuries occurred in HCWS whose length of service was≤5 years;the main occurrence locations of sharp injuries were surgical ward(n=67,31.75%),internal medicine ward(n=65,30.81%),and outpatient as well as emergency departments(n=33,15.64%);the main de-vices causing sharp injuries were syringe needle(n=91,43.13%),suture needle(n=34,16.11%),and scalp nee-dle/transfusion needle(n=27,12.80%);the main injury-related manipulations were all types of injection(n=40, 18.95%),intravenous transfusion (n=38,18.00%),and surgical suturing/cutting (n=36,17.06%).The main opportunities of injuries was during manipulation(treatment,examination,operation,n=109,51.66%).Among bloodborne pathogens contaminated devices,contaminated by HBV,HCV,and HIV were 95 (76.61%),25 (20.16%),and 4 cases(3.23%)respectively.There was no bloodborne pathogen infection occurred.155(73.46%) of 211 HCWs were inoculated hepatitis B vaccine.Conclusion Prevention of sharp injuries should be focused on nurses and doctors who work for≤5 years,HCWs in surgical ward,internal medicine ward,and outpatient as well as emergency departments.Prevention of sharp inj uries during manipulations such as inj ection,intravenous transfu-sion,suturing/cutting should be intensified.Related hospital departments should improve the reporting process and intensify follow-up.

Objective To study the mechanism of reinforcing kidney and activating blood therapy for the apoptosis of endometriosis.Methods Female SD rats aged 8~12weeks were grouped as model group,western medicine group,Chinese medicine group and integrated Chinese and western medicine group,with 15 in each group.The light microscope,electron microscope,TUNEL method,and immunohistochemical method were adopted to observe the pathomorphology,positive rate of apoptotic cells and the positive expression of apoptosis-regulating gene bcl-2 and bax.Results The apoptosis of endometriosis tissues in the integrated Chinese and western medicine group was the most obvious result with the highest apoptosis index,and the positive expression of apoptosis regulating gene bcl-2 was downward regulated and that of apoptosis regulating gene bax upward regulated,so bcl-2/bax decreased.Then it was Chinese medicine group,western medicine group,and model group.Conclusion The occurrence of endometriosis is related with the lowering of ability of apoptotic cells.Chinese medicines with the functions to reinforce kidney and activate blood can promote the ability of apoptotic cells in endometriosis to quicken the atrophy and regression of endometriosis tissues.

Barium ; urine ; Spectrophotometry, Atomic ; methods

Objective To study the chronic neurotoxicity of aluminum through observing the adverse effect of long term intake of aluminum on the memory of rats.Methods 40 Wistar rats were randomly divided into 4 groups and given Al2(SO4)3 through drinking water for 6 months,the concentration of Al3+ in the waters were 0.12 mg/L,40 mg/L,400 mg/L and 2 000 mg/L respectively,then passive avoidance tests were carried out.One month before pregnancy,the dams were given Al3+ of 40 mg/L and 2 000 mg/L respectively,after the offspring were born,the group given high[Al3+] was subdivided into two groups,one continuously drank the water with high [Al3+],the other drank the water without Al3+.The dams drinking water with low [Al3+] and their offspring continued to drink the same water as before.At the age of three months,all the offspring were examined with passive avoidance tests.Results The memory acquirement in all adult rats were not abnormal,but memory retention were severely damaged (P0.05).The rats continuously drinking water with high [Al3+] after birth had significant damage both in memory acquirement and in retention (P

Objective To study on the relationship of PTEN with clinicopathological parameters and prognosis of esophageal squamous cell carcinoma patients.Methods The expression of PTEN was detected in 62 cases of esophageal squamous cell carcinomas by immunohistochemistry.Results(1)PTEN expres- sion is negatively correlated with the depth of invasion,lymph node metastasis,distant metastasis,pTNM stage and degree of differentiation.(2)The difference of survival is significant between high and low expres- sion groups.Conclusion PTEN is correlated negatively with the clinicopathological parameters reflecting the malignant biological behavior,and is one of the significant prognostic predictors by univariate analysis.

OBJECTIVETo investigate the intervention effect and mechanism of compound Ginkgo biloba (CGB) preparations on nonalcoholic fatty liver disease (NAFLD).

METHODThe C57BL/6 mouse NAFLD model was induced with high fat diets. Since the 2nd week after modeling, the mice were orally administered with 600 and 200 mg x kg(-1) x d(-1) CGB for eight weeks. The levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TG), cholesterol (CHOL) and LPS in serum, as well as pathological changes and expression of tumor necrosis factor-alpha (TNF-alpha) in hepatic tissues were observed. Changes in intestinal tight junction proteins ZO-1, Occludin, Claudin-1 in intestinal tissues were determined under microscopy.

RESULTCompared with the normal group, the model group showed obvious fatty degeneration in rat livers, with notable increase in TNF-alpha expression (P < 0.01), significant increases in ALT, AST, TG, CHOL and LPS in serum (P < 0.01, P < 0.05), injury in intestinal tight junction proteins, and remarkable declines in ZO-1, Occludin and Claudin-1 (P < 0.01). Compared with the model group, CGB high and low dose groups showed obvious relieves in fatty degeneration in rat livers and injury in intestinal tight junction proteins, significant reductions in TNF-alpha expression (P < 0.01, P < 0.05) and AST, TG, CHOL and LPS in serum (P < 0.01, P < 0.05) and remarkable increases in ZO-1 and Occludin expressions (P < 0.05).

CONCLUSIONCGB can protect intestinal tight junction proteins, reduce intestinal leakage, relieve fatty degeneration and inflammations in livers and prevent NAFLD occurrence and development.

Alanine Transaminase ; genetics ; metabolism ; Animals ; Aspartate Aminotransferases ; genetics ; metabolism ; Cholesterol ; metabolism ; Drugs, Chinese Herbal ; administration & dosage ; Fatty Liver ; drug therapy ; enzymology ; genetics ; metabolism ; Ginkgo biloba ; chemistry ; Humans ; Male ; Mice ; Mice, Inbred C57BL ; Triglycerides ; metabolism

Objective To explore the inhibitory effects of Zibu Piyin Recipe(ZBPYR)serum on neuron apoptosis induced by tunieamyein(Tm,5 μg/ml)and its mechamsm in vitro by using sero-pharmacological method.Method Totally 12 healthy adult male SD rats(220～250 g)(SPF)were divided randomly into control group and ZBPYR group,6 in each group,then the blank and ZBPYR serum were prepared.The mouse.neuroblastoma cell line Neum2a cells were treated with Tunicamycin(Tin,an inhibitor of N-glycoslytion)to establish the endoplasmic reticulum(ER)stress model.The cells treated by ZBPYR aerum of different concentrations were interventional groups,and the cells treated by blank serum were control group.The viability of Neuro2a cells was meusurcdd by MTT assay.Flow cytometry wus applied to observe the apoptosis of Neuro2a cells.Western blotting was utilized to detect the protein expressions of two molecules,ER molecular chaperone-ucose regulated protein 78(CRP78)and transcriptional factor CCAAT/enhancer-binding protein-homologous protein(CHOP).The results were analyzed by sNK-q test.Results Compared to Tm group(cell viability 0.1673±0.0213,apoptotic rate 62.7050±1.4056),The cell viability of interventional groups(5%0.5295±0.0373,10%0.5843±0.0428,15%0.6274±0.0324)increased significantly(P<0.05);and the apoptotic rate(5%47.8733±2.8166,10%46.3366±1.2748,15%39.8833±1.0524)reduced significantly(P<0.05).The protein expressions of GRP 78(5%2.1228±0.2251,10%1.3293±0.9443,15%;15%0.0931±0.1168)and CHOP(5%1.1776±0.2927,10%0.7290±0.1708,15%0.6577±0.1883)of interventional groups reduced significantly compared with Tm group(GRP78 2.9149±0.5355;CHOP 1.6611±0.2913)P<0.05.Condusions ZBPYR serurn could increase the cell viability of Neuro2a cells treated with Tm and inhibit cell apoptosis.Thereby it may have neuroprotective effects,and the mechanism may be associated with the inhibition of ER stress and apoptosis pathway.

"Vessels Collateral Theory", as the inherit and development of "blood vessels" in Huangdi Neijing, is a systematic system by integrating severe vascular diseases. In this article, by taking cardiovascular diseases (CVD) as a cut-in point, roles of "minute collateral-microvascular" lesions in the occurrence and development of CVD were further explored. The interventional effect of collateral-unblocking medicines under the guidance of Vessels Collateral Theory was also in-depth explored, hoping to leading Chinese medical prevention and treatment of CVD.
Cardiovascular Diseases ; drug therapy ; Humans ; Medicine, Chinese Traditional ; Research

Objective To explore the expression of caspase-3 in skeletal muscle of the mice in the state of cancer, and to elucidate the relationship between Caspase-3 and apoptosis,consumption of skeletal muscle protein in cancer cachexia.Methods 48 male BALB/c mice were randomly divided into cancer cachexia group and control group (n=24).The mice in cancer cachexia group were inoculated with mouse colon 26 adenocarcinoma cells.The body weights of the mice in two groups were detected daily.Eight mice in each group were executed to test the weight of left gastrocnemius, fiber crosscut area, the expression levels of tumor necrosis factor-α(TNF-α), interleukin-6 (IL-6),Caspase-3 proteins and the apoptotic rate of gastrocnemius cells on day 8,14,and 20,respectively. Results The mice in cancer cachexia group appeared cachectic symptoms on day 14,the non-tumor body weight was decreased more than 20% of that in control group (P<0.05).Compared with control group at the same time, the mouse body weight,non-tumor body weight,the weight of left gastrocnemius and the fiber crosscut area of the mice in cancer cachexia group were obviously decreased with the prolongation of inoculation time (P<0.05 ), whereas the expression levels of TNF-α,IL-6,Caspase-3 proteins and the apoptotic rate of muscle cells were obviously increased after tumor inoculation (P<0.05).The level of Caspase-3 protein was negatively correlated with the weight of gastrocnemius and fiber crosscut area (r=-0.716,P<0.05;r=-0.694,P<0.05),and the level of Caspase-3 was positively correlated with the levels of TNF-αand IL-6 (r=0.742,P<0.05;r=0.675,P<0.05).Conclusion Caspase-3 may be a key factor in the protein comsumption of skeletal muscle in cancer cachexia.

Objective Matrix metalloproteinase-10 (MMP-10) has been shown to be highly associated with atherosclerosis.Recent studies showed that levels of MMP-10 were elevated in infarcted tissues in acute ischemic stoke.However,serum levels of MMP-10 in patients with acute ischemic stroke have never been studied previously.This study aims to investigate the serum levels of MMP-10 in patients with acute ischemic stroke,and evaluate the association of serum levels of MMP-10 with stroke subtypes based on Trial of Org 10 172 in acute stroke treatment classifications,the severity of stroke,risk factors and carotid artery plaque.Methods The circulating levels of MMP-10 were measured by enzyme linked immunosorbent assay in 194 subjects,including 109 patients who were diagnosed as acute ischemic stroke in the Department of Neurology,Shengjing Hospital,China Medical University from April to December 2012,and the 85 healthy controls.Results Patients with acute ischemic stroke had higher serum levels of MMP-10 compared with healthy controls (6.59 (6.07,7.31) μg/L vs 5.16 (3.87,5.94) μg/L,Z =8.33,P ＜ 0.01).NIHSS score had positive correlation with serum levels of MMP-10 (r =0.204,P =0.037).Classified by risk factors,we compared the MMP-10 levels of subsets,and results displayed that statistically significant difference existed between dyslipidemia subset and non-dyslipidemia subset (Z =2.07,P =0.042).MMP-10 levels had positive correlation with serum levels of LDL-cholesterol (r =0.248,P =0.040),but negative correlation with thrombin-activatable fibrinolysis inhibitor (TAFI;r =-0.208,P =0.030).The subset with unstable plaques had higher MMP-10 levels than that with stable plaque (6.62 (6.13,7.36) μg/L) vs 6.10 (6.00,6.46) μg/L,Z =2.12,P =0.034),implying the relationship of MMP-10 and atherosclerosis.Conclusions Patients with acute ischemic stroke have higher serum levels of MMP-10 compared with the healthy controls,and MMP-10 levels have positive correlation with the severity of stroke.MMP-10 is associated with the subtypes of stroke classified by risk factors,and dyslipidemia subset has higher levels of MMP-10 than that of non-dyslipidemia subset.MMP-10 has positive correlation with LDL-cholesterol,but negative correlation with TAFI.MMP-10 may be involved in the process of formation and disruption of unstable plaques,which contribute to the stenosis of arteries and onset of acute ischemic stroke.