Objective To evaluate the academic level and the popularity of Chinese Journal of Orthopaedics from the point view of citation. Methods According to the information of Chinese Medical Citation Index (CMCI), the amount and distribution of the original articles in Chinese Journal of Orthopaedics cited by the journals included by CMCI were statistically analyzed. The data analysis included the percentage of cited articles, the number of citation of a single article, authors of most cited articles, geological regions of cited articles, citing journals, the self citing rate and years of citation. Results The percentage of cited papers (663 articles, 3728 times) in all published 1 182 articles in Chinese Journal of Orthopaedics from 2005 to 2009 was an average of 56.09%. The average time of original articles cited by other researchers was 5.62. The highest one was citated by 104 times. 210 articles (31.68% of total papers) were cited 5 or more times, and the all cited times from them were 2835 (76.04% of total cited times). 663 cited articles were written by 485 authors from different countries. The most frequently cited author wrote 12 papers. The number of authors with one paper cited was 386 (79.58% of total authors). The cited authors are from the mainland, HongKong, and Taiwan in China, as well as USA, Canada, France. Beijing, Shanghai and Tianjin are leading cities in orthopaedic research. Beijing was the first place (32.16% of total authors). There were 445 citing journals. There was 217 items self cited; the self- citing rate is 0.058. Conclusion Chinese Journal of Orthopaedics has provided high quality articles and has a strong influence in the field of medical research. It has been become an important resource for orthopaedic researchers and a vital medical journal in China.

Objective To investigate the effect of old myocardial infarction on the heart rate variability.Methods Totally 86 patients with old myocardial infarction and 78 healthy people were enrolled in the study.Twenty-four-hour heart rate variability in the time domains including NN,SDNN,SDANN,RMSSD,and PNN50 and the frequency domains including VLF,LF,HF,and LF/HF were analyzed.Results Both the time domain and frequency domain indexes of the heart rate variability in patients with old myocardial infarction significantly decreased than those in the healthy peolple（P 0.05）.Conclusion There might be autonomic nervous system dysfunction in the patients with old myocardial infarction.

DNA/genetics* ; Genotype ; Humans ; Infant ; Polymorphism, Single Nucleotide

Animals ; Atherosclerosis ; prevention & control ; Biflavonoids ; pharmacology ; Catechin ; pharmacology ; Grape Seed Extract ; pharmacology ; Humans ; Proanthocyanidins ; pharmacology

Objective To observe the therapeutic effects of oxygen inhalation combined with relaxation training on patients with generalized anxiety disorder (GAD).Methods A total of 188 GAD patients were divided into the study group and the control group randomly.The two groups were treated with conventional antianxiety drugs (paroxetine),in addition to conventional medical therapy.The study group was aided with oxygen inhalation and relaxation training,the control group was given benzodiazepine.The scores of Hamilton anxiety rating scale (HAMA),global improvement(GI) [ a part of clinical global impression (CGI) ],and treatment emergent symptom scale (TESS) were used to evaluate clinical effects and adverse reactions before and after 4-week treatment.The indexes of breathing,heart rate and blood pressure were evaluated before and after treatment.Results The scores of HAMA scale and GI in the two groups were significantly different after treatment( P ＜ 0.05 ),and the study group changed significantly( P ＜ 0.05 or P ＜ 0.01 ). The adverse reactions of the two groups were slight,there was no significant difference of TESS before and after treatment ( P ＞ 0.05 ). There was no significant difference in terms of breathing,heart rate and blood pressure between the two groups before treatment( P ＞ 0.05 ),but were all lower after treatment the study group changed more apparently and significantly(P ＜ 0.01 ).Conclusions Based on conventional drugs treatment oxygen inhalation combined with relaxation training could improve anxiety and somatization rapidly,there was no obvious adverse reaction and the patients compliance was good,so the treatment was worth to use in clinic.

Hepatitis C virus(HCV)is the major etiology of non-A,non-B hepatitis.At present,neither a vaccine nor any effective therapy is available.Multi-epitope DNA vaccine(minigenes/epigenes)is a novel nucleic acid vaccine which can induces high effective cellular and humoral immune responses and has good perspective in clearing Hepatitis C virus through screening and assembling optimal antigen epitope genes(T cell、B cell epitope included).It is advanced in covering more HCV subtypes,inducing comprehensive anti-HCV immune responses and reducing the negative influence caused by irrelevant,disturbing and suppressive sequence as far as possible through selecting the most potential protective epitopes in DNA vaccine design.The recent research progress on HCV compound multi-epitope DNA vaccine and future prospects were reviewed.

Objective To investigate effect of interferon gemma on immune factor and CD69, CD107a in patients with delayed type hypersensitivity by interferon gamma.Methods 76 cases with delayed type hypersensitivity were selected and divided into 2 groups.38 cases in control group were treated conventional therapy, 38 cases in experiment group were treated by interferon gamma.Peripheral blood CD69, CD107a, immune factor, T cell subsets and the treatment efficiency were compared after the treatment.Results Compared with the control group, the serum CD69 and CD107a levels were lower in the experimental group (P<0.05), serum IgG and IgA levels were higher, the serum IgE level was lower (P<0.05), and the CD3 +,CD4 +,CD4 +/CD8 +level was higher, serum CD8 + was lower (P<0.05) .The effective rate of the treatment group was significantly higher than that of the control group (P<0.05) .Conclusion Interferon gamma has good clinical effect in the patients with delayed type hypersensitivity, and can effectively reduce the levels of CD69 and CD107a in the peripheral blood, and regulate the immune function of the body.

Objective To discuss the values of renal function indicators in predicting chronic kidney disease (CKD) .Methods According to eGFR ,212 cases of CKD patients were divided into three groups :group A (eGFR> 60 mL · min-1 · 1 .73 m -2 ) , group B (eGFR 30-60 mL · min-1 · 1 .73 m-2 ) ,and group C (eGFR<30 mL · min-1 · 1 .73 m-2 ) .175 cases of patients with the risk of CKD and 60 cases of healthy people were selected at the same time and brought into high‐risk group and normal control group ,respectively .The ratios of microalbumin and creatinine in urine (UmAlb/Cr) ,and the serum levels of Urea ,creatinine(Cr) , uric acid(UA) ,cystatin C(CysC) ,β2‐microglobulin(BMG) and homocysteine(Hcy) in all the groups were detected .Results There were differences in the levels of CysC and BMG among the three groups of CKD patients(P<0 .05) ,as well as between high‐risk group and normal control group ,with significant difference (P<0 .05) .Conclusion CysC and BMG can be used as effective indica‐tors of CKD prediction .

It is important to explore the fundamental medical concepts which can enhance the overall quality of medical students in the department of geriatrics during clinical practice. These funda-mental medical concepts include medical humanistic spirit,holistic medicine,health management and patient-physician communication. Specific measures include improving emotional communication of clini-cal interns in department of geriatrics with elderly patients,training student's thinking as a whole towards multiple system disease in the elderly,training students to assess and manage elderly patients from a vari-ety of perspectives and improving doctor-patient communication skills by routine clinical teaching and teaching ward round. All these concepts will help improve the working ability and medical ethics of clini-cal interns and enable them to adapt to the clinical work quickly. So they can become qualified doctors.

Objective:To observe expression of stathmin gene in laryngeal squamous cell carcinoma(LSCC) and relation between expression of stathmin gene and occurance and development of LSCC.Method:The expression of the stathmin gene was determined in 35 LSCC of specimens and 18 normal laryngeal tissues(NLT) of specimens by in situ hybridizaion with Digoxigenin labled probe of stathmin mRNA.Result:Expression of stathmin gene was observed in 35 cases of laryneal squamous cell carcinoma tissue (positive rate, 69%) and positive signal was observed in both cytoplasm and nuclear. Among 18 cases of normal tissue, only 6 showed weak positive signal. There was significant difference in expression of stathmin gene between laryneal squamous cell carcinoma tissue and nomal tissue.Conclusion:Expreesion of stathmin gene may play a key role in the pathogenisis and development of laryneal squamous cell carcinoma. It may be a very important biotherapy target in the treatment of laryneal squamous cell carcinoma.