The MLN64 gene,which is localized in q12-q21 of the human chromosome 17,encodes a novel transmembrane protein containing 445 amino acids .The C-terminus of MLN64 shares significant homology with the steroidogenic acute regulatory (StAR)protein,while its N-terminal domain exhibits a function of targe-ting the protein to late endosomes .MLN64 is likely to be involved in cholesterol transport and synthesis of steroid hormones.MLN64 gene,coamplified with C -erbB-2,is overexpressed in certain breast carcinomas and exerts an influence on biological characteristics of breast cancer cells .The high levels of MLN 64 observed in some breast carcinomas could contribute to the growth and progression of these tumors through increased intratumoral steroido -genesis,and is considered as a predictive factor of breast cancer prognosis .

Objective To investigate the different gender and untreated hyperthyroidism parameter difference (hyperthyroidism) and hemoglobin of red blood cells in peripheral blood of patients with relatively healthy people.Methods Collecting blood samples of patients with hyperthyroidism,with EDTA-K2 anticoagulant,using Sysmex XE-2100 automated hematology analyzer analysis of peripheral blood hemoglobin in red blood cell parameters and using healthy people as healthy control.Results Healthy control group,male,female red blood cells and hemoglobin in patients with hyperthyroidism were increased,the difference was statistical-ly significant (P <0.05).Conclusion Newly diagnosed hyperthyroidism value and erythrocyte hemoglobin values higher than nor-mal.

OBJECTIVE: Autologous bone transplantation has been extensively used clinically, but the scant bone source eligible for transplantation cannot meet the demand in large bone defect repair. Tissue engineering seems to provide a way out, by inducing bone regeneration for repairing bone defect. In this article, the authors review the development in bone tissue engineering achieved in recent years.DATA SOURCES: A literature review was conducted with Google and Medline-based search for reports published in Chinese on bone tissue engineering in the last 3 years, as well as for worldwide literature published from January 1964 to December 2002, using the key words for retrieval of bone tissue engineering, scaffold, hydroxyapatite composites.STUDY SELECTION: Fifty-six articles were selected, which documented in vtiro bone culture, non-crystal medical biomaterials, development of bone tissue engineering, experimental and clinical trials of bone tissue engineering and clinical application of the results of bone tissue engineering.DATA EXTRACTION:From the 56 articles, 14 were excluded for repetition of contents. The remaining 42 were classified for reviewing and 21 of these were enrolled as references here.DATA SYSTHESIS: Autologous bone grafting is the usual choice for bone defect repair. The grafts include allografts and xenograft of spongy bone and compact bone, joints, decalcified bone matrix, marrow, ceramics and ceramics composites. But the effect of repair or reconstruction of large bone defect as left by excision of large bone tumors is not satisfactory. Tissue-engineering studies on composites for repair of the damaged nerve, tendon and skin are still in their infancy, while studies of bone scaffold materials has made great progress.CONCLUSION: Great breakthroughs have been made in tissue engineering and relevant studies of tissue defect and function impairment is fast developing. As the structure of motor system and skeletal system are relatively simple,and the bone or cartilage defects clinically common, bone tissue engineering might first enter clinical use in the field of orthopedics.

Cancer stem cells(CSCs) are a group of tumor cells with stem cell-like properties such as infinite self-renewal and multi-differentiation potential. Accumulating evidence strongly indicates the presence of cancer stem cells in a variety of solid tumors. Although rare, CSCs play important roles in tumor initiation, progression, recurrence and metastasis. Research of gastric cancer stem cells is still in its infancy. Specific surface markers of gastric CSC are yet to be identified. Active researches are currently underway to investigate the existence and source of CSC in gastric cancer.

S2001 field X-ray vehicle is used to perform X-ray examination in the field hospital, while the disadvantages of the traditional film-screen radiography restrict its role of field examination in this digital era. By analyzing the detail of modern digital X-ray radiography, and comparing three different digital X-ray flat detectors, a solution is put forward to update S2001 field X-ray vehicle to a field digital X- ray radiography vehicle by using Canon CXDI digital X- ray detector.

Thyroid cancer is more common in thyroid diseases, because of its slow growth and good prognosis, different scholars have different views on lymph node dissection. During the study of definition and positioning methods of sentinel lymph node biopsy, discuss the necessity of cervical node dissection and the application of cleaning scope in thyroid cancer surgery.
Humans ; Lymph Nodes ; pathology ; Sentinel Lymph Node Biopsy ; Thyroid Neoplasms ; diagnosis ; pathology

Objective To investigate the effects of DNaseⅠ-treated streptococcal antigens on peripheral blood mononuclear cells(PBMC)proliferation in psoriasis patients.Methods PBMC from 15 patients with psoriasis and 10 healthy controls were stimulated with three concentrations(2,10,25?g/mL) of streptococcal antigens(SA), nd DNaseⅠ-treated streptococcal antigens(DNaseⅠ-SA).After 72 h of culture,cellular proliferation was determined by ~3H thymidine incorporation.Results The DNaseⅠ-SA,at all the concentrations tested,was less potent than the corresponding concentrations of SA(P0.05),however,the lower concentrations(2,10?g/mL) of DNaseⅠ-SA were less potent than those of SA(P0.05).Also,the extent of proliferation response to SA and DNaseⅠ-SA was higher in the patients than in the controls(P

Atomic force microscope(AFM) has one important feature that it is used to scan the samples with non-modified and modified probes to obtain sample appearance,and force-distance curve at certain point,based on which the adhesion,bond force and mechanical properties of the sample can be obtained.Until recently,the application of the AFM to measure the mechanical properties of biological sample is very extensive,which is significant in biomedicine and clinical medicine.This paper introduced the force curve theory of AFM,and reviewed the application of AFM to measure the mechanical properties of biological sample including elasticity,adhesion,stiffness,the interaction between antibody and antigen of the cell.In addition,we prospected the application and development of AFM to analyze cell mechanical properties.

Objective To observe the effect of clapping acupoint on hyperosteogeny of lumbar vertebra. Methods Ninety-eight cases were randomly divided into 65 cases treated by clapping Ashi point in treatment group and 33cases treated by simple acupuncture in control group.Results Among 65 cases in treatment group, 52 cases were cured and 13 cases were not cured; of 33 cases in control group, 19 cases were cured and 14 cases were not cured, and there had significant difference in cure rate between the two groups, x2 =5.51, P ＜0.05. Conclusion Treatment of hyperosteogeny by clapping acupoint was better than simple acupuncture therapy.

Objective To investigate the prevalence of cardiovascular and cerebrovascular diseases in the army old male hypertensive patients in Beijing. Methods From October 2011 to April 2014, old male patients in the geriatric department of the General Hospital of Chi-nese People's Liberation Army were enrolled and categorized into 3 groups: normotensive, well-controlled hypertensive and poorly-con-trolled hypertensive groups. The prevalence of cardiovascular and cerebrovascular diseases was recorded and binary logistic regression was used to analyze the differences among the groups. Results 719 old male patients were enrolled, 228 in normotensive group, 389 in well-con-trolled hypertensive group and 102 in poorly-controlled hypertensive group. Compared to normotensive group, there was more incidence of angina (OR=2.547, P<0.001), myocardial infarction (OR=2.493, P=0.003), cerebral thrombosis (OR=3.438, P=0.003) and peripheral artery atherosclerotic disease (OR=1.766, P=0.026) in the well-controlled hypertensive group, and it was more of angina (OR=4.123, P<0.001), la-cunar infarction (OR=2.810, P=0.020), cerebral thrombosis (OR=3.484, P=0.001) and peripheral artery atherosclerotic disease (OR=3.773, P<0.001) in the poorly-controlled hypertensive group. There was not significant difference of prevalence of cerebral hemorrhage (0.9%, 1%and 1%, respectively). Conclusion In the army old male hypertensive patients in Beijing, control rate of blood pressure was higher than com-mon population, but the prevalence of ischemic diseases was still higher than in the normotensive patients. Anti-hypertension and control of other risk factors could reduce the mobidity of cerebral haemorrahge.