OBJECTIVE:To study compatible stability of pazufloxacin mesilate for injection with three kinds of hemostatics in sodium chloride injection.METHODS:The changes of the mixtures in appearance,pH values,insoluble particles and the content of pazufloxacin mesilate within 8 hours after mixing of pazufloxacin injection with etamsylate,Vitamin K1 or aminomethylbenzoic at 25 ℃ under natural illumination in a simulated clinical common concentration were followed.RESULTS:There were no significant change in the appearance,pH value and the content of pazufloxacin mesilate within 8 hours after mixing of pazufloxacin mesilate for injection with aminomethylbenzoic injection or etamsylate injection;when mixed with Vitamin K1,there were no significant change in the appearance,pH and the content of pazufloxacin mesilate within 2 hours,but the insoluble particles was found to be unqualified after 1 hour of mixing,and white precipitate appeared after 4 hours and the content of pazufloxacin mesilate decreased to some degree.CONCLUSION:The mixture of pazufloxacin mesilate for injection and etamsylate or aminomethylbenzoic was stable within 8 hours at room temperature.The mixing of pazufloxacin mesilate for injection with Vitamin K1 should be conducted with caution.

Child ; Epilepsies, Partial ; Humans

Mechanisms of UV-B-induced apoptotic regulation in yeast Saccharomyces cerevisiae were studied. The results showed that UV-B irradiation indeed inhibited the growth of yeast cells as well as induced extensive apoptosis during 96 h experiment period. However, survival of 96 h irradiated cells remained 10% while most control cells finally dead after re-growth under UV-B irradiation for 12 d. And by exposed to 0.01 mol/L or 0.1 mol/L H2O2 for 30 min, survival rate of 24 h irradiated cells were 3.0-fold or 3.2-fold than control, respectively. By to heat shock for 30 min or 60 min, survival rate of 24 h irradiated cells were 3.5-fold or 9.0-fold than control, respectively.

Cerebral Palsy ; diagnosis ; etiology ; physiopathology ; Hearing Disorders ; etiology ; Humans ; Interleukin-6 ; analysis ; Prognosis ; Research Report

OBJECTIVE To investigate the advantages of nicardipine induced hypotension in endoscopic sinus surgery.METHODS Eighty patients underwent endoscopic sinus surgery under general anesthesia were randomly assigned into two groups(n=40 each):Group A(Enflurane Group), Group B (Nicardipine Group).The effect of controlled hypotension was compared between the two groups.The changes of MAP, HR were recorded during induced hypotension, the time of induction, maintenance and recovery in induced hypotension were recorded.RESULTS 1.The induced hypotension induction time in group A was significant shorter than that in group B, BP recovery time in group A was obviously longer than that in group B (P

Artemisinin is a traditional anti-malarial drug. In recent years, many researches indicated that artemisinin and its derivatives have obvious anti-tumor effects. Its mechanisms of anti-tumor lies in cytotoxicity, direct destruction of tumor cells due to oxidative stress reaction, inducing apoptosis, inhibiting of tumor cell proliferation, restraining tumor angiogenesis and performing immunological regulation. Compared with the traditional anti-cancer drugs, artemisinin can selectively inhibit tumor cells and manifestating no cross-resistance with traditional chemotherapeutic agents. Besides it can reverse the phenomenon of multi-drug resistant of tumor cells. Therefore, artemisinin, as an new type of anti-cancer drug, will be applied to a wide range of clinical practice.

Objective: To introduce the Raman spectrum, the optical tweezers physical principle and its working characteristics, unify the detail medical research work, summarize specific application situation of the Raman tweezers technology in the different cells domain. Methods: Use the optical tweezers to fix the living cells, simultaneously carry out Raman spectrometry on the living cell or the cell organ by using the laser Raman technology. By applying this technology, the samples will be captured in the suspending liquid. In an approximate physiological state, the single living specimens, such as the cells, the cell organs or the biological macro-molecules, will be studied and the real-time track to the research object physiological biochemistry process will be carried on, then the Raman spectrometry will be implemented to the living cells. Results: From the single cell level, Raman tweezers technology analyzes the oxygen ability and the deformability of red blood cells of normal persons and the Mediterranean Sea anemia patients, and implements the appraisal of blood red cell and the blood platelet of different species. The Raman tweezers technology reveals the differences between the organizational structure of the cancer cells and that of normal cells in the molecular level, providing important information and data for the cancer diagnosis and the mechanism analysis. The Raman tweezers technology has implemented the torsion and knotting of DNA molecules, and realizes the control and differentiation of human being's chromosome. Conclusion: The Raman tweezers technology is the prompt and effective tool for the real-time research of cell physiology and biochemistry changes, hopefully in the molecular level. It will become one of the most advanced tools to carry out examination and diagnosis of different kind of living cell. Surely it has a very bright prospect.

Objective To study the expression of Ephrin‐B4 receptor (EphB4) in hepatocellular carcinoma (HCC) and its clinical significance ,and to analyze the effect of EphB4 on the proliferation of HCC cells .Methods The expression level of EphB4 in HCC tissues and matched paracancerous liver tissues of 60 cases of HCC patients was assessed by reverse transcriptase‐polymer‐ase chain reaction (RT‐PCR) and immunohistochemistry .The correlation between the expression of EphB4 in HCC tissues and clin‐ical pathologic parameters was analyzed by chi‐square test .Univariate survival analysis was carried out by Kaplan‐Meier Log‐rank test .The effect of EphB4 on the viability of HCC cells was furtherly analyzed by MTS .Results The results of RT‐PCR showed that the mRNA level of EphB4 in HCC tissues (1 .39 ± 0 .80) was significantly higher than in matched paracancerous liver tissues (0 .56 ± 0 .33) ,the difference was statistically significant (P< 0 .01) .Data from immunohistochemistry showed that the positive rates of EphB4 protein in HCC tissues and matched paracancerous liver tissues were 81 .7% and 23 .3% ,respectively .Moreover ,the expression of EphB4 in HCC tissues was relevant to AFP level ,tumor size and TNM stage(P<0 .05) .The three‐year survival rate of HCC patients with positive expression of EphB4 protein was 22 .5% ,and that of HCC patients with negative expression of EphB4 protein was 54 .5% ,the difference was statistically significant (P<0 .05) .Overexpression of EphB4 significantly enhanced the ability of hepatocellular carcinoma cell proliferation (P<0 .05) .Conclusion EphB4 expression was significantly up‐regulated in HCC ,which was associated with HCC progression and prognosis ,and EphB4 could promote the proliferation of HCC cells ,which could be used as a marker of HCC progression .

Early diagnosis of inflammation and properly interfere to improve the prognosis are very important.However,it is difficult to verify inflammation or differentiate inflammation from infection in clinic,especially in the condition of underlying inflammation.Currently,radiopharmaceuticals based on researches of pathophysiology progress of diseases are studied constantly.This review summarizes the potential inflammation imaging radiotracers and their mechanisms,potential clinical application value.

Objective To evaluate the effect of enteral nutrision (EN) containing dietary fiber complex (DFC) on intestinal mucosal barrier in rats with experimental colitis. Methods Ninety six SD rats were induced into ulcerative colitis by acetic acid enema.than were divided into 3 groups. The rats in group C received non-DFC nutrison, while the rats in group Tl or group T2 received soluble dietary fiber (SDF) and insoluble dietary fiber (IDF) of the EN with different proportion (1:2 or 1:3, respectively). At the first-, third-,fifth- and seventh-day after the EN, the concentration of D-lactate and diamine oxidase (DAO) activity in plasma were detected and intestinal samples were obtained to observe the changes of intestinal mucosa. Results At the third-,fifth- and seventh-day,the D-lactate concentration and the DAO activity in plasma significantly reduced in group Tl and T2 when compared with group C (P<0. 05) , but there was no statistical difference between group Tl and group T2. The scores of histological damages of colon in the group Tl and group T2 decreased in comparison with group C at all time points (P < 0. 05). However, the scores of histological damages of colon significantly decreased in group T2 when compared with group Tl at day 5 and day 7 (P <0. 05). Conclusion EN containing DFC may reduce the intestinal permeability of colitis in rats, which has a protective effect on intestinal mucosal barrier. While different proportions of the SDF or IDF ratio result in different effects on the mucosal protection. This protective effect will be enhanced by increasing the ratio of IDF in a certain scale.