Isolation of ?- aminobutyric acid in Hedysarum polybotrys Was accomplished by HPTLC and the content of the sail component in the herb and its preparation determined by dual wave length seanning at ?5 580nn and ?R700nm, The method was simple and accurate.

According to TCM theory of the wholistic concept and the principles of syndrome differentiation and treatment, the characteristics of the pathogenesis of aplastic anemia (AA) were summarized and corresponding treatment was put forward on the basis of clinical observation and the study of literature. In the pathogenesis of AA, the deficiency of spleen and kidney is the primary aspect, fire attacking blood vessels is the secondary aspect and there is change because of blood stasis. In treatment, the method of invigorating spleen and tonifying kidney is adopted in order to get at the root, and releasing fire to stop bleeding is adopted in order to bring about a temporary solution, and activating blood circulation to dissipate blood stasis is adopted in order to produce blood. The outline should be caught hold of that consumption is primary aspect and fire is secondary aspect and there is change because of blood stasis in order to obtain satisfactory curative effect.

Objective Health education provided by medical staffs can promote the patients 'abilities of self management . This article explored the teaching mode of health education course for nursing students of college to undergraduate level . Methods Reform took place in the course of health education for nursing students of Grade 2009 to 2012 who studied in the medical school of col-lege to undergraduate in Nanjing Genernal Hospital .The unidirectional teaching method was replaced by trinity teaching method which included cases , situational analogue training and clinical situation experience .In their clinical internship , the same questionnaire was used by designated personnel to survey the satisfaction degree of the patients , the clinical teachers and the nurses themselves .Analysis and comparison were conducted on the materials of satisfaction degree from 2009 to 2011 . Results Significant difference was found in the satisfaction degree of the patients , the clinical teachers and the nurses before and after the application of trinity teaching model (93.4%vs 56.9%, 91.6%vs 59.7%, 99.3% vs 78.1%). Conclusion The trinity teaching model is of high practical value , which is worth popularizing .

Objective To study the correlation of heart rate variability(HRV) and autonomic nervous function in patients with anxiety neurosis, and provide evidence for prevention and treatment of anxiety disorder. Methods 90 patients with generalized anxiety disorder(GAD) and panic disorder(PD) and 90 healthy persons were tested by psychological questionnaire,the anxiety and depression were assessed by HAMA and HAMD. The average heart rate and HRV index were monitored by 24-hour ambulatory ECG. Results The average heart rate of the anxiety group was higher than the control group, compared with the control group the difference was significant ( P < 0. 05 ); The HRV parameters of the anxiety group were lower than the control group, compared with the control group the difference was significant( P < 0. 05 ) ;The HRV parameters of the GAD group were lower than the control group, the HRV parameters of the PD group were lower than the GAD group, the difference was significant ( P < 0. 05 ). Conclusion The patients with anxiety neurosis had high sympathetic nerve activity function and autonomic dysfunction, the PD patients had higher sympathetic nerve activity function and autonomic dysfunction than the GAD patients.

OBJECTIVE To investigate Epstein-Barr virus (EBV) infection and clinical symptoms among hospitalized children by detection of EBV in their throat swabs. METHODS During from Oct 2004 to Apr 2006, EBV-DNA was detected from throat swabs of inpatients in pediatric ward by the technology of fluorescence quantitative polymerase chain reaction. RESULTS Totally 3429 cases were detected, 738 cases were positive. The positive rate was 21.5%. In different age groups, the rate of EBV infection was 18.8% for ≤3 years old, 26.5% for 4-7 years old, 29.0% for 8-11 years old, and 31.7% for 12-15 years old, respectively. The rate of EBV infection increased significantly with age increasing (P

Objective In order to optimize patient's medical treatment processes, and to improve the working efficiency of health care professionals and the quality of medical services. Methods The design and application of the electronic prescription module were introduced, and its advantages and existing problems were discussed. Results The electronic prescription module were applied generally. Orthodox patient's medical treatment processes were changed and the prescription management was normalized. Conclusion The electronic prescription module is one of the core components of the workstation system for the out-patient physicians. It optimizes the patient's medical treatment processes and improves the efficiency and the quality of medical services.

Objective: To observe the clinical therapeutic effect of Pyretic Arthralgia granules in treating rheumatoid arthritis of moist heat arthralgia spasm syndrome,so as to provide scientific evidence in clinical Medication.Methods: Adopted random and positive parallel contrast methods,60 cases of active stage rheumatoid arthritis(RA) patients whom were differentiated of symptoms and signs as moist heat arthralgia spasm syndrome were divided into groups.Experimental group took Pyretic Arthralgia granules 2.0 g every time and 3 times a day orally;control group took Loxonin orally,60 mg every time and 3 times a day.Both course of treatment in two sets were 4 weeks.Results: Experimental group and control group all could improve present symptoms,physical signs and physical-chemical index.There was not significance variance in two groups comparing the disease therapeutic effect with the therapeutic effect of syndrome of traditional Chinese medicine.Conclusion: Pyretic Arthralgia granules has the effects of detumescence,alleviating pain and improving Joint function,and has not obvious poison and side reaction.It is good for further generalizing and applying.

Objective To investigate the status of post traumatic growth and role reinforcement in elder patients with cerebral infarction accepting the treatment of rehabilitation, and to explore the effect of role reinforcement on post traumatic growth. Methods Totally 303 patients with cerebral infarction accepting the treatment of rehabilitation were investigated by the Post Traumatic Growth Rating Scale and the Role Reinforcement Scale. Results The post traumatic growth and role reinforcement scores of the patients were (61.50 ± 7.19), (63.51 ± 9.28) points. The scores of each dimension of post traumatic growth were significantly positively correlated with the role reinforcement (r =-0.794--0.123, P < 0.05 or 0.01). Stepwise regression analysis showed that the influence factors on the post traumatic growth of the patients were the cultural level, age and each dimension of role reinforcement (P < 0.05). Conclusions For the patients with cerebral infarction accepting the treatment of rehabilitation, the level of post traumatic growth is in a moderate level, and the role reinforcement is in a strong level, and role reinforcement is the influencing factor of post traumatic growth.

Objective To survey the point prevalence of healthcare-associated infection(HAI) in 13 tertiary hospitals in Dongguan,understand the occurrence of HAI in tertiary hospitals,so as to provide guidance for the prevention and control of HAI.Methods Through bedside investigation and medical record reviewing,HAI among inpatients in 13 tertiary hospitals in Dongguan at 0:00-24:00 of September 24,2014 were investigated.Results A total of 11 344 patients were investigated,379 patients developed 404 times of HAI,HAI rate was 3.34％,HAI case rate was 3.56％.Incidence of HAI in the intensive care unit was highest (12.82％).The main infection site was respiratory tract(n =173,42.48％).The usage rate of antimicrobial agents was 30.50％,most were for therapeutic use (including therapeutic+ prophylaxis) (n =2 545,73.55 ％)and single use (n =2 689,77.72％),pathogenic microorganism detection of specimens from patients receiving therapeutic antimicrobial use was 53.79％.A total of 299 strains of pathogenic orgamisms were detected from infected patients,the major were Escherichia coli (n =56,18.73 ％),Pseudomonas aeruginosa (n =54,18.06 ％),and Klebsiella pneumoniae (n =35,11.71 ％),92 strains of multidrug resistant organisms(MDROs) were isolated,accounting for 30.77％ of total isolated pathogens.Conclusion The basic characteristics of HAI in tertiary hospitals in this city were preliminarily investigated,identification of high risk departments,high risk population,and high risk links should be strengthened,HAI prevention and control measures should be implemented.