Acupuncture Therapy ; Adult ; Female ; Humans ; Trigeminal Neuralgia ; therapy

Objective To determine the frequency of the cholesteryl ester transfer protein (CETP)-TaqIB polymorphism and investigate its relationship with plasma lipid levels and coronary hert disease(CHD). Methods Two hundred and thirty-eight patients with CHD (CHD group) and 203 age-matched controls( control group) were selected, the CETp-TaqIB mutation was detected by restriction fragment length polymorphism of the CETP gene. Results In the total subjects, the frequency of B1 and B2 alleles were 59.4%(262/441 ) and 40.6%( 179/441 ) respectively. Compared with that in control group, the frequency of CETP genotype BIBI was higher in CHD group [39.9%(95/238) vs 29.6% ( 60/203 ), P<0.05], and the frequency of B1B2 was lower in CHD group [44.1%(105/238) vs 53.7%(109/203), P< 0.05]. Compared with that in the B2 homozygotes, high density lipoprotein cholesterol (HDL-C) and apolipoprotein (apo)A I level were significantly lower in the B1 homozygotes [(1.19±0.36) mmol/L vs (1.38±0.39) retool/L,( 1.17±0.33 ) g/L vs ( 1.30±0.31 ) g/L, P<0.05]. The B 1 homozygotes was associated with higher degree of cononary stenosis than the B2 carriers (P<0.05 ). There was no significant association between CETP-TaqIB genotype and the risk of CHD (P=0.147). Conclusions CETP-TaqIB polymorphism affects the concentrations of lipaproteins. There are significant associations between the B1 homozygotes and lowerHDL-C and apo A I levels. The B1 allele is not an independent risk factor for CHD.

BACKGROUND: With wide application of biotechnological substitute materials, pelvic repair and reconstruction develop to a certain degree. Biomaterial patch is a major substitute for repairing injured pelvic fascia tissue, so it is widely used for pelvic reconstruction.OBJECTIVE: To investigate the feasibility, efficacy, and clinical value of biomaterial patch to pelvic reconstruction in the females.METHODS: Articles related to pelvic functional disorder, pelvic reconstruction, and application of patch implant were retrieved from PubMed (http://www.ncbi.nlm.nih.gov/PubMed) and (http://www.wanfangdata.com.cn) with the key words of "reconstruction of whole pelvic floor, mesh, synthetic mesh implants" in both Chinese and English between 1990 and 2008. Duplication studies were excluded. A total of 54 articles were initially retrieved, and 17 ones were included in the final analysis.RESULTS AND CONCLUSION:Pelvic organ prohpse, which was a major symptom of pelvic disorder in the females, caused by defect of pelvic supporting structure, injury, and functional disorder. Traditional operation could not solve fundamental question.At present, substitute materials for pelvic repair and reconstruction mainly include biomaterial patch (self-substitute materials, homogeneity substitute materials, and heterogeneity substitute materials) and artificial patch. All of them could substitute the injured pelvic fascia tissue; therefore, they were major substitute materials of pelvic tissue and widely used for pelvic reconstruction. Patch which was used for pelvic reconstruction realized the recovery of anatomic structure and caused functional recovery, with simple and easy processing. Additionally, patch application did not prolong operative time and cause complication, but induced well tolerance, security and reliability, and remarkable short-term effect on patients. However, the long-term efficacy should be further studied. The modified pelvic reconstruction is clinically valuable for patients with varying prolapsed sites.

The etiology of Crohn's disease is not clear as yet,many evidences has shown that the dysfunction of the immune system is the internal cause of inflammation and tissue damage.It is identified that many immune diseases can be anticipated,monitored and therapeutic through detecting some specified serological markers.To identify some high specific sensitive and predictable markers and groups,the research of searching an ideal and non-incursive method for differentiating inflammatory bowel diseases from other disease is still undergoing.The current research of five serum immune markers that are usefulness in the diagnosis and management of Crohn's disease are summarized.

Objective To reveal the influence factor of ampD specific mutation on the transformation of AmpC β-lactamase from non-high-productive type to derepressed the high-productive type in Enterobacter cloacae. Methods The selected cbromatin-mediated derepressed high-productive strains of Enterobacter cloacae and genomic DNA were extracted, and genomic ampD was amplified by using PCR, and then sequenced and confirmed by specific mutation. Use site-directed mutagenesis to mutate the wild-type Enterobacter cloacae for the above site. The changes of AmpCβ-lactamase types were detected by the improved Cefotaxime three-dimensional test. Results Of the 121 strains of Enterobacter cloacae,15 strains were ehromatin-mediated derepressed high-productive type,8 strains had significant mutations and 7 strains of site-directed mutagenesis were established. Ecl MA(274 inser-ting A)、Ecl MC(327 missing C)and Ecl MF(27 inserting G)were changed from non-high-productive type to derepressed-high-pro-ductive type,but Ecl MB(371 inserting T), Ecl MD(515 missing C), Ecl ME(324 C→A), Ecl MG(238C→A 302T→A)did not change in AmpC β-lactamase types. Conclusion The significant mutations that could lead to the transformation of AmpC β-lacta-mase were the Frame-shift mutations that could create the change of large region of amino acid. However, other mechanisms for the derepressed high-productive AmpC β-lactamase were not excluded in the study.

In this study, biological activities of the lipopolysaccharide (LPS) extracted from Bacteroides fraglis NCTC 9343 were measured in animals. The results showed that LPS expressed typical endotoxicities, e.g. pyrogenicity in rabbits, lethality in mice and chick embryoes, ability to induce local Shwartzman reaction and coagulability for limulus emoebcyte lysate. But its biological activities were lower compared with that of Enterobacteriaceae. It is suggested that the weaker biological activities are related to the unusual chemical structure of the LPS.

The diagnosis of post-concussion symptoms (PCS) has been a controversial issue because of that its pathogenesis is often involved in both neural damage and psychosocial factors. Recent researches have examined the diagnostic and evaluating value of several available methods or tools, including clinical interview, conventional CT and magnetic resonance imaging (MRI), neuropsychologic testing and neurochemical test. In mild traumatic brain injury (MTBI), there exist diffusive damages to white matter distributed particularly near the gray matter-white matter boundary and in the long white-matter tracts in midline structures. The conventional imaging techniques are unable to detect any positive evidence due to the diffusive nature of these injuries. This paper is to review epidemiologic study, diagnostic criteria and relevant disputes, conventional diagnostic strategy and new MRI techniques, and then evaluate the application and prospective value of them in the diagnosis and rating of PCS and MTBI. Non-invasively quantitative assessments of modern brain imaging technique may be developed to sensitive and promising tools in the diagnosis and forensic evaluation of PCS.

OBJECTIVE To investigate the clinical and pathological characteristics and its diagnosis and treatment of the parotid gland tumor in children.METHODS The clinical data of 77 children with parotid gland tumor treated in Department of Head and Neck,Tianjin Cancer Hospital affiliated Tianjin Medical University from Dec.1985 to Dec.2007 were retrospectively studied.RESULTS Among the 77 cases,51 cases were benign tumor(66.2%),26 cases were malignant tumor(33.8%).Among the benign cases,hemangioma and lymphangioma were the most common diseases,which accounted for 37.3%(19/51).Mucoepidermoid carcinoma(34.6%, 9/26) was the most common malignancy.Sixty seven cases were treated surgically.Among 18 cases of the epithelial malignant tumors,7 cases treated combined with postoperative radiotherapy.During the period of following-up from 1 to 17 years,1 case of pleomorphic adenoma,1 case of malignant myoepithelioma and 1 case of mucoepidermoid carcinoma recurred. CONCLUSION The clinical and pathological features of parotid gland tumors in children were different from those in adults.The hemangioma,lymphangioma and teratoma are rare in adults but common in children.Half of the solid tumors of parotid gland in children are malignant,but only 23.7%in adults. The primary radical operation has great value to the prognosis of children parotid tumor.Although postoperative radiotherapy can reduce the risk of recurrence,oncogenesis in thyroid gland and the adverse effect on the orofacial development of radiotherapy in children need investigated clinically in future.

BACKGROUND:The essence of cell differentiation is a selectively intra-cellular gene expression,which results in specific proteinic synthesis and causes changes in biochemistry,structure and function.Thus,original proteomics and a single protein analysis can not meet the requirement in study.Proteomics technology provides a powerful tool due to the large scale,systemical study of protein transformation and interaction,which can be used for exploring molecular mechanism of bone mesenchymal stem cells(BMSCs)during directional differentiation.OBJECTIVE:To introduce proteomics,to summarize the research of proteomics in directional differentiation of BMSCs,and to forecast the development of proteomics research methods.METHODS:To search articles highly related with BMSCs,cell differentiation,and proteomics published on CNKI (www.cnki.net/index.htm),Sciencedirect(http://www.Sciencedirect.com),I.S.I(http://www.isiwebofknowledge.com)were searched,and the key achievements were included in the analysis.RESULTS AND CONCLUSION:A total of 29 documents were reviewed,and the experiences in the application of proteomics technology in the directional differentiation of BMSCs were summarized.With the innovation and development in methodology and technology,proteomics will become a powerful tool for us to study the potential mechanisms of BMSCs directional differentiation.

Objective To investigate the influence of plasmid spread and ampD mutation to Enterobacter cloacae that leads to the AmpC β-lactamase change from inducible type to constitutive type. Methods The Enterobacter cloacae were isolated from the patients with nosocomial infection. The inducible type isolations and their constitutive type changers were put into the same group. The plasmid ampC gene and chromatin ampD gene in pairs in each group were amplified, sequenced and compared. Results Of 195 patients infected by Enterobacter cloacae of inducible type, 25 (12.82%) were changed to the ones of constitutive high type. In these 25 changed groups, 10 were caused by plasmid spread, 10 by ampD mutation, 1 by both, and 4 by neither. Twelve changed constitutive type strains had ampD significant mutations, in which 7 were frame-shift mutations and 5 were spot mutations. Conclusions The change ratio of Enterobacter cloacae from inducible type to constitutive type is rather high. Both plasmid spread and ampD mutation are possibly the mechanism of such change. Plasmid mediated AmpC β-lactamase spreads among different species and interregionally. The mutation rate of chromatin ampD gene is also higher than the natural mutation rate. These two mechanisms should be considered in clinical treatment.