Objective:To study the surface enhanced Raman scattering ( SERS) of safflower and identify safflower injections by SERS quickly and effectively. Methods:Through comparative analysis of the Raman spectroscopy of safflower injections and the corre-sponding control herbs, the rapid identification of safflower injections was realized. Results:The results showed that several character-istic peaks of safflower were enhanced obviously in SERS, which could be used to identify safflower injections. Conclusion:The meth-od is reliable, rapid, accurate and specific, which can be applied as a method to identify safflower and its injections.

Animals ; Cattle ; Humans ; Infant ; Infant Formula ; Milk Hypersensitivity ; Milk Proteins ; adverse effects

The effects of monoterpene perilly alcohol (POH) alone or in combination with STI571 on the proliferation and apoptosis of the cell line K562 positive for Bcr/Abl were investigated. By using cell culture, the effect of the drugs on the proliferation of the cells was studied. TUNEL and flow cytometry assay of FITC-Annexin V and PI labeled cells were applied to detect the effects of the drugs on the apoptosis of the cells. The results showed that at 36 h, IC50 of POH on K562 positive for Bcr/Abl and HL-60 negative for Bcr/Abl were 81.0 +/- 11.3 micromol/L and 113.6 +/- 23.4 micromol/L respectively (P>0.05). POH could inhibit the proliferation of K562 in a time- and dose-dependent manner with the inhibitory rate of 100 micromol/L POH on K562 cells at 36 h being (53.2 +/- 3.65)%. K562 cells were more sensitive to STI571 than POH. IC50 of STI571 on K562 cells in 36 h was (0.256 +/- 0.054) micromol/L. In a time- and dose-dependent manner, POH induced the apoptosis of K562 cells with the percentage of apoptotic cells by 100 micromol/L POH at 40 h being (21.0 +/- 3.3)%. Both 100 micromo/L POH and 0.2 micromol/L STI571 had the same inhibitory effects on the K562 cells at 36 h. But at 12 and 24 h, the inhibitory rate of POH was significantly higher than that of STI571 (P<0.05) and the ability of STI571 inducing apoptosis at 36 h was greater than that of POH. 50 micromol/L, 100 micromol/L and 200 micromol/L POH in combination with 0.2 micromol/L STI571 could obviously increase the inhibitory effects on the cellular proliferation. Combined use of 50 micromol/L, 100 micromol/L, 200 micromol/L with 0.2 micromol/L STI571 could strongly induced apoptosis, especially 200 micromol/L POH in combination with 0.2 micromol/L STI571. It was concluded that the antileukemia effect of POH had no obvious Bcr/Abl positive selectivity. POH can inhibit the proliferation of K562 and induce the apoptosis in a time- and dose-dependent manner. K562 cells were more sensitive to STI571 than POH. POH in combination with STI571 could obviously enhance the abilities of STI571 inhibiting the proliferation and inducing apoptosis of K562 cells.
Antineoplastic Agents/*pharmacology ; Apoptosis/*drug effects ; Dose-Response Relationship, Drug ; Drug Synergism ; Fusion Proteins, bcr-abl/analysis ; HL-60 Cells ; K562 Cells ; Monoterpenes/*pharmacology ; Piperazines/pharmacology ; Pyrimidines/*pharmacology

Objective To compare the clinical features of myocardial infarction (MI) in young adults with and without diabetes. Methods A total of 94 young adults with MI were enrolled in this study and divided into two groups according to whether they had diabetes:diabetic group (DM) and Non diabetic group (NDM).The clinical features,angiographic results,risk factors and prognosis were compared between the two groups. Results The incidence of atypical chest pain (25.0% vs 7.6%),non-ST segment elevation myocardial infarction (50.0% vs 9.1%),ventricular wall motion abnormalities (78.6% vs 42.4%),double vessels (35.7% vs 21.2%),triple vessels (39.3% vs 3.1%) and the incidence of mortality(10.7% vs 1.5%)were all significantly higher in DM group than in NDM group (P<0.05 or P<0.01).The number of affected vessels and Gensini scores were (2.110±0.875) and (52.61±10.47) in DM group,and(1.140±0.677),(34.02±10.24) in NDM group.The between group difference was statistically significant (P<0.05).Multiple linear regression showed that HbA1c and smoking were the independent risk factors for Gensini score. Conclusion The clinical features of young adult patients with MI and diabetes are more complex,their coronary stenosis are more serious,and the prognosis is poor.Smoking and HbA1c are positively associated with Gensini scores.

Objective To investigate the clinical efficacy of psychological intervention combined with valsartan and Benner Pury in the treatment of congestive heart failure (CHF). Methods 100 patients with congestive heart failure treated in our hospital from January 2015 to October 2016 were selected and randomly divided into the control group and the experimental group, with 50 patients in each group. Patients in the control group were treated with Benner Pury, the experimental group was given valsartan and Benner Pury combined treatment, and psychological intervention, pay attention to the psychological state of patients. The clinical indexes of the experimental group and the control group were compared and analyzed. Results After the corresponding treatment, the effective rate of treatment in the experimental group was 96%, and the effective rate of the control group was 82%. The effective rate of the patients in the control group was significantly lower than that in the experimental group, with statistical difference (P<0.05). The incidence of adverse reactions in the experimental group was 8%, and the fatality rate was 10%. The incidence of adverse reactions in the control group was 10%, and the fatality rate was 12%. There was no significant difference between the experimental group and the control group in the incidence of adverse reactions and mortality, and it was not statistically significant, and could be compared. Conclusion The clinical effect of psychological intervention combined with valsartan in the treatment of congestive heart failure, Benner Pury good, can improve the treatment efficiency to a certain extent, high safety, is further applied in clinical significance.

Recently,paclitaxel has been used as a new agent on patients with advanced esophageal cancer,and several studies have demonstrated that paclitaxel and paclitaxel-based regimens have significant activity in patients with advanced esophageal cancer and showed highly response rates than the combination of 5-fluorouracil (5-Fu) and cisplatin.However,there have been some problems which need to be solved such as drug-resistance,side effect,and so forth.The new medicament form of paclitaxel can not only depress poison without anti-allergy but also raise medicament curative effect as well as the pharmacal concentration.Although most of the research on the new medicament form of paclitaxel are still limited in the stage of laboratory,it has possessed potential advance in the treatment of advanced esophageal cancer.

The severity of coronary artery diseases is assessed based on morphological and functional stenosis.The study Oil detection of coronary artery diseases based on morphological lesions has unveiled its short comings for evaluation of the seriousness of the disease.Therefore,many research groups diagnose the coronary artery diseases by functional stenosis.From functional aspect,studies of x-ray angiography algorithms for blood flow and velocity measurement are potentially clinically important and are an actively researched subject,which Can accurately diagnose the coronary diseases from functional stenosis aspect.Based on the angiographic images,the article describes formulation and characteristics of existing coronary blood flow and velocity techniques such as tracking and computational methods.Tracking methods can be divided into four classes:bolus transport time algorithms,continuous velocity determination from two-dimensional(time-distance)parametric images,contrast traversal distance algorithms,and droplet tracking algorithms.Computational methods can be divided into six classes:indicator-dilution or Stewart-Hamilton method,first-pass distribution analysis,inverted continuity equation algorithms,inverse mass transport problem approach,optical flow type algorithms,and the fluid continuity method.

Objective To evaluate the effectiveness of music therapy on anxiety and adverse reactions induced by chemotherapy in breast cancer patients after modified radical mastectomy. Methods One hundred and ten breast cancer patients were divided into intervention group and control group. The patients in the intervention group received music therapy,progressive muscle relaxation and guided inner image in addition routine nursing care. State Anxiety Inventory and Rotterdam Symptom Checklist (RSCL) were administered before the 2nd chemo-cycle and before the 3rd chemo-cycle. Results There was trend of lower scores on the Rotterdam Symptom Checklist total score and the State Anxiety score in intervention group than in control group (P＜0.05). Conclusion Music relaxation therapy can alleviate chemotherapy related adverse reactions and anxious.Therefore,music relaxation therapy is a practical psychological behavioral intervention for the breast cancer patients during the chemotherapy after modified radical mastectomy.

Objective Statistics and analyze the effect of comprehensive prevention measures for the ventilator associated pneumonia (VAP) morbidity and mortality,and investigate its actual applicate situation in the clinical.Methods Statistics and analyze the clinical date of 183 cases of our ICU mechanical ventilation patients in 2012 (group A),including morbidity,mortality,time of endotracheal intubation,length of stay and the primary pathogenic bacteria,and to compared with the relevant clinical data of 192 cases of our ICU mechanical ventilation patients in 2011 (group B),summarize and analysis the actual applicate situation of the comprehensive prevention measures in the clinical through the data contrast.Results The incidence of VAP in group A was lower than that in group B [31.7％(58/183) vs.58.3％(112/192)] (P ＜ 0.05),but the mortality was no statistical difference in both groups (P ＞ 0.05) ; the endotracheal intubation time and the average length of stay in group A were less than those in group B [6.3 d vs.10.6 d,7.6 d vs.11.9 d](P＜0.05); the main pathogenic bacterium of VAP with mainly gram-negative bacilh.Conclusions Comprehensive prevention measures can effectively reduce the incidence of VAP,but the mortality rate has no significant effect,need further clinical research;comprehensive prevention measures to reduce patients with tube time also confirm its effectiveness.Comprehensive prevention measures are economical and practical,is worth in clinical promotion.

Objective To observe the effects of Uremic Clearance Granule combined with angiotensin receptor blockers (ARB) or angiotensin converting enzyme inhibitors (ACEI) in treating different period of diabetic nephropathy (DN ) .Methods 46 patients with DN were divided into the control group and the treatment group ,23 cases in each group .The two groups were given the rou-tine hypoglycemic therapy .The control group was treated with ACEI or ARB ,while the treatment group was given Uremic Clear-ance Granule 5 g each time ,four times daily for successive 4 weeks .Then the indexes of serum creatinine ,blood urea nitrogen ,cysta-tin-C ,microalbumin and 24 h urine total protein at 24 h before and after treatment were observed .Results The microalbumin and 24 h urine total protein after treatment in the two groups were reduced compared with before treatment (P<0 .01) .In the patients with the stage Ⅲ of DN ,the urine protein decrease after treatment had no statistical difference between the two groups (P>0 .05) . In the patients with the stage Ⅳ of DN ,on the basis of ACEI or ARB adding Uremic Clearance Granule could significantly decrease the urine protein(P<0 .05) .Conclusion ACEI or ARB combined with Uremic Clearance Granule has definite effect for treating massive proteinuria of DN .