Objective To compare the clinical effect of hypertensive intracerebral hemorrhage treated by small bone window craniotomy or craniotomy.Methods 192 cases of patients with hypertensive cerebral hemorrhage treated were selected and divided into minimally invasive group and flap group randomly.Small bone window craniotomy was used in minimally invasive group,while large trauma craniotomy was used in flap group.Complications,rebleeding and short and long term efficacy were recorded and compared.Results 6 cases(6.3％) of.incidence of complications in minimally invasive group,while 14 cases(14.56％) in flap group,the rate in the former group was lower than that in the latter group (x2 =51.308,P ＜ 0.05).There was no significant difference of rebleeding between the two groups (P ＞ 0.05).3 weeks later,GCS score was (10.9 ± 1.8),GOS score (3.3 ± 0.6),BI index (47.8 ±11.5) and 64 cases' nerve function improved (66.7 ％) in minimally invasive group,while GCS score (10.1 ± 2.5),GOS score(2.7 ±0.8),BI index (33.5 ± 15.4) and 31 cases' nerve function improved(32.3％) in flap group.The GOS score,BI index and the situation of nerve function improvement were better in minimally invasive group than that in flap group(t =15.758,56.529,x2 =72.149,P ＜0.05).None of the patients were dead,and there were no significant differences of GCS score between the two groups(P ＞0.05).6 months later,no significant difference of GCS score and mortality were found between the two groups (P ＞ 0.05).but the mean score of GOS and BI index were higher than those in flap group (t =11.647,32.990,P ＜ 0.05).Conclusions Both large trauma craniotomy and small bone window craniotomy could reach therapeutic purposes.However,intraoperative and postoperative complications could be deceased by small bone window craniotomy,and short and long term GOS score and BI are higher,the short term nerve function improvement is better.

Objective To study the significance and function of the comprehensive weight in multiple linear regression analysis of complex random sampled data .Methods Based on the concept of Monte Carlo random simulation , two different multiple linear regression analysis procedures in SAS-REG and SURVEYREG were used to perform regression modeling for the same batch of complex random sampled data ( n=6756 ) at different random sampling proportions .The results were compared.Results In the results of the fitting multiple linear regression model when observation weight and sampling weight were considered or not , it was found that the size of the partial regression coefficient , standard error and P value of independent variables varied .Conclusion In complex random sampled data based on different proportions ,especially in regression modeling of stratified random sampling survey information , the multiple linear regression model makes it possible to more accurately and sensitively perform parameter estimates of regression coefficients and statistical prediction of outcome variables if the comprehensive weight of the survey data is incorporated into the statistical analysis .

Early detection of HCC is critical for a good prognosis. Therefore, the development of tumor markers that can detect HCC at even earlier stages is urgent. Recent researches show that the human cervical cancer oncogene, gamma-glutamyl transferase mRNA, human telomerase reverse transcriptase mRNA, the proteins such as glypican-3, golgi protein 73, vascular endothelial growth factor and transforming growth factor-β1 could serve as markers for early detection of HCC.

Epstein-Barr virus infects widely, and has a powerful ability to transfrom into a number of malignant diseases. The infection is mostly latent, without any obvious clinical symptoms. At present, more and more researches have found that cell-mediated immunity,especially T lymphocyte response plays an important role in the control of Epstein-Barr virus infection.

Objective In order to know the application effects of transcatheter hepatic artery chemoembolization for patients with liver cancer. Methods Divided 144 liver cancer patients into the experimental group and the control group randomly, there were 82 cases in each group. Routine nursing cares were used in the control group, humanized nursing were used in the experimental group in addition. Compared the curative effect, incidence rate of complication and scores of SAS and SDS between the two groups. Results The curative effect in the experimental group was significant better than in the control, the incidence rate of complications were lower in the experimental group than those of in the control group. Before out of hospital, the SAS and SDS level in the experimental group was better than in the control group. Conclusions Humanized nursing can enhance the curative effec4 avoid disputs between patients and nurses, and then promote nurses' working interesting.

A Strategy was established for construction of repertoire antibody library with affinity chromatography purifying antigen, antigen immunizing human lymphocytes, RT-PCR and phage display technology. The colorectal carcinoma-associated antigen CA-Hb3 was purified with affinity column and analysed with SDS-PAGE and Western-blot, then applied for immunizing peripheral blood lymphocytes (PBL) in vitro. The PBL were isolated from ten patients with colorectal carcinoma and cultured with interleukin 2 and pokeweed mitogen, lymphoblast-like cells occurred and colonies formed after immunization. Three VH-CHl(?) and five VL-CL(?,?) genes were amplified from their total RNA and mRNA with RT-PCR. Three VH (?) and 8 VL (?,?) genes were reamplified and randomly combined to construct 24 single-chain variable fragments (ScFv) genes through (Gly_(4)Ser)_(3) linker. ScFv genes digested with Sfi I were cloned into fUSE 5 vector and transformed into MC1061 with electroporation. Repertoire antibody library was obtained with 10~(6) tetracycline-resistant colonies, in which the percentage of ScFv inserts was 85 % . This strategy might be used for humanizing mouse-original monoclonal antibody.

Objective:To investigate the effect and safety of sufentanil combined with propofol for intravenous anesthesia in chil-dren undergone painless gastroscopy. Methods:Totally 120 cases of children undergone painless gastroscopy were divided into the ob-servation group (60 cases) and the control group (60 cases) according to the random number method. The observation group was given sufentanil combined with propofol for anesthesia, and the control group was given fentanyl and propofol for anesthesia. The hemodynam-ic indices at the period of T0 (before the anesthesia), T1 (1 min after the anesthesia induction), T2 (checking), T3 (2min after the checking) and T4 ( at the end of testing) were compared between the groups, and the anesthesia situation, hospitalization time, dosage of propofol, fine/excellent rate of anesthesia and adverse reactions were also observed. Results:In T1, T2 and T3, the MAP ( mean arterial pressure) , HR ( heart rate) and RR ( respiratory rate) were declined in the two groups when compared with those in T0 ( P<0. 05), and those in the observation group were higher than those in the control group (P<0. 05). The time of consciousness disap-pearance, eyes open, orientation force recovery and hospitalization in the observation group was shorter than that in the control group, and the propofol dosage in the observation group was lower than that in the control group(P<0. 05) . The excellent number and rate of the observation group were better than those of the control group (P<0. 05), and the incidence of apnea of the observation group was lower than that of the control group (P<0. 05). Conclusion: Sufentanil combined with propofol for anesthesia in children undergone painless gastroscopy has the properties of fast onset and recovery, stable hemodynamic indices during the operation and high safety, which is worthy of promoted application.

Objective To analyze post-traumatic growth of Chinese caregivers of dementia patients and to explore the caregivers′positive and potential energy, in order to formulate more perfect nursing intervention measures. Method Useing phenomenological method of qualitative research in selecting 6 dementia caregivers in-depth interviews to understand possible posttraumatic growth. Result Five major themes were found: changes in individual cognitive, changes in relations with others, promote their children′s education, reflections and concerns on status of pension-related changes in philosophy of life. Conclusions Dementia caregivers also have the positive performance of posttraumatic growth, in addition to the negative experience. Clinical nurses should actively explore the potential and power from the growth perspective. This is conducive to the caregivers with more health caring for life, so as to improve the quality of care, and ultimately improve the quality of life.

Glutathione S-transferase kappa 1 (GSTK1) is a key regulator for adiponectin secretion and multimerization.In Caenorhabditis elegans,GSTK1 is involved in energy production and lipid metabolism.Meanwhile,the GSTK1 level is negatively correlated with obesity.It may alleviate the endoplasmic reticulum stress-mediated downregulation of adiponectin.Moreover,a polymorphism in human GSTK1 promoter is related with insulin secretion and fat deposition.Therefore,GSTK1 might be a novel target for the treatment of insulin resistance and the relevant metabolic diseases.

Hepatitis B virus (HBV) infection is a global public health issue,especially in the Asian-Pacific region.At present,entecavir,tenofovir,and pegylated interferon are the first-line antiviral drugs recommended by related guidelines for the prevention and treatment of hepatitis B;however,different national/regional policies,economic level,coverage of health insurance,and medical level have caused the difference in antiviral regimens.Therefore,we have established a long-term follow-up cohort of hepatitis B to evaluate the efficacy of different treatment regimens,in order to improve the cure rate of hepatitis B and reduce the incidence rates of liver cirrhosis,liver cancer,and end-stage liver disease.Real-world data from cohort study help to understand the differences between guidelines and clinical practice,provide a reference for HBV guidelines,and balance clinical effect and cost-effectiveness.