MicroRNAs (miRNAs) are a class of small,non-coding,single-stand RNA molecules which are composed of 21-25 ribonucleotides.They regulate gene expression at post-transcription and participate in many important biological processes including cell growth,apoptosis,viral infection and cancer development.At present,the studies on miRNAs in cervical lesion include miRNAs expression characteristic of cervical cancer and precancerous lesion,the association with HPV infection and prognosis of cervical lesion,cervical cancer development,diagnosis and therapy.

Tooth extraction is a common operation in oral surgery. Traditional-extraction instruments, such as bone chisel, elevator, and bone hammer, lead to not only severe trauma but also unnecessary complications, and patients easily become nervous and apprehensive if tooth extraction is performed using these violent instruments. In recent years, with the develop- ment of minimally invasive concept and technology, various micro-power instruments have been used for tooth extraction. This innovative technology can reduce the iatrogenic trauma and complications of tooth extraction. Additionally, this technology can greatly decrease the patient's physical and mental pressure. The new equipment compensates for the deficiency of traditional tooth extraction equipment and facilitates the gradual replacement of the latter. Diverse micro-power systems have distinct strengths and weaknesses, so some auxiliary instruments are still needed during tooth extraction. This paper focuses on the various micro-power systems for tooth extraction and tries to compare the advantages and disadvantages of these systems. Selection and usage of auxiliary equipment are also introduced. Thus, this paper provides reference for the proper application of the micro-power systems in tooth extraction.
Humans ; Tooth Extraction ; instrumentation

Objective To investigate the value of ultrasound in diagnosis of fetal heart malformation at 11-13+6 weeks gestation.Methods A total of 70 pregnant women were selected,based on the results of induction of labor and postnatal imaging examination, the effect of ultrasound on fetal heart abnormalities in 11-13+6 weeks was observed.The display of cardiac malformations with different cuts was compared.Results In 70 fetuses with cardiac malformations, malformation types included tetralogy of Fallot, double outlet right ventricle, complete endocardial cushion defect, transposition of the great arteries, persistent truncus arteriosus, aortic arch, venous drainage.Ultrasound diagnosis of 64 cases of fetal cardiac malformations, the diagnostic accuracy rate was 91.4%.Among them, the highest proportion of tetralogy of Fallot (42.9%), venous drainage was the lowest (2.9%).The diagnostic rate of 4CV+3VVT ultrasound for fetal heart malformation was the highest(91.4%), which was significantly higher than 4CV+VOTV(72.2%), 4CV+(AR-SAV)(68.6%) and 4CV, the differences were statistically significant (x2=7.643,5.324,4.447,all P<0.05).The diagnostic accuracy of 4CV+3VV ultrasound for fetal heart malformation was 88.6%, which was significantly higher than 4CV+ (AR-SAV) and 4CV section, the differences were statistically significant (x2=6.343,4.011,all P<0.05).Conclusion Ultrasound can be used to detect fetal heart malformation at 11-13+6 weeks gestation, among them, the accuracy of 4CV+3VVT ultrasound section is the highest.

Objective To investigate the prevalence of nonalcoholic fatty liver disease in a population of routine check-up,and analyze the correlation between non-alcoholic fatty liver disease and metabolic risk factors.Methods Physical examination data of 2 064 routine check-up (aged 45 or more) in the Third People's Hospital of Chengdu from January 2014 to December 2015 were randomly selected.HbA1c,fasting blood glucose,blood lipids and other indicators were measured to calculate the prevalence of NAFLD,and to analyze the correlation between NAFLD and metabolic risk factors.Results Among 2 064 cases underwent physical examination,the overall prevalence of NAFLD was 16.09％ (332/2 064).The male prevalence rate of NAFLD was significantly higher than that in female (18.51％ vs.12.87％;x2 =11.913,P =0.001).In four age groups (＜ 50 years old,50 to 59 years old,60 to 69 years old,≥ 70 years old),the NAFLD prevalence rate was 16.26％,15.63％,16.97％ and 14.72％ respectively.No statistical significance (x2 =0.900,P =0.826) was found in NAFLD prevalence rate difference in all age groups of has.In 50 ～ 59 years old and ≥70 years old group,the NAFLD prevalence rates in male were higher than that in female,the difference was statistically significant (both P ＜ 0.05).BMI,waist circumference,systolic blood pressure,diastolic blood pressure (DBP),HbA1c,fasting blood glucose,TC,TG,LDL-C,uric acid levels in NAFLD patients were higher than the normal population.HDL-C levels in patients with NAFLD is lower than that of the normal,and the difference is statistically significant (both P ＜ 0.05).Logistic regression analysis showed that BMI,hypertension,diabetes,and high TG were the independent risk factors of NAFLD;HDL-C and NAFLD were the protective factors.Conclusions In the Chengdu area of Sichuan Province,the prevalence rate of NAFLD was high,the incidence rate of NAFLD in male was higher than that in female.BMI,hypertension,diabetes mellitus and high TG were the independent risk factors of NAFLD,and HDL-C was the protective factor.

That provincial hospital hosting the local hospital is an important measure to deepening the reform of medical and health system.Advanced hospital management mechanism and excellent technical experts were gradually introduced into the local hospital to establish South Hubei healthcare alliance,and form province-city-county healthcare alliance.In this way,high quality management,manpower and technology resource sink,overall medical technology level achieves good breakthrough.This promotes the rapid development of healthcare alliance member hospitals in the region.

Apolipoprotein M-sphingosine-1-phosphate axis ( apoM-S1P axis ) signaling pathway consists of apolipoproteinM (apoM), sphingosine-1-phosphate (S1P) and sphingosine-1-phosphate receptor (S1PR).Plasma apoM belongs to lipocalinsuperfamily members , and is mainly associated to high density lipoprotein( HDL), whereas HDL-cholesterol correlates inversely with cardiovascular risk .The ability of apoM to bind S1P is due to a lipophilic binding pocket within the lipocalin structure of the apoM molecule . S1P, a bioactive mediator of phospholipid metabolism , predominantly abound in HDL among all lipoproteins.S1P can not only be used as intracellular second messengers , but also as intercellular signal molecules, activating of G protein-coupled receptors (S1PR) to mediate various physiological functions.It′s clear that apoM protects human beings from atherosclerosis .Furthermore, recent studies showed that S1P has a significant impact on atherosclerosis , and ApoM-S1P axis may play a important role in the pathogenesis or progression of atherosclerosis .

OBJECTIVE:To observe clinical effect and safety of temozolomide (TMZ) combined with radiotherapy in the treatment of malignant glioma. METHODS:58 patients with malignant glioma were randomly divided into TMZ-RT group(32 cas-es)and RT group(26 cases). RT group were given the therapy of 3D-CRT 1.8-3 Gy/d,and some patients received the hyperfrac-tion radiotherapy with total dose of 60-75 Gy. TMZ-RT group were given TMZ chemotherapy 75 mg/(m2·d)combined with radio-therapy for 6 weeks and TMZ sequential therapy 150-200 mg/(m2·d)for 4 weeks. Therapeutic efficacy and ADR were observed in 2 groups. RESULTS:After treatment,in TMZ-RT group,9 cases had complete remission,11 cases had partial remission,10 cas-es were stable and 2 cases were progressive;in RT group,6 cases had complete remission,8 cases had partial remission,8 cases were stable and 4 cases were progressive. There was no statistical significance in short-term efficacy between 2 groups(P>0.05). The 1,2,3-year survival rates of TMZ-RT group were 81.3%(26/32),78.1%(25/32) and 62.5%(20/32),while those of RT group were 84.6%(22/26),34.6%(9/26)and 15.4%(4/26). There were statistical significance in 2,3-year survival rates between 2 groups (P<0.05). Median recurrent time of TMZ-RT group was (23.2 ± 8.6) months,while that of RT group was (15.6 ± 8.7) months,with statistical significance(P<0.05). According to the KPS,TMZ-RT group was(89.6±9.6)and RT group(65.1±10.1), with statistical significance (P<0.01). The side effect of 2 groups were slight. CONCLUSIONS:Radiotherapy combined with TMZ can significantly improve the survival rates of 2 and 3-year and delay the tumor recurrent time,and lighter adverse reaction. It can be used as new therapy method for malignant glioma. But it needs further study for treating the malignant glioma patients with osteomyelitis or low immune function.

Objective To study the effect of community nursing and family members′participation on rehabilitation of patients with cerebral infarction. Methods Forty-two patients with cerebral infarction discharged from our hospital was chosen to take community rehabilitation training. The scores by modified Barthel index (MBI) and functional comprehensive assessment (FCA) before intervention were compared to those after intervention at the time points of pre-intervention and 1 month , three months after intervention. Results The scores by modified Barthel index (MBI) and functional comprehensive assessment (FCA) before intervention were significantly higher than those before intervention (P<0.01). The scores by MBI 1 month after intervention were significantly higher than those three months after intervention (P<0.01), indicating the longer the rehabilitation training, the better the effect. Conclusion The community rehabilitation care to patients with cerebral infarction sequelae can improve the quality of rehabilitation training and finally their quality of life.

Objective To explore the expression of Ang -2 and CD105 in breast cancer and their po-tential associations with the cancerization and progression of breast cancer .Methods Thirty patients with breast cancer ( cancerous tissue ) ,15 intraductal papilloma cases ( precancer tissue ) and 15 normal breast tissue were col-lected for each group ,respectively .Immunohistochemistry ,western blot and RT-PCR techniques were used to ex-amine the expressions of Ang -2 and CD105 in cancerous breast tissue ,precancer breast tissue and normal breast tissue at both protein and mRNA levels .The possible correlations of Ang -2 and CD105 expression with clinico-pathological characteristics of the breast cancer were analyzed .Results The expressions of CD105 and Ang-2 in cancer and precancer tissues were higher than in normal tissues at both protein and mRNA levels ,and the trend of their expressions was increased from normal to precancer ,and cancer tissue .The expressions of CD 105 and Ang-2 protein and mRNA were positively correlated with tumor size ,invasion,lymph node metastasis,and negatively correlated with the differentiation of the cancer .Conclusion CD105 and Ang-2 are involved in the canceriza-tion and pregression of breast cancer .These two biomarkers can serve as two useful indicators in assisting diagno-sis and prognosis of breast cancer .

The study was based on the maths model of waves which were reflected by a reflecting ellipsoid and whose distributing of energy in the ellipsoid. We developed an equipment by ellipsoidal focus to substitute for energy based on the research. The equipment has the characteristics of revolving therapy as the X-knife and the γ-knife which can be used in 3D therapy. We call the system ″Ultrasonic-knife″ which were used in 3D hyperthermia. The equipment simulates an source of energy by the ellipsoidal focus. The source can be put in the tumor in deep organ． It also avoids some shortcomings, just as heat is in limit area and temperature is too high in the focus. We could do an ideal conformal therapy by a reasonable treatment planning.