Background and purpose: Mouse osteosarcoma model was widely used in osteogenic malignant tumor research, and it was helpful for studying the invasion and metastasis of the tumor cells when it was well marked in vivo. The purpose of this study was to establish mouse sarcoma cell lines (S180) that were infected with enhanced green fluorescent protein(EGFP). Methods: EGFP-S180 expressing strong EGFP fluorescence was acquired by electroblot, and supplemented with G418 (800 mg/mL), c-Myc was detected by laser scanning confocal microscopy. Meanwhile, the cancer-bearing model was established subcutaneously within the abdominal cavity. Results: EGFP-S 180 cells were cloned. There was no significantly difference between c-Myc expressions in S180 cells and those in EGFP-S180 cells (P>0.05), and between the cancer-bearing time subcutaneously and the time within abdominal cavity (P>0.05). Conclusion: According to in vitro and in vivo assay, it showed that EGFP-expressing S180 cells could be used for studying further the tumor biological behavior with fluorescence technology.

Objective To explore the present state of clinical decision-making ability of nursing students in Sichuan Province. Methods The instrument for measuring the clinical decision-making ability of nursing students was adopted te investigate baccalaureate nursing students from four colleges and uni-versifies in Sichuan Province who had completed 10-12-month clinical practice. Results The overall mean score of clinical decision-making ability of baccalaureate nursing students in Sichuan Province was (72.86± 4.53). Among the dimensions, the scoring rate of Goal-setting Ability Dimension was the highest and that of evaluation-feedback ability dimension the lowest. In terms of the qualification rate of clinical decision-making ability, 99.52% of the students were qualified (4.82% good and 94.7% passed) and 0.48% was unqualified. Conclusions Baccalaureate nursing students in Sichuan Province have not showed a high level in clinical nursing decision-making ability and most of them are just qualified. No students have reached the level of excellence.

Objective To evaluate the increased carotid artery pulsatility index(PI) and resistance index (RI) in young obese males by Doppler ultrasound.Methods All of the volunteers without local and systemic disease included 48 young obese males (observation group) and 100 healthy males with the same age(control group).Common carotid artery had to come into practice in all of the volunteer by pulsed Doppler ultrasound to calculate the PI and R[.Results The average of RI,PI,body mass index (BMI),systolic pressure,diastolic pressure,fasting plasma insulin,fasting C-peptide,triglyceride,low density lipoprotein cholesterol and high sensitivity C reactive protein in observation group were significantly higher than those in control group [0.70 ± 0.10 vs.0.60 + 0.10,1.48±0.30 vs.1.00 ± 0.10,(36.6 ± 3.4) kg/m2 vs.(23.6 ± 1.6) kg/m2,(127.5 ± 14.3) mm Hg(1 mm Hg =0.133 kPa) vs.(110.5 ± 11.0) mm Hg,(78.8 ±9.8) mm Hgvs.(69.9 ±7.6) mm Hg,(27.5 ± 19.6) mU/L vs.(8.8 +8.8) mU/L,(3.7 ± 1.6) pmol/L vs.(1.8 ± 1.2)pmol/L,(3.9 ± 2.0) mmol/L vs.(2.1 ± 1.3) mmol/L,(3.1 ± 0.7) mmol/L vs.(2.3 ± 0.7) mmol/L,(4.0 ± 4.4)mg/L vs.(1.3 ± 1.2) mg/L,P ＜ 0.05].Conclusions The characteristic of vascular properties in young obese males with normal blood pressure and without other cardiac risk factor is the decrease of blood wall compliance and the increase of resistance.The change of artery in the incipient properties in young obese males is the vascular function.Further studies will be required to prove thai whether early intervention will improve vascular function such as diet and practice.

The incidence of early neurological deterioration of acute ischemic stroke is higher and the clinical prognosis is poor.There are no effective prevention and treatment measures yet.The prognosis may be improved if early predicts by serum biomarkers and actively manages.This article reviews the serum biomarkers of early neurological deterioration in ischemic stroke.

Objective To explore the application value of nasobiliary duct instead of T-tube in primary suture after laparoscopic common bile duct exploration (LCBDE). Methods A total of 58 cases of choledocholithiasis without acutecholangitis were divided into experimental and control group according to the odevity of the last number of admission number .Patients in odd number belonged to experimental group , while the even number patients belonged to control group .The experimental group received endoscopic nasobiliary drainage ( ENBD ) combined with LCBDE and primary suture;the control group underwent LCBDE combined with T-tube drainage .The intraoperative and postoperative data were compared between the two groups . Results All surgeries were completed successfully under laparoscope .The experimental group had much shorter hospital stay than that of the control group [(7.5 ±2.1) d vs.(10.3 ±3.2) d,t=-3.965,P=0.000].No significant differences were found in the operative time , incidence of bile leakage and postoperative incision pain between the two groups (P>0.05).All the cases were followed up for 6-12 months (average, 9 months) and no patients were hospitalized due to complications related to the billiary surgery .MRCP showed no complications such as biliary stricture and residual stones 6 months after operation . Conclusions Nasobiliary duct can substitute for T-tube in LCBDE .It ensures the safety of the primary suture and reduces T-tube related complications .

Objective:To clone and express active domain of human granzyme A ( aGzmA ) and detect its biological activity.Methods:Human aGzmA gene was amplified by PCR from the full-length human granzyme A and inserted into prokaryotic ex-pression vector pET24a(+).The constructed recombinant plasmid pET24a-aGzmA was transformed to E.coli BL21(DE3) and induced with IPTG.The expressed product was identified by SDS-PAGE and Western blot.The recombinant protein was purified by the Ni2+affinity column chromatography and the enzyme activity was assayed with BLT substrate.Results: A DNA fragment of 700 bp was amplified by PCR.The recombinant plasmid pET24a-aGzmA identified by enzyme-digesting analysis and sequencing showed that aGzmA gene was inserted into vector plasmid correctly.SDS-PAGE analysis showed that there was a specific protein with a relative molecular mass of about 26 kD.Western blot analysis indicated that the protein could react with mouse anti-His monoclonal antibody specifically.The recombinant protein with high purity could be acquired from the inclusion bodies by the Ni2+ affinity column chromatography and the purified protein had good enzyme activity.Conclusion: The recombinant human granzyme A with good biological activity was prepared successfully.

Objective　To investigate the direct antitumor effect of several surface molecules of Bifidobacterium bifidum 1101 such as whole peptidoglycan (WPG), lipoteichoic acid (LTA), and polysaccharides (PS) on human colonic adenocarcinoma cell line LoVo. Methods　MTT assay and flow cytometry (FCM) were used to evaluate the effect of Bifidobacterial surface molecules on the growth of LoVo cells. Morphological study, fluorescent polarimetry and differentiation-specific enzyme analysis were used to determine whether LTA treatment could induce the differentiation of the cells. Results　LTA but not WPG or PS significantly inhibited the proliferation of LoVo cells in a dose and time dependent manner. Cell cycle analysis by FCM revealed that LTA induced changes in cycle distribution resulting in G0/G1 phase commitment. Morphological alterations observed by light and electron microscopy indicated maturity of cytoplasm and nucleus following treatment by 50μg/ml of LTA. Membrane fluidity was also altered, and intestinal alkaline phosphatase specific for the differentiation of LoVo cells roused dramatically during culture in LTA. It was further found that LTA elevated ［Ca2+］i which resulted from Ca2+ influx instead of the release of stored calcium. Conclusion　These observations indicated that LTA was able to inhibit the growth of LoVo cells and induce the differentiation of the cells. Calcium influx may play an important role in this process.

Objective To explore the ability of intestinal bacteria making use of creatinine in the patients with uremia and healthy subjects. Methods Creatinine, which was energy source only,and tetrazolium violet as indicator were added into intestinal bacteria of the patients with uremia and healthy subjects(n=10). It was positive that solution showed violet. Intestinal bacteria were added in Nutrient Broth medium with creatinine. The concentration of creatinine was measured before and after incubation. Results The solution of intestinal bacteria of the patients with uremia showed violet. The solution of intestinal bacteria of healthy subjects showed bright violet.The concentration of creatinine decreased after incubation in both groups. Conclusions The intestinal bacteria of uremic patients and healthy subjects had the ability to make use of creatinine. The former was stronger than the latter.

In order to understand the effect of oxidant and antioxidant on proteinuriaformation of rats with passive Heymann nephritis (PHN), vitamin E (VitE), malondialde-hyde (MDA), xanthine oxidase (XOD) in serum and urine, and 24 hours protein excretionin urine were measured. The results showed that in both rats of heterologous phase (day7) and autologous phase (day 14), MDA in serum and urine, VitE and, 24 h pro-tein excretion in urine were all markedly increased, while VitE in serum was significantlydecreased, however the XOD level in serum and urine were not much altered. Correlationanalysis revealed that there were positive correlation between the amount of urinary pro-tein and that of MDA in serum and urine, and also between that of urinary protein andVitE in urine. A negative correlation existed between the amount of urinaryprotein andVitE level in serum. These results suggested that in rats with PHN there be an hyper-lipidperoxchtion with an pypo-anti-oxidation.

The normal arterial patterns in the left ventricle wall of 100 rabbit hearts were studied by means of postmortem coronary angiography, the clearing method and histological sections, and in comparison with that of the human heart.The intramural arterial patterns in the left ventricle of rabbit heart are similar to human heart in many ways. "Branching" type arteries (Class A vessels) supply the whole myocardium, including the subendocardial zone, while "straight" type arteries(Class B vessels)mainly supply the papillary muscle and trabeculae carneae where they form the subendocardial plexus. The arterial type in papillary muscles may be classified into segmental pattern, axial pattern and mixed pattern. The arterial distribution in the interventricular septum is without rule. The capillaries in all areas run parallel with the myocardial fibres and show cross connexions somewhere.