The incidence of early neurological deterioration of acute ischemic stroke is higher and the clinical prognosis is poor.There are no effective prevention and treatment measures yet.The prognosis may be improved if early predicts by serum biomarkers and actively manages.This article reviews the serum biomarkers of early neurological deterioration in ischemic stroke.

Objective To evaluate the increased carotid artery pulsatility index(PI) and resistance index (RI) in young obese males by Doppler ultrasound.Methods All of the volunteers without local and systemic disease included 48 young obese males (observation group) and 100 healthy males with the same age(control group).Common carotid artery had to come into practice in all of the volunteer by pulsed Doppler ultrasound to calculate the PI and R[.Results The average of RI,PI,body mass index (BMI),systolic pressure,diastolic pressure,fasting plasma insulin,fasting C-peptide,triglyceride,low density lipoprotein cholesterol and high sensitivity C reactive protein in observation group were significantly higher than those in control group [0.70 ± 0.10 vs.0.60 + 0.10,1.48±0.30 vs.1.00 ± 0.10,(36.6 ± 3.4) kg/m2 vs.(23.6 ± 1.6) kg/m2,(127.5 ± 14.3) mm Hg(1 mm Hg =0.133 kPa) vs.(110.5 ± 11.0) mm Hg,(78.8 ±9.8) mm Hgvs.(69.9 ±7.6) mm Hg,(27.5 ± 19.6) mU/L vs.(8.8 +8.8) mU/L,(3.7 ± 1.6) pmol/L vs.(1.8 ± 1.2)pmol/L,(3.9 ± 2.0) mmol/L vs.(2.1 ± 1.3) mmol/L,(3.1 ± 0.7) mmol/L vs.(2.3 ± 0.7) mmol/L,(4.0 ± 4.4)mg/L vs.(1.3 ± 1.2) mg/L,P ＜ 0.05].Conclusions The characteristic of vascular properties in young obese males with normal blood pressure and without other cardiac risk factor is the decrease of blood wall compliance and the increase of resistance.The change of artery in the incipient properties in young obese males is the vascular function.Further studies will be required to prove thai whether early intervention will improve vascular function such as diet and practice.

Objective:To study the effects of the Gekko gecko ethanol extract on granular cells apoptosis of rat ovaries.Methods :3 month and 6 month female Wistar rats were taken in this study, and 20 rats were in each age group.Each age group were randomly divided into two groups(experimental group and control tragastric administration in experimental group, corresponding volume of 0.9% sodium chloride was given in control group by the same way.Apoptosis of granular cells and distribution of cell cycle in rat ovary were measured by terminal-deoxynucleotidyl transferase mediated d-UTP nick end labeling (TUNEL) and propidi-um iodide(PI) staining, AnnexinV/PI double labelling staining by flow cytometry (FCM) after administration for 15 days.Re8utts:The apoptosis numbers of granular cells in 3 month and 6 month age experimental group were 34 ±7 and 53 ±11, in control group were 48±9 and 76±17 respectively by TUNEL.There was signrficantly statistic difference (P <0.01).The apoptosis rates in 3 month and 6 month age experimental group were(6.06 ±2.01)% and (12.16 ±3.26) % , in the control group were (8.23±2.13) % and (23.69 ±6.28)% respectively by PI staining.There was significantly statistic drfference (P <0.05).However, no significant difference was found between two groups in cellular cycle distribution (P > 0.05).Early apoptosis rates in 3 month and 6 month age experimental group were (6.71±3.11) % and (23.74±6.28) % , in the control group were (19.05 ±5.78) % and (36.55± 8.35) % respectively by AnnexinV/PI double labelling staining FCM.There was significantly statistic difference (P <0.01).The apoptosis of granular cells were sig-nificantly less in experimental groups comparing wrth control groups both in 3 month and 6 month age group (P<0.01 or P<0.05).Conclusions :The ethanol extract of gekko gecko can inhibit the apoptosis of granu-lar cells in rat ovary, then improves the ovary function and delay ageing of rat.

Objective To study preliminar il y the mutual antigens between Lumbricus terrestris[WT5”BZ ] and Schistosoma japonicum. Methods The antigen s of Lumbricus terrestris (L.terrestris) and [ WT5”BX]Schistosoma japonicu m (S.japonicum) were analysed by SDS-PAGE technique, and reacte d with the sera of patients with schistosomiasis and the sera of rabbits immunized with LtAg by Dot-ELISA and Western-blot. Results [WT5” B Z]There were many similar molecular weight antigens between LtAg and SjAg. Spe cially some antigens between 30-90 kDa form LtAg had intensive cross reactio n with the sera of patients with schistosomiasis. SjAg was also reacted with the sera of r abbits immunized with LtAg. Conclusion There are at least three immunocompetent antigens with similar molecular weight between L.terrestris and S.japonicum. [

OBJECTIVE: To comprehend the general situation of pharmaceutical services in hospitals in China.METHOD: The general situation of pharmaceutical services was investigated by questionnaire survey through mailing among a total of 600 hospitals including third-level grade A and B and second-level grade A and B hospitals and 42 pilot sites hospitals of clinical pharmacist work system selected by stratified random sampling.And the results were given a statistical analysis.RESULTS & CONCLUSIONS: A total of 281 questionnaires(46.8%) were sent back from sample hospitals and 29(69.0%) from pilot hospitals.37.1% of the hospitals developed the service of mixed dispensing for intravenous drugs,61.7% set up drug information consultation department,32.9% had monitoring software or system for rational drug use,indicating a great evolvement has been achieved in Chinese hospital pharmaceutical services.But there is still great difference in pharmaceutical care between different level and grade of hospitals.

OBJECTIVE: To probe into the status quo of the practice of clinical pharmacy in hospitals of China.METHODS: A mailed questionnaire survey was conducted among 600 sample hospitals and 42 clinical pharmacist work system pilot hospitals based on stratified sampling regarding the status quo of the clinical pharmacy practice in China.And the results were subjected to statistical analysis.RESULTS & CONCLUSIONS: A total of 310 questionnaires were sent back,which revealed that the proportions of developing clinical pharmaceutical services including prescription evaluation,ADR monitoring,drug information consultation in hospitals at different level were high,but the proportions of developing such pharmaceutical services as medication orders' writing and drug-food interaction monitoring were low.The pilot hospitals had better clinical pharmacy services as compared with common hospitals,much as in drug therapy monitoring and individualized medication therapy.The clinical pharmacists were characterized by low quality and scanty numbers.The financial support to clinical pharmacy and the role of the clinical pharmacists remain to be intensified.

Objective　To investigate the direct antitumor effect of several surface molecules of Bifidobacterium bifidum 1101 such as whole peptidoglycan (WPG), lipoteichoic acid (LTA), and polysaccharides (PS) on human colonic adenocarcinoma cell line LoVo. Methods　MTT assay and flow cytometry (FCM) were used to evaluate the effect of Bifidobacterial surface molecules on the growth of LoVo cells. Morphological study, fluorescent polarimetry and differentiation-specific enzyme analysis were used to determine whether LTA treatment could induce the differentiation of the cells. Results　LTA but not WPG or PS significantly inhibited the proliferation of LoVo cells in a dose and time dependent manner. Cell cycle analysis by FCM revealed that LTA induced changes in cycle distribution resulting in G0/G1 phase commitment. Morphological alterations observed by light and electron microscopy indicated maturity of cytoplasm and nucleus following treatment by 50μg/ml of LTA. Membrane fluidity was also altered, and intestinal alkaline phosphatase specific for the differentiation of LoVo cells roused dramatically during culture in LTA. It was further found that LTA elevated ［Ca2+］i which resulted from Ca2+ influx instead of the release of stored calcium. Conclusion　These observations indicated that LTA was able to inhibit the growth of LoVo cells and induce the differentiation of the cells. Calcium influx may play an important role in this process.

Objective To explore the present state of clinical decision-making ability of nursing students in Sichuan Province. Methods The instrument for measuring the clinical decision-making ability of nursing students was adopted te investigate baccalaureate nursing students from four colleges and uni-versifies in Sichuan Province who had completed 10-12-month clinical practice. Results The overall mean score of clinical decision-making ability of baccalaureate nursing students in Sichuan Province was (72.86± 4.53). Among the dimensions, the scoring rate of Goal-setting Ability Dimension was the highest and that of evaluation-feedback ability dimension the lowest. In terms of the qualification rate of clinical decision-making ability, 99.52% of the students were qualified (4.82% good and 94.7% passed) and 0.48% was unqualified. Conclusions Baccalaureate nursing students in Sichuan Province have not showed a high level in clinical nursing decision-making ability and most of them are just qualified. No students have reached the level of excellence.

Based on the ICF framework and functional vision theories, some elements of an evaluation system for visually impaired children were extracted, including body structure and function, activities and participation, and environmental factors.

Objective: To compare the ocular parameters of acute angle closure glaucoma (AACG) and chronic angle closure glaucoma (CACG). Methods: Totally 106 patients with primary angle closure glaucoma were recruited: 58 patients with AACG and 48 with CACG. All patients were divided into 3 groups: AACG attack eyes group, AACG uninvolved fellow eyes group and CACG group and underwent the same ophthalmic examinations, comprising optometry, keratometry, and A-scan ultrasonography. The lens/axial length factor (LAF) and relative lens position (RLP) were calculated. Results: The AACG attack eyes had a significant shallow anterior chamber depth, thick lens, short axial length and larger LAF. hTere tended to be a reduction in the percentage of LAF>0.20 in AACG attack eyes, CACG eyes and AACG uninvolved fellow eyes, though there were no statistically signiifcant difference in all groups (P>0.05). Conclusion: The eyes with AACG attack have a more crowded anterior chamber structure compared with uninvolved fellow eyes and eyes with CACG.