The 2nd derivative spectrophotometry was developed fo r the content determination of gastrodine in Naomaitong Oral Liquid. This methodis simple and rapid. Its' teproducibility is well.

Objective To discuss nursing of patients with esophageal cancer surgery by early feeding of homemade non-factor enteral nutrition.Methods 70 patients of esophageal cancer surgery were accepted homemade non-factor enteral nutrition.Results We got better results in nitrogen balance recovery and the enteral function in intestinal nutrition group than those in apenteric nutrition group after the operation.Conclusion The nutritional status of patients had been markedly improved by application of homemade non-factor feeding after carefully nursing of nasal feeding tube and other tubes,reasonable preparation,maintaining the pipeline opening,observation of illness,oral care,complications of observation and caring etc.The complications have been reduced the quality of life of the patients wag significantly improvad.

Objective To investigate the effects of alprostadil injection on IL-6, IL-8, TNF-αand insulin resistance in diabetic patients.Methods 90 patients with diabetes mellitus in our hospital from January 2014 to May 2016 were selected,and randomly divided into observation group and control group,45 cases in each groups.The control group received conventional western medicine , the observation group was treated with alprostadil injection.The levels of serum IL-6, IL-8, TNF-α, insulin and insulin resistance index were compared before and after treatment in both two groups. Results After treatment,the total effective rate of treatment group was 91.11%,which was significantly higher than the control group 71.11%( P<0.05).Before treatment,the levels of IL-6,IL-8,TNF-α,insulin and insulin resistance index of two groups were had no statistical significance,after treatment,the levels of IL-6,IL-8,TNF-α,insulin and insulin resistance index were decreased in both groups,and the observation group was significantly lower than the control group,the difference was statistically significant (P<0.05).Conclusion The alprostadil injection can significantly reduce the levels of IL-6,IL-8,TNF-α,and control the level of blood glucose,reduce the effect of insulin resistance.

Objective To establish inductively coupled plasma mass spectrometry(ICP-MS) method and determine the migration contents of twelve elements in clevidipine injectable emulsion direct contact with packaging materials.Methods Clevidipine injectable emulsion were digested by carbonization-oxidation.Based on internal standard method, migration contents of twelve elements in digested sample solutions were analyzed by ICP-MS.Results The linear correlative coefficients for twelve elements were higher than 0.9995.The accuracy, precision and stability of the method were detected, all of which conformed to the analysis standard.The recoveries were in the range of 81.3%~119.0%.Conclusion The packaging materials have good compatibility with injectable emulsion, and migration contents of Na、K、Sb and Ba of twelve elements meet the requirements.

Objective To investigate the reversal effect of triazole antifungal itraconazole,fluconazole combined with adriamycin on multidrug resistance in leukemia cells.Methods Human chronic myelogenous leukemia adriamycin resistant cell lines K562/ADR cells were incubated with itraconazole,fluconazole,or PSC833 (positive control) combined respectively with adriamycin.CCK-8 assay was used to assess cell proliferation of K562/ADR.The mean fluorescence intensity of intracellular ADR was measured by flow cytometry.The marker of DNA damage γH2AX was detected by Western blot.Results 1 μg/ml itraconazole and 0.5 μg/ml PSC833 can decrease K562/ADR IC50 of adriamycin from 38.30 μg/ml to 8.59 μg/ml and 24.64 μg/ml in a dose-dependent manner.K562/ADR cells were incubated with 1 μg/ml itraconazole or 0.5 μg/ml PSC833 combined respectively with adriamycin for 3 h and 6 h,the mean fluorescence intensity of intracellular ADR were increased 1.54-fold (3 h),1.50-fold (6 h) or 5.97-fold (3 h),5.83-fold (6 h).Itraconazole or PSC833 combined with adriamycin significantly increase the expression of γH2AX in K562/ ADR cells.Conclusion Itraconazole can recover adriamycin sensitivity of K562/ADR by increasing the concentration of intracelullar adriamycin and synergistically increasing DNA damage,but not for fluconazole.

Objective To evaluate the feasibility and safety of peroral direct cholangioscopy (PDCS) by freehand intubation using routine straight-view endoscope for diagnosis and therapy of biliary diseases.Methods Patients with bile duct dilation underwent standard ERCP and native papilla was dilated using EST and/or endoscopic papillary large balloon dilation,according to the size of the papilla.Then peroral direct cholangioscopy was performed by freehand intubation using routine straight-view endoscope.General data of patients,procedure of ERCP,diagnosis of PDCS and complications were recorded.Results A total of 5 patients underwent PDCS.Indications included common bile duct stones (n =2),intraductal papillary neoplasm of bile buct (n =2) and benign biliary stricture after removable metal stent placement (n =1).The diameter of common bile duct ranges from 1.2 cm to 2.4 cm.EST or/and EPLBD was required in 3/5 patients to facilitate PDCS.Freehand PDCS intubation of the bile duct was successful in all procedures (1 case into distal common bile duct,4 cases into proximal common bile duct).Complications were cholangitis managed with intavenous antibiotics (n =1).No procedure related death occurred.Conclusion Freehand PDCS is technically feasible and safe,and may be clinically useful for diagnostic and therapeutic purposes in managing select biliary tract diseases.