Objective To study the effects of the compound xueshuantong capsule ( FXC) against cerebral ischemia and hypoxia in mice. Methods KM mice were randomly divided into the sham operation group ( normal control group) , model control group, positive control group and FXC at high-,medium-and low-dose(0. 75,1. 50,and 2. 25 g·kg-1 ) groups. After intragastric administration for 7consecutive days,cerebral ischemia model was established by unilateral carotid arteries ligation. The cerebral water content and stroke index were recorded. The viability of the mice was determined by testing atmospheric hypoxia,sodium nitrite poisoning and acute cerebral anoxia. Results FXC decreased water content and stroke index of the mice suffered from unilateral carotid artery ligation dose-dependently (P<0. 05,P<0. 01) . It also prolonged the survival of mice by tolerating absence of oxygen,sodium nitrite poisoning and acute cerebral anoxia (P<0. 05,P<0. 01). Conclusion FXC has a protective effect on cerebral ischemia and hypoxia in mice.

Objective To investigate the effects of morroniside on the expression of vascular endothelial growth factor (VEGF) and fi-broblast growth factor-2 (FGF-2) in rat cortex after focal cerebral ischemia-reperfusion. Methods 30 male Sprague-Dawley rats were ran-domly divided into sham group, model group, morroniside-low group (30 mg/kg), morroniside-middle group (90 mg/kg) and morroni-side-high group (270 mg/kg). Middle cerebral arteries of rats were occluded for 30 minutes with Longa's method and re-perfused. The ex-pression of VEGF and FGF-2 in the ischemic ipsilateral cortex was detected with Western blotting 7 days after reperfusion. Results The ex-pression of both VEGF and FGF-2 increased in the ischemic ipsilateral cortexin in all the ischemic groups compared with the sham group (P<0.05). The expression of VEGF further increased in a dose-dependent manner in all the morroniside groups compared with that of model group (P<0.05), and the expression of FGF-2 increased in the morroniside-high group (P<0.001). Conclusion Morroniside could increase the expression of VEGF and FGF-2 after ischemia-reperfusion, which might promote angiogenesis.

Objective To explore the effects of morroniside on the expression of CD34 in ipsilateral cortex of rats after focal cerebral isch-emia-reperfusion. Methods 45 male Sprague-Dawley rats were divided into sham group (n=9), ischemia group (n=9), and morroniside groups (low, medium and high dosage groups, n=9). The middle cerebral artery were occluded for 30 minutes, and reperfused. Morroniside was administered intragastrically once a day at dose of 30 mg/kg, 90 mg/kg, 270 mg/kg after operation. The expression of CD34 in the isch-emic ipsilateral cortex were detected with immunohistochemistry (n=6) and Western blotting (n=3) 7 days after operation. Results The ex-pression of CD34 increased in the ischemia group compared with the sham group, and further increased in the morroniside groups of high dos-age compared with the ischemia group (F>14.865, P<0.001). Conclusion Morroniside could increase the expression of CD34 in the ischemic ipsilateral cortex after ischemia-reperfusion in rats, which may promote the angiogenesis and neurogenesis after ischemia.