Objective:To establish an HPLC method for the determination of gastrodin in Fufang Zhenma capsules. Methods:A YMC-Pack Pro C18(250 mm ×4.6 mm,5μm)column was used. The mobile phase consisted of acetonitrile-water (2 ∶98). The detec-tion wavelength was 220 nm. The flow rate was 1. 0 ml·min-1 ,the column temperatime was 20℃. the sample size was 25μl. Results:Gastrodin showed a good linear relationship within the range of 3.38-432.80 μg·ml-1(r =0.999 9). The average recovery was 97. 14% (RSD=2. 67%)(n=6). Conclusion:The method is feasible with good specificity and reproducibility, and can be used for the determination of gastrodin in Fufang Zhenma capsules.

Three active compounds were isolated from the marine bryozoan Bugula neritina living in the South China Sea by bioassay-guiding isolation method with a combination of extraction with suitable solvent and multiple column chromatography(Sephadex LH-20, ODS and preparative HPLC). Their structures were assigned as known bryostatin 4, bryostatin 5 and bryostatin 6 by intensive analysis of the data of high resolution 2DNMR(600 MHz,DQF-COSY,TOCSY,HMQC and ROESY). All these compounds were obtained from this bryozoan in the South China Sea for the first time and showed significant antineoplastic activities in vitro.

OBJECTIVE:To establish a method for the quality control of Huangmai granules and provide reference for the qual-ity control. METHODS:TLC was conducted to identify the Leguminosae in Huangmai granules and HPLC was used to determine the contents of icariin,hyperin and stilbene glycoside in Huangmai granules. RESULTS:The spots on TLC plates were clear with good reproducibility. The results of HPLC showed that the linear range was 9.969-319.0 μg/ml(r=0.999 9) for icariin , 12.3-196.8 μg/ml(r=0.999 9)for hyperin and 12.64-202.2 μg/ml(r=0.999 8)for stilbene glycoside;RSDs of precision,stability and reproducibility tests were all no more than 2.92%;the average recoveries were respectively 95.44%(RSD=1.46%,n=6), 101.06%(RSD=1.90%,n=6)and 100.51%(RSD=1.73%,n=6). CONCLUSIONS:The method is simple,reproducible,accu-rate and reliable,and can be used for the quality control of Huangmai granules.

Two active compounds were isolated from chinese marine mollusc sea hare (Notarchus leachii cirrosus) by bioassay guided method with conidia of Pyricularia oryzae , and by a combination of extraction and purification with suitable solvent as well as multiple column chromatographies. Their structures were elucidated as (7 E) 1 acetoxy 8 chloro 7 (dichloromethyl) 3 methyoct 7 en 4 one (Ⅰ), (7 Z) 1 acetoxy 8 chloro 7 (dichloromethy l) 3 methyoct 7 en 4 one (Ⅱ). All compounds were obtained for the first time from this specimen and showed significant antineoplastic activities in vitro .

Eight active compounds were isolated from Bugula neritina Linnaeus collected off Dapen Bay in Guandong province, guided by P 388 bioassay system by a combination of successive chromatographies and preparative HPLC. By intensive analysis of the data of high resolution 2D NMR spectrum and ESI MS, their structures were elucidated as known bryostatins--bryostatin 4, 5, 6, 8, 10, 11, 16 and 18, among which bryostatin 8 and 16 were for the first time obtained from this animal in South China Sea and they showed significant antineoplastic activities in vitro.

Five compounds were isolated from marine sponge Aplysinopsis sp.collected from the South China Sea.Their structures were elucidated by ~1H-NMR,~(13)C-NMR and MS as (E)-3'-deimino-3'-oxoaplysinopsin(Ⅰ),(Z)-3'-deimino-3'-oxoaplysinopsin(Ⅱ),3-(2- oxopropyl )- 3-hydroxyind-olin-2-one(Ⅲ),1H-indole-3-carboxaldehyde(Ⅳ),5?,6?-epoxystigmasta -7-en-3?-ol(Ⅴ).CompoundsⅢ,Ⅴwere isolated from Aplysinopsis sp.for the first time.

Objective:To screen for the bioactive marine organisms and to study the active constituents from them.Methods:Bioactivities of marine organisms extracts were screened by Pyricularia oryzae bioassay method and the constituents were isolated and identified by a combination of multi chromatography and spectral analysis.Results:Twenty ethanolic extracts and 5 aqueous extracts of the tested marine organisms showed activities causing morphological abnormality of P.oryzae mycelia.Fifty six compounds were obtained and identified from Sargassum carpophyllum,Ishige okamurai and Natarchus leachii freeri ,among which 28 compounds showed activities against P.oryzae and exhibited cytotoxicities on various cultured tumor cell lines,eight compounds showed inhibition effects on Fonsecaea pedrosoi in vitro .Conclusion:The screening data and bioactivity evaluation suggest the P.oryzae bioassay is suitable for preliminary screening of bioactive agents from marine organisms.

Objective: To analyze the uncertainty of quercetin concentration determination in Sicao Tongmai capsules by HPLC. Methods: The source of uncertainty was confirmed by analyzing the HPLC determination process. The uncertainty components were quantified by statistics, and the extended uncertainty and confidence level were finally obtained. Results: The extended uncertainty of the measurement results was 0. 19 μg · g-1 . Quercetin concentration in Sicao Tongmai capsules was (353. 65 ± 0. 19)μg·g-1 . Conclusion: The uncertainty analysis method is suitable for the standard limit formulation for Sicao Tongmai capsules, and it is important to establish uncertainty analysis methods for traditional Chinese medicines.

OBJECTIVE:To demonstrate challenges of pharmacy automation reconstruction so as to set solutions. METHODS:Based on literature review,analysis of pharmacy automation setting and features,this paper gave the suggestions and solutions on construction cost,management model change,equipment maintenance and emergency response,etc. according to the practice of the hospital. RESULTS&CONCLUSIONS:Pharmacy automation construction should be stick to the requirements of new health re-form to lower the cost by using the out resources and interior optimal allocation,to improve efficiency by unified planning and proper design,and to ensure the system running efficiently by sufficient maintenance and contingency plan.