Proteomics is an emerging discipline which studies the composition of proteins and their transformation rules at the cellular level and the overall level.Recently,proteomics has been used in a variety of studies of tumors.And it has made some progresses in explaining the mechanisms of tumors' genesis and development,discovering new specific markers for tumors and new targets for drug treatment,and forecasting tumors' biological behaviors.

OBJECTIVE:To determine the formula of alfuzosin rapid-release and sustained-release bilayer tablet and its preparation METHODS:The compositions of rapid-release layer were selected by orthogonal design and the compositions of sustained-release layer were determined according to its percentage of dissolution RESULTS:The rapid-release layer contained alfuzosin 1mg,lactose 35mg,sodium carboxylmethyl starch 1mg and starch 8mg The sustained-release layer contained alfuzosin 4mg,HPMC 50mg and MC 70mg CONCLUSION:The formula of the tablet is reasonable and the preparation simple

Objective To produce Beidougen dispersible tablets.Methods To optimize formula and technology of Bei- dougen dispersible tablets under the control of multiple quality indexes.Results The formula was as follows:CMS- Na,PVPP and MCC.The granules were prepared with 24 mesh riddles,and disintegration time was 70 seconds.The stability was better.Conclusion The formula is reasonable.

BACKGROUND:Calcar plays an important role in hip arthroplasty.The reconstruction of calcar includes bone cement reconstruction and restoration of fracture fragments,but there is some lack of knowledge about their comparative study.OBJECTIVE:To analyze the biomechanical characteristics of remodeled calcar using bone cement reconstruction and restoration of fracture fragments in senile intertrochanteric fractures through three-dimensional finite element models.METHODS:The remodeled calcar using restoration of fracture fragments (model A) or bone cement reconstruction (model B) in senile intertrochanteric fractures was obtained.The stress distribution on the bone interface and prosthesis,the overall stress and displacement of the two models were observed.RESULTS AND CONCLUSION:(1) The maximal strain and stress on the bone interface and prosthesis of model A all were more than those of model B.The mean stresses at each node on the bone interface and prosthesis of model A all were significantly higher than those of model B (P ＜ 0.05).(2) The maximum stress and maximum displacement values of the model A all were higher than those of model B.(3) These results show that the maximal stress and displacement values of the remodeled calcar using restoration of fracture fragments are higher than those of bone cement reconstruction in senile intertrochanteric fractures;the latter holds even stress distribution and better repair outcomes.

Objective To analysis on the 25-hydroxyvitamin D[25-(OH)D] level of preschoolers in Yangzhou ,evaluating their nutrition conditions ,to discuss the influence factors .Methods The 25-(OH) D in plasma of peripheral vein was measured in the preschoolers who came to Maternal and Child Care Service Hospital of Yangzhou receiving healthy examinations in 2014 all over the year ,SPSS17 .0 software was used to analyze the data .Results The vitamin D level of preschoolers had statistical significance be-tween different age group .Season and vitamin D level of pregnant women also contributed to the statistical significance .Conclusion The vitamin D level of preschooler children shows low ,the supplement of vitamin D of pregnant women could improve the new -borns ,reduce the rate of illness .

Peroxisome proliferator-activated receptors (PPARs) belong to the nuclear hormone receptor superfamily of ligand-dependent transcription factors.To date,three isotypes of PPARs called PPARα,PPARγ and PPARβ/δ have been identified.The relationship between PPARγ and fibrosis disease has increasingly drawn people's attention.PPARγ is activated by its ligands or agonists plays an important role in the control of fibrosis through their effects on various cellular processes,including anti-inflammatory,inducing cellular apoptosis and differentiation,anti angiogenesis,antioxidative and anti-proliferative effects,which is expected to be a new way to treat ocular diseases.The role of PPAR-γ and its agonist in ocular diseases,including PPARs and its distribution,the structure and function of PPAR-γ,PPAR-γ ligands and agonist,activation of PPAR-γ,PPAR-γ in the expression of eye tissues,and the role of PPAR-γ and its agonist in ocular diseases were reviewed.

In a variety of pathological conditions,capillaries grow from corneal limbus to the central cornea area,to form corneal neovascularization (CNV).CNV destroys the normal corneal microenvironment, reduces the transparency of cornea, seriously affects visual acuity and leads to corneal transplantation immune rejection.Corneal microenvironment includes inflammatory mediators, cytokines and extracellular matrix (ECM).Studies showed that corneal microenvironment changes have a close relationship with CNV.This article reviewed the relationship of corneal microenvironment with CNV.

Neovascular glaucoma (NVG) is a kind of devastating secondary glaucoma characterized by neovascularization in iris and anterior chamber angle,which often results in loss of vision.The current standard of treatment includes retinal ablation and control of enhanced intraocular pressure with drugs and surgical therapy.There are a lot of surgical therapies of NVG,while the prognosis of these therapies are not satisfactory as most of them would be effected by different kinds of complications.Ahmed glaucoma valve implantation (AGVI) is one of the main treatments means being widely used with less postoperative complications,such as shallow anterior chamber,low intraocular pressure.Failure of AGVI mainly caused by fiber hyperplasia and neovascularization.AGVI combined with anti-fibrosis therapy and anti-vascular endothelial growth factor (VEGF) therapy might be a promising method to promote surgical outcomes.It has been shown that AGVI combined with anti-VEGF drugs can improve the success rate of surgery.Besides,some researchers found that AGVI with intraoperative application of anti-fibrosis drugs,such as mitomycin C,sustained-release anti-fibrosis drugs can improve the prognosis of surgery in neovascular glaucoma patients.The progress of research on anti-fibrosis and anti-VEGF treatment combined with Ahmed glaucoma valve implantation was discussed in this review.

AIM: To compare the efficacy of vitrectomy combined with internal limiting membrane peeling and C3F8 or sterile air tamponade for idiopathic macular hole (IMH).METHODS: In this research, 51 cases of IMH with holes diameter ≤600μm were accepted from the same doctor of department of Ophthalmology in Luoyang Central Hospital from January 2011 to January 2016.All the patients were under taken vitrectomy combined with internal limiting membrane peeling and gas tamponade.All the study subjects were divided into two groups (Group A and Group B).The Group A were tamponaded with perfluoropropane(C3F8)(27 eyes), while the Group B were tamponaded with sterile air (24 eyes).The closure rate and morphology of macular hole, the time of prone position, the best corrected visual acuity(BCVA), the improvement of metamorphopsia and the process of cataract were compared and statically analyzed in these two groups.While the closure rate was compared among ≤200μm, >200-400μm and >400-600μm groups.Postoperative followup time was 1wk, 1, 3mo and half a year.RESULTS: The difference of two groups was no statistically significant in all interval according to holes diameter (P>0.05).The difference of two groups was no statistically significant in morphology of macular hole form(P>0.05).The time of prone position in Group B was significantly shorter than that in Group A, and the difference was statistically significant(P<0.01).The BCVA of postoperative was improved than preoperative, and the difference was statistically significant(P<0.01).However, there was not statistically significant between the two groups in the BCVA of postoperative(P>0.05).The metamorphopsia of postoperative was improved than preoperative in two groups while there was not statistically significant between the two groups in the BCVA of postoperative(P>0.05).Cataract progress rate was reduced in the Group B than the Group A, but the difference was not statistically significant(P>0.05).CONCLUSION: This study shows that sterile air tamponade can obtain same closure rate, morphology of macular hole and the improvement of metamorphopsia and visual function in holes diameter ≤600μm when reducing the time of prone position and the risk of systemic diseases induced by prone position after operation.

Specimens of gastric mucosa were obtained from 16 cases of fetal stomach, aging from 11 weeks to full term, 5 cases from gastric adenocarcinoma, one case from gastritis and one case from normal adult. The contents of their mucosubstances were compared histochemically with Mowry's alcian blue and PAS techniques. The results are as follows:The mucosubstances of gastric mucosa undergo a conspicions change during the fetal life. In general, acid mucopolysaccharide appear at first in the epithelium and rudimentary gastric pits and it is replaced subsequently by neutral mucin. This mucosaccharide replacement varies within different areas and stages. The neutral mucin always takes its appearance about the 11th week at the cardiac and pyloric regions where the mucosubstance have begun to reveal pruple colour in AB-PAS reaction. After the 4th month, the neutral mucosacchaide has completed its replacement in both areas except the lower parts of pyloric glands which still show some mixed mucins. In the corpus tomach, acid mucin is present almost during the whole fetal life but converts into mixed type only at birth. The parietal cell do not contain any mucous material during its histogenesis, but the chief cell may contain some alcianophilic granules which will change into faintly PAS-positive at birth. With alcian blue at low pH, it proves that most of the acid mucosaccarides are sulfated mucosubstance.Adjacent to the bottoms of gastric pits and the necks of gastric glands, some acid mucin often remains in 1he epithelial and neck mucous cells of normal adult just as those of newborn. The acid mucosubstance occurs in various degree within the spaces or lumen of adenocarcinoma tissue. It could be considered that the bottoms of pits and necks of glands would be a cellular bases for further development of gastric carcinnoma under certain conditions.