Objective To compare the effects of Niaoduqing Tablet and Niaoduqing Granule in tre atingchronic renal failure(CRF)induced by adenine in rats,which is expected to provide some lab oratory data for the clinical applic ation and innovation of the dosage forms.Methods CRF rat models induced by adenine were administered with Niaoduqing Tabl et or Niaoduqing Granule and then the seru m levels of BUN and Scr,haematologic al and biochemical indexes and renal histolog-ical features were observed.Results Both of two forms reduced serum levels of BUN and Scr,ameliorate the electrolyte,increase the number of red blood cell,hemoglobin content and haematocrit and alleviate pathological changes of the kidney tissue.Conclusion Both Niaoduqing Tablet and Niaoduqi ng Granule is effective in treating CRF in rats and the effect of Niaoduqing Tablet in ameliorating a nemia is superior to that of Niaoduqi ng Granule.

Objective To establish a reasonable method to evaluate nursing quality with both the quantity and quality of nursing taken into account.Methods Quantity indexes of nursing were analyzed with decision tree method (CHAID)in SPSS18.0,which included the number of patients admitted to wards,first grade' s nursing days,critically ill patients,emergencies,operations,intravenous infusions,intramuscular injections,oxygen inhalations,urinary tract catheterizations,gastric tube catheterizations,enemas and the average turnover rate of beds.After the quantity analysis,the nursing units were divided into different groups according to their workload.Then,we made a comprehensive evaluation of nursing quality with 12 indicators on the same group.Results Decision tree analysis can identify hierarchy relationship of various nursing quantity indicators and high-priority work quantity variables.Furthermore,it can establish the hierarchical rule based on the distribution of high-priority variables,and serve the purpose of classifying nursing units based on nursing workload.Conclusion The decision tree method can provide a same-level nursing workload platform for quality examination.This has minimized the influence of nursing workload discrepancies on nursing quality examination,and made nursing quality more comparable with nursing workload units of the same level,thus a scientific method for comprehensive nursing quality evaluation.

Objective To investigate the effects of Yifuning soft gelatin capsules(YSGC)on immune function in ovariectomized (OVX) rats.Methods Fifty female mature Sprague-Dawley rats were randomized into 5 groups:normal control, model control, diethylstilbestrol tablets(DT)and YSGC(high-and low-dose). After 4-week treatment,the serum E2 and IL-2 levels were detected by radioimmunoassay. The estrogen receptor(ER)level in spleen were detected with method of radioligand receptor assay(RRA).The pathologic changes of thymus were observed under light microscope and the body weight,thymus index and spleen index were detected too.Results YSGC could obviously increase the serum levels of E2 and IL-2,increase spleen index and ER content in the spleen of OVX rats (P

Objective To investigate the effects of Yifuning soft capsules(YSC)on serum sex hormone level and hy-pothalamic,pituitary and plasma ?-endorphin(?-EP)levels in ovariectomized(OVX)rats.Methods After treat-ment for 4weeks,levels of serum sex hormone and hypothalamic,pituitary and plasma ?-EP were detected by radioim-munoassay.Body weight and uterus in dex were also detected.Results YSC could obviously increase serum e strogen(E 2 )level,uterus index and hypothalami c,pituitary and plasma ?-EP levels in OVX rats(P

To unravel the genetic mechanisms of disease and physiological traits, it requires comprehensive sequencing analysis of large sample size in Chinese populations. Here, we report the primary results of the Chinese Academy of Sciences Precision Medicine Initiative (CASPMI) project launched by the Chinese Academy of Sciences, including the de novo assembly of a northern Han reference genome (NH1.0) and whole genome analyses of 597 healthy people coming from most areas in China. Given the two existing reference genomes for Han Chinese (YH and HX1) were both from the south, we constructed NH1.0, a new reference genome from a northern individual, by combining the sequencing strategies of PacBio, 10× Genomics, and Bionano mapping. Using this integrated approach, we obtained an N50 scaffold size of 46.63 Mb for the NH1.0 genome and performed a comparative genome analysis of NH1.0 with YH and HX1. In order to generate a genomic variation map of Chinese populations, we performed the whole-genome sequencing of 597 participants and identified 24.85 million (M) single nucleotide variants (SNVs), 3.85 M small indels, and 106,382 structural variations. In the association analysis with collected phenotypes, we found that the T allele of rs1549293 in KAT8 significantly correlated with the waist circumference in northern Han males. Moreover, significant genetic diversity in MTHFR, TCN2, FADS1, and FADS2, which associate with circulating folate, vitamin B12, or lipid metabolism, was observed between northerners and southerners. Especially, for the homocysteine-increasing allele of rs1801133 (MTHFR 677T), we hypothesize that there exists a "comfort" zone for a high frequency of 677T between latitudes of 35-45 degree North. Taken together, our results provide a high-quality northern Han reference genome and novel population-specific data sets of genetic variants for use in the personalized and precision medicine.