Objective:To investigate the influence of TGP on IFN-?,TNF-?and IL-10 in prostatic tissue of CAP rats.Methods:40 aged sprague dawley rats were randomly divided into four groups:the control group(10 rats),the rat model for CAP group(10 rats),the rat model for CAP treated with low dose TGP group(10 rats)and the rat model for CAP treated with high dose TGP group(10 rats).The changes of prostate gland inflammation was investigated by HE stain.The expression of IFN-?and TNF-?in rat prostatic tissue was detected by immunohistochemistry(sp).The expression of IL-10 in rat prostatic tissue was detected by enzyme linked immunosorbent assay(ELISA).Results:(1)The changes in histopathology in the animal model were similar to those in clinical CAP and the inflammation of treatment group obvious change for the better;(2)The IFN-?,TNF-?and IL-10 in the rat model for CAP group were higher than the control group;(3)The IFN-?,TNF-?and IL-10 expression reduces along with the TGP dosage increases in the rat model for CAP (P

Objective:To study the role of IFN-?,TNF-? and IL-10 in CAP rats and approach the pathogenesis of CAP.Methods:20 Aged Sprague Dawley rats were randomly divided into two groups: the control group(10 rats) and the rat model for CAP group(10 rats).The changes of prostate gland inflammation was investigated by HE stain.The expression of IFN-? and TNF-? in rat prostatic tissue was detected by immunohistochemistry (sp). The expression of IL-10 in rat prostatic tissue was detected by enzyme linked immunosorbent assay (ELISA). Results:(1) The CAP model group's histopathology express the obvious inflammation performance;(2) The IFN-?,TNF-? and IL-10 in the rat model for CAP group were higher than the control group (P

Objective To study the role of serum C-reactive protein (CRP) ,supplement C3 and CA in sys-temic inflammatory response syndrome (SIRS) induced by acute cerebral infarction(ACI).Methods The levels of CRP,C3 and CA were measured by automatic immunoturbidimetry in 52 ACI cases accompanied with SIRS (SIRS group) ,50 cases of acute simple cerebral infarction(SACI group) and 46 normal cases (control group).Results The serum CRP, C3 ,and CA levels were significantly different among the three groups( F=720.6,580.8,94.1 ,P <0.01) ,the serum CRP levels in SIRS group were significantly higher than those in SACI group(P <0.01 ).The ser-um C3 and CA levels in SIRS were significantly lower than those in SACI group(P <0.01).With the diagnosis crite-rion items increasing, the serum CRP levels tended to be higher (t = 7.69 ,P < 0.01 ), and the serum C3, CA levels tended to be lower meeting SIRS diagnosis criterion numbers increasing respectively (t=10.68 and 10.53, P <0.01).Conclusion The serum ClIP levels are higher significantly and the serum levels of C3 and C4 are lower sig-nificantly when ACI progresses to SIRS.The serum CRP,C3,CA level changes can be used as an index for judging the progress of ACI progressed to SIRS.

Objective To explore the mechanism of the acute cerebral infarction leading to systemic inflammatory response syndrome (SIRS) which causes multiple organ dysfunction syndrome (MODS), and to explore the role of tumor necrosis factor (TNF-?) and IL-1? in the mechanism from SIRS to MODS.Methods 68 cases of acute cerebral infarction (ACI) were divided into three groups. They were 36 cases of simple acute cerebral infarction (SACI group), 32 cases accompanied with SIRS (SIRS group) and 24 cases accompanied with MODS (MODS group). Enzyme linked immunosorbent assay (ELISA) method was used to measure serum TNF-? and IL-1? contents at different time after ACI and compared with 28 healthy persons.Results (1) 47.06% of the 68 cases were accompanied with SIRS, of whom 75.00% were accompanied with MODS.(2) The serum TNF-? and IL-1? levels were the highest in MODS group, next in SIRS group, then in SACI group, the lowest in control group. The differences were significant among 4 groups (all P

Objective To explore the risk factors of earlier neurological function deterioration in acute cerebral infarction patient.Methods 157 cases with acute cerebral infarction had been studied,including the patient's history,general syndromes,clinical characteristic,CT or MRI and laboratory test.Multivarivate noncondition stepwise Logistic model analysis was made after single variable analysis.Results There were 58 patients with neurological function deterioration in 157 cases acute cerebral infarction patient.The morbidity was 36.3%.The following nine factors were associated with earlier neurological function deterioration in acute cerebral infarction i.e. the history of hypertension and diabetes,CT or MRI abnormal,higher WBC count,fever,higher blood sugar,lover mean arterial press,higher fibrin,higher serum Fe-protein;But age,sex,weight,the history of drink and smoker,blood lipemia lever were associated with the disease.Conclusion Earlier neurological function deterioration in acute cerebral infarction patient is caused by multiple factors.Singer test is not easy to calculate the result.To analyze generally patient's clinical material may calculate if earlier neurological function deterioration in acute cerebral infarction patient takes place.

Objective To explore the relationship between Helicobacter pylori (Hp) infection and psoriasis vulgaris, and investigate the effect of anti-Hp therapy on the patients with psoriasis vulgaris. Methods 13C-urea expiration test and detection of Hp antigen in feces samples were performed to identify Hp infection in 62 psoriasis patients and 35 healthy persons. Detection results of the two groups were statistically analyzed. The 62 psoriasis patients were divided into two groups based on the detection of Hp infection: Hp positive group (32 cases) and Hp negative group (30 cases). Patients in Hp positive group were given anti-Hp triple therapy (omeprazole, amoxicillin, clarithromycin) for two weeks and topical application of tretinoin for six weeks, while the patients in Hp negative group were treated only with topical use of tretinoin for six weeks. The total effective rate, score of psoriasis area, severity index (PASI) and visual analogue scale (VAS) of pruritus were recorded and then analyzed. Results The Hp infection rate in 62 psoriasis patients was 51.6%, and in healthy individuals was 22.9%, the difference between the two groups was statistically significant (P

Objective To explore the dynamic alteration of intracellular free calcium concentration([ Ca2+]i),ATP level and membrane Ca2+-Mg2+ATPase acti vity of erythrocyte in the patients with acute cerebral infarction(CI).Methods we examined [Ca2+]i,ATP level and membrane Ca2+-Mg2+ATPase activity of erythrocyte in 30 patients with acute CI and 28 health controls by Fluorescence Activated Cell Sorter. Results [Ca2+]i in erythrocyte increased significantly in CI group(P<0.01),while the ATP level and membrane Ca2+-Mg 2+ATPase activity were lower than the controls(P<0.05,P<0.0 01).The above result was more remarkable in the elderly group than the young one .The dynamic alteration of [Ca2+]i in erythrocyte increased obv iously during 1～2 days after the attack,and reached the peak in 3～7 day s,it decreased slowly to the slightly low level at the beginning of th e attack in about two weeks,but it was still higher than the controls.The dynamic alteration of ATP level and Ca2+-Mg2+ATPase activit y after acute CI,it decreased significantly during 1～2 days after the at tack,and reached the lowest in 3～4 days.this status could last about one week.Then both of them increased slightly. There was remarkable negative correlation betwe en RBC [Ca2+]i and ATP level or membrance Ca2+-Mg2+ A TPase activity (r=-0.904,r=-0.978,P<0.05).There was positive correl ation between ATP lev el and membrane Ca2+-Mg2+ATPase activity(r=0.835,P<0.05 ).Conclusion There was calcium overload [Ca2+]i in th e intracellular of erythrocyte in acute CI,ATP level and membrane Ca2+ -Mg2+ATPase activity of erythrocyte CI was involved in the pathologi cal course of calcium overload,and related to the age.

Objective The aim of this study was to investigate the effects of different anesthetic drugs and techniques on serum SIL-2R, IL-6 and TNF-? in cancer patients undergoing elective thoracic surgery, trying to find a reasonable anesthesia for patients with compromised immune function. Methods Eighteen ASA Ⅰ-Ⅱ cancer patients(male 12, female 6) undergoing elective thoracic surgery were studied. The age ranged from 46-47 years. 6 patients suffered from lung cancer and 12 from cancer of esophagus or cardia. The patients received no radio-or chemotherapy before surgery. They were premedicated with intramuscular pethidine 50mg and atropine 0.5mg. Esophogus and cardia cancer patients were randomly divided into two groups: group Ⅱ and Ⅱ . Group Ⅲconsisted of only lung cancer patients. Group I received general anesthesia combined with continuous epidural block and postoperative epidural analgesia. An epidural catheter was inserted at T7-8 or T8.9 before general anesthesia. Anesthesia was induced with 2% thiopental sodium 5mg kg-1 and vecuronimn 0.06-0 .08mg kg-1 and maintained with 1%-2% isoflurane inhalation with epidural 2% lidocaine 6-7m1o h-1 . After operation epidural analgesia was produced with 0.125% bupivacaine 5-6ml h-1 for 72 h. In group Ⅱ the induction of general anesthesia was the same as in group Ⅰ , but anesthesia was maintained with ketamine infusion 1-2mg kg-1h-1 and epidural 2% lidocaine 6-7ml h-1. Postoperative analgesia was produced with epidural 0.1% bupivacaine + 0.0003% fentanyl 5-6m1o h-1 for 72 h. In group Ⅲ the induction and maintenance of anesthesia were the same as in group Ⅱ but postoperative analgesia was produced with epidural 0.1 % ketamine 4-5ml h-1. Blood samples were taken from peripheral vein before anesthesia, before and 2 h after skin incision and on the 1st, 3rd and 5th day after operation for determination of serum SIL-2R, IL-6 and TNF-? by ELISA(Genozyme Co) Results The serum SIL-2R, IL-6 and TNF-? levels were comparable before anesthesia and operation among the three groups. Serum SIL-2R decreased significantly at 2 h after skin incision(P

OBJECTIVE:To investigate the effects of total glucosides of paeony(TGP) on the expressions of CD4+ T lymphocytes and IL- 6 in prostatic tissues of rats with chronic abacterial prostatitis(CAP) and to study its action mechanism on CAP.METHODS:36 SD rats were randomly divided into three groups:CAP control group(n = 12 rats),low dose CAP + TGP group(n = 12) and high dose CAP + TGP group(n = 12).The expressions of CD4+ T lympholeukocytes and IL- 6 in prostatic tissues of rats were detected by immunohistochemistry after medication were investigated,and the changes in histopathology of prostatic tissues.RESULTS:Significant inflammatory condition was noted in CAP control group;improvement of glandular structure and infiltration of small number of inflammatory cells were noted for low dose TGP group.In high dose TGP group,inflammation was abated markedly and there was diffused distribution of a few number of inflammatory cells. As compared with CAP group,the expressions of CD4+ T lymphocytes and IL 6 were down regulated significantly in both the high dose and low dose TGP groups(P

60yr, weighting 50-80kg undergoing general thoracic surgery (lung resection and esophagus cancer resection) were enrolled in this study. Their preoperative hepatic and renal function were normal (blood creatinine