Objective:To study the role of IFN-?,TNF-? and IL-10 in CAP rats and approach the pathogenesis of CAP.Methods:20 Aged Sprague Dawley rats were randomly divided into two groups: the control group(10 rats) and the rat model for CAP group(10 rats).The changes of prostate gland inflammation was investigated by HE stain.The expression of IFN-? and TNF-? in rat prostatic tissue was detected by immunohistochemistry (sp). The expression of IL-10 in rat prostatic tissue was detected by enzyme linked immunosorbent assay (ELISA). Results:(1) The CAP model group's histopathology express the obvious inflammation performance;(2) The IFN-?,TNF-? and IL-10 in the rat model for CAP group were higher than the control group (P

Objective:To investigate the influence of TGP on IFN-?,TNF-?and IL-10 in prostatic tissue of CAP rats.Methods:40 aged sprague dawley rats were randomly divided into four groups:the control group(10 rats),the rat model for CAP group(10 rats),the rat model for CAP treated with low dose TGP group(10 rats)and the rat model for CAP treated with high dose TGP group(10 rats).The changes of prostate gland inflammation was investigated by HE stain.The expression of IFN-?and TNF-?in rat prostatic tissue was detected by immunohistochemistry(sp).The expression of IL-10 in rat prostatic tissue was detected by enzyme linked immunosorbent assay(ELISA).Results:(1)The changes in histopathology in the animal model were similar to those in clinical CAP and the inflammation of treatment group obvious change for the better;(2)The IFN-?,TNF-?and IL-10 in the rat model for CAP group were higher than the control group;(3)The IFN-?,TNF-?and IL-10 expression reduces along with the TGP dosage increases in the rat model for CAP (P

With the development of space medicine ,traditional Chinese me dicine a nd Chinese herbs have been widely used in counteracting motion sickness，bone loss，muscle atrophy，and circulation system disorder，improving bod y′s adaptability and protecting the effect of irradiation，hypoxia and noise.This paper summarized the application of basic theories of traditiona l Chinese medicine,Chinese herbs,acupoint treatment and Qigong in space medical research.

Objective To investigate the effects of ACEI combined with beta-blocker on cardiac function in patients with chronic heart failure (CHF)and explore its action mechanisms.Methods 60 CHF patients were enrolled in this study.The patients were randomly divided into the two groups:conventional treatment group(group A,n =30),ACEI combined with beta-blocker treatment group (group B,n =30).The LVEF,LVEDD,MMP-9,and hsCRP were recorded after treatment.The expression of MMP-9 was measured by ELISA.The level of serum hs-CRP was measured by latex enhanced immunoturbidimetric assay.Results The improvement of LVEF and LVEDD in B group [(63.21 ± 6.09) ％,(58.42 ± 5.73)mm] were better than those in A group [(41.13 ± 3.67) ％,(66.24 ± 4.24) mm] (t =7.62,6.54,P ＜ 0.01).The expressions of MMP-9 and hs-CRP in group B [(1.31 ± 0.24) ng/ml,(6.34 ± 0.58) mg/L] were significantly lower than those in A group[(2.23 ±0.39) ng/mL,(8.31 ±0.74) mg/L] (t =6.82,7.03,P ＜0.01).The total effective rate in A group was 36.7％,which was significantly lower than 73.3％ in B group(x2 =6.90,P ＜ 0.05).Conclusion The cardiac function was improved by ACEI combined with beta-blocker treatment in CHF patients.The downregulation of MMP-9 and hs-CRP may play a key role in the effective ACEI combined with beta-blocker treatment.

Objective To explore the independent prognostic value of plasma myeloperoxidase (MPO) in cardiovascular event of patient with stable angina pectoris.Methods Two hundred and forty-seven patients with stable angina pectoris (defined as 50％ stenosis in any coronary vessel) were selected as our subjects and they all were followed up for 2 years.Plasma MPO and hsCRP concentrations were measured.The major adverse cardiovascular events (MACEs),including death,myocardial infarction and stroke,occurred in patients were recorded.Results All patients were divided into high MPO group (84 cases and plasma MPO concentrations over 320 mg/L) and low MPO group (163 cases,plasma MPO concentrations less than 320 mg/L).Compared with patients in low MPO group,the risk of occurring MACEs in high MPO group was higher(HR =1.67,95％ CI =1.32-2.57,P ＜ 0.05).There was positive correlation between plasma MPO and hsCRP concentrations (r =0.132,P ＜ 0.05).Conclusion MPO level may serve as a better prediction index for adverse cardiovascular events of patients with Stable angina pectorisindependently.MPO has relationship with hsCRP concentrations.

Objective:To explore the inhibitive effects of exogenous nitric oxide on the proliferation of in vitro cultured human keloid fibroblasts.Methods:Fibroblasts was isolated from fresh pathological scar tissue and cultured.Fibroblasts were distributed in five groups.Fibroblasts in control group A was cultured without SNP;in experiment group B～D,SNP was used in the concentration of 100、200、300 ?mol/L each;in experiment group E,Fibroblasts was cultured with 200 ?mol/L SNP and 10 ?mol/L methylene blue.After 24h,Griess and MTT were used to detect the concentration of NO and cell vigor.Results:The release of NO increased along with the enhancement of the concentration of SNP.The amount of living cells in experiment groups decreased compared with the controls as shown by MTT tes(tP

Objective To investigate the clinical characteristics and feasibility of laparoscopic repair of giant hiatal hernia. Methods From January 2008 to August 2010, 25 consecutive patients with giant hiatal hernia underwent laparoscopic repair. Crural closure was performed by means of two or three interrupted nonabsorbable sutures plus a tailored PTFE/ePTFE composite mesh. It was patched across the defect and secured to each crura with staples. Laparoscopic fundoplication was performed concomitantly in 16 cases according to the specific conditions of patients. Para-operative clinical parameters were recorded. All patients were routinely followed up. Clinical outcomes were collected and analyzed. Results All laparoscopic surgeries were accomplished successfully. The operating time was 85 -210 minutes (mean, 106 minutes) ,the operative blood loss was 55 - 150 ml( mean, 94 ml) ,the postoperative hospital stay was 4 -21 days( mean, 6.8 days). The symptoms in most cases were adequately relieved after operation. There was no severe postoperative morbidity. After the follow-up period of 3 - 35months ( mean, 13.6 months), the satisfaction rate of surgery was 88%. 4 cases had mild symptom recurrence of acid reflux.Hiatal hernia recurrence occurred in 1 case. Conclusions Laparoscopic repair of giant hiatal hernia is a safe and effective minimally invasive procedure, with the advantages of minimized trauma, quick recovery and reliable effect. The use of a tailored PTFE/ePTFE composite mesh ( Bard CruraSoft Mesh)for giant hiatal hernia proved to be effective in reducing the operation time and technique demands, and the rate of postoperative hernia recurrence, with a very low incidence of mesh-related complications.

Objective To investigate the feasibility and clinical value of laparoscopic surgery in treating patients with gastroesophageal reflux disease. Methods From September 2001 to August 2009, 372 patients with gastroesophageal reflux disease undertwent laparoscopic fundoplication, including 146 cases of Nissen fundoplication, 79 Toupet fundoplication, and 147 anterior 180 degrees partial fundoplication. Para-operative clinical parameters were recorded. All patients were routinely followed up. Clinical outcomes were collected and analyzed. Results Laparoscopic surgery was successfully performed in all patients, and no conversions were required. The operating time was 50 -210 minutes (mean, 85 minutes), the operative blood loss was 40 - 150 ml( mean, 86 ml) ,the postoperative hospital stay was 3 - 21 days( mean, 4.3 days ). The symptoms in most cases were adequately relieved after operation. There were no severe postoperative morbidity and mortality. Endoscopy, radiology, esophageal manometry and 24-hour pH monitoring were repeated 3 months after surgery. After the follow-up period of 3 -63 months ( mean, 27.3 months), the satisfaction rate of operation was 92.57%. 19 cases had mild dysphagia when eating solid food. Symptoms recurrence of acid reflux occurred in 6 cases, which were controlled by antacid medications. Hiatal hernia recurrence occurred in 1 case. Conclusions Laparoscopic operation should be the method of choice to treat the moderate to severe gastroesophageal reflux disease, with the advantages of minimized trauma,quick recovery, safety,feasibility and reliable effect. According to individual condition of patients, appropriate fundoplication procedure should be employed carefully to ensure results of operations, reduce operating difficulties and the rate of postoperative complications.

Benign diseases of gastroesophageal junction include gastroesophageal reflux disease,hiatal hernia and achalasia of the cardia.Surgical intervention is superior in the treatment of moderate to severe cases.With the rapid development of laparoseopic technology,minimally invasive surgical procedures,such as laparoscopic fundoplication,laparoscopic rear of hiatal hernia and laparoscopic cardiamyotomy are widely applied with excellent efficacy.According to our experience and clinical study,laparoscopic surgery,with advantages of minimal trauma,rapid recovery,safety and reliable efficacy,could be the first-line treatment for benign diseases of gastroesophageal junction.

Objective To explore the mechanism of the acute cerebral infarction leading to systemic inflammatory response syndrome (SIRS) which causes multiple organ dysfunction syndrome (MODS), and to explore the role of tumor necrosis factor (TNF-?) and IL-1? in the mechanism from SIRS to MODS.Methods 68 cases of acute cerebral infarction (ACI) were divided into three groups. They were 36 cases of simple acute cerebral infarction (SACI group), 32 cases accompanied with SIRS (SIRS group) and 24 cases accompanied with MODS (MODS group). Enzyme linked immunosorbent assay (ELISA) method was used to measure serum TNF-? and IL-1? contents at different time after ACI and compared with 28 healthy persons.Results (1) 47.06% of the 68 cases were accompanied with SIRS, of whom 75.00% were accompanied with MODS.(2) The serum TNF-? and IL-1? levels were the highest in MODS group, next in SIRS group, then in SACI group, the lowest in control group. The differences were significant among 4 groups (all P