Objective To evaluate the effect of percutaneous coronary intervention(PCI) on myocardial perfusion in coronary heart.Methods Fourty-two patients with coronary heart disease who were confirmed to have coronary artery steonsis by the coronary angiography were selected.They were checked by myocardial contrast echocardiography of intravenous before and after treatment by PCI.Then we determined quantitatively the perfusion of coronary microcirculation based on the extent of myocardial imaging and recorded it images.The myocardial contrast echocardiography result was compared before and after treatment by PCI for evaluating the situation of myocardial perfusion and effect of PCL So we can estimte the patients' prognosis.Results There were 39 eases of 42 patients with PCI that their lesion vascular were all opening and unblocked.Repeated radiography showed that their TIMI blood flow was the third level.The partial cross sectional area of all the capillaries [A =15.46 ± 3.27) min] blood flow velocity [β =(0.75 ±0.16)min/s],and myocardial blood flow volume[A - β =(12.00 ±4.51 ) min2/s] dependent on myocardial segments all increased more significantly than those before the PCI[A =(6.68 ± 1.76) min,β =(0.40 ± 0.12) min/s、A · β =(2.82 ± 1.38 ) min2/s] (t =37.2527.58、30.65,P ＜ 0.05 ).After PCI three months the EF value of follow-up patients was increased mote than that of before PCI (t =13.77,P ＜ 0.01 )but the scoring index of ventricular wall motion was decreased more than that of before PCI ( t =8.75,P ＜ 0.01 ).Correlation analysis showed that after PCI A,β,A · β all had a strong correlation ( every P ＜ 0.01 ).Conclusion PCI could effectively improve the situation of myocardial perfusion in myocardial infarction patients.It could save ischemia myocardial in infarction field and also improve the function of left ventricular if patients implemented PCI operation as soon as possible.

Objective To investigate the effects and the possible mechanisms of berberine in human cervical cancer Hela cell line in vitro. Methods Cell growth rate was determined with MTT assay. TUNEL was used to examine cell apoptos rate. The expression of COX-2 and Bcl-2 proteins were detected by immunocytochemistry method. Results Berberine induced cytotoxicity and apoptosis in human cervical carcinoma Hela cells in time-dependent and dose-dependent manner. Berberine could down-regulated the Bcl-2 protein expression of Hela cells,but had no significant influence in the expression of COX-2 protein. Conclusion Berberine can inhibit Hela cell growth of cervical carcinoma and induce apoptosis in vitro. Down-regulation of the Bcl-2 protein expression may be involved in berberine-induced apoptosis of HeLa cells.

Objective To investigate the action mechanism of microRNA‐152(miRNA‐152) in the cisplatin(DDP) resistance process in non‐small cell lung cancer (NSCLC) .Methods The miRNA‐152 level in NSCLC cell line A549 and its cisplatin‐resistant cell line A549/DDP was detected by the real time quantitative PCR(qRT‐PCR) .miRNA‐152 mimic was transfected for increasing the intracellular miRNA‐152 level in A549/DDP .The MTT assay ,inverted miroscope technique and flow cytometry were adopted to observe the effect of up‐regulating miRNA‐152 on cell proliferation inhibition and apoptosis ,meanwhile ,the level changes of intra‐cellular Bcl‐2 and NF‐κB were also observed by adopting qRT‐PCR and Western blot .Results The low expression of miRNA‐152 and the high expression of Bcl‐2 and NF‐κB were found in A549/DDP cells .Up‐regulation of miRNA‐152 enhanced the inhibitory effect of DDP in A549/DDP cells .Furthermore ,after up‐regulating miRNA‐152 ,the inhibiting rate and apoptosis rate of A549/DDP cells caused by DDP were significantly higher than those in the cells without up‐regulating miRNA‐152 ,the difference was statisti‐cally significant(P<0 .05) .In addition ,miRNA‐152 mimic transfection significantly decreased the expression of Bcl‐2 and NF‐κB in A549/DDP cells .Conclusion Low expression of miRNA‐152 may induce the resistance of NSCLC to DDP ,miRNA‐152 could medi‐ate the sensitivity of NSCLC cells to DDP via regulating Bcl‐2 and NF‐κB levels .

Aim To study cytotoxic effects and mechanisms of unsaturated fatty acid from mao er yan of gansu origin on BEL-7402 strain cells.Methods The essential components of unsaturated fatty acid from mao er yan of gansu origin were determined by gas chromatography-mass spectrometry and cytotoxic effects of un-saturated fatty acid on BEL-7402 strain cells were determined by using flow cytometry,methods of MTT [3-(4,5-dimethyl thiazol-2yl)-2,5-diphenyltetrazolium bromide] and electron microscope scanning after 48 hours of the unsaturated fatty acid from mao er yan of gansu origin treatment at concentrations of 0.2,0.8 and 3.2 mg?L-1,respectively.Results The unsaturated fatty acid from maoer yan of gansu origin had significantly cytotoxic effects on BEL-7402 strain cells and inhibitory rates for proliferation of BEL-7402 strain cells were 34.6%、44.8% and 70.7% at those three concentrations,respectively.50% inhibiting concentration(IC_ 50)was 0.812 mg?L-1.Apoptosis rates were 18.6%,22.7% and 24.2%,respectively,and occurred cell membrane microvilli breakdown and cell rupture,compared to those of the control group.Conclusion The unsaturated fatty acid from mao er yan of gansu origin has significantly cytotoxic effects on BEL-7402 strain cells and their mechanisms may be related to inhibit cell division,destroy the structure of cell membrane and mitochondria.

Objective To investigate the correlation between MRI characteristics and tumor pathologic manifestitions of ovarian tumors.Methods 38 cases of benign ovarian tumor with clinic and MRI data were enrolled in this study.The correlation between the results of postoperative pathology and MRI characteristics was analyzed.Results 38 cases showed as ovarian tumor by MRI was diagnosed as ovarian tumor by pathology.So the accuracy of qualitative diagnosis was 100%;48 lesions were detected by surgery and pathology, of which one case had 3 lesions.The detection rate was 87.5%(42/48).Conclusion MRI is a relatively reliable method for the localization and qualitative diagnosis of ovarian tumor,which provides the guidance for surgery.

Objective To investigate the neuroprotective effects of phycocyanin injection by internal carotid artery in acute cerebral ischemic reperfusion in rats.Methods The model of middle cerebral artery occlusion reperfusion(MCAO/R) was established using the intraluminal filament occlusion with 84 healthy adult male Wistar rats,and treated by phycocyanin injection through internal carotid artery.The nervous function scores,brain water content,cerebral infarction volume and cell apoptosis in brain tissues and the phycocyanin effects on above indexes were measured respectively.Results The rats showed some extent of neurobehavioral function disorders after ischemic reperfusion.In phycocyanin group rats,the nervous function scores,brain water content,cerebral infarction volume and the number of apoptotic cells in cortex,striatum and hippocampus reduced significantly compared to the model group.Conclusion phycocyanin injection through internal carotid artery showed obvious neuroproctective effects in acute cerebral infarction in rats.

AIM:To determine notoginsenoside R_1 and ginsenoside Rg_1, Rb_1 in Zidan Huoxue Tablet by HPLC linear gradient elution (total saponins of Radix et Rhizoma Notoginseng). METHODS: Using HPLC method with Hypersil ODS2 C_ 18 column. A mobile phase consisted of acetonitrile-water 0-20 min (20∶80), 20-21 min(40-20∶60-80), 21-30 min(20∶80) gradient elution. The column temperature was at room temperature. The detection wavelength was at 203 nm. RESULTS: The linear ranges of R1, Rg1, Rb1 were 0.52-2.6 ?g (r= 0.999 9 ), 2.106 -10.53 ?g (r= 0.999 6 ) and 2.946-14.73 ?g (r= 0.999 6 ), respectively. The average recoveryies were 99.3% (RSD=1.1%), 100.0%(RSD=0.5%) and 99.7%(RSD=0.9%), respectively. CONCLUSION: The method is selective and reproducible for determination of notoginsenoside R_1 and ginsenoside Rg_1, Rb_1 in Zidan Huoxue Tablet.

Purpose To evaluate the application value of ERCC2 gene polymorphism ( rs3916840 C/T, rs1799793 G/A and rs238416 G/A) detection in molecular pathological diagnosis of breast cancer. Methods The polymorphisms of ERCC2 ( rs3916840, rs1799793 and rs238416) were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay in 101 patients with breast cancer and in 101 cancer-free controls. Results Analysis of the data showed a 0. 287-fold increased risk of breast cancer due to the deletion of genotype GA at rs238416 (P<0. 001, 95%CI: 0. 153 ~0. 537). However, polymorphisms of rs3916840 and rs1799793 were not associated with breast cancer risk (P>0. 05). Furthermore, Heterozygous genotype of rs3916840 was significantly associated with tumor size (P=0. 049), heterozygous genotype of rs1799793 was significantly associated with PR sta-tus and triple negative breast cancer (P=0. 037). Remarkably, the genotype frequency of GA in p53-positive patients was lower than that in p53-negiative patients (P=0. 026). Conclusions These results indicate that the polymorphism of rs238416 of ERCC2 is sig-nificantly associated with breast cancer risk. Tumor size, PR status, triple negative breast cancer, and p53 protein expression are asso-ciated with polymorphisms of ERCC2 (rs3916840, rs1799793 and rs238416) respectively. ERCC2 gene polymorphism detection is useful for the early diagnosis and prognosis evaluation of breast cancer.

ObjectiveTo observe the effects of bone marrow stromal cells (BMSCs) on vessel endothelial cells proliferation and microvessel formation in vitro.MethodsBMSCs and brain vessel endothelial cells were separated from adult and divided into co-culture group of BMSCs and endothelial cells, medium group of BMSCs, comparison group. Endothelial cells proliferation and microvessel formation were observed. ResultsEndothelial cells were promoted to proliferate and formate the microvessel in medium group and co-culture group. And the effect was prominence in co-culture group.ConclusionBMSCs can promote the proliferation and microvessel formation of endothelial cells.

Objective To observe the expression of IL-6 mRNA and study the mechanism of acupuncture in focal cerebral ischemia.Methods In situ hybridization was used to detect the expression of IL-6 mRNA in the sham operation group, control group and acupuncture group 2 h, 6 h, 12 h, 24 h after reperfusion in the brain of middle cerebral artery occlusion rats, meanwhile and also observe ultrastructure in cortex of ischemic brain tissue 24 h after reperfusion.Results The sham operation group showed a basic expression of IL-6 mRNA in cortex and striate body. The cells expressing IL-6 mRNA were increased 2 h after ischemic reperfusion in cortex and striate body and peaked 24 h after reperfusion in control group and acupuncture group. The cells expressing IL-6 mRNA in cortex of ischemic area were stronger in acupuncture group than in control group at every research time point besides 2 h after ischemic reperfusion. The cells expressing IL-6 mRNA striate body of ischemic area were stronger in acupuncture group than in control group at every research time point. Acupuncture can lighten the degeneration of cell nucleolus and necrosis of cell, resume the integrity of the nucleolus, improve the function of mitochondrial and endoplasmic, and have significant difference with control group. Conclusion Acupuncture can increase the cells expressing of IL-6 mRNA.