Objective To further study the mechanism of maternal fetal immune tolerance Methods Chorioplacental tissues were obtained from different gestation stages of normal pregnancy Immuno histochemistry was used to investigate the expression of Fas Ligand (FasL) on the surfaces of human cytotrophoblasts Highly precise color image measure system for immuno histochemistry was used for quantitative analysis Results FasL were expressed on the surfaces of placental cytotrophoblasts throughout normal pregnancy FasL staining areas on cytotrophoblasts of the first trimester, second trimester and term were (91410?8328) ?m 2, (101 322?11 480) ?m 2 and (97461?10517) ?m 2 respectively; Average brightness FasL staining were 0 227?0 032 5, 0 261? 0 021, 0 145? 0 015; and integral brightness were 21 391? 4 636, 25 993? 6 231, 18 588? 3 897 respectively The differences among the first, second and term pregnancy stages were significant Conclusions Like many immune privileged sites, the maternal specific fas + T cell apoptosis induced by FasL on the maternal fetal interface might be one of the significant mechanisms of maternal fetal immune tolerance The expression of FasL on the surfaces of placental cytotrophoblasts plays an important role both in the maintenance of pregnancy and in the normal development of fetus

Objective To study the immunol pathological mechanism of PIH through the expressron of FasL of placental trophoblasts. Methods Immuno histochemistry were used to detect the expression of FasL of placental trophoblasts on moderate and severe PIH patients, HPIS was used to determine the quantity of FasL. The expression of PIH group was compared with that of the normal control group. Result The scale of FasL expression on PIH group (69.628 ?19.103 ?m 2 ) was significantly lower than that of control group(97.461?10.517 ?m 2), P

Objective To investigate the present situation and reasons of emergency department overcrowding, and put forward effective mitigation strategy. Methods By using input-throughput-outputmodel and choosing the method of fishbone diagram analysis, detaily analysis factors of emergency department overcrowding, then implement three- dimensional intervention in hospital, department, personal. Results Patients who stay>48 h in the emergency department(ED) decreased from 11.9％(15225/127941) to 5.3％(7245/136698); patients who stay > 6 h in the Frist Aid Room of ED decreased from 54.6％(3016/5526)to 17.8％(987/5526), both have statistically significant (χ2=3705.04, χ2=1186.32, P<0.01);before the intervention the National ED Overcrowding Study (NEDOCS) index at 0900, 1300, 2100, 0100 was 234.22 ± 62.31, 253.55 ± 59.26, 303.73 ± 160.24, 187.36 ± 25.73; after the intervention the degree of crowdedness of ED at this four points of time was significantly alleviated, NEDOCS index was 193.09 ± 31.87, 187.09 ± 22.65, 187.36 ± 25.73, 154.03 ± 21.56;there were significant differences between the two groups before and after the intervention (t=2.35-4.32, P<0.05). Conclusions To study and discuss the reasons of emergency department overcrowding, it can effectively relieve the happening of emergency department overcrowding through comprehensive intervention measures such as improve the environment, optimize the process, strengthen emergency medical personnel training and management, improve the service level hospital auxiliary support system.

Objective To screen the microsatellites with low occurrence rate of stutter band and establish the effective bovine STR typing system.Methods The tetranucleotide STR loci in bovine genome were searched with Tandem Repeat Finder software.Primers were designed and used to amplify these candidate loci and the PCR products were separated with electrophoresis.DNA samples from 100 head of unrelated cattle were typed.Results Among these candidate loci,6 bovine tetranucleotide STR loci showed high polymorphism,and their CDP and CPE value were 0.99995 and 0.859591 respectively.Conclusion The 6 bovine tetranucleotide STR loci can be used for bovine identification and parentage testing.

Objective To study the validation of fluorescent multiplex microsatellite amplification technique for use in the caseworks of umbilical blood cell transplantation monitor. Methods Six post-transplant recipients of umbilical cord blood cell transplantation were monitored by analyzing microsatellite DNA loci. DNA samples were amplified using a fluorescent labeling primers multiplex amplification system of 15 microsatellites markers, followed by typing on a DNA automated sequencer. Recipients' or donors' microsatellites DNA profiles were compared before and after transplantation. Results All recipients and donors exhibited different DNA profiles. Without reference samples of pre-transplant or donors, the changes of the 15 microsetellites genotypes of the post-transplant recipients still could be analyzed. The recipient type turned to donor type was observed over time. Conclusion Under the condition of using multiplex amplification of the 15 microsatellites to monitor the umbilical blood cell transplantation, reference sample of pre-transplant or donor did not need to be detected simultaneously.

Objective To investigate the effect of ketamine on the delayed rectifier outward potassium currents (IK) using whole-cell patch clamp technique. Methods Pyramidal neurons were enzymatically isolated from Wistar rat hippocampus. The effect of ketamine on the IK was assessed using whole-cell patch clamp technique. We measured the amplitude of the delayed outward rectifier IK by activating depolarizing pulse from -50 mV to 40 mV. Different concentrations of ketamine were added and potassium currents were measured. Results IK was inhibited by ketamine in a concentration-dependent manner. The five concentrations of ketamine (10, 30, 100, 300, 1000 ?mol/L) reduced peak IK currents by (10 ? 4)% , (19?4)%, (31 ?5)%, (50?7)%, (54?8) % respectively, with a mean IC50 of (100?18)?mol/L and Hill coefficient of 1.33?0.48. The V1/2 of activation curve was shifted from (1.82 ? 0.20) mV to (9.30 ? 1.03) mV (n = 8, P

Objective To explore the in-patients' evaluation and the correlative requirement for the bachelor degree nursing students. Methods Investigated 10 patients by using the self-developed interview outline, and then summarized the key points of these talking. Results The evaluation from patients to bachelor degree nursing students had included 6 aspects: attitude, clinical nursing skills, knowing about the concrete condition of patients, the wide of nursing students' knowledge, the health education for patients and the ability of communication and cooperation. Conclusion Different have their own different emphasis when evaluate the nursing students, but the evaluation from patients were praiseful.

[Objective]To explore the clinical effect of the new stem-less total hip arthroplasty.[Method]Exposed the hip joint through posterior-exterior incision,dislocate the femoral head,clear away sick tissues inside joint,throw into the centric pin by guide of the centric localizer,repaired bone by machine of modeling bone,set the cup and fixed by centric screw and the screw of substantia spongiosa bone on trochanter major and trochanter minor of the femoral.The acetabulum prosthesis according to the traditional total hip arthroplasty were fixed.[Result]Nine cases were followed up for average 20 months,evaluated by Harris Score standard,excellent 5 cases,good 2 cases,fair 1 cases,poor 0 case,the rates of excellent and good is 88.89%.[Conclusion]Because of keeping entire neck and part head of the femur,biomechanics conduction and distribute of the stem-less hip of prosthesis achieves natural biomechanics state of the human body,the treatment effect of short-term is satisfying,the treatment effect of long-term will go on get observation in future.

Objective To evaluate the power of the PowerPlex?6 system for paternity testing. Method 633 cases of paternity testing were studied. After the megaplex STR amplification, the PCR products were genotyped by using the ABI377 DNA Sequencer. Results Among the 879 unrelated individuals, 197 alleles and 739 different genotypes were observed. The cumulated discriminating power was 1x 10-30. And the cumulative chance of exclusion was 0.999999999999987. 95 out of 633 cases were excluded. The RCP of all unexcluded cases were ≥0.9990. Gene mutations were found in 19 cases. Conclusion The Po-werPlex?16 system is powerful and reliable for paternity testing.

Objective To study the genetic polymorphisms of 11 Y chromosome specific STR loci in Guangzhou Han population. Method The DNA extracted from blood samples of unrelated individuals in Han population living in Guangzhou were amplified by PCR. The PCR products were analyzed by using PAGE. Results 3-5 alleles were detected in 11 Y - STR loci respectively in Han population in Guangzhou. The minimum GD value was 0.3037 (DYS434), while the maximum GD value was 0.8455 (DYS390) . Conclusion The 11 Y - specific STR loci are highly polymorphic and are suitable for personal identification and paternity testing.