Side population (SP) cells are highly enriched for stem cell activity and characterized by their ability to efflux the vital dye Hoechst 33342,because they express the ATP binding cassette (ABC)-dependent transporter ABCG2.SP cells can be selected from main population using flow cytometric analysis.Currently SP cells have been isolated from many tissues and organs.SP cells of different origins have some common characteristics.This article introduces the classifications,surface marker,and characteristics of SP cells.

Cancer stem cells(CSCs) are a small proportion of tumor cells with the property of stem cells in the tumor tissue;they are capable of self-renewal,multi-lineage differentiation and serve as the source of tumor cells and tumor tissues.The discovery of multiple myeloma CSCs and the study on its relationship with side population cells and niche provide a new interpretation on the pathogenesis of multiple myeloma.

Objective To establish a method for the determination of eugenol in Xiao’er Fuxie Waifu Gel.Methods RP-HPLC method was developed with Lichrospher-C18 analysis column,using methyl alcohol-water(65:35) as mobile phase.The detection wavelength was 280 nm and flow rate was 1.0 mL/min.Results The linear range of eugenol was from 0.108 to 1.724 ?g(r= 0.999 9).The average recovery was 99.1 %and RSD was 1.4 %.Conclusion The method is convenient and accurate.It can be used for the quality control of Xiao’er Fuxie Waifu Gel.

Adult ; Endoscopy ; Female ; Humans ; Intercellular Signaling Peptides and Proteins ; administration & dosage ; therapeutic use ; Male ; Middle Aged ; Nasal Lavage ; Nose ; surgery ; Treatment Outcome

The flushing pump which is applied to clean operative wound has no temperature controlling function up to now, and doctors have to prepare the flushing fluid that has previously been warmed. The flushing pump system with medical constant temperature designed in our laboratory can absorb flushing fluid at the room temperature, and then eject flushing fluid with the temperature in accordance with the requirements of operations at a controlled constant flow rate. The system combines flow rate control with temperature control functions. The flushing pump system includes flushing part, temperature controlling part, key inputting part, liquid crystal displaying part and exceptional situation monitoring part. The present paper introduces the design method and principle of each part of the system at first, and then gives the debug method of all the system parameters. Finally the paper discusses the performance of the system according to the result of the experiment.
Equipment Design ; Equipment and Supplies ; Temperature

This paper presents the preliminary design of data acquisition system of a portable uroflowmeter. The system uses double-hole cantilever pressure sensor. The signal is transferred to ATmega644PA microprogrammed control unit (MCU), converted by A/D (analog to digital) convertor. Then the further data are processed and get the corresponding relationship of weight-time and two curves of urine flow and urinary flow rate. In the measurement accuracy of the device about urine flow, two factors about the placement and height of the data acquisition are analyzed to show the accuracy of the equipment through the Origin 8.0 data analysis software. The design is characterized by low cost and high speed of data collection, real-time, high accuracy.
Data Collection ; Flowmeters ; Humans ; Monitoring, Ambulatory ; instrumentation ; Software ; Urination ; physiology ; Urodynamics ; physiology

Objective:To explore the relations of psychological stress reactions to workplace violence and so-cial acknowledgement among emergency nurses.Methods:Totally 444 emergency nurses from 8 tertiary hospitals and 1 second A hospital in Beijing were selected.Psychological stressful reactions were measured with the Impact of Event Scale-Revised (IES-R).The social acknowledgment from general society and family to victims was assessed with the Social Acknowledgment Questionnaire (SAQ).Results:Among the 444 samples,96.8% sustained verbal assaults,43.2% underwent physical assaults,32.0% encountered threats,and 4.3% suffered sexual assaults.The results of ANOVA analysis indicated that nurses who suffered 3 types of violence had higher scores of IES-R than the ones who endured one type of violence and 2 types of violence[(22.0 ±16.1)vs.(15.4 ±15.6),(18.1 ± 16.1),Ps <0.01)].Similarly,those nurses who suffered 4 types of violence had higher scores of IES-R than the ones who enduredone type of violence and 2 types of violence [(33.4 ±17.1)vs.(15.4 ±15.6),(18.1 ±16.1), Ps <0.01].Regressive analysis showed that family disapproval,general disapproval,and violence types predicted psychological stress reactions,(β=0.13 -0.40,P <0.05,adjust R2 =0.27).Conclusion:It suggests that violence types,social disapproval and family disapproval may play vital roles in predicting the degree of posttraumatic dis-tress in emergency nurses after suffering from hospital violence.

BACKGROUND:With the development of science and technology and modern aerospace, the effects of mechanobiology in extreme mechanical environment—high acceleration are becoming an issue of concern. Studies have shown that high acceleration has certain effects on the cels. OBJECTIVE:Based on a centrifuge, to design a cel loading centrifuge used for exploration of cel mechanobiology under high acceleration. METHODS:For the cel loading centrifuge, a culture plate or/and culture bottle ful of culture fluid was/were loaded with constant acceleration or variable velocity to explore the experimental feasibility. Besides, a finite element model was built by ANSYS software according to structure and properties of the rotor. The rotor system was calculated under equilibrium and dangerous working conditions, respectively, to analyze the stress and deformation distribution. Moreover, the strength of the main shaft was checked under the dangerous working conditions. Then the analysis results of ANSYS were compared with the results of strength check. RESULTS AND CONCLUSION:Experimental findings showed that the culture plate or/and the culture bottle could be used for (0-40)×g highly constant acceleration or variable acceleration loading. Through the simulation and comparison analysis, we confirmed the reliability of the cel loading centrifuge. This cel loading centrifuge can be used to implement the study of cel mechanobiology under high acceleration in the general biology laboratory. It also provides a basis for wide application of cel loading centrifuge in the future.

Background and purpose:The signal transducers factor and activator in transcription 3(STAT3) is a recently studied gene from a variety of solid tumors such as breast, stomach, and ovarian cancer, in which the increase of abnormal expression and activity are accompanied.The aim of this study was to investigate the effects of eukaryotic vectors that express short hairpin RNA(shRNA) in signal transducers and activators of STAT3 on chemosensitivity of human ovarian cancer SKOV3 cells.Methods:Vectors containing shRNA targeting STAT3(pSTAT3-siRNA) were constructed and transfected into SKOV3 cells.These vectors were then divided into 3 groups:SKOV3, SKOV3NS, and SKOV3siRNA.The mRNA and protein expressions of STAT3 were determined by RT-PCR and Western blot, respectively.The growth inhibition and apoptosis rates of the different group cells under chemotherapeutic agents such as cisplatin(20 ?mol/L) were measured by MTT assay and flow cytometry(FCM).Results:MTT assay growth inhibition rates in the tumor cells of the SKOV3 group, SKOV3NS group and SKOV3siRNA group had inhibition rates of 0.46?0.13, 0.44?0.11 and 0.71?0.12.Compared with the SKOV3, SKOV3NS and SKOV3siRNA group, there was a marked increase of SKOV3siRNA group in inhibition rate of cells.The differences were also statistically significant(P0.05).The SKOV3 group, SKOV3NS group and SKOV3siRNA group's cell apoptosis rates were 18?4, 18?3 and 35?4, respectively.However, the SKOV3 group, SKOV3NS group, and SKOV3siRNA group cell apoptosis were significantly increased and the differences were statistically significant(P0.05).The results for the SKOV3 group, SKOV3NS group and SKOV3siRNA in cell STAT3 mRNA were 0.50?0.08, 0.48?0.07 and 0.31?0.09.With the SKOV3 group, SKOV3NS group, SKOV3siRNA group of cells in STAT3 mRNA, its expression in the lungs were significantly lower and the differences were statistically significant(P0.05).SKOV3 group, SKOV3NS group and SKOV3siRNA group of cells checked the results of STAT3 protein were 0.54?0.09, 0.56?0.08 and 0.32?0.09, respectively.The SKOV3 group, SKOV3NS group, and SKOV3siRNA group in STAT3 protein expression was significantly lower and the differences were statistically significant(P0.05).Conclusion:The STAT3 specific shRNA expression vector could effectively suppress the expression level of STAT3 gene in SKOV3 cells as well as enhance their sensitivity to cisplatin.

OBJECTIVE: To investigate the prospective efficacy of chronic sinusitis lost to short-term follow up after nasal endoscopic surgery and the influential factors. METHOD: Prospective efficacy of 153 chronic sinusitis (CRS) was evaluated . All cases were treated by nasal endoscopic surgery at least 1 year ago but lost to follow up within 3 months after surgery. The clinical data of 153 patients were analyzed, including gender, age, educational level, course of the disease, smoking and drinking history, early surgery history, allergic rhinitis (AR) history, nasal polyps, visual analogue scale (VAS) score, nasal endoscopy score, CT examination score, the endoscopic experience of surgeons, medication of intranasal glucocorticoid and nasal irrigation after surgery. Multifactor logistic regression analysis were used to analyze the data. RESULT: Among 153 patient, the symptoms of 32 cases (20.9%) were completely control, while those of 74 cases (48.4% ) were partially control, and the symptoms of 47 cases (30.7%) were not controlled. The curative effect were better for CRS without nasal polyps, AR or early surgery history, with CT examination score < or = 5, operated by surgeons with experience of endoscopy surgery for more than 5 years. and treated with intranasal glucocorticoid for more than -1 weeks after surgery. CONCLUSION Postsurgery follow-up should be paid more attention to in treatment of CRS. Plans of follow up should be adjusted to patients condition. Treatment of postoperative sinus cavity should be minimized when the lesion is slight and operated properly.
Adult ; Aged ; Chronic Disease ; Endoscopy ; Female ; Follow-Up Studies ; Humans ; Lost to Follow-Up ; Male ; Middle Aged ; Sinusitis ; surgery ; Treatment Outcome