Objective To compare the effect of different perfusion methods on early outcomes of elderly patients with acute myocardial infarction.Methods The clinical data of 110 cases including EKG,echocardiogram and angiography were retrospectively analyzed.Of these patients,45 cases were treated by primary percutaneous coronary intervention (PCI),30 cases by intravenous thrombolysis,and 35 cases by PCI combined with intravenous thrombolysis therapy.The incidence rate of cardiovascular events during hospitalization,no reflow incidence,average hospitalized staying time,left heart function and Killip cardiac function grade at 3 months were compared between the three groups.Results There was no significant difference on incidence rate of cardiovascular events between the three groups (P ＞ 0.05).Compared with PCI group and intravenous thrombolysis group,the combination group had lower no reflow incidence,less hospital time and lower Killip cardiac function grade (P ＜ 0.05).In addition,the parameters of LVEF,CI and SVI of combination group were significantly higher than other groups after 3 months (P ＜ 0.05).Conclusion PCI combined with intravenous thrombolysis was safe and effective in treatment of elderly STEMI patients,which could obviously decrease the no reflow rate,improve cardiac function and the early outcomes.

OBJECTIVE:To establish a mathematical model for the dissolution kinetics of the components of the closed diffusion system of Chinese complex prescription,and to study and analyze the parameters of the extraction kinetics of catalpol,an active component in rehmanniae decoction of Liuwei dihuang decoction. METHDOS:The mathematic modeling method was applied to the extraction process of the traditional Chinese medicine; the mathematic model of the extraction process of the Chinese medicine was established based on the extraction principle and Fick law,and the function expression was derived from the fitting of the mathematic model and analysis of parameters,then the model was applied to the study of the kinetic parameters of the catalpol in rehmanniae decoction of Liuwei dihuang decoction. RESULTS:The analytic solution of the dissolution concentration of components consisting of 3 e in exponential form and the analytical method of parameters were obtained in this study. The optimal grain size of the herbal medicine was 60 meshes.Under this condition,the maximal peak time(tmax)of catalpol was 195.1 min;the peak concentration(Cmax) was 0.012 23 mg?mL-1;the maximal extraction rate(P) was 72.95%,and the loss rate(D) was 7.62%. CONCLUSION:The dissolution of the components of the closed soluble diffusion system of Chinese complex prescription fitted linear kinetic mathematical model,and the parameters can be derived from the expression relation of the dissolution concentration.

Objective To establish the quality standard for Ziyin Mingmu tablets. Methods TLC method was used to identify Moutan Cortex, Corni Fructus, Notopterygii Rhizoma et Radix and Acori Tatarinowii Rhizoma. HPLC method was used to determine the content of Salvianolic acid B. Kromasil ODS column (250 mm×4.6 mm, 5μm) was adopted by using MeOH-acetonitrile-1.67%methanonic acid water solution (24∶10∶66) as the mobile phase. The detection wavelength was at 284 nm, column temperature was at 30℃, and the flow rate was 1.0 mL/min. Results The TLC spots were clear without interference of the negative control. The linear range of Salvianolic acid B was within 0.32-1.92 μg (r=0.999 9). The average recovery was 99.91% (RSD=0.63%). Conclusion The method is specific, reproducible and accurate, and can be used for the quality control of Ziyin Mingmu tablets.

Objective To investigate the impact of different irrigation time on oxygenation in-dex and acid-base balance during percutaneous nephrolithotomy(PCNL).Methods Forty patients un-dergoing selective PCNL were included in this study,were divided into two groups:group S,irrigation time<120 min(n=22);group L,irrigation time≥120 min(n=18).PaO2/FiO2 ,pH and BE were determined before and after irrigation,CVP were recorded before irrigation and every 20 min during irrigation. Results The CVP maximum value were higher in group L than that in group S during irrigation (P <0.05).The PaO2/FiO2 were lower after irrigation than that before irrigation in group L(P<0.05).The pH and BE were lower in group L than that in group S after irrigation(P<0.05).Conclusion Irrigation fluid absorption is observed during PCNL.That can provoke oxygenation function decline and metabolic ac-idosis with irrigation time extend,the operation time properly shortened.

As a cell membrane protein of human body,connexins are closely related to the cell metabolism,proliferation and differentiation,which are highly expressed in lung.Recent studies have shown that connexins play an important role in the lung diseases,such as pulmonary fibrosis,lung injury,lung tumors and so on.This review is to summarize the characteristics of connexins molecules,biological function,the distribution in the lung tissues,as well as the possible regulation mechanisms in the lung diseases.

OBJECTIVE:To study the stability of Yinzhihuang injection(YZH) mixed with 5% glucose solution,10% glucose solution,glucose in normal saline,normal saline and Ringer's solution.METHODS:After YZH mixed with infusion fluid under room temperature,the contents of baicalin were determined with UV-spectrophotometry at different time periods within 24h,the apperance was observed,the pH values of baicalin were detected and identified by TLC.RESULTS:Under room temperature,after YZH mixed with Ringer's solution,the pH value of baicalin was markedly changed and a lot of white particles appeared;after YZH mixed with GNS,no changes of pH value and content of baicalin were found within 24h.When YZH mixed with other 3 kinds of infusion fluid,there were no changes in pH value and content of baicalin within 2h,however,in mixing with NS,the number of insoluble particles produced was beyond the standard.CONCLUSION:YZH should not be mixed with NS or Ringer's solution but it is compatible with 5%GS,10%GS and GNS,however,it should be mixed with them just before use and used up within 2h.

Objective To study the genetic polymorphisms of 11 Y chromosome specific STR loci in Guangzhou Han population. Method The DNA extracted from blood samples of unrelated individuals in Han population living in Guangzhou were amplified by PCR. The PCR products were analyzed by using PAGE. Results 3-5 alleles were detected in 11 Y - STR loci respectively in Han population in Guangzhou. The minimum GD value was 0.3037 (DYS434), while the maximum GD value was 0.8455 (DYS390) . Conclusion The 11 Y - specific STR loci are highly polymorphic and are suitable for personal identification and paternity testing.

Objective To study the relationship between serum level of TNF-? and morphologic changes as well as related factors in 3 and 6 months of diabetic retinopathy rats. Methods This research successfully duplicated a model of insulin dependent diabetes mellitus in rats and carried out morphologic observation of retina of diabetic rat when the disease lasted for 3 and 6 months. The serum level of TNF-? by ELISA together with related factors, such as blood glucose (BG)by oxidase method、glucosylated hemoglobin (GHbAlc) 、serum insulin (Ins) and c-peptide (C-P) by RIA were studied. Results In same duration,the levels of TNF-?、BG、GHbAlC in model groups were significantly higher than therapy groups (P

Objective To evaluate the differences between fresh and cryopreserved human umbilical cord blood as well as the stem cell homing.Methods Fresh or cryopreserved human umbilical cord blood cells were injected into lethally irradiated BALB/C nu+ mice.The mice survival rates,the successful transplantation evidence and the migration phenomenon were observed.Results The survival rates of the fresh group and the cryopreserved group were much higher than the control group,but there was no difference between the former two groups.Evidence of human hematopoiesis were observed by means of PCR or FACS.However,the pattern of migration was different berween fresh and cryopreserved cord blood stem cells. Conclusion Human cord blood contains high hematopoietic potential stem cells to cross xenogenetic barriers and successfully engraft in mice.Cryopreservation can keep the hematopoietic potential of cord blood,but may influence the adhesion molecular of the stem cells and thus influence their homing behavior.

Objective To investigate the clinical application prospect of water exchange colonoscopy.Methods A total of 300 outpatients who underwent unsedated colonoscopy were randomized to the WEC group (n =150)or the AIC group(n =150).The maximum pain score, insertion time, the cecal intubation rate and the polyp detection rate were reported by an assistant during the insertion phase and at the end of colonoscopy.Results The maximum abdominal pain scores were 1.77 ± 1.29 in the WEC group and 2.84 ± 1.55 in the AIC group with significant difference (P =0.000).The intubation time of WEC was longer than that of AIC (11.91 ± 3.58 VS 8.03 ± 3.19 ,P =0.000)with no significant difference.There was no significant difference in the cecal access rate between the two groups(97.3％ VS 94.0％ ,P =0.157).The polyp detection of WEC was higher than that of AIC (16.7％ VS 10.0％, P =0.089).In the post abdominal operation subject of colonoscopy procedure, WEC had higher rate of cecal intubation than AIC ,the maximum abdominal pain score were 2.25 ± 1.63 in the WEC group,lower than 3.48 ± 1.45 in the AIC group.Conclusion Water exchange colonoscopy can alleviate patients' abdominal pain symptoms, but with longer insertion time.There are no significant difference in the cecal access rate and the polyp detection rate between two groups.For difficult colonoscopy, water exchange colonoscopy is helpful to increase the rate of cecal intubation.