Objective To explore the clinical features,related diseases,,etiology of inhalation pneumonia and premonition of lung infection in patients age 80 and over.Methods The 104 patients over 80 years old with pulmonary infection were analyzed retrospectively,statistical analysis of the main clinical manifestations,associated diseases and pathogens.Results Aspiration pneumonia (AP)in 77 cases (74%),Chronic respiratory disease accounted for only 26%,mostly associated with serious cardiovascular and cerebrovascular diseases,cognitive disorder,Chronic gastrointestinal diseases(Gastritis,reflux esophagitis,gastrointestinal dysfunction,constipation),long-term bed,Can not take care of themselves.Sputum bacterial cultures isolated from 66 (63.5%)strains,Gram positive cocci were 18 strains (27.3%),Gram negative bacilli were 38 strains (57.6%),Fungal strains were 15 strains (22.7%).Conclusion In the elderly over the age of 80,pulmonary infection mostly occured by Aspiration pneumonia.We should strengthen the awareness of Aspiration pneumonia,especially accompanied by cough,chronic gastric disease.Take reasonable application of antibiotics according to the pathogen test results.

[Objective] To observe the effect of acupoint sticking at“Zusanli”(ST36) with magnolia powder on patients with acute exacerbations of chronic obstructive pulmonary disease and gastrointestinal dysfunction. [Method] A total of 78 patients with acute exacerbations of chronic obstructive pulmonary disease(AECOPD) and gastrointestinal dysfunction were randomly divided into two groups: treatment group(n=40) and control group(n=38). Al patients received conventional therapy for AECOPD and tablet mosapride 5mg tid for 3 days. Patients in treatment group also received acupoint sticking at“Zu-sanli”(ST36) with magnolia powder q8h for 3 days. [Result]The total effective rate was 75.0%for treatment group,and 44.7%for control group. The total effective rate of treatment group was higher than that of control group, and no marked side effects were found. [Conclusion] Acupoint sticking at“Zusan-li”(ST36) with magnolia powder combined with mosapride can relieve gastrointestinal dysfunction of patients with AECOPD.

The purpose of this study was to observe whether HBV can infect HSC in vitro. LX-2 cells, the human activated HSC cell line, were incubated with human serum containing HBVDNA at final concen-trations from 0.01 to 10 copies/cell and harvested after 24,48 and 72 hours. Hepatitis B surface antigen (HBsAg) and hepatitis B core antigen(HBcAg) were detected by lmmunohistochemistry dyed by DAB. By Immunohistochemistry, no positive particles in LX-2 cells, a few of dark brown particles (HBsAg and HB-cAg) were found in cytoplasm and nucleoli of HepG2.2. 15 cells,a lot of clark brown particles were found in liver biopsies of patients with chronic hepatitis B. In vitro, there was no evidence that HBV can infect and express antigens in LX-2 cells.

To verify the role of introvascular ultrasound(IVUS) in diagnosis of atherosclerosis. MethodsINUS wasused to measure the position, quality and the thickness of the plaque in the elastic artery and biopsy materials. After using HE andHart stains, a micrometer was used to measuse the thickness of plaques and arterious walls. And electron microscopy was used to ob serve the pathological changs of the plaque. ResultsBy histological observation compared with IVUS, the conformable rate of plaque anmounted to 94%. The plaque could be seen in 12 sites of control group. Among 33 sites, 32 plaques were found pathologic exmination while IVUS showed 30 plaques in the experimental group. The two methods had a gcod correlation on the thick measurement of plaque and the media( r = 0.835, P＜0.05 and r = 0.814, P＜ 0.05). But they had a poor correlation on the measurement of the intirna( r = 0.055). ConclusionsBy pathological examination and the quantitative analysis, IVUS can obtain the common purpose in diagnosing atherosclerosis and measuring the media thickness. But it can not be used in measurement of the intima.

Objective To probe the safety of performing transradial artery percutaneous coronary intervention (PCI) for Allen test negative patients.Methods One hundred and six patients performed transradial artery PCI were enrolled in the study.The patients were divided into negative group (57 cases) and positive group (49 cases) according to Allen test results.Patients were performed ulnar artery angiography,deep palmar arch angiography and superficial palmar arch angiography.Ulnar artery diameter,deep palmar arch conditions,superficial palmar arch conditions,the frames counting through angiography on the side of ulnar vessel system,and hand ischemia after PCI (follow up for 3 months) was observed.Results There was no significant difference between negative group and positive group in parameters of ulnar artery diameter [(2.02 ±0.18) mm vs.(2.07 ±0.17) mm] and deep palmar arch and superficial palmar arch [85.96％ (49/57) vs.87.76％ (43/49),75.44％ (43/57) vs.81.63％ (40/49)] (P ＞0.05).The frames counting had no significant difference between negative group and positive group [(218.6 ± 63.6) frames vs.(180.8 ± 44.1) frames],but the ratio of the frames counting ≥380 frames had significant difference between negative group and positive group [14.04％(8/57) vs.2.04％(1/49)] (P ＜ 0.05).None of patients had been found to suffer from hand ischemia after PCI.Conclusion Transradial artery PCI is suitable for Allen test negative patients.

Objective To assess myocardial contractility in essential hypertention(EH) of different remoldings by means of circumferential midwall fractional shortening (mFs), relation between mFs and circumferential end-systolic stress(mFs-cEss). Methods The eject fraction(EF), circumferential end-systolic stress(cEss), fractional shortening(Fs), mFs was calculated in 115 EH patients and 29 normal controls. Relations in mFs-cEss, Fs-cEss between the subjects were analysed with linear correlation and linear regression. Results In using EF,Fs,Fs-cEss to assess chamber function of the patients with hypertension of four left ventricular remoldings, it shows apart from eccentric hypertrophy, the rest three remoldings were overestimative. MFs assessing myocardial contractility in four remoldings was lower than that of the controls, especially obvious in concentric hypertrophy patients(P

To review the research of block copolymer micelles as delivery system for poorly soluble(antineoplastic) carrier over the last 10 years,including the composition,preparation methods and factors which influence the loading efficiency,physicochemical properties,and targeting characteristics of block copolymer micelles.Block copolymer micelles are self-assembled structures formed from amphiphilic block copolymers by dispersing in aqueous media.The hydrophilic blocks of the copolymer form the outer shell of the micelle,while the hydrophobic blocks form the inner core,and the proper coreshell micellar architecture was constituted.Block copolymer micelles have a whole set of unique properties,such as small sizes,narrow particle size distribution,high drug loading capacities,and available disposition characteristics in the body.Block copolymer micelles have been found as promising drug carriers due to making poorly soluble antineoplastic lysis,toxicities and side effects decrease,bioavailability increase,and targeting the drugs to specific sites in a passive way or attaching ligands in an active way,which can be specifically(recognized) by receptors onto the surface of copolymers.

OBJECTIVE:To establish capillary GC method for the determination of residual organic solvents in artesunate,such as methanol and ethyl acetate.METHODS:Capillary GC was adopted and the temperature of flame ionization detector was 250℃.RESULTS:The linear range were 0.03～0.605 mg?mL-1 for methanol(r=0.999 8) and 0.05～1.002 mg?mL-1 for ethyl acetate(r=0.999 7).The average recovery rates were 98.7% for methanol(RSD=3.0%) and 99.1% for ethyl acetate(RSD=2.1%).Only the ethyl acetate was detected in samples.CONCLUSION:Established method is simple and accurate for the content determination of residual organic solvents in artesunate.

Objective To explore the effects of wearing ankle-foot orthoses (AFOs) on motor function in children with spastic cerebral palsy (SCP). Methods Fifty-two children with SCP were randomly divided into a wearing-for-training group (n = 16, group 1 ), a day-wearing group (n = 19, group 2) and a day-night-wearing group (n = 17, group 3). In addition to the conventional rehabilitative treatment given to all participants, the children in group 1 wore AFOs during movement training, and children in group 2 wore AFOs in the daytime for 6-8 h per day, while AFOs were applied to the children in group 3 for 24 hours a day except for cleaning and during certain training routines. All the treatments were continued for 2 months. Clinical assessments included the range of passive ankle dorsi-and plantarflexion (APROM) , modified Ashworth scale (MAS) ratings, and the D and E dimensions of the Gross Motor Function Measure (GMFM). All were performed before and after treatment. Results Before treat-ment, no statistically significant differences were found among the three groups in terms of APROM, MAS, or GM-FM. There were significant subsequent improvements in groups 2 and 3 when compared with group 1 in terms of APROM, MAS and GMFM results. Group 2's improvements in APROM and MAS results were not significantly better than those of group 3, but their average GMFM score improvements were significantly better. Conclusion Wearing AFOs in the daytime 6-8 hours per day is more effective in reducing spasticity and improving functional performance in children with SCP.

Objective To explore the prevalence of anxiety and depression among the medical staff of a children's hospital, and to understand their relationship with age, gender, educational background, family income, family harmony, position, marital status, experience, title, work pressure, weekly working hours, chronic diseases and stressful events.Methods The medical staff of a children's hospital were evaluated using Zung's self-rating anxiety scale (SAS) and self-rating depression scale (SDS).The scores were correlated with age, gender, educational background, family income, family harmony, position, marriage, working years, title, work pressure, weekly working hours, chronic diseases, and stressful events data collected about the same respondents.Results There were 320 respondents suffering from anxiety disorders and 392 experiencing depression among the 677 medical staff surveyed.There were 288 who suffered from anxiety associated with depression.There was a significant difference between the males and the females.Marital status, family harmony, chronic disease and stress events were all significant predictors of anxiety or depression.A univariate analysis showed that neither education nor family income had significant predictive power, but age, professional title,working years, working pressure and working in different departments were all significant predictors.Medical staff with different weekly working hours showed significantly different average levels of anxiety, but position had no such effect.Position was, though, significantly correlated with depression score, while weekly working hours were not.Multivariable logistic regression models showed that educational background, working pressure, family harmony, chronic disease and stressful events were all significant predictors of anxiety.Working pressure, family harmony and chronic disease were related to depression.Conclusion Significant anxiety and depression were observed among the medical staff of this children's hospital.Working pressure, family harmony and chronic disease are strong predictors of anxiety and depression.