OBJECTIVE: To establish a CGC method for the determination of pyridine in Potassium Dehydroandrograpol-ide Succinate. METHODS: The determination was performed on HP-INNOWAX capillary with column temperature at 100℃. The FID detector was used and the carrier gas was nitrogen. RESULTS:The linear range of Pyridine was 2.46～39.3?g?mL-1 and the average recovery was 98.07%,RSD=4.00(n=9).Its detection limit was 0.2?g?mL-1.CONCLUSION: The method is accurate and rapid, and applicable for the determination of the residual level of Pyridine in Potassium dehydroandrograpolide succinate.

OBJECTIVE: To establish the GC method for content determination of psoralen and isopsoralen in Buguzhi tincture.METHODS: The determination was performed on HP-1(30 m?0.53 mm,1 ?m) column with nitrogen as carrier gas and FID as detector,the inlet temperature was 260 ℃.The injector temperature was kept at 280 ℃ with diphenhydramine as internal standard.RESULTS:The linear range of psoralen were 4.82～48.22 mg?L-1(r=0.999 9) with an average recovery 103.11% (RSD=2.20%,n=6).The linear range of isopsoralen were 5.09～50.9 mg?L-1(r=0.999 9) with an average recovery of 100.98% (RSD=3.70%,n=6).CONCLUSION: This method is simple,rapid,accurate and reliable for the quality control of Buguzhi tincture.

0 05) CONCLUSION:It is advisable to use the external standard method to determine the concentration of valproate in human plasma

Objective To study the detection and significance of human papillomaviral DNA(HPV-DNA) in cervical disease. Methods The express of HPV-DNA were detected by hybrid capture 2 in the patients with chronic cervicitis ( n = 166), cervical intraepithelial neolasia ( GIN ) ( n = 89 ) and uterine cervix cancer ( UCC ) ( n = 23 ). Results The positive expression rate of HPV-DNA in GIN Ⅰ , Ⅱ , Ⅲ grade and UCC were 70.8% (17/24) ,79. 3% (23/29) ,88.9% (32/36) ,91. 3% (21/23). They were significantly higher than those in chronic cervicitis 30.7% (51/166) (P ＜ 0. 01 );As the development of cervical disease, HPV-DNA positive expression had increasing trend.Conclusion The detection of HPV-DNA had an important role for the early UCC screening,prevention and treatment.

The purpose of this study was to observe whether HBV can infect HSC in vitro. LX-2 cells, the human activated HSC cell line, were incubated with human serum containing HBVDNA at final concen-trations from 0.01 to 10 copies/cell and harvested after 24,48 and 72 hours. Hepatitis B surface antigen (HBsAg) and hepatitis B core antigen(HBcAg) were detected by lmmunohistochemistry dyed by DAB. By Immunohistochemistry, no positive particles in LX-2 cells, a few of dark brown particles (HBsAg and HB-cAg) were found in cytoplasm and nucleoli of HepG2.2. 15 cells,a lot of clark brown particles were found in liver biopsies of patients with chronic hepatitis B. In vitro, there was no evidence that HBV can infect and express antigens in LX-2 cells.

Objective:To observe the curative effect of modified amniotic membrane transplantation for agricultural pterygium.Methods: A total of 276 cases (276 eyes) with early pterygium were randomized divided into traditional group (138 cases) and modified group (138 cases) as random table. The traditional group was treated with pterygium excision in combination with autologous conjunctival transplantation; the modified group was treated with modified amniotic membrane transplantation; to observe the curative effect of the two groups.Results: After all of patients were followed up for 1 year, 122 cases were cured and 16 cases were recurrence, and the recovery rate was 88.41% in traditional group; 131 cases were cured and only 7 cases were recurrence, and the recovery rate was 94.93% in modified group and was better than traditional group; the difference between the two groups was statistical significant (x2=5.02,P<0.05).Conclusion: The modified amniotic membrane transplantation method is simple in operation, mini invasive for eye tissue, and can retain conjunctival tissue in utmost; it also can improve success rate of surgery and be an effective mean for treatment of pterygium.

Objective To discuss the cause of death in patients with spinal cord injury from Tangshan earthquake 37 years latter, and compare them with the survey before. Methods The causes of death of the spinal cord injury patients lived in 1 sanatorium and 1 rehabilitation village in Tangshan city, and 6 sanatoriums in villages from 2003 were surveyed. The questionnaire included their general condition, the main cause of death, time of death, age at death, etc., and the findings in 1988 and 2003 were compared. Results Uremia and pressure sore infection were the most major causes of death, in which pressure sore infection was the first cause in the village, and uremia was the first both in the village and city, and the epidemiology of them decreased than before. Cardiovascular disease and cerebrovascular disease were the second and third causes of death respectively. The epidemiology of malignancies increased in this survey, and the longest life time of patients with cervical spinal cord injury was 36 years. Conclusion The top causes of death in spinal cord injury patients from Tangshan earthquakeare uremia, pressure sore infection, cardiovascular disease and cerebrovascular disease. 37 years after Tangshan earthquake, the cause of death and the life expectancy of patients with spinal cord injury have been close to general levels.

Objective To explore the correlation of metabolic disorder of newly diagnosed type 2 metabolism(T2DM) and nonalcoholic fatty liver disease (NAFLD).Methods A total of 117 patients with newly diagnosed T2DM in Beijing Ditan Hospital Affiliated to Capital Medical University from June 2014 to June 2015 were enrolled.Patients were divided into two groups:T2DM with NAFLD of 57 cases and T2DM without NAFLD of 60 cases.Body mass index (BMI),waist circumference (WC),liver and kidney function,serum lipid,glycosylated hemoglobin A1c (HbA1c),fasting glucose,fasting insulin and C-peptide (FCP) were detected.The insulin resistance and β-cell function were assessed by homeostasis model assessment insulin resistance (HOMA-IR) and HOMA-β.Results The incidence of NAFLD in T2DM patients was 51％ (that was,60 cases of T2DM patients with NAFLD),compared with 2TDM group,the metabolic index of T2DM without NAFLD was significantly increased (blood triglyceride (TG):(2.58 + 1.8) mmol/1 vs.(1.22 + 0.4) mmol/l,fasting blood glucose:(11.5+6.1) mmol/l vs.(9.2+4.3) mmol/l,serum uric acid:(465.3+65) umol/l vs.(325.3+72) umoL/l;P =0.03,0.03,0.02);HOMA-IR was more serious ((4.9 ± 2.8) vs.(3.8 ± 2.7);P =0.03);insulin β-cell function was compensatory more obvious ((49.2 ± 27) vs.(29.5 ± 18);P =0.02);prevalence rate of obesity,hypertension and diabetic ketoacidosis (DKD),diabetic retinopathy (DR),diabetic peripheral neuropathy(DPN) were significantly increased(P＜0.05).Logistic regression analysis showed that,smoking(OR=1.405,95％CI:1.262-1.567),male(OR=1.037,95％CI:1.015-1.063),BMI(OR=1.113,95％CI:1.278-2.531),WC (OR =1.624,95％ CI:1.162-1.761) and TG (OR =1.823,95％ CI:1.2822.563) were risk factors of NAFLD (P ＜ 0.05).Conclusion T2DM patients with NAFLD have severer insulin resistance than those without NAFLD.The β-cell function of those patients is compensatory increased.NAFLD prevalence rate significantly increase in patients with obesity,especially in male diabetic patients who smoking.

OBJECTIVE:To explore the effects of folic acid combined with vitamin B12 on the vascular cognitive impairment (VCI)and homocysteine(Hcy)of patients with hypertension and cerebral stroke. METHODS:80 VCI patients with hypertension and cerebral stroke were regarded as observation group,and another 20 healthy volunteers were regarded as healthy control group. Observation group was treated with enhanced homogenized meal 300-500 ml,3 times a day+Folic acid tablet 5 mg,3 times a day+Vitamin B12 tablet 25 μg,3 times a day. Observation groups were treated for continuous 2 months with no nasal administration by themselves. The Mini-Mental State Examination(MMSE)score,the level of folic acid,vitamin B12 and Hcy before and after treat-ment were observed and compared with healthy control group,and the incidence of adverse reactions was recorded. RESULTS:Af-ter treatment,MMSE score,the level of folic acid,vitamin B12 in observation group were significantly higher than before and low-er than healthy control group,the level of Hcy was significantly lower than before and higher than healthy control group,the differ-ences were statistically significant(P<0.05). The incidence of adverse reactions was 5.00%. There were no serious adverse reac-tions. CONCLUSIONS:Folic acid combined with vitamin B12 can reduce the cognitive impairment degree of patients with hyperten-sion,cerebral stroke and vascular cognitive impairment and level of Hcy,with good safety.

OBJECTIVE:To establish a HS-GC method for determining methanol and ethanol in human plasma METHOD_S:An external standard method was used The stationary phase was a glass column packed with GDX-102 The carrier gas was nitrogen The column temperature was 140℃ and both the injector temperature and the detector temperature were 200℃ RE_SULTS:A good linearity was obtained in the range of 0 2～7 5g/L of methanol and ethanol The within-day RSD was less than 4% and the between-day RSD less than 5 5% The recoveries were (100 9?3 8)% for methanol,(101 5?3 4)% for et_hanol,respectively The limits of detection for both methanol and ethanol were less than 0 01g/L CONCLUSION:This method is simple,rapid and precise