0.05).The major germ of subgingival flora of the patients with DM-2 was anaerobe. Amount of black germs and Capnocytophaga was positively correlated with the level of FPG or GHb in the patients with DM-2(P

Objective To study the effect of Yiqimingmu pill on ATPase and LD of retinal ischemia rabbit, and probe into its mechanism. Methods Thirty-two Japanese large-ear white rabbits were divided into four groups, normal group (A group), model group (B group), comparison group (C group) and treated group (D group). Retinal ischemia was made in B, C and D group. After the treatment of one month, testing the correspondent indexes and studying with statistics. Results After the treatment of one month, in B and C group, the content of ATPse was lower, while the content of LD was higher compared with A group. Conclusion Yiqimingmu pill is reliable in the treatment of retinal ischemia, and superior to Buzhongyiqi wan.

Objective To investigate the effects of astragalus membranaceus and angelica sinensis on grafted cochlea stem cells apoptosis in hearing imparied rats.Methods The hearing impaired rats were divided into 3 groups:cochlea stem cells group(45 rats),astragalus membranaceus and angelica sinensis group(45 rats),and the control group(35 rats).Cochlea stem cells only were grafted into the first group cochlea regions,mixture of stem cells and astragalus membranaceus and angelica sinensis were grafted into that of the second group.Saline was grafted into that of control rats.Observed was apoptosis at 1 day,3 days,5 days,10 days,15 days after the transplantation by CASPASE-3 and Bcl-2 immunofluorescence.Results After transplantationn 1 day,3 days,5 days,10 days,and 15 days of five time,for the cochlea stem cell group,the rates of apoptotic cells were 27.84%,49.37%,44.78%,39.37%,and 34.82%,respectively.The rates of astragalus membranaceus and angelica sinensis group were 18.35%,26.92%,23.18%,21.38%,and 18.19%,respectively.The percent of grafted cochlea stem cells apoptosis in astragalus membranaceus and angelica sinensis group was much lower than that in cochlea stem cells group and control group.The majority of apoptotic cells occurred within three to five days after transplantation.Conclusion The majority of apoptotic cells occur within three to five days after transplantation in the hearing impaired rats.The astragalus membranaceus and angelica sinensis could reduce the apoptotic rates of transplanted stem cells.

Cyclic voltammetry shows that, in a supporting ele ctrolyte of NaOH,riboflavine tetrabutyrate（RT）has a pair of cathodic and anodi c peaks at Hg electrode. The peak potentials Epc=Epa=-0.64　 V（vs.Ag/Agcl）.　The adsorptive characteristics of RT are explored in detai ls with various methods.　The adsorbed species is most probably neutral molecule of RT.The saturated adsorption amount of RT at Hg electrode is 5.42×10-11 　mol/cm2 and every RT molecule occupied 3.06　nm2. On the surface of HMDE ，the adsorption of RT obeys Frumkin dasorption isotherm. The adsorptive coeffic ient β，the attractive factor γ are 4.60×105，1.10 and the adsorption free energy ΔG, is　－32.30　kJ/mol, respectively. The electrode proc ess kinetic behavior has also been studied by using cyclic voltammetry. Under th e experimental conditions the rate constants for electron transfer are determine d. The values of ks are in good agreement with literature′s results.

Purpose The incidence of cesarean scar pregnancy (CSP) shows a rising trend in recent years, and misdiagnosis or improper treatment of it can cause serious consequences. In this paper, we analyzed the characteristics of MRI and ultrasonography of CSP to provide a basis for choice of clinical imaging techniques. Materials and Methods Fifty-eight patients with cesarean scar pregnancy were studied retrospectively, all the cases underwent preoperative color Doppler ultrasound (trans-vaginal or together with trans-abdominal), and 31 cases were examined with MRI, the accurate imaging features and diagnosis rate of each was analyzed.Results 48 patients (82.8%) were diagnosed accurately by color Doppler ultrasound, including 20 cases of out-growth type (95.2%, 20/21), 18 cases of inner-growth type (90.0%, 18/20) and 10 cases of mass type (58.8%, 10/17); 27 cases (87.1%) were diagnosed accurately by MRI, including 8 cases of out-growth type (100.0%, 8/8), 4 cases of inner-growth type (66.7%, 4/6), and 15 cases of mass type (88.5%, 15/17).Conclusion The diagnostic accuracy of sonography for CSP is close to that of MRI, thus can act as the first choice; MRI can be chosen for combine examination to improve the accurate diagnostic rate when the CSP case is mass type detected by ultrasonic findings which is difficult to be diagnosed.

Objective To establish an HPLC method for the determination of artesunate in artesunate and amodiaquine hydrochloride tablets. Methods WondaSil C18-WR column was used with mobile phase consisted of acetonitrile:phosphoric acid aqueous solution(adjust pH to 3,gradient elution);wavelength was 210 nm; flow rate was 1 mL/min and the column temperature was 30℃.Results The standard curve was linear in the range of 0.2~3.2 mg/mL(r=0.9997), average recoveries were 99.0%(RSD=1.35%, n=6).Conclusion The method is accurate and sensitive, and it can be used to control the quality of artesunate and amodiaquine hydrochloride tablets.

Objective: To explore the contents of hypericin from different parts of Hypericum sampsonii. Methods : The hypericin in Hypericum sampsonii and H. Officinalis were measured by HPLC with Novapak C 18 column, mobile phase(methanol∶ethanol∶0.1mol?L -1 NaH 2SO 4=200∶300∶100), flow rate 1.0mol?L -1 , column temperature at 30 ?C , wavelength at 588nm. Results:Contents of H. Sampsonii in flower and flower itself were equal content to H. officinalis. Conclusion:It is possible to sift out the plant of H. Sampsonii with more Hypericin.

OBJECTIVE:To focus on the situation of clinical pharmacists particpating in the clinical drug treatment consulta-tion in China. METHODS:With the keywords of“clinical pharmacists”,“consultation”and others,retrieved from China Hospital Knowledge Database (CHKD),Wanfang Database and China BioMedical Literature Service System (SinoMed),the situation of clinical pharmacists particpating in the clinical drug treatment consultation in China was concluded and summarized. RESULTS:A total of 186 literatures were included,invloving 114 effective literatures. 2 606 cases were clinical pharmacists particpating in the clinical drug treatment consultation,2 257 cases were all or part of adoption of clinical pharmacists’suggestions,the execution lev-el of clinicians for clinical pharmacists’consultation suggestions reached 93.02%. After carrying out the clinical pharmacists’con-sultation suggestions,patients’condition improved or cured reached 2 290 cases,with the effective rate of 94.47%. CONCLU-SIONS:Clinical pharmacists participating in the clinical consultation has certain function,however,the working method and mode need to be further explored and standarized.

Objective To evaluate the role of p38 mitogen-activated protein kinase (p38MAPK) in the spinal cord in the development of persistent postoperative pain evoked by skin/muscle incision and retraction (SMIR) and the relationship with Toll-like receptor 4 (TLR4).Methods One hundred and twenty male SpragueDawley rats,weighing 200-250 g,aged 2 months,in which intrathecal catheters were successfully implanted,were randomly divided into 5 groups (n =24 each) using a random number table:sham operation group (group S),SMIR group,SMIR + dimethyl sulfoxide (DMSO) group (group DMSO),SMIR + p38MAPK inhibitor SB203580 group (group SB203580) and SMIR + TLR4 small interference RNA (siRNA) group (group TLR4siRNA).The rats were anesthetized with intraperitoneal chloral hydrate 400 mg/kg.The skin and superficial muscle of the medial thigh were incised and a small pair of retractors inserted.This tissue was retracted for 1 h causing potential stretch of the saphenous nerve.2％ DMSO 10 μl and SB203580 5 μg were injected intrathecally at 30 min before operation and 1-12 days after operation in DMSO and SB203580 groups,respectively.TLR4siRNA 2 μg was administered intrathecally at 1 day before operation and 1-12 days after operation once a day in group TLR4siRNA.Mechanical paw withdrawal threshold to von Frey filament stimulation (MWT) was measured at 1 day before operation and 1,3,7,12 and 22 days after operation.Four rats in each group were sacrificed after measurement of MWT at each time point,and the L4-6 segments of the spinal cord were obtained for detection of the expression of phosphorylated p38MAPK (p-p38MAPK) by Western blot analysis.Results Compared with group S,MWT was significantly decreased after operation,and the expression of p-p38MAPK was up-regulated after operation in SMIR and DMSO groups.Compared with group SMIR,MWT was significantly increased after operation,and the expression of p-p38MAPK was down-regulated after operation in SB203580 and TLR4siRNA groups,and no significant changes in MWT and p-p38MAPK expression were found at each time point in group DMSO.Conclusion TLR4-triggered activation of p38MAPK in spinal cord is involved in the development of SMIR-evoked persistent postoperative pain in rats.