1.The clinical observation of mannatide treatment with burn infection
Hongyan LIU ; Wenlian HUANG ; Wenming XIAO
Chinese Journal of Biochemical Pharmaceutics 2014;(2):133-134
Objective To observe the clinical effect of mannatide treatment with burn infection. Methods 100 burn patients admitted to Sichuan Nanchong Central Hospital from May 2009 to July 2012 were randomly divided into control group and treatment group, each group had 50 cases. Both of two groups were given conventional treatment, treatment group were treated with intravenous mannatide,control group were injected recombinant human growth hormone. Results The average length of stay in treatment group was signiifcantly shorter than control group (P=0.042). The clinical total effective rate, serum IgA, IgG, IgM and other indicators of immune function were improved signiifcantly compared with control group (P=0.037). There was no statistical signiifcance in blood protein, transferrin, prealbumin, glucose, insulin, urinary sodium, urinary potassium, chloride and other indicators of patients between two groups. Conclusion Mannatide can effectively enhance immune function, promote wound healing, shorter hospital stay and improve the clinical efifcacy.
2.Management and Practice of Quality Control in the Pharmacy Intravenous Admixture Service in Our Hos-pital
Jinyan XIAO ; Qiong YANG ; Derong PU ; Lishi CHEN ; Hongyan LI
China Pharmacy 2017;28(13):1805-1809
OBJECTIVE:To provide reference for the realization of normalization and standardization of quality control and management of Pharmacy intravenous admixture service(PIVAS). METHODS:The management and effectiveness of PIVAS quali-ty control in our hospital were introduced from aspects of the establishment of internal quality control system,the key indicators and effects of quality control. RESULTS:According to checking items from aspects of examining once in a month,on prescription checking,preparation,infection control,equipments,drugs,consumable materials,sanitation;holding 1 monthly quality control reviews;and analyzing unqualified indexes and existing security risks,the quality control of PIVAS had achieved good results. The qualified percentage of quality control index was increased from 84%(Jan. 2016)to 90%(Jun. 2016),error rate in the same peri-od was decreased from 0.579‰ to 0.099‰. CONCLUSIONS:Developing quality control of the implementation of detailed quality control key indexes for the whole process of intravenous infusion in PIVAS can effectively regulate the position working process,re-duce preparation error and ensure the quality of infusion preparation.
3.Progress in sodium channelopathies and biological functions of voltage-gated sodium channel blockers.
Hongyan WANG ; Meng GOU ; Rong XIAO ; Qingwei LI
Chinese Journal of Biotechnology 2014;30(6):875-890
Voltage-gated sodium channels (VGSCs), which are widely distributed in the excitable cells, are the primary mediators of electrical signal amplification and propagation. They play important roles in the excitative conduction of the neurons and cardiac muscle cells. The abnormalities of the structures and functions of VGSCs can change the excitability of the cells, resulting in a variety of diseases such as neuropathic pain, epilepsy and arrhythmia. At present, some voltage-gated sodium channel blockers are used for treating those diseases. In the recent years, several neurotoxins have been purified from the venom of the animals, which could inhibit the current of the voltage-gated sodium channels. Usually, these neurotoxins are compounds or small peptides that have been further designed and modified for targeted drugs of sodium channelopathies in the clinical treatment. In addition, a novel cysteine-rich secretory protein (CRBGP) has been isolated and purified from the buccal gland of the lampreys (Lampetra japonica), and it could inhibit the Na+ current of the hippocampus and dorsal root neurons for the first time. In the present study, the progress of the sodium channelopathies and the biological functions of voltage-gated sodium channel blockers are analyzed and summarized.
Animals
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Channelopathies
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physiopathology
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Hippocampus
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drug effects
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Neurons
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drug effects
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Neurotoxins
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pharmacology
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Venoms
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chemistry
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Voltage-Gated Sodium Channel Blockers
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pharmacology
4.Study on practice teaching of medical imaging in clinical medicine program
Furong LV ; Fajin LV ; Zhibo XIAO ; Hongyan CHEN
Chinese Journal of Medical Education Research 2006;0(12):-
To improve the quality of practice teaching in medical imaging,we explored the teaching methods on the basis of quality-oriented education,combining the special nature of the medical imaging and the clinical medicine program.
5.The use of comparative imaging in teaching of medical imaging
Furong LV ; Fajin LV ; Zhibo XIAO ; Hongyan CHEN
Chinese Journal of Medical Education Research 2003;0(02):-
Comparative imaging can be used to analyse and compare diseases in different respects,which can not only conduce to students’transversal contrast of knowledge they have learned, but also improve the teaching effect of medical imaging and the students’clinical skills,and at the same time,lay a solid foundation of the research for future.
6.The research on the assessment methods of residents/specialist training
Liangyuan TANG ; Hongyan CHEN ; Guosheng REN ; Mingzhao XIAO
Chinese Journal of Medical Education Research 2003;0(02):-
Our hospital has set up a relatively complete assessment methods after nearly 10 years of residents/medical specialist training, including the day-to-day assessment, periodic assessment,comprehensive assessment,and completed the corresponding management evaluation.
7.Medical specialists education program——the platform for the growth of 21 century medical specialists
Guosheng REN ; Hongyan CHEN ; Tianyou LUO ; Mingzhao XIAO
Chinese Journal of Medical Education Research 2003;0(02):-
Setting up medical specialists education system is the requirement of the re-form and development of medical sciences and health causes and is a must to train medical specialists. It is of significance to perfect medical education and standardize residency training and management in China,so as to promote medical education and talents management to be in line with the world.
8.Researches on the medical english ability in the residency training project
Hongyan CHEN ; Tianyou LUO ; Mingzhao XIAO ; Liangyuan TANG ; Guosheng REN
Chinese Journal of Medical Education Research 2003;0(04):-
We have established the model of English ability training on the residency training project,and have provided the systematic training plan to 29 residents who were employed in 2005.This paper summarizes the effect and points out the further reform perspectives.
9.Determination of Nine Aconitum Alkaloids in Xiaohuoluo Pills with Quantitative Analysis of Multi-compo-nents by Single Marker
Hongyan CHEN ; Xiao SUN ; Xiaolong ZHANG ; Chenxu YAO ; Yan JIANG
China Pharmacist 2017;20(9):1545-1549
Objective:To develop a method of quantitative analysis of multi-components by single marker( QAMS) for nine kinds of alkaloids in Xiaohuoluo pills. Methods: An HPLC-QTOF-MS method with an Agilent ZORBAX Extend-C18 RRHT(2. 1 mm × 50 mm,1. 8 μm) column was applied. The flow rate was 0. 21 ml·min-1 . The column temperature was 30 ℃. The mobile phase was methanol (A)-water (B;containing 0. 1% formic acid and 2. 5 mmol·L-1 ammonium acetate) with gradient elution. The aconitine was used as the internal standard, and the relative correction factor ( RCFs) of hypaconitine, mesaconitine, benzoylaconine, benzoyl-hypaconine, benzoylmesaconine, aconine, hypaconine and mesaconine was respectively established, and the reproducibility inspection on the RCF was performed. The contents of the other 8 kinds of aconitum alkaloids were calculated according to the RCF. At the same time, an external standard method ( ESM) was performed for the content determination of the nine alkaloids. The results of the two methods were compared. The feasibility and accuracy of the QAMS method were verified. Results:Within a certain range,the RCF of hypacontine,mesacontine, benzoylaconine, benzoylhypaconine, benzoyl mesaconine, aconine, hypaconine and mesaconine to aconitine was 1. 736,1. 979,1. 0471,0. 9242,1. 2901,1. 3078,1. 2859,and 1. 0948,respectively. The QAMS method was established for determi-ning alkaloids. There were no significant differences between the results of the QAMS method and those of the external standard method ( ESM) . Conclusion:With the validation of methodology, the method established in our study can be used for the content determina-tion of aconitine, hypaconitine, mesaconitine, benzoylaconine, benzoylhypaconine, benzoylmesaconine, aconine, hypaconine and mesaconine in xiaohuoluo pills.
10.Osthole reduced Aβ synthesis by up-regulatingmiRNA-107 in neurons transfected with APP595/596 gene
Honghe XIAO ; Yanan JIAO ; Yuhui YAN ; Hongyan LI ; Jingxian YANG
Chinese Pharmacological Bulletin 2017;33(8):1079-1085
Aim To investigate the neuroprotective effects of osthole(Ost)on the primary cultured cortical neurons transfected with APP595/596 gene and its underlying mechanism.Methods Neonatal mouse cortical neurons were transfected with APP595/596 gene to establish AD cell models for the further study.Then,the cell viability was detected by CCK-8 assay,and the leakage of lactate dehydrogenase(LDH)was assayed by LDH kit to evaluate the injury degree.Transferase-mediated nick end labeling(TUNEL)was used to evaluate the cell apoptosis.The expression of β-amyloid peptide(Aβ)and β-site APP cleaving enzyme 1(BACE1)was measured by immunofluorescence,while the miRNA-107 was measured by RT-PCR.Results Compared to model group,Ost could significantly improve the neurons viability,decrease the LDH release and prevent the apoptosis.Ost also inhibited the expression of Aβ and BACE1 at protein level,while enhanced the expression of miRNA-107 at gene level.Conclusion Ost plays a neuroprotective role in neurons transfected with APP595/596 gene in part through up-regulating miRNA-107.