Objective To explore the effect of follow-up by telephone calls on the medication compliance of patients with cancer pain.Methods Two hundred and forty hospitalized cancer patients suffering from moderate to severe cancer pain were included in the study. All the patients were provided with education on pain treatment before discharge.Then they were randomly assigned to receive telephone follow-up or routine nursing after discharge.The medication compliance were compared 2 months after discharge.Result The medication compliance in the observation group was significantly better than that in the control group.Conclusion Telephone follow-ups can improve medication compliance in patients with cancer pain.

【Objective】To investigate the therapeutic effect of Chinese herbal medicine combining with the therapeutic instrument of amblyopia for the treatment of amblyopia.【Methods】One hundred amblyopia patients were divided into group A(94 eyes in 50 cases) and group B(95 eyes in 50 cases) by the method of simple randomization.Patients in group B wore appropriate glasses,and covered the eye with a better sight when the sight difference over 2 lines or covered the eyes alternatively when the sight difference below 2 lines.Meanwhile,patients in group B were treated by the curative instrument of amblyopia(micro-lightbrush).Patients in group A were treated with herbal medicine with the actions of strengthening spleen and tonifying kidney,tonifying essence and nourishing blood(Radix Codonopsis,Rhizoma Dioscoreae,Fructus Lycii,Fructus Mori,Semen Cuscutae,Semen Cassiae,Rhizoma Polygonati,Radix Rehmanniae Preparata,Radix Paeoniae Alba,pericarpium Citri Reticulatae,Radix Puerariae) additonally.Fifteen days constituted one treatment course and an interval of 5 days was between two courses.The two groups were treated for one successive year.After treatment,the total therapeutic effect,effect in different age groups and effect in patients with different degrees of amblyopia were compared between the two groups.【Results】In group A,69(73.4%) eyes were cured,21(22.3%) effective and 4(4.3%) ineffective,and 58(61.1%),25(26.3%) and 12(12.6%)in group B,respectively.The total therapeutic effect in group A was superior to that in group B(P0.05),but the effect was superior to that on patients aged 9 years and older in the two groups(P0.05).However,for the patients with severe amblyopia,the effect was better in group A than that in group B(P

Objective:To investigate the clinicopathologic features and diagnostic methods of intraductal papillary(IP)lesions of the breast.Methods:The clinical data of 1 679 patients who had been pathologically diagnosed as having IP were analyzed retrospectively.Results:Nipple discharge is the most common clinical symptom of IP. According to the postoperative pathological results, IP patients were divided into solitary IP group and multiple IP group . Nipple discharge was more common in solitary IP group ( P<0.05). Bloody discharge and duct nipple discharge and discharge in unilateral breast were the most common clinical symptom of IP, though the difference was not significant ( P>0.05). The accuracy of diagnosing IP by fiberoptic ductoscopy(FDS) was 88.66% which was significantly higher than that of breast ultrasound and mammography. Solitary IP patients with nipple discharge undergoing FDS before surgery and lesions were more often detected located in general or level Ⅰ-Ⅱ duct system ( P<0.05). Postoperative follow-up did not show malignant transformation in solitary IP group, while there were 4 cases in the multiple IP group of malignant transformation and higher recurrence rate of IP ( P<0.05). Conclusions:Bloody discharge, single duct nipple discharge and unilateral breast involving 1esion and 1esions located in the general or level Ⅰ-Ⅱ duct system are common clinical features of IP. FDS has advantages in the diagnosis of IP with nipple discharge. For fear of recurrence , and malignant transformation in multiple IP, active postoperative follow-up should be carried out.

Objective To analyze the characteristics of TCM syndromes of primary glaucoma to provide evidence for clinical treatment.Methods The disease course, eye pressure and clinical symptoms of 114 patients (120 eyes) with primary glaucoma were studied to analyze their TCM syndrome characteristics and distribution regularities.Results (1) In the primary glaucoma, the patients of liver excess syndrome were at the utmost and those of heart and lung qi deficiency syndrome the least. (2)The disease course of liver excess syndrome group was the shortest and that of spleen and kidney deficiency syndrome the longest (P

Objective The study was aiming at elucidating the rale of BSP over-expression in enhancing osseous metastasis of breast cancer cells,in order to search for new drug targets for prevention and treatment of such metastasis.Methods Chicken embryo allantois membrane(CAM)was used in the experiment.Breast cancer cells were inoculated on the superior surface of CAM sac,then the vascular tissues were collected in lower sac after incubation.The human specific Alu gene was amplified by PCR with genomic DNA extracted as template.Breast cancer cells were incubated with bone,which had becn treated with collagenase and trypsinase,and then OD_(488) of breast cancer was assessed to evaluate the adhesive affinity of breast cancer cells to bone.Results The findings of CAM assay and PCR amplifica- tion using human Alu primers and genomic DNA showed that Alu gene was amplified from 4 of 5 in MDA-MB-231BO(pI)group and 3 of 5 in MDA-MB-231BO(pID)group,but not amplified in control group.In bone adhesion assay,the OD_(488) of digested bone after MDA- MB-231BO(pI)and MDA-MB-231BO(pID)adhering to bone were 0.58?0.046 and 0.49?0.039,respectively,which were larger than that of MDA-MB-231BO(0.26?0.021,P

Objective ToexpresschimericantigenofHCVwithmultipleimmunodominantepitopes inE .coliforimprovingthequalityofreagentsforHCVscreening .Methods Thegenefragmentencoding HCVchimericantigenwasobtainedbymolecularcloningmethodandclonedintopQE 30 plasmidforex pressioninE .coliM 15 .TheexpressedchimericantigenwaspurifiedbyNi NTAandcoatedonELISA platestoanalyzeitssensitivityandspecificity .Results Thechimericantigenof 5 30 0 0washighlyex pressed .ELISAassayofserumsamples (including 18HCVpositiveand 17negativesera)indicatedthatthe HCVchimericantigenhadhighsensitivityandspecificity .Conclusions ChimericantigenofHCVwith typicalimmunodominantepitopescanbeusedtodevelopgoodreagentsforHCVimmunoassay .

OBJECTIVE:To observe clinical efficacy and safety of Chaihu shugan powder combined with clonazepam in the treatment of liver qi stagnation type anxiety. METHODS:96 patients with liver qi stagnation type anxiety were randomly divided in-to observation group and control group,with 48 cases in each group. Control group was given Clonazepam tablet with initial dose of 0.5 mg,increasing to 4.0 mg gradually,tid;observation group was additionally given Chaihu shugan powder 300 ml,bid,on the basis of control group. Both groups were treated for 6 weeks. Clinical efficacy of 2 groups were observed,and HAMA and SAS were observed before and after treatment;the incidence of ADR were compared between 2 groups. RESULTS:The total effective rate of observation group(97.92%)was significantly higher than that of control group(83.33%),with statistical significance(P<0.05). There was no statistical significance in HAMA and SAS between 2 groups before treatment(P>0.05);HAMA and SAS of 2 groups decreased significantly 1,3 and 6 weeks after treatment,and the observation group was lower than the control group, with statistical significance(P<0.05). There was no statistical significance in the incidence of ADR between 2 groups(P>0.05). CONCLUSIONS:Chaihu shugan powder combined with clonazepam is effective,improve patient anxiety and safe in the treatment of liver qi stagnation type anxiety.

Objective To construct the eukaryotic expression vector coding HCV gene E2 fused with His-Tag, and to express fused protein in CHO cells for investigating the function of HCV envelope protein E2. Methods The gene encoding HCV envelope protein E2 was amplified from pBRTM/HCV1-3011, a plasmid containing the cDNA of HCV's ORF, by polymerase chain reaction (PCR) method and cloned into the vector pET28(a) containing His-Tag to obtain the fused HCV envelope protein E2 gene fused with His-Tag. The fused gene was cloned into pcDNA3.1 to construct the recombinant plasmid pcDNA3.1-His-E2, which will express the E2 protein, fused with His tag. This recombinant plasmid was transfected into CHO cells by Lipofactamine 2000 reagent. The fused protein was identified by indirect immunofluorescence (IIF) and Western-blot (WB) methods. Result The positive results were obtained when the fused protein of HCV E2 with His-Tag were identified by IIF and WB methods. Conclusion The eukaryotic expression vector pcDNA3.1-His-E2 was constructed successfully and the fused proteins were expressed in cells.

Objective To constitute a higher expression system and to obtain the breast cancer cell strains in which BSP is stably expressed. Methods hBSP gene was subcloned from pB-hBSP vector by PCR. The PCR fragment was inserted into the eukaryon expression vector, pIRES2-EGFP, which allow exogenous protein and EGFP to express respectively. The recombinant vector, pIRES2-hBSP-EGFP, was transfected into human breast cancer cells with Lipofectamine TM 2000. The expression of symbol protein EGFP, could be conveniently observed with fluorescent microscope. Results The recombinant pIRES2-hBSP-EGFP plasmid was constituted and successfully transfected into breast cancer cells. In the breast cancer cell strain hBSP and EGFP were expressed. Conclusion The successful constitution and transfection of hBSP and EGFP nonfusion expression vector laid a foundation for the further study on the effect of BSP on breast cancer metastasizing to bone in vivo or in vitro.

Objective Recent researches show that bone sialoprotein is closely related with metastasis of breast cancer to bone. In order to investigate the effect of bone sialoprotein in the course of metastasis of breast cancer to bone, the following study was undertaken. Methods The growth curve, clone formation and adherence of breast cancer cells experiments were carried out to study the effect of hBSP over-expression on biological behaviors of breast cancer cells. Results It was found that breast cancer cells MDA-MB-231BO(pI) and MDA-MB-231BO(pID) stably transfected with hBSP cDNA were not inhibited through proliferation assay of breast cancer cells. The activity and tumorigenicity of breast cancer cells with hBSP over-expression were enhanced. Comprising with MDA-MB-231BO non-transfected the colony formation percentage of breast cancer cells MDA-MB-231BO(pI) and MDA-MB-231BO(pID) increased from 8.90%?0.53% to 15.6%?0.94% and 12.8%?0.77%. The OD_ 620 value of adherence of breast cancer cells MDA-MB-231BO(pI) and MDA-MB-231BO(pID)increased from 0.29?0.017 to 0.52?0.031 and 0.43?0.026 respectively in cells attachment assay, which suggested that over-expression of hBSP enhances breast cancer cell MDA-MB-231BO adherence and attachment property. Conclusion The study results suggested that hBSP over-expression had autocrine effect on breast cancer cells and could enhance the ability of breast cancer cells metastasizing to bone.