BACKGROUND:Proinflammatory transcription factors and inflammatory factors play an important role in the occurrence of alcoholic liver disease. However, early growth response factor 1 is one of the key factors of starting inflammation. OBJECTIVE: To investigate and analyze the effects of silymarin on early growth response factor1 in rat models of alcoholic fatty liver. METHODS:The rat models were established using the methods of feeding with high fat diet and intragastricaly administering alcohol, in total 8 weeks. Silymarin intervention at high and low doses (200,100 mg/kg) was given after each gavage. The normal group was set as comparison. RESULTS AND CONCLUSION:Serological indicator detection and hematoxylin-eosin staining results showed that compared with the model group, the body weight of rats was increased (P < 0.05); serum aspartate aminotransferase, alanine aminotransferase activity, early growth response factor 1 and tumor necrosis factor-α expression in the liver tissue were decreased (P < 0.05); pathological grading results were superior in the high-dose silymarin group to in the other groups (P < 0.01). The results confirm that high dose of silymarin can protect the liver function of rats, reduce the occurrence of liver function damage, which may be associated with the inhibition on the early growth response factor 1 in the body of rats, thereby reducing the tumor necrosis factor-α formation, but the specific mechanism remains to be further studied.

Objective To investigate hepatitis B virus surface antign (HBsAg) quantitative value quantitatively in serum of chronic hepatitis B virus (HBV) infection patients with different clinical stages,at the same time,to explore the correlation between HBV DNA,patient's age and HBsAg quantitative values.Methods Collected 774 cases without antiviral treatment of chronic HBV infection from our hospital,according to the clinical features,divided cases into six groups:chronic HBV carrier group (102 cases),inactive HBsAg carrier group (211 cases),hepativis B virus e antigen (HBeAg) positive chronic hepatitis B group (236 cases),HBeAg negative chronic hepatitis B group (114 cases),HBeAg positive hepatitis B cirrhosis group (52 cases),HBeAg negative hepatitis B cirrhosis group (59 cases).Used chemiluminescence immunoassay particles analysis to determine HBsAg quantitative value in serum in patients,used real-time fluorescent quantitative polymerase chain reaction method to determine HBV DNA quantitative value in patients,and then compared differences among groups.Results HBsAg quantitative value from high to low respectively were chronic HBV cartier group,HBeAg positive chronic hepatitis B group,HBeAg negative chronic hepatitis B group,inactive HBsAg carrier group,HBeAg negative hepatitis B cirrhosis group,HBeAg positive hepatitis Bcirrhosis group,the median quantitative value of HBsAg were [7.80 (6.69-8.32),7.11 (5.42-8.27),6.57 (5.66-7.53),6.38 (4.39-7.40),6.22 (4.84-6.91),6.13 (5.48-7.01)] ; positive correlation was found between HBsAg quantitative value and HBV DNA in HBeAg positive chronic hepatitis B group and HBeAg negative chronic hepatitis B group (r =0.714,0.390,P ＜ 0.01); negative correlation was found between HBsAg quantitative value and age in chronic HBV infection(r =-0.416,P ＜ 0.01) ; Monitored HBsAg quantitative value of inactive HBsAg carriers after the age 40 had important clinical value.Conclusions HBsAg quantitative value is different in the various phases of chronic HBV infection.HBV DNA levels and age are related with HBsAg quantitative value.HBsAg quantitative value should be monitored in inactive HBsAg carriers after the age 40.

Objective To explore the anti-hyperuricemia activity of bergenin in the model of hyperuricemic mice induced by potassium oxonate. Methods 60 Kunming male mice were divided randomly into six groups, which were normal control group;hyperuricemic model group;and hyperuricemic groups with 20 , 40 , 60 mg/kg berge-nin, and 5 mg/kg allopurinol. Mice were orally administered once daily with 250 mg/kg potassium oxonate for 7 continuous days to create the model, and then three doses of bergenin and allopurinol were orally initiated on the day 1 h after potassium oxonate was given, separately. Serum uric acid, creatinine and urea nitrogon levels, as well as urinary uric acid and creatinine levels were measured. mRNA and protein expression levels of mouse kidney u-rate transporter 1(URAT1), and glucose transporter 9(GLUT9) were also determined. Results Compared with hyperuricemic model group, bergenin significantly reduced serum uric acid, creatinine and urea nitrogon levels, in-creased 24 h uric acid and creatinine excretion, and fractional excretion of uric acid in hyperuricemic mice;mRNA and protein levels of mURAT1 and mGLUT9 were also markedly down-regulated. Conclusion Anti-hyperuricemia effect of bergenin is attributed to the enhancement of uric acid excretion and reversion of mouse urate transporters o-ver-expression.

The integrator complex is multifunctional and contains at least 12 evolutionarily conserved subunits in hu-mans.It interacts with the C-terminal tail of the largest subunit of RNA-polymeraseⅡ ( RNAPⅡ) to promote 3′-end pro-cessing of small nuclear RNA (snRNA) U1/U2.It also interacts with RNAPⅡ, NELF and Spt5 to regulate NELF-mediated RNAPⅡpause/release and processivity at coding genes .Recently, the integrator complex is also reported to be involved in DNA damage response , dynein recruitment to the nuclear envelope , integrity of Cajal bodies , adipose differentiation , hem-atopoiesis , ciliogenesis , tumorigenesis and generation of viral microRNAs .This review discusses related research progress in the integrator complex .

BACKGROUND: Although bone tissue engineering has been developed rapidly, ideal scaffold materials are deficient and the ability of tissue engineered bone constructed in vitro was reported inconsistently.OBJECTIVE: To study the ectopic osteogenesis of the implantation in vitro with composite fully deproteinized bone(CFDB) compounded by autologous red marrow.DESIGN: A randomized controlled study.SETTING: Department of Orthopaedics, the 152 Hospital of Jinan Military Area Command of Chinese PLA; Ward for Retired Cadres, First People's Hospital of Pingdingshan City; Department of Orthopaedics, Chaochuan Mine Hospital of Pingdingshan Coal Industrial Group.MATERIALS: The study was completed in the Department of Orthopaedics,the 152 Hospital of Jinan Militatry Area Command of Chinese PLA. Totally 40 Japanese flap-eared white rabbits of 4 months old of either gender with a body mass from 2.0 kg to 2.5 kg were involved (provided by the Laboratory for Experimental Animals of the 152 Hospital in Pingdingshan city).INTERVENTION: Calf CFDB scaffold materials were compounded by rabbit autologous red marrow after physical and chemical managements, which were then implanted into the thigh muscles of 40 rabbits. The osteogenetic abilities of the materials compounded by autologous red marrow were analyzed at 4 weeks and 8 weeks after operations respectively.ysis of the implanted bone.RESULTS: ALP activities were(63.48 ± 0. 873) and (69. 527 ± 0. 635) IU/L respectively, and the results of osteogenetic quantitative analysis were (2.50 ±0.38) and(4.70 ±0.67) points of rabbits in the study group at week 4 and week 8 respectively. ALP activities were(2.50±0.38) and (4.70 ± 0. 67) IU/L and the results of osteogenetic quantitative analysis were( 1.90 ± 0.54 ) and(3.40 ± 0.54) points of rabbits in the control group at week 4 and week 8 respectively. The results indicated that the osteogenetic ability of the study group was significantly higher than that of the control group at the same time point, and the neogenetic bone increased along with the prolongation of the implantation time.CONCLUSION: CFDB could be applied as scaffold materials for bone tissue engineering, and its osteogenesis increases significantly after being compounded by autologous red marrow.

The efficacy of hematological tumor drugs has shown inter-patient variability. Pharmacogenomics focuses on gene polymorphisms of drug metabolizing enzymes, drug transporters and therapeutic targets and its impact on pharmacokinetics (PK)/pharmacodynamics (PD). Initial dose and adverse reaction can be predicted based on inherited gene polymorphisms, and therapeutic drug monitoring (TDM) also helps to adjust drug dosage in the course of treatment. Thus can achieve rational drug use and personalized medicine, and improve the efficacy and reduce the incidence of adverse drug reactions. This article will introduce the relevant research progress in recent years, and the concept of personalized medication fingerprints is proposed.

The connotations of "du-fu" and "Sanli" in "Sanli acupoint for du-fu diseases" are discussed in this paper, which can provide theoretical foundation for the clinical application of "Sanli acupoint for du-fu diseases". Based on ancient literature combined with related theories in the Huangdi Neijing (Yellow Emperor's Canon of Internal Classic), a deep discussion is performed through the relationship between Zusanli (ST 36) and stomach, indication and mechanism of Zusanli (ST 36) on du-fu diseases and comparison between Zusanli (ST 36) and Shousanli (LI 10). It is believed that "du" should be pronounced as "dŭ", meaning stomach, and it indicates that Zusanli (ST 36) is closely related to stomach and spleen when it is used for du-fu diseases; "fu" means abdomen area, including liver-gallbladder, spleen, stomach-intestine, kidney, uterus, triple energizer; "sanli' means exclusively the acupoint of Zusanli (ST 36).
Abdominal Cavity ; anatomy & histology ; Acupuncture Points ; Acupuncture Therapy ; history ; Books ; history ; China ; History, Ancient ; Humans ; Medicine in Literature ; Meridians

Objective To observe the significance of tumor abnormal protein (TAP)in the therapeutic monitoring of non-small cell lung cancer (NSCLC).Methods Peripheral blood from 30 NSCLC patients were collected to make smears at the moment of pre-treatment,half a month,one month,3 months and 6 months after therapy.TAP was detected by coacervation method.The maximum area of condense was applied to estimate the level of TAP.Thirty healthy subj ects were chose as con-trol group.Results The positive rate of TAP in NSCLC patients was 86.67%,and 3.33% of healthy subjects were positive in TAP.The difference was statistically significant (χ2=140.3,P<0.01).The condense area of TAP in patients withⅢ~Ⅳ stage of NSCLC [411(89,562)mm2]was significantly higher than those withⅠ~Ⅱ stage NSCLC [267(31,407)mm2]. The condense areas in both of two groups went down after treatment.A significant difference of condense area appeared inⅠ~Ⅱ stage of NSCLC patients a month after therapy as well as Ⅲ~Ⅳ stage of NSCLC patients half a month after treat-ment.The condense areas went to their lowest level 3 months after therapy.ForⅠ~Ⅱ stage patients,the condense area fell to 21% compared to that before treatment,but for patients withⅢ~Ⅳ stage of NSCLC,37% of pre-treatment condense ar-ea were detected.TheⅠ~Ⅱ stage of NSCLC patients had a greater decrease in condense area of TAP than theⅢ~Ⅳ stage patients by 3 months after treatment (χ2=6.22,P<0.05).Conclusion Detection of TAP in peripheral blood had a high sensitivity for NSCLC,and is able to monitoring the treatment effect.

Objective To evaluate the effects of pregnancy weight control program based on structure- process- outcome model to improve women′s health status and pregnancy outcomes. Methods On the basis of Donabedian′s structure- process- outcome quality of care model, a pregnancy weight control program designed and carried out for the middle and late pregnancy. The structure, process and outcome of the pregnancy weight control program were evaluated to ensure the quality of the pregnancy weight control program. In addition, the women′s pregnancy outcomes were investigated after intervention. Results the prenatal BMI in the intervention group [ (23.06±1.11) kg/m2 ] were lower than those in the control group [(25.52±1.89) kg/m2 ], and the incidence of cesarean section (including cesarean section without indication), neonatal macrosomia, labor block, neonatal asphyxia, postpartum hemorrhage, pregnancy induced diabetes and hypertension in the intervention group were significantly lower than those in the control group, P<0.01 or 0.05. Conclusion Donabedian′s structure- process- outcome quality of care program is an effective approach to improve women′s pregnancy outcomes.

Alternative polyadenylation ( APA) is a widespread phenomenon and an important layer of gene regulation . APA contributes to the complexity of the transcriptome by allowing a single gene to encode multiple mRNA isoforms that dif -fer either in their coding sequence or in their 3′untranslated regions (3′UTR).The length of the 3′UTR can affect the sta-bility, localization and efficiency of the messenger RNAs ( mRNAs) by altering binding sites of RNA binding proteins or mi-croRNAs(miRNAs).The polyadenylation process , mechanisms governing APA and biological consequences resulting from APA are only starting to be deciphered .Here, we review the research progress in APA .