Objective To investigate the biochemical differences between the cartilage of the residual ear of microtia patient and normal person. Methods Seven cartilage specimens from 7 about 10-years-old microtia patient's residual ears and 7 normal ears from cadaver of the same age were measured by electrophoretic mobility shift assay and spectrophotometer for collagen glycoaminoglycan (GAG), chondroitin sulfate (Chs), hyaluronan (HA) and keratin sulfate (KS) .Results Biochemical analysis showed that no significant difference were found in the collagen contents of the two groups. The microtia cartilage had more GAG (49.00?25.60) ?g/mg than that of the normal (28.25?4.80) ?g/mg. The composition of GAG were different between two groups: microtia group (HA 38.96+4.97 %, Chs 29.02 %?4.12 %, KS 32.16 %?7.41) % and normal group (HA 32.94 %?3.24 %, Chs 33.10 %?2.61%, KS 33.96 %?1.66 %). There were differences in HA and Chs relative contents, but not in KS between the two groups. Conclusions There is no difference in the collagen content between normal and microtia ear cartilage. But the latter has more GAG than the former. As for the constitution of GAG, microtia cartilage has a higher percentage of HA, lower percentage of Chs, and no significant difference in KS.

Esophageal cancer is one of the most high incidence of malignant tumors in China. However, the evaluation of chemoradiotherapy curative effect for esophageal cancer is lack of accurate and uniform criteria. In recent years, the imaging evaluation methods for chemoradiotherapy response in esophageal cancer have made some progress. The methods mainly include X-ray barium examination, endoscopic ultrasonography (EUS), computed tomography (CT), magnetic resonance imaging (MRI), positron emission tomography-computed tomography (PET-CT), and so on. Imaging examination has the advantages of safety, non-invasion and repeatability, and so on, which is a progressing tool for curative effect evaluation. Current status of the application of medical imaging which is used to evaluate esophageal cancer chemoradiotherapy curative effect were reviewed in this paper.

Along with clinical medicine improvement and imageology development, The early diagnosis and treatment of uterine cervical cancer becomes possible. Especially computer tomography(CT) and magnetic resonance imaging(MRI) as well as positron emission tomography/computed tomography(PET/CT) are widespread applied in clinical medicine. These imaging examinational means supply important bases for diagnosis, staging and therapeutic means to uterine cervical cancer.

Objective To evaluate the diagnostic value of multislice CT(MSCT)in multilocular cystic real cell carcinoma(MCRCC).Methods Imaging features of 22 patients with MCRCC proved by histopathologic examinations,these patients were underwent non-enhanced and dual-phase contrast-enhanced CT scans and evaluated CT signs.Results 22 tumors all appeared as well defined multilocular cystic mass.The cystic wall presented with irregular thicking in 14 cases and regular thicking in 8 cases.The tumors with thin coarse septum in 6 cases(the transverse diameter ≤2 mm),irregular thicking septum in 11cases(2mm ＜the transverse diameter ≤ 5 mm)and septum with nodule in 5 cases(the transverse diameter ≤ 5 mm).Cystic wall and septum enhancement were detected with dual-phase contrast-enhanced CT.Cystic ares and hemorrhagic areas and calcification were no enhanced.Calcification of cystic wall were found in 2 cases and cyst in 3 cases.Conclusion MSCT imaging appearances have typical features.It has great important value in the preoperative diagnosis of MCRCC with non-enhanced and dual-phase contrast-enhanced CT scans.

Objective To investigate the possibility of using poly-hydroxybutyate-hydroxyhexate p(3HB-co-3HH)), a modified extracellular matrix, the third generation of PHA family, as a scaffold for seeding of chondrocytes from the residual ear of microtia patients to generate tissue engineered cartilage in athymus mice. Methods The residual ear cartilage from eight 7-years-old microtia patients was enzymatically dissolved by collagenase, and chondrocytes were harvested and seeded into foams of PHB-PHH. After incubation for 1 week in vitro, chondrocyte-polymer constructs were implanted subcutaneously into 8 athymus mice. A control groups was established by subcutaneous implantation of PHB-PHH foams alone. One athymus mice were killed at 4 weeks postoperatively, and 6 were sacrificed at 8 weeks. The specimens were dissected and examined macroscopically and histologically. Results Specimens harvested from chondrocyte-polymer constructs subjected to gross morphologic and histology analysis demonstrated new cartilage formation, and those from control groups showed no cartilage formation. The one of 4 weeks still had some remains of the scaffold with nodules of neocartilage. After 8 weeks, all the 6 mice had neocartilage formed almost the same as natural. The PHB-PHH scaffold were totally absorbed. Conclusion These findings demonstrate that the foam of PHB-PHH is not only a good "matrix" for cartilage tissue engineering, but also optimal scaffold for the seeding of chondrocytes from the residual ear to generate new cartilage that would be useful in plastic and reconstructive surgery. Chondrocytes from the residual ear of microtia patients are good candidates for generation of tissue engineered cartilage.

Benign and malignant compression fracture of vertebrae are common diseases in clinic. Because their therapies are different,it is important to identify them. At present, MRI is the main means in discriminating benign and malignant compression fracture of vertebrae. Sometimes the change of MRI signal in them is overlap, therefore, it is difficult to diagnose accurately. In the recent years, with the appearance of MRI new technology, people have already applied it to work out the difficulty.

The article focuses on diagnostic criteria and diagnostic accuracy of the various imaging methods by lymph node metastasis in esophageal carcinoma at present, and compares accuracy of the various imaging methods. In general, the diagnostic accuracy of CT is lower, the diagnostic accuracy of uhrasmall superparamagnetic iron oxide-enhanced MR imaging is higher. Each imaging method has the lower accuracy for the diagnosis of smaller lymph node metastasis. Diagnostic accuracy of endoscopic ultrasonography is higher in the diagnosis of mediastinal lymph node metastasis. Sensitivity of PET/CT is higher for the diagnosis of distant lymph node metastasis.

Objective To explore the changes in secondary lesions to motor neurons caudal to a damage site on the spinal cord of rats.MethodsForty female adult Sprague-Dawley rats were given incomplete spinal cord injuries (SCIs) at the T10 level using a modification of Allen's method. Locomotor function was evaluated using an inclined plane test, modified Tarlov scores and the Basso-Beattie-Bresnahan (BBB) scale before the operation and 1,2, 3,4 and 5 weeks post surgery. Histomorphological changes at T10 and L5 were observed under a light microscope.Results①Morphology of the distal spinal cord: 1 week after SCI, neurites and Nissl bodies of the motor neurons had decreased. By the 2nd week the decrease had become more obvious. At the 3rd week after SCI, neurites and Nissl bodies had not decreased further, but neuron atrophy and apoptosis were found. At the 4th to the 5th week after SCI, neurites and Nissl bodies had increased significantly. ②Morphology of the damage site: At the 1 st week after SCI, hemorrhage and inflammation as well as nerve fiber necrosis were observed. By the 2nd week astrocytes had-increased and a few nerve fibers had grown at the damage site. At the 3rd week, regeneration of nerve fibers and astrocytes were found. At the 4th to the 5th week after SCI astrocytes and nerve fibers had not increased further. ③Locomotion function: The angles of the inclined plane test, Tarlov scores and BBB scores had decreased significantly at the 1st, 2nd, 3rd, 4th and 5th week post injury compared with those before the SCI. The values at the 2nd, 3rd, 4th and 5th week were all significantly better than those at the 1st week after SCI. There was an increasing trend in scores from the 3rd week to the 5th week, but without significant differences compared with the 2nd week after the operation.ConclusionsSecondary degeneration and damage, including decreases in neurites and Nissl bodies, neuron atrophy and apoptosis may occur in the motor neurons distal to the damage site in the early stage after SCI. This correlates with changes in the pathology of local damage.

Objective To explore the accuracy rate of CO2 injection method and conventional magnetic resonance imaging (MRI) in the staging diagnosis for stage Ⅰ carcinoma of endometrium.Methods Preoperative staging diagnosis for stage Ⅰ carcinoma of endometrium was done,and the method of injecting CO2 gas into the uterine lumen by catheter was applied for pelvic MRI scan in 38 cases of stage Ⅰ carcinoma of endometrium.The pathological staging result was treated as the gold standard to compare the accuracy rate of CO2 injection method with conventional scan method (43 cases).Results For conventional MRI scan group,the accuracy rate of staging diagnosis in stage Ⅰ carcinoma of endometrium was 81.3 ％ (35/43),including stage Ⅰ A 75.0 ％(6/8),stage Ⅰ B 83.9 ％(26/31),stage Ⅰ c 75.0 ％(3/4).For CO2 injection group,the accuracy rate was 89.4 ％(34/38),including stage Ⅰ A 85.7 ％(6/7),stage Ⅰ B 88.9 ％(16/18),stage Ⅰ c 84.6 ％ (12/13).There was a statistical difference between the accuracy rates of two methods (x2=7.81,P ＜ 0.05).Conclusion Compared with conventional scan method,the CO2 injection method with better simplicity,safety and application value,could be more accurate to determine the location of endometrial cancer and the degree of myometrial infiltration.

Objective To explore the effects of maternal allergy symptoms on the cytokine level of umbilical cord blood of neonates.Methods A cross sectional study was conducted among 136 pregnant women in Yiwu city from 1st July to 30th December,in 2012.A questionnaire on social economic charactertistics was used and blood sam-ple of pregnant women and umbilical cord blood sample were collected to detect the level of IgE,eosinophile granulo-cyte,Eotaxin,IL-9,IL-6,IL-4,IL-5,IFN-γ,IL-10.The differences between pregnant women with and without allergy symptoms were carried out.Results There were no significant differences in social and demographic characteristics between the two groups(all P>0.05).Pregnant women with allergy symptoms had higher IgE level(0.13 IU/mL vs 0.10 IU/Ml,Z=-2.063,P=0.039),eosinophile granulocyte(0.39 ×109/mL vs 0.29 ×109/mL,Z=-2.548, P=0.011),Eotaxin(66.18ng/L vs 48.35ng/L,Z=-2.144,P=0.032),IFN-γ(927.81ng/L vs 338.65ng/L,Z=-2.051,P=0.040),IL-10(15.59ng/L vs 11.55ng/L,Z=-2.022,P=0.043) than pregnant women without allergy symptoms in neonates′cord blood.Conclusion Maternal allergy symptoms may increase the level of IgE, eosinophile granulocyte,Eotaxin,IFN-γand IL-10 of neonates′cord blood.