Objective:To investigate the expression and significance of ABCG2(the G2 member of ATP-binding cassette family)in U251and its stem cell.Methods:The U251 were cultured in serum-free medium with EGF,bFGF,LIF and B27.The tumor spheres formed and cultured continuously in the serum-free medium through the monoclonal formation experiment.The immunofluorescence staining was employed to identify the U251 stem cell.The expression of ABCG2 was detected by confocal microscopy and Western blot.Results:The stem cells from malignant glioma cell line U251 have been successfully isolated.The cells expressed CD 133.The ABCG2 protein positive stain was located in the cell membrane in both U251 cells and its stem cell with confocal microscopy method.Western blotting showABCG2 was expressed in U251 cells and in its stem cells.The expression level in the stem cells was significantly higher than U251 cells.Conclusion:ABCG2 is expressed in U251 cells and in U251 stem cells.The expression level in the latter was higher.

To investigate the relationship between hyperglycemia and hyperlipidemia in diabetics, eighty-six patients with non-insulin dependent diabetes mellitus (NIDDM) were followed up for 6 to 12 months. The results showed that plasma TC, TG, LDL-C levels and LDL-C/HDL-C ratio were elevated in diabetic group as compared with normal control group. Plasma TC and LDL-C levels in the diabetics with mean fasting blood glucose (mFBG) level more than 150 mg/dl were significantly higher than those in diabetics with mFBG less than 150 mg/dl, and those in normal control group as well, but plasma TC, LDL-C and TG levels showed no significant difference between diabetics with mFBG less than 150 mg/ dl and normal controls.The disturbances of lipid metabolism were obviously alleviated following the improvement of diabetic control. These results suggest that better diabetic control can be beneficial to the improvement of lipid metabolic disorders and might be helpful to the prevention of angiopathy in NIDDM patients.

In the past five thousand years,the advanced theory and effective practice of traditional Chinese medicine have played a key role in the prosperity and population growth of traditional Chinese people.This article analyzed the opportunities and challenges that traditional Chinese medicine faces in its current state of development and discussed the progressiveness of traditional Chinese medicine theory and practices.The author advocates that "the essential task of developing traditional Chinese medicine is to preserve and expand the progressiveness of traditional Chinese medicine theory and practices".In the 21 century,human health concept and medicine model have been changing.And,the fast pace development in modern technology and our country's growing emphasis in traditional Chinese medicine offer a great opportunity for traditional Chinese medicine to evolve.In such a juncture,the author believes that the key to the further development of traditional Chinese medicine rests in the correct understanding,preservation and expansion of the advanced traditional Chinese medicine theory and practices.To do so,we need to focus on "fundamental development" and "independence innovation" aiming at using traditional Chinese medicine to continuous improving our capability not only in disease prevention and treatment but also in science and technology innovation to allow traditional Chinese medicine to make more contribution in protecting human health.

The relationship between plasma magnesium and plasma lipids was studied in 80 cases of Type II diabetes mellitus. It was found that plasma magnesium in diabetic patients was much lower than that in normal controls. Plasma magnesium was negatively correlated with plasma cholesterol, triglyceride, and LDL-C, and positively correlated with plasma HDL-C, HDL2, and HDL-C/LDL-C, suggesting that hypom-agnesemia was closely related with disturbance in lipid metabolism in diabetes mellitus. Hypomagnesemia seemed to worsen the disturbance of lipid metabolism, and might be one of the factors causing vascular complications in diabetes mellitus

OBJECTIVE:To compare the clinical efficacy and safety of Shexiang baoxin pills and Shensong yangsin capsules in the treatment of coronary heart disease complicated with chronic heart failure and premature ventricular contractions. METHODS:129 patients with chronic heart failure and premature ventricular contractions were randomly divided into blank group(n=43),ob-servation group(n=43)and control group(n=43). All groups received routine treatment as oxygen inhalation,diuretics. Control group additionally received Shensong yangsin capsules 4 pills for 3 times per day. Observation group additionally received Shexiang baoxin pills 2 pills for 3 times per day. Treatment course of 2 groups lasted for 3 months. Clinical efficacies of 2 groups were ob-served,and heart function,cardiovascular autonomic nervous function and vascular endothelial function were observed before and after treatment. RESULTS:Total effective rate of observation group was significantly higher than that of control group(P<0.05). SDNN,SDANN,RMSSD,QTd was no statistical significance between control group and observation group(P>0.05). LVEF, NO of observation group were significantly higher than that of control group(P<0.05),IVST,LVDd,LVPWT,ET of observation group were significantly lower than that of control group(P<0.05). But control group and observation group were better than the blank group,and no obvious adverse reaction. CONCLUSIONS:Shexiang baoxin pills is more effective than Shensong yangsin cap-sules in the treatment of coronary heart disease complicated with chronic heart failure and premature ventricular contractions.

OBJECTIVE:To observe the clinical efficacy and safety of Mailuoning injection in the treatment of acute bronchial asthma. METHODS:92 patients with acute bronchial asthma were randomly divided into control group and observation group. Pa-tients in control groups were received routine treatment,including oxygen inhalation,glucocorticoid,β2 agonists,aminophylline and anti-infection,etc. Patients in observation group were added Mailuoning injection 20 ml adding into 5% glucose injection or 0.9% sodium chloride injection 500 ml by intravenous infusion,once a day,based on the treatment of control group. The course was 14 d. The clinical data was observed,including clinical efficacy,hemorheology indicators,pulmonary function,6-keto-PGF1αlevel and incidence of adverse reactions before and after treatment. RESULTS:The total effective rate in observation group was sig-nificantly higher than control group,with significant difference(P<0.05). After treatment,the hemorheology indicators,lung func-tion indicators and 6-keto-PGF1α level in 2 groups were significantly better than before,and observation group was better than con-trol group,with significant difference(P<0.05). There were no obvious adverse reactions. CONCLUSIONS:Routine treatment combined with Mailuoning injection can effectively improve the efficacy of acute bronchial asthma with good safety.

Cerebral ischemia is one of the diseases of the central nervous system, which is harmful to the morphology and function of brain cells due to reduced cerebral blood flow. Increasingly more reports have demonstrated that spinal cord stimulation could augment the cerebral blood flow, which is expected to be a potential method for the treatment of cerebral ischemia. In this article, the research progress and related problems of spinal cord stimulation for the treatment of cerebral ischemia are reviewed.

Objective To understand the use of traditional Chinese medicine in treating osteoporosis in South Korea's academic institutions during the past ten years.Methods Database of South Korean academic papers on the treatment ofosteoporosis with traditional Chinese medicine from 2003 to 2012 were searched,and 56 academic papers were retrieved.Results Of 34 selected research papers on single herb,20 papers were on herb with tonifying function,accounting for 58.8％; 4 papers were on herb with invigorating blood and dispersing blood stasis function,accounting for 11.8％; and 10 papers on herb with tonifing kidney Yang function,accounting for the remaining 29.4％.The other 22 papers were on Chinese herbal formulas.Among them,16 papers were on formulas with tonifiying function,accounting for 72.7％.Of all 17 Youguiyin dominate formulas,14 formulas showed main functions of supplementing Kidney,accounting for 82.4％.Conclusion In the osteoporosis research papers using single herb and Chinese formulas,the ones with the function of supplementing,especially supplementing kidney were chosen by a high ratio.

The activity of erythrocyte membrane Na-pumping K~+-activated pera-nitro-phenylphosphatase (K~+-pNPPase) and red cell deformability (RCD) were stndied in 50adult patients with non-insulin-dependent diabetes mellitus (NIDDM). RCD was expressedas red blood cell filtration index (RBC-IF). In comparison with the healthy subject, theactivity of erythrocyte membrane Na-pumping K~+-pNPPase was significantly decreased,and RBC-IF was significantly increased. The above-mentioned changes were more severein NIDDM with retinopathy than those without. There was a very close negative corre-lation between Na-pumping K~+-pNPPase and RBC-IF. These results indicated that thedecrease of erythrocyte membrane Na-pumping K~+-pNPPase activity might be an importantfactor fot the decrease of RCD.

Five hundred and ninety-one papers retrieved from CAJD on performance assessment of electronic re-sources in domestic libraries from 2004 to 2013 were retrospectively analyzed by biliometrics, which showed that these papers focused on the description of projects,methods of assessment,and establishment of assessment indexes. The following problems were pointed out, such as weakness in research strength, tendency to stress theories, inopera-tive methods used in studies, and single-valued achievements, with suggestions put forward for their solution such as intensifying cooperation and focusing on practice in future studies.