Objective To analyze the correlation between five cases of female vaginal infection based on the vaginal discharge routine (morphology) and enzyme spectrum.Methods 1 000 patients with vaginal secretion examination were selected as the research subjects of this study.Included patients were treated by routine examination of Trichomonas vaginalis and epithelial cells, white blood cells and Candida, combined pre vaginal secretion of enzymes in the five test.Results The combination of the five enzymes of vaginal secretion examination found in 1 000 patients, the positive patients were 620 cases, accounted for 62.0% of the total, vaginal secretion was more common for aerobic bacteria (AV), accounted for 59.2%.AV combined with aerobic bacteria Candida (VVC) and bacterial vaginosis (BV) was one of the main pathogenic bacteria;data showed that the total positive rate of H2O2 accounted for 73.8%.Conclusion Based on the combination of the five enzymes and the leucorrhea routine examination of vaginal secretions of female vaginitis patients can effectively reflect the specific state of patients with vaginal infection,and it is conducive to the clinical treatment of late symptomatic.

Objective To explore predictive effects of organism weak magnetic field analytic technology on heart diseases among adults with hypertension,hyperglycemia and hyperlipidemia.Method A total of 1534 individuals who received health examinations and organism weak magnetic field tests in Health Science Center of Chinese PLA General Hospital from May to June 2009 were enrolled.Statistical analysis were carried out to detect if the heart function index of organism weak magnetic field tests could identify different conditon for the incident dysfunction of heart in low-,potential-,high-risk groups classified by the three high agent using the nonparametric test.High-dangerous three-high groups include simple,compound,additional and assembling sorts of combining types.Results The quantitative values of heart meridian of one potential-risk and nine high-risk three-high groups differs evidently from the low-risk high-risk three-high groups by Moses test(P ＜ 0.05).There was statistically significant difference of the quantitative values of heart meridian,potassium,magnesium,manganese,cardiovascular,circulaton,heart endocrine,and T2 among the low-,potential-and high-risk levels and other different types of three-high groups by Wilcoxon rank sum test(P ＜ 0.05).Conclusion Organism weak magnetic field analytic technology could reveal change of heart dysfunction in individuals with normal electrocardiogram.

Objective To explore the predicting role of organism weak magnetic field analytic technology on heart functions in individuals with normal electrocardiogram(ECG).MethodsA total of 1534 subjects who receired health examinations and organism weak magnetic field tests in Health Science Center of Chinese PLA General Hospital from May to June 2009 were enrolled.Positive rates of organism weak magnetic field analytic technology,echocardiography,and myocardium enzyme test for heart dysfunction among populations with hypertension,hyperglycemia and hyperlipidemia were compared.Statistical analysis was carried out to detect if there was close correlation between different heart risk and the weak magnetic field index.Results(1)The positive rate of the organism weak magnetic field analytic technology for heart dysfunction among subjects with normal ECG was 96.0%(1472/1534),which was significantly higher than echocardiography(27.5%,200/726)and myocardium enzyme testing(6.9%,106/1532).The positive rate of organism weak magnetic field analytic technology was increased with the seriousness of hypertension ,hypergjycemia and hyperlipidemia(P＜0.05＝.(2)Different heart risks showed close correlation with heart meridian and potassium(P＜0.05＝.Conclusion The prediction of organism weak magnetic field analytic technology on potential heart dysfunction(as a result fromhypertension,hylperglycemia and hyporlipidemia)in individuals with normal ECG is highly sensitive andrelatively objective.

Objective To compare the influence of early weight bearing exercise and plaster fixed method on postoperative function and fracture healing of unstable ankle fracture patients.Methods 50 cases of unstable ankle fracture patients who did surgical treatment in our hospital were selected as the research object,and were randomly divided into an observation group and a control group.The observation group was applied with early postoperative weight bearing exercise,while the control group patients was applied with postoperative plaster fix with 2 weeks.The postoperative function and fracture healing situation of both groups were compared.Results At postoperative 24 hours and 1 week,the perimeter difference of two groups were not statistically different (P＞0.05).At postoperative 6 weeks,the perimeter difference was (0.53 ±0.31) cm in the observation group and (1.25±0.47)cm in the control group,the comparative differences was statistical significance (t=6.39,P＜0.05).The total effective rate of alleviate swelling in the observation group at postoperative 6 weeks was 96.0％,which was higher than that of the control group (76.0％) with statistical difference (x2=4.15,P＜0.05).At postoperative 24 hours,postoperative 1 week and postoperative 6 weeks,the pain index comparison difference between the two groups was not statistically significant (P＞0.05).The Baird-Jackson score of the observation group at postoperative 3 months was not different with the control group with statistically significant (P＞0.05) ; At the postoperative 6 months,the Baird-Jackson score of the observation group was (95.21 ± 8.25),which was higher than that in the control group (82.52±6.96) with statistical difference (t=5.88,P＜0.05).Conclusion The early weight bearing exercise can promote haemal circumfluence,relieve swelling,and be helpful for function recovery and fracture healing of unstable ankle fracture patients.

We carried out a new unit-based method in the teaching process of epidemiology for improving undergraduate prevention-oriented medical students' comprehensive ability. The new method integrated heuristic teaching and case teaching, and focused reform on the system of teaching and assessment. The results showed new teaching method can significantly improve students' capacity of analysis and oral expression, and contributes to the comprehensive ability.

AIM: In order to study the relationship between the ERK and p38 MAPK activation and the protection of 11, 12-epoxyeicosatrienoic acid (11, 12-EET) and ischemia preconditioning (IP), the effects of 11, 12-EET and ischemic preconditioning on phosphorylated ERK and p38 MAPK during ischemia and reperfusion in rat myocardium were examined. METHODS: The rat heart was subjected to ischemia for 5 min by ligating the left anterior descending coronary artery followed by reperfusion for 5 min (two times) to undergo ischemia preconditioning. The rats were divided into 5 groups: (1) control; (2) sham group; (3) ischemia/reperfusion (I/R) group, in which the rat heart suffered from 60 min ischemia followed by 30 min reperfusion; (4) IP plus I/R group; (5) EET plus I/R group, in which 6.28?10 -8 mol/L 11, 12-EET was injected intravenously 20 min before I/R. The heart function was examined, and phosphorylated ERK and p38 MAPK were detected by Western blot. RESULTS: At 30 min reperfusion, +dp/dt max ,-dp/dt max and LVDP decreased significantly in I/R group compared with sham group, IP plus I/R group and EET plus I/R group; Phosphorylated ERK1/2 level was higher in I/R group than sham group, but was lower in I/R group than IP plus I/R group and EET plus I/R group; Phosphorylated p38 MAPK level was lower in control, sham, IP plus I/R and EET plus I/R group than I/R group. CONCLUSION: 11,12-EET protects rat heart against ischemia/reperfusion injury, the mechanism may be related to activation of ERK1/2 and inhibition of p38 MAPK. [

Objective To investigate the effect of general nursing intervention on the mental state of the patients with breast cancer undergoing chemotherapy.Methods Forty-eight breast cancer patients undergoing postoperative chemotherapy received nursing general interventions including healthy education,psychological nursing care,pantosomatous,relaxation therapy and rehabilitative exercise. All patients were evaluated by self-rating depression scale (SDS) and depression status inventory (DSI) before and after the general intervention.Result The scores of SDS and DSI after intervention were significantly lower than those before intervention (bothP<0.05).Conclusion The general nursing intervention could significantly improve patients’ psychological state and life quality,promote rehabilitation and improve their quality of life.

Humans ; Leukoplakia, Oral ; diagnosis ; Mouth Diseases ; diagnosis

Objective:To study the optimal pain control goal for preventing delirium in critical patients.Methods:A prospective cohort study were conducted. The patients admitted to general departments and transferred to the intensive care unit (ICU) due to critical illness in the First People's Hospital of Changde from January 2017 to November 2019 were enrolled. The General data of the patients were collected within 48 hours after admission. All patients admitted to the ICU were evaluated for pain level using the critical care pain observation tool (CPOT) every 8 hours by nurses, and confusion assessment method of ICU (CAM-ICU) was used to screen delirium patient every 8 hours by the leader of nursing team without knowing the pain level of the patients, until the subjects were transferred out of ICU. The receiver operating characteristic (ROC) curve was drawn, the area under ROC curve (AUC) and the optimal threshold were analyzed with delirium as the reference standard; according to the optimal threshold, multivariate Logistic regression analysis was used to evaluate the correlation between CPOT score and delirium.Results:During the study period, 575 patients were admitted to the participating departments and passed the preliminary screening according to the inclusion and exclusion criteria. During the study period, 34 patients were excluded due to incomplete data. Finally, a total of 541 patients were enrolled in the analysis, including 149 patients in delirium group and 392 patients in non-delirium group. There was no significant difference in gender, age, source of patients, education level, smoking history, drinking history, family mental history, acute physiology and chronic health evaluation Ⅱ (APACHEⅡ) score or other general information between the two groups. There were 10.1% (15/149) of patients in the delirium group used opioids, which was significantly higher than 4.3% (17/392) in the non-delirium group, and the difference was statistically significant ( P < 0.05). The CPOT score in the delirium group was significantly higher than that in the non-delirium group (4.24±1.78 vs. 2.75±1.95, P < 0.01). The patients were subdivided into young group (< 40 years old), middle-aged group (40-65 years old) and old group (> 65 years old) according to age. The analysis results were consistent with the overall analysis results. ROC curve analysis showed that the AUC of CPOT score predicting delirium was 0.719; when the best threshold value of CPOT score was 2.5, the sensitivity was 91.3%, the specificity was 49.0%, the positive predictive value was 40.5% and the negative predictive value was 93.7%. Multivariate Logistic regression analysis showed that the risk of delirium in ICU patients with CPOT score ≥ 3 was 10.043 times higher than that in patients with CPOT score < 3 [odds ratio ( OR) = 10.043, 95% confidence interval (95% CI) was 5.498-18.345, P < 0.001]. When the gender, age, APACHEⅡ score, smoking history, drinking history, opioids usage were adjusted, the risk of delirium in patients with CPOT score ≥ 3 was 10.719 times higher than that in patients with CPOT score < 3 ( OR = 10.719, 95% CI was 5.689-20.196, P < 0.001). Conclusion:The best pain control goal for preventing the occurrence of delirium in ICU patients is a CPOT score of 3 or less.

It was introduced in this paper the concrete measures of medical humanistic education which is in harmony with the teaching of human parasitology. The necessity of humanistic education for the medical students in the present age and the feasibility of humanistic education in basic medical education were discussed according to the feedback on the survey of the medical students. This study aimed to explore an ideal teaching model for medical humanistic education and comprehensively improve the humanistic accomplishment in medical students.