Objective To explore the status of obesity and diet behavior in young and middle-aged people of Jinan and supply reference for controlling overweight and obesity by diet intervention. Methods Stratified sampling method was conducted to investigate 18～59 inhabitants in Jinan. The information about their body height,body weight and diet behavior was collected to further analyze the relationship between the status of obesity and correlated behaviors by the SPSS 11.5 software. Results The prevalence rate of overweight and obesity had a significant difference between young group and middle-aged group(P＜0.01), which was separately 21.56% and 33.46%; the prevalence rate of overweight and obesity in young group had a significant difference between males and females(P＜0.05),but no significant difference in middle-aged group.Compared with the diet behavior problems of normal body weight people, the incidence rate of overweight and obesity was significantly higher(P＜0.01),but no significant difference for the habit of midnight snack.Females paid more attention to reasonable match and the speed of food intake than males.Conclusion Overweight and obesity in young and middle-aged people of Jinan is relatively serious,which is closely correlated with unscientific diet behavior.

Objective To assess the prevalence and impacts of premenstrual syndrome on female college students,and to provide scientific basis for health education. Methods In the cross-sectional study, a total of 900 female college students from three universities in Jinzhou city were investigated.The data were compiled using the questionnaire of Premenstrual Syndrome Scale by John Bancroft and the general information questionnaire.Results A total of 856 questionnaires were valid among the 900,the validity rate was 95.11%. Premenstrual syndrome was diagnosed in 32.7%(280/856)of all cases,distributed as 22.7%(194/856)mild,7.9%(68/856)moderate and 2.1%(18/856)severe. Binary Logistic regression analysis revealed that premenstrual syndrome was significantly associated with bad sleep condition, animal fat intake, yolk food intake, dysmenorrhea, early age at menarche. Conclusions The results showed that premenstrual syndrome is prevalent among female college students and that life style and nutritional factors may play an important role in this disorder. Therefore,health education and counseling services,as part of the overall health service,should be availed and provided to affected women.

Objective To discuss the efficacy and safety of decitabine plus aclacinomycin/cytarabine/ G-CSF (CAG) regimen in the treatment of patients with relapsed refractory acute myeloid leukemia (AML).Methods 17 cases with relapsed refractory AML were collected.They were given decitabine (25 mg/d,d1-5) plus CAG regimen (aclacinomycin 20 mg/d,d3-6; cytarabine 10 mg/m2,d3-9; G-CSF 400 μg/d,d0-9).Results After one course treatment by decitabine plus CAG regimen,10 patients (58.8 ％) achieved complete response (CR),1 patient (5.9 ％) achieved partial response (PR) and 6 patients (35.3 ％) displayed no response (NR),the overall response rate was 64.7 ％ (11/17).For karyotyping of chromosomal abnormalities,only 1 case (100 ％) in CR showed normal karyotype.8 cases (66.7 ％) in CR,1 case (8.3 ％) in PR showed medium karyotype.1 case (25.0 ％) in CR showed bad karyotype.Statistical analysis revealed that there were significant differences among different groups (P ＜ 0.05).Follow-up to January 31,2013,15 patients survived,2 patients died early.The median overall survival was 67 d (14-307 d),and the median relapse-free survival was 47 d (14-152 d).Conclusions The combined regimen of decitabine and CAG in the treatment of patients with relapsed refractory AML has a high response rate,little non-hematologic side effects and good tolerance.

Cloud point extraction(CPE) utilizing a cationic surfactant, tricaprylmethylammonium chloride(Aliquat 336), was carried out to separate and concentrate bisphenol A(BPA) from aqueous media. Cloud point phase separation of Aliquat 336 at room temperature(about 25 ℃) was induced by the addition of Na_2SO_4. The parameters affecting extraction efficiency, such as concentrations of Aliquat-336 and Na_2SO_4, pH, and equilibration time were evaluated. The results showed that the extraction efficiency was practically constant in the studied range of Aliquat 336, co-solvent(methanol), pH and dissolved organic matters. At the operational CPE conditions, distribution constant, K_D, of the distribution of BPA between the surfactant-rich and aqueous phases of the CPE system was estimated to be 10~4. Coupled to liquid chromatography with UV detection, the CPE method offered a detection limit of 0.34 μg/L in distilled water and recovery of BPA ranged from 90% to 108.6% with a sample volume of 50 mL in three matrices studied. The high extraction efficiency might be due to combination of ion-pair, hydrophobic effects and cation-π interaction, based on the micelles' size distribution and zeta potential analysis.

Objective To learn the relationship between working pressure and mental health for nurse of transfusion room in pediatric department.Methods 120 nurses of transfusion room whose working years above half a year were investigated by questionnaire from January 2010 to December 2011.Results The maximal score of working pressure was working environment and resource (3.27±0.47),while the minimum was interpersonal relationship (1.80±0.68).The score of SCL-90 of the research group was higher than domestic model.More working pressures of transfusion room in pediatric department were positive correlated with SCL-90.Conclnsions In order to keep nurse sound in body and mind,the working pressure should be desolved in many ways.

Osteoarthritis (OA) is a kind of chronic and frequently-occurring disease in the elderly.It is also called degenerative disease.Pain,stiffness,swelling,deformity and dysfunction of joints are the main clinical manifestations,which seriously affect the quality of life.Kaschin-Beck Disease (KBD) is a kind of endemic diseases;its main feature is cartilage degeneration and necrosis.Although KBD becomes a typical OA in late stage,it is different from OA.Osteoprotegerin (OPG) and insulin-like growth factor (IGF)-1 participate in the bone growth,repair,and alteration.In this paper,we summarized clinical manifestations and pathological changes of OA,and reviewed the current situation and development of OPG and IGF-1 in OA bone remodeling.

cases of acute promyelocytic leukemia (APL) were treated with drugs of all trans retinoic acid (ATRA) and Proplylene glucol mannurate sulfate (PGMS).Complete Remission rate was 90%. Because of using Proplylene glucol mannurate sulfate (PGMS), the rate of DIC and the rate of death were decreased.

Objective To assess the efficacy of diffusion weighted imaging( DWI) with Magnetic Reso-nance Imaging to predict the T-staging in rectal cancer.Methods One hundred and seven cases confirmed by pathology of rectal adenocarcinoma patients were retrospectively analyzed.T-staging was determined by MRI and DWI combined with MRI.All the patients were underwent surgery within one week after MRI.We compared the results with the pathological T-staging after surgery,and compared the two methods of the preoperative T-stag-ing rectal cancer diagnosis accuracy.Results The accurate rates for T-staging of rectal cancer using MRI was 84.11%(90/107) .There was a correlation between the result of preoperative MRI and postoperative pathological T-staging(kappa=0.652,P<0.001);The accurate rates for T-staging of rectal cancer using combined MRI with DWI was 90.65%(97/107).There was a better correlation between the result of MRI with DWI and postop-erative pathological T-staging(kappa=0.732,P<0.001).Conclusion Compared with the conventional MRI sequences,the accuracy of MRI combined with DWI for diagnosis of T-staging in rectal carcinoma is higher.It can provide a more reasonable treatment.

OBJECTIVE:To investigate the effect of arsenic trioxide(As2O3)on human hepatoma cells SMMC-7721 viability and the expression of urokinase plasminogen activator receptor(uPAR).METHODS:SMMC-7721 cells were cultured and treated with As2O3 at different concentrations for different time with untreated SMMC-7721 cells(without As2O3 treatment)served as control.The proliferation rate of the SMMC-7721 cells was measured by MTT assay;the change of cell cycle was detected by flow cytometry analysis;the effect of As2O3 on uPAR mRNA levels was analyzed by real-time fluorescent quantitative polymerase chain reaction.RESULTS:As2O3 had significant inhibitory effect on SMMC-7721 proliferation in time-dependent and concentration-dependent manner.As compared with control group,As2O3 significantly decreased the proportion of cells in G0/G1 phase but increased the cell proportion in G2/M phase,and it markedly down-regulated the level of uPAR mRNA expression(P

Objective: To investigate the antiatherosclerotic effect of the extract from grape seed in C57BL/6J mice. Method: The mice were fed with atherosclerotic diet for 20 w and then with basic diet or different dosage of grape seed extract for 4 w. The blood samples were examined for blood lipid. The sections of aorta were examined and scored microscopically. Results: After fed with atherogenic diet for 20 w, the aortic score was 7.2?1.3 and TG, Chol and MDA in serum were significantly increased. After fed with the extract of grape seed for 4 w, the aortic score was reduced to 6.0?1.2, and TG, TC, and MDA levels also decreased. Conclusion: The extract of grape seed has antiatherosclerotic effect.