BACKGROUND: Cryoimmunology has been paid more attention in recent years, but the effect of cryoablation on cellular function has not been clearly understood, especially for the cellular apoptosis and dendritic cells.OBJECTIVE: To evaluate whether NCI-H446 lung cancer cells could activate bone marrow-derived dendritic cells (DCs) which stimulate lymphocytes to be cytotoxic lymphocytes (CTLs) to kill cancer cells?DESIGN, TIME AND SETTING: Cellular immunology experiment was performed at the Hematological Laboratory, Beijing Naval General Hospital, and Genetics Center of Military Medical Science Academy of the PLA between August 2002 and April 2003.MATERIALS: NCI-H446 cells were small lung cancer cells purchased from Shanghai Institute of Sciences.METHODS: Cultured lung cancer cells were cryo-treated and added to cultured DCs in the presence of naive lung cancer cells. Morphological changes of cancer cells were observed; mixed lymphocyte reaction (MLR) and antitumor activity of cytotoxic T lymphocyte (CTLs) were also evaluated.MAIN OUTCOME MEASURES: Morphology of DCs and cell surface immunophenotype changes; MLR of cultured cells and cell apoptosis.RESULTS: ① of 51 patients,18 were followed-up for 6-12 months,2 for 13-18 months,and 8 for 19-24 months.② A total of 49 (96.1%) out of 51 patients preserved the eyeballs and the visual acuity improved to different degrees in 48 (94.1%) patients.③ After surgery,fungal infection recurred in 6 eyes (11.6%),4 of which were controlled by antifungal medication and 2 was enucleated because of uncontrolled endophthalmitis.Graft rejection was found in 18 (35.3%) eyes, 13 of which recovered transparent by medication and 5 received secondary PKP.Graft ulceration was present in 4 (7.84%) eyes, 3 of which were cured and the remaining one was re-grafted because of severe endothelial cell loss. Secondary glaucoma appeared in 7(13.7%) eyes,and the intraocular pressure was controlled medically and surgically.Complicated cataract occurred in 6 (11.8%) eyes,3 of which underwent cataract extraction. Most complications were successfully controlled.In the final follow-up period,45 (88.2%) grafts were transparent.CONCLUSION: Cryo-treated lung cancer cells exhibited cell lysis and membrane collapse, which activate bone marrow-derived DCs to kill cancer cells specifically.

Objective To compare the clinical efficacy of laparoscopic and open method in the treatment of gastric cancer radical surgery.Methods 1 1 0 patients who underwent radical surgery for gastric cancer were selected. According to its different operation method,they were divided into observation group and control group,55 cases in each group.The observation group received laparoscopic surgery,and the control group received open operation treat-ment.The general surgery situation,the C -reactive protein (CRP)levels at preoperation and postoperative 1 ,7,1 0d were compared in the two groups.Results The surgical incision length,intraoperative blood loss,postoperative bowel movements and postoperative hospital stay in the observation group were (24.6 ±3.7)cm,(99.9 ±31 .8)mL,(5.8 ± 2.7)d,(1 2.8 ±7.7)d respectively,which were significantly lower than (5.6 ±1 .4)cm,(61 .3 ±1 3.6)mL,(4.5 ± 0.7)d,(9.2 ±3.9)d in the control group(t =31 .472,5.289,3.1 94,2.398,all P <0.05),the number of the lymph node in the two groups had no significant difference(P >0.05).CRP level at 1 d preoperation,1 d postoperation,7d postoperation,1 0d postoperation of the control group were (1 8.21 ±3.67)mg/L,(42.96 ±7.28)mg/L,(22.88 ± 4.35)mg/L,(15.89 ±4.34)mg/L respectively,which of the observation group were (17.82 ±3.57)mg/L,(29.97 ± 4.58)mg/L,(1 6.01 ±3.1 6)mg/L,(8.65 ±1 .77)mg/L respectively,it had no significant difference between the two groups at preoperative 1 d (P >0.05 ),postoperative 1 d both elevated obviously,postoperative 7 d,1 0 d both significantly dropped than postoperative 1 d,and the differences between the two groups were significant (t =7.497, 6.521 ,8.339,all P <0.05).The incidence rate of complications was 5.45%(3 /55)in the observation group,which of the control group was 1 8.1 8%(1 0 /55),the difference between the two groups was statistically significant(χ2 =6.371 ,P <0.05).Conclusion Compared with the traditional laparotomy,laparoscopic gastric cancer radical has mild trauma,rapid recovery,the effect is good,quite cure degree,which is worthy of clinical popularization and application.

This article based on the experience of LIN Lan’s clinic and scientifi c research,analysis the etiological factor and pathogenesis of diabetes mellitus(DM)complicating coronary heart disease(CHD),presumes that defi ciency of both qi and yin,blood stasis and sputum are mainly pathogenesis of DM complicating CHD.Professer LIN proposes the TCM treatment based on syndrome differentiation,which obtains obviously therapeutic effect.

As the economic reformation goes on, the traditional culture and ethics anr chanllenged. Should we carry on the spirit of Betheune? The hospital, cultural development focuses on how to carry forward the spirit of Betheune. Acording to his experience, the author explained his view and put several points that we should notice in new situation. 1. Emancipate the mind and change the view; 2. Advocate the unselfish contribution combined with the policy of contribution considering oneself; 3. Requine the level of conbined with regulatiton.

Some of the clinical manifestation of primary immunodeficiency disease in children is similar with that of hematological system diseases in children.It would result into the change of one or multiple lines of blood cells, which would lead to infection or malignant tumors.To strengthen the awareness of the hematologic manifestations of immunodeficiency diseases will be beneficial for doctors in the diagnosis of diseases,judgment of complication and prog-nosis.

Objective:To compare the effect of curing polymyalgia rheumatica either by using both Biqi capsule and prednisone or by using prednisone alone. Methods: Thirty-nine patients of polymyalgia rheumatica were randomly divided into two groups, 18 patients in the treatment group treated with Biqi capsule and small dosage of prednisone; 21 patients in the control group treated with small dosage of prednisone. The therapeutic course was 12 weeks. The patients' symptoms of muscle ache, inflexibility in the morning and T-cell subsets determination (CD4, CD8)were observed and scores of clinical practice was made. Results: The respective total effective rate was 94.44% for the treatment group and 66.67% for the control group. There was distinctive difference in the scores for the treatment group and the control group in relieving the patients of the symptoms of polymyalgia rheumatica. The treatment group would help the patients of polymyalgia rheumatica to improve their immunity ability. Conclusion: The combination of Biqi capsule and prednisone can help to improve the effect of curing polymyalgia rheumatica.

Objective To evaluate the safety and efficacy of microsurgical resection of large acoustic neurinoma via the suboccipital retrosigmoid approach.Methods Forty-nine patients with large acoustic neurinoma(≥4 cm) underwent microsurgical resection through suboccipital retrosigmoid approach.The craniotomy was performed by way of an unilateral S-shaped suboccipital incision.With microsurgical techniques the outmost layer of the arachnoid membrane was preserved in order to avoid damaging to the surrounding vital structures.The tumor was resected intracapsularly from the superior pole and the internal auditory meatus was finally opened.The last pieces of tumor were removed by sharp dissection from the facial nerve bidirectionally,and were resected cautiously in a piecemeal fashion.Results Of the 49 patients,45 patients(92%) received a total resection of the tumors,and 4 patients(8%) subtotal resection.No patients died.The facial nerve was preserved anatomically in 42 patients(86%) and functionally in 36 patients(73%).The acoustic nerve was preserved anatomically in 7 patients(14%) and functionally in 3 patients(6%).One patient experienced a postoperative haematoma,and a re-operation was required.No recurrence was seen in 37 patients during a follow-up for 6 months ～ 5 years(mean,2.8 years).These patients had recovered for normal work and daily activities.Conclusions Microsurgical operation through suboccipital retrosigmoid approach is a favorable treatment for large acoustic neurinomas.The procedure can improve the rate of total resection,decrease the morbidity and mortality,and effectively protect the function of the acoustic and facial nerves.

Objective To compare the effects of different doses of sufentanil on hemodynamic response to anesthesia induction in patients with hypertension undergoing coronary artery bypass graft (CABG). Methods 45 ASAⅡor Ⅲ patients aged 46~65years old and weighing 58~79kg scheduled for elective undergoing CABG surgery under general anesthesia requiring tracheal intubation were randomly divided into 3 groups(n=15 each)according to different doses of sufentanil. The patients were premedicated with intravenous scopolamine 0.005mg/kg and midazolam 0.05mg/kg. Anesthesia was induced with etomidate 0.3mg/kg and rocuronium 0.6 mg/kg and then received 1.0?g/kg(group S1) or 1.2?g/kg (group S2) or 1.5?g/kg (group S3) of sufentanil injected intravenously slow-moving at 3 min before orotracheal intubation. ECG,HR,BIS,BP and SpO2 were monitored and recorded before induction of anesthesia(the baseline values T0),and after induction of anesthesia and before intratracheal intubation instantly (T1),1 min(T2),5 min (T3)and 10 min (T4) after intratracheal intubation.Results There were no significant differences between 3 groups with respect to age,sex,body weight and hypertension class. Hemodynamic variables were significantly changes at 3 groups at T1-4 than T0(P

Objective: To observe the therapeutical effect of intermittent positive pressure ventilation (IPPV) on pulmonary edema after seawater drowning in rabbits.Methods:Fourteen seawater lavage induced pulmonary edema New Zealand rabbits models were assigned to receive IPPV treatment.Blood gas analysis, pulmodynamics, hemodynamics status and serum tumor necrosis factor(TNF ?) were monitored at various time points.After 3 h ventilation rabbits of each group were killed to gain lung tissues for pathology examination, and lung lavage fluid was analyzed to count leukocytes.Results:PaO 2 significantly increased after the use of ventilation in pulmonary edema rabbits models ( P

Perioperative blood transfusion therapy for coagulation factor deficiency in children is very important to save patient's life or conducive to the postoperative recovery.With the advances in transfusion medicine,the cooperation through pediatric hematologists and surgeons in the perioperative period,more and more operation of children accompany coagulation factor deficiency can be safety finished.Different types of coagulation factor deficiency in children are treated by high-purity clotting factor,in order to correct abnormal coagulation factors levels.Clinical data showed that coagulation factor deficiency in children after proper treatment can be safely performed surgical procedure.