A database model of ontology was established by mining the EMR-covered data according to the con-cept of fuzzy vector space model with Kawasaki disease as an example. The evidence and model for the diagnosis and treatment of Kawasaki disease were detected fromthe EMR of Kawasaki disease in the database model of ontolo-gy in order to help the clinicians to make its differential diagnosis and improve its treatment plan, thus achieving the effective EMR data mining and promoting the computer-aided clinical decision-making.

Objective To explore the effect of hepatocyte growth factor/scatter factor (HGF/SF) on apoptosis of colorectal cancer cells induced with curcumin. Methods MTT assay was used to evaluate the cytotoxicity of curcumin to colorectal cancer cells. Flow cytometry was used to detect the anti-apoptosis effect of HGF. Results Flow cytometry showed only 64 ?g/ml curcumin could play the proliferation-inhibiting role in Caco-2 cells leading to their apoptosis; at the same time, different concentrations of HGF could antagonize this inhibitory effect resulting in the decrease of apoptosis, but HGF worked without a concentration-dependent manner. The study on MAPK pathway showed that the protective effect of HGF on the apoptosis of Caco-2 cells was not influenced by inhibiting p42/p44 MAPK and p38 MAPK pathway. Conclusion HGF/SF antagonizes the apoptosis of Caco-2 cells induced with curcumin, but MAPK signaling pathway might not participate in this process.

As the economic reformation goes on, the traditional culture and ethics anr chanllenged. Should we carry on the spirit of Betheune? The hospital, cultural development focuses on how to carry forward the spirit of Betheune. Acording to his experience, the author explained his view and put several points that we should notice in new situation. 1. Emancipate the mind and change the view; 2. Advocate the unselfish contribution combined with the policy of contribution considering oneself; 3. Requine the level of conbined with regulatiton.

Objective To document the feasibility and safety of percutaneous lung cancercryotherapy(PLC)guided by Computer Tomography(CT).Methods 798 CT-guided PLCs in 634 cases with lung cancer from August,2001,to April,2006.CT-visualized immediate ice formation beyond tumor margins was compared to tumor size and location.PLC feasibility and safety was evaluated for the complications postcryoablation.Results All the patients underwent PLC using local anesthesia with minimal or no sedation.Iceball formation was well seen as negative Hounsfield units within soft tissue masses.Tumor size and location were independent determinants of ice coverage:96.4%(n= 343)for masses 4 cm in diameter(P

To explore the effects of psychological behavior training on the physical stress level of submariners, 42 submariners were divided into two groups, namely experimental group and control group. The submariners in the former group received psychological behavior training for one week. The level of physical stress was determined after being asked to creep through water-filled torpedo launching tube before and after training. The results showed that the changes in physical stress level of subjects in the experimental group were significantly smaller than that of the control group(t=-3.421～-2.415, P

In order to investigate the dynamic changes in nitric oxide synthase (NOS) activity in organs during traumatic shock and to evaluate the effects of inhibitor of NOS and L Arginine (L Arg) on traumatic shock, rat model of traumatic shock was established, and the constitutive NOS and inducible NOS in the heart, small intestine, liver, lung , spleen were measured 0 5h, 3h, 5h after traumatic shock. Followed by intravenous injection with aminoguanidine (AG ), N G nitro L arginine methyl ester (L NAME), or L Arg, NOS were again measured, and the survival time and 24h survival rate were recorded. It was found that in normal rats cNOS was expressed in all organs, but only a weak iNOS expression in lung and liver. Half an hour after shock, cNOS was elevated in almost all organs in various degrees but there was no change in iNOS. 3h after shock, an increase in iNOS activity and a decrease in cNOS were observed. The iNOS was synthesized in large quantity 5h after shock. AG and L Arg markedly prolonged the survival time but L NAME did not in shock rats. AG inhibited the activity of iNOS but promoted the synthesis of cNOS, and both NOS were inhibited in L NAME group, but not in L Arg group. The results that iNOS was synthesized in large quantity only at later period of traumatic shock. To treat shock, the inhibitors of NOS and L Arg should be given according to the degree of shock, the dosage of drugs, and the time of administration

Objective To study the function of hepatocyte growth factor/scatter factor(HGF/SF) in the (proliferation) of colorectal cancer cells.Methods The expression of c-met,the receptor of HGF,was(detected) in Caco-2 and Colo320 cell lines by Western blot.The activation of p42/p44MAPK and p38MAPK induced by HGF in these two cell lines was observed.Observation of the effect of the inhibitor of p42/p44MAPK(PD98059),p38MAPK(SB203580) on the inhibition of HGF-induced proliferation of Caco-2 and Colo320 cells were made by Using -TdR,MTT assay.Results(1)Both cell lines(expressed) the c-met.(2)HGF activated p42/p44MAPK and p38MAPK,and 20ng/ml HGF treated cells showed maximum activity in both to be within 10min.(p42/p44MAPK,2.28?0.01;p38MAPK,2.25?0.01).(3)HGF was found to significantly increase thymidine incorporation(P

Objective To investigate the inhibitory effects of traditional Chinese medicine (TCM) on hypermelanosis and provide experimental evidence for treating skin pigmentary disorders. Methods Five TCMs with strong inhibitory effects on tyrosinase activity were tested. The changes of the number and morphology of melanocytes induced by UVB were observed in the experimental hypermelanosis model (brownish guinea pig). Results Decreased melanocytes and melanin granules were found with the treatment of Poria cocos, Polyporus umbellatus, Cornus officinalis Sieb. et Zucc., Atractylodes macrocephala Koidz., Astoagalus complanatus R. Br. ex Bge. Conclusion There are inhibitory effects on hypermelanosis induced by UVB with the treatment of Poria cocs, Polyporus umbellatus, Cornus officinalis Sieb.et Zucc., Atractylodes macrocephala Koidz., Astoagalus complanatus R. Br.ex Bge.

Objective To explore the safety and efficacy of loop ileostomy and Hartmann operation in gerontal patients with obstructive carcinoma of sigmoid and high rectum. Methods sixty-two gerontal patients with obstructive carcinoma of sigmoid and high rectum from April 2008 to April 2013 were randomly divided into loop ileostomy group and Hartmann group.The operation time , length of stay and postoperative complications in the two groups were analyzed. Results The time of absolute diet in loop ileostomy group was shorter than that in Hartmann group in Stage I operation (P < 0.05). The time of operation, absolute diet, and length of stay in loop ileostomy group were shorter than that in Hartmann group in Stage II operation (P < 0.05). Conclusion The application of loop iloostomy in gerontal patients with obstructive carcinoma of sigmoid and high rectum was safe and effective.

Double template molecularly imprinted polymer ( D_MIP) was prepared by emulsion polymerization method using the nonylphenol (NP), bisphenol A (BPA) as templates, α_methyl methacrylate(MAA) as functional monomer. Then using the D_MIP as solid phase extraction materials, a method based on double_molecularly imprinted solid phase extraction coupled with high performance liquid chromatographic fluorescence detection was developed for the determination of trace NP and BPA from environmental and food samples. The D_MIP was characterized by Fourier transform infrared spectroscopy ( FT_IR ) and adsorption experiments. The results showed that the saturated adsorption capacity of the D_MIP for NP and BPA was 73. 3 and 97. 5 mg/g, respectively, and the relative selectivity coefficient was 2. 2 and 1. 7, respectively. Moreover, the conditions affecting extraction efficiency, such as washing conditions and desorption conditions were optimized. Under the optimal conditions, there was a good linear correlation in the concentration range of 0. 01-2. 3 mg/L with correlation coefficients (R2) greater than 0. 998 for NP and BPA. The detection limits ( S/N=3) were 0. 001 and 0. 002 mg/L. The proposed method was applied to determine NP and BPA in river water, beer and crucian carp samples. The recoveries of NP and BPA were in the range of 86. 4%-99. 1%with the relative standard deviation less than 6 . 2%. This method has good selectivity and high sensitivity and shows good prospects for the application in the enrichment and separation of NP and BPA in real samples.