TP5 and CsA were injected-intraperitoneally into groups of experimental mice, and NS was used for the contral group. Herpes simplex keratitis models were made subsequently by inoculation of the virus on the cornea. On the 4～6th day after inoculation, slit lamp examination showed that the corneal epithelial and stromal lesions in the TP5 group were significantly more severe than those in the NS group, whereas the group of CsA manifested no such differences. Among the 3 groups of mice, no differences were evident in the formation of corneal neovascularization, which all peaked on the 8th day. The conjuctival and palpebral lesions in the TP5 and CsA groups were also more severe than in NS group. Therefore, immunomodulators should be clinically used with care in accordance with the morbid varieties and conditions.

Pseudomonas aeruginosa was inoculated into corneal stroma of rabbits and the model of keratoconjunc tivitis was established. Empty liposomes were used in a control, group ofloxacin liposome and ofloxacin eyedrop groups as experimental ones. After treatment for six times conjunctival and corneal lesions were examinated. Swabs of conjunctival sac were cultured. Result: in conjunctiva, lesions in the two experimental groups were all significantly less than those in the control group on the 6th, 8th and 11th day. Lesions in the ofloxacin liposomes group were also significantly less than those in the ofloxacin eyedrop group on the6th day. In cornea, lesions in the ofloxacin liposomes group were significantly less than those in the control group on the 6 th, 8th and 11th day. Lesions in the ofloxacin eyedrop group were significantly less than those the control group on the 11th day. In the result of bacterium culture, the culture's positive rate of two experimental gronqs and control group had significant differences on the 6th day. On the 11th day, the positive rate the ofloxacin liposome groups and ofloxacin eyedrop groups increased, and in the ofloxaein liposomes group had a less increase. Conclusion: ofloxacin liposomes and ofloxacin eyedrops were effective in treatments of pseudomonas keratoconjuntivitis in the rabbit model, the effects of ofloxacin liposomes were better than that of ofloxacin eyedrops.

Objective To summarize nursing experience of pathological fracture of femoral shaft metastasis treated with external fixation and percutaneous bone cement injection, improve special nursing quality, and alleviate pain of patient effectively and improve whose life quality.Methods Performing psychological nursing for patients before operation, observing patients situation carefully after operation, nursing wound limbs, preventing complication and instructing functional exercise. Results The pain alleviated obviously in 13 patients after operation, no patient had complication in hospital period. 11 of 13 patients were followed up, which time were 2～14 months, and the pain disappeared in 9 cases, alleviated obviously in 2 patients. The patients could sit in wheelchair or walk with crutch. The patient life quality improved obviously. Conclusion Pathological fracture of femoral shaft metastasis treated with external fixation and percutaneous bone cement injection has the advantages of little expense and better analgesic effect. Early functional exercise can be instructed. The quality of life of the patients improved obviously through systemic individuation rehabilitation nursing.

Objective:To study the influence of regional population and health input on public hospital operation by empirical analysis.Methods:Taking Chifeng Municipal Hospital and Xilingol League Hospital as samples and applies contrastive analysis to this essay.Results:Chifeng Municipal Hospital had 4 times of patients and 2 times of total health input than Xilingol League Hospital.Chifeng Municipal Hospital could better receive the fund for general hospital operation and had little reliability on the fiscal.Conclusion:The public hospitals located in low population density area could not adopt the same medical reform policies as other hospitals in different areas.It needed to give support for medical insurance payment and price adjustment,especially for fiscal compensation.

Colorectal cancer is one of the most common malignancies in human.Early diagnosis is the key points in improving the prognosis of patients with colorectal cancer.The implementation of standardized operation and comprehensive treatment for advanced and late stage cancer can improve the outcome.Based on the pattern of lymph node metastasis and the concept of complete mesocolic excision,the operation of colonic cancer has entered the era of normalization and standardization.The surgical procedure of rectal cancer (especially low rectal cancer) should be individualized based on the gold standard of total mesorectal excision (TME).For hepatic metastasis of colorectal cancer,hepatectomy is currently the standard and the only potentially curative treatment method.Therefore,how to evaluate the resectability of hepatic metastasis of colorectal cancer is particularly important.Surgeons are the most important participants in the diagnosis and treatment of colorectal cancer,and standardized diagnosis and treatment is the key to improve the prognosis of patients.

The principle of R0 resection for colorectal cancer(CRC)has been widely accepted based on the preoperative imaging evaluation.The surgeons should scientifically and strictly assess resectability of tumors and furthest reduce tumor residuals.Patients with initial unresectable CRC will give priority to conversion therapy and then undergo R0 resection if converting into resectable tumors.Palliative operation for the patients who cannot receive R0 resection according to the clinical assessment should strictly follow surgical indications and achieve wellplanned and clear surgical purposes.There is no evidence that the palliative operation or cytoreductive surgery benefits to survival of patients,and decreasing complications and improving quality of life through minimum wound are the basic principles.

Objective: To study the effects of basic fibroblast growt h factor(rhbFGF), transforming growth factor-?1(rhTGF-?1) and bone morphogenet ic protein-2(rhBMP-2) on the alkaline phosphate and calcification capacity of rat bone marrow stromal cells(BMSCs) in vitro. Methods: Rat BMSCs were cultured in DMEM with the addition of rhbFGF(1 ?g/L), rhTGF-?1(5 ?g/L) and rhBMP-2(200 ?g/L) alone or in combination.Alkaline phosphoric activ ity of the cells was mesured after 12- and 14-day culture respectively. For th e test of calcification, BMSCs were cultured in minerialization inducing medium with the addtion of above mentioned cytokines, calcification capacity of the cel ls was examined with Vonkossa staining after 10,20 and 30 days culture respectiv ely.Results: rhBMP-2 strengthened the ALP activity, rhbFGF, rh TGF-? 1 alone or in combination inhibited ALP activity, rhTGF-? 1 combined w ith rhBMP-2 and rhbFGF combined with rhBMP-2 inhibited ALP activity;the 3 kind s of growth factor in combination inhibited ALP activity. Vonkossa staining reve aled that rhBMP-2 alone or combined with rhbFGF increased the calcification of BMCs(P

Objective:To evaluate the periodontal tissue regeneration effect of implantation of bone marrow stromal cells(BMSCs)-root-coralline hydroxyapatite(CHA) complex.Methods:BMSCs of beagle dogs were in vitro cultured on CHA scaffold and on tooth root surface respectively.Then BMSCs-root samples were inserted into BMSCs-CHA scaffolds to form BMSCs-CHA-root complexes.5 complexes were implanted on one side of mandible in each of the dogs 1 month after the 4th,5th and 6th teeth had been extracted.Root-CHA without BMSCs was implanted on another side as the control.All dogs were killed 16 weeks after implantation,and the periodontal tissue regeneration were observed histologically.Results:More new bone formation and more mature alveolar bone were observed in the test group than that in the control,and there was a clearly periodontal ligament-like tissue formation only in the test group.Conclusion:The complex of BMSC-root-CHA can improve the periodontal tissue regeneration.

砄bjective: To evaluate the diagnostic significance of exfoliative cytological examination and micronucleus assay. Methods: 26 cases of leukoplasia (LK) and 18 cases of oral squamous cell carcinoma (SCC) were examined. Cells were scrapped from the lesions, stained with Papaniceolaou. The keratinization types and the configuration of the cells were studied. Smears were also stained using the Feulgen plus fast green method. The frequency of micronucleated cells was counted. Results: Mixed keratinization was observed in all 15 cases of LK without dysplasia and in 7 out of 11 cases of LK with dysplasia. Parakeratosis was found in 17 out of 18 cases of SCC. There was a significant difference of cell keratinization types and cytologic diagnosis between the cases of LK and SCC( P 0.05).Conclusion: Parakeratosis in oral exfoliative cells may indicate a high risk of malignance.

Objective: To study the relationship of the expressions of new apoptosis-related gene (PDCD)5 and p53 in oral normal mucosa, oral leukoplakia and oral squamous cell carcinoma. Methods: The expressions of PDCD5 and p53 were observed separately in 17 samples of oral normal mucosa, 60 of oral leukoplakia, and 30 of oral squamous cell carcinoma by Immunohistochemical means. Results: PDCD5 positive rate in oral normal mucosa was 88.2%, in oral leukoplakia was 63.3%, and in oral squamous cell carcinoma was 30%. P53 positive rate in oral normal mucosa was 0, in oral leukoplakia was (31.7%), and in oral squamous cell carcinoma was 60%. There was a negative relationship between (PDCD5) SII and P53 SII in every lesion. Conclusion: It suggests that both PDCD5 and p53 could be used as molecular markers of carcinogenesis for oral epithelium.