Objective To analyze the results of syphilis test and to understand the syphilis infection and its epidemiological characteristics .Methods 728 untreated patients with definite diagnosis of syphilis were enrolled and classified according to their age ,gender and syphilis types ,and retrospectively analysis was conducted .Results Among 728 syphilis patients ,446(61 .26% ) were diagnosed with latent syphilis ,211 (28 .98% ) with dominant syphilis ,32(4 .40% ) with neurosyphilis and 39(5 .36% ) with congenital syphilis .The average detection rate of syphilis was 1 .49% .332(45 .60% ) cases were found in female patients while 396 (54 .40% ) in male .Male syphilis patients were mainly in the 20 to 60 age group ,and female in the 20 to 50 age group .Dominant syphilis occurred mainly in the 20 to 40 age group and latent syphilis in the 20 to 60 age group .Conclusion The detection rate of syphilis increases year by year ,with the highest growing rate in latent syphilis and young adults as major incidence groups .

Objective To explore the clinical value of laparoscopic hepatectomy in liver tumor surgery.Methods The clinical data and follow-up results of 50 patients with liver tumors treated by laparoscopic hepatectomy were retrospectively analyzed.Results Laparoscopic liver resection was successful in 30 cases of primary liver cancer,10 cases of hepatic hemangioma,and 10 cases of other benign liver space occupying lesions (liver vascular smooth fatty tumor,focal nodular hyperplasia,hepatic abscess).Conclusion Laparoscopic hepatectomy as a minimally invasive treatment method is applicable to all parts of liver tumor surgery,and trauma is small,recovery is quick,and it is safe and feasible,and clinical effect is reliable.

Objective To explore the feasibility and clinical effect of single-incision laparoscopic surgery(SILS) in the treatment of gastrointestinal stromal tumors (GIST).Methods Clinical data of 13 GIST patients who underwent SILS resection [tumor diameter (1.3 ± 0.5) cm] were retrospectively analyzed.Surgical method was summarized and the clinical outcome was evaluated.Results All patients were successfully implemented without open conversion.The incision length was (3.8 ± 0.6) cm.Operation time was (100.0 ± 34.6) min.Intraoperative blood was (70.0 ± 45.5) ml,hospitalization time was 1 ～ 4d.There were no intraoperative or postoperative complications,such as secondary haemorrhage,anastomotic leakage or obstruction.The patients were favorably healed.Conclusion Application of SILS in treatment of GIST is safe with small trauma,fast postoperative rehabilitation,and early curative effect is satisfactory.

Objective To explore the causes and prevention methods of postoperative anastomotic leakage after colorectal cancer laparoscopic-assisted surgery.Methods 100 patients with colorectal cancer underwent laparoscopic-assisted surgery,the data was collected and retrospectively analyzed.Results All patients were successfully done,there were 5 cases with anastomotic leakage (5％).There were significant correlations of anastomotic leakage with too strong anastomotic tension,insufficiency anastomotic blood supply,tumor location,size,operation time,preoperative radiotherapy,gender,age,obesity,diabetes,hypoalbuminemia,and so on.Conclusion The laparoscopic-assisted surgery of colorectal cancer has a curative efficacy,with the continuous improvement of laparoscopic technique and operation,as long as the proper measures were taken,the occurrence of anastomotic leakage can be prevented and reduced.

As the key phase for medical students to be doctors,clinical internship plays a vital role in the whole medical education process.Interns in the department of gastrointestinal surgery face series of difficulties,such as wide variety of diseases and emergence of new technology and new concepts.In addition,because of the uneven levels of teachers and low enthusiasm of interns,the result of clinical practice teaching is not satisfactory.Based on the above-mentioned problems,measures have been introduced during the process of clinical practice teaching,including selection of typical diseases and surgical procedures,construction of clinical practice teaching team,adoption of pluralistic teaching methods,reinforcement of participation of interns during the clinical practice.Finally,the quality of teaching in our department has been irmproved significandy.

ObjectiveElectric stimulation of the central nervous system has been served as a treatment method for variety of neurological,psychiatric and sensory disorders.Despite considerable success in some applications,current stimulation techniques offer little control over which neuronal targets are activated by stimulation.This study aimed to present a new shape self-adaptive helix electrode for selective activation of optic nerve.MethodsThe geometric model of optic nerve and new helix electrode was elaborated with COMSOL Multiphysics.The new helix electrodes consist of silicone helix frame,which acted as support and insulation,and platinum contacts embedded within the frame.The activating function (AF) was introduced to characterize the stimulation effects,and the selectivity of activating optic nerve fascicle with new helix electrode was simulated by COMSOL Multiphysics,taking into account the variations of electrode contact locations.ResultsAssuming normalized AF threshold was 0.1 V/m2,the ratio difference of AF over threshold between new helix electrode and traditional electrode was only 1.2410％.With contacts in two ends of helix electrode closer to the middle contact,the small nerve fascicle was first activated and then the large one.ConclusionThe results show that the new helix electrodes have the same stimulation effects as that of traditional cuff electrodes.The new helix electrodes can selectively activate optic nerve fascicle with variations of electrode contact locations.

Objective To evlauate the clinical feasibility and efficacy of laparoscopic right hemicolectomy in the treatment of colon carcinoma .Methods 100 patients with rightcolon carcinoma were divided into 2 groups,50 ca-ses in each group .The control group received open surgery ,and the observation group was treated by laparoscopic right hemicolectomy .The clinical effect and side effect of the two groups were compared .Results The patients of the two groups were all successfully operated .The intraoperative blood loss ,postoperative anal exhaust time ,postoperative hos-pital stay,incidence rate of postoperative complications in the observation group were (75 ±42)mL,(24.8 ±1.3)h, (7.5 ±2.0)d,4.0%,respectively,which were significantly better than those of the control group [(100 ±50)mL, (64.9 ±1.8)h,(12.1 ±3.4)d,10.0%](χ2 =5.02,9.28,11.70,9.87,all P<0.05);Conclusion The laparo-scopic right hemicolectomy in the treatment of rightcolon carcinoma is safe and feasible ,and the curative efficacy is outstanding .It can obviously improve the prognosis of patients ,which should be popularized and applied in clinical .

Objective To investigate the effects of rhubarb combined with glutamine on repair of gut mucosal barrier injury after gastrointestinal surgery in rats. Methods Fifty male healthy Sprague-Dawley(SD)rats were randomly divided into five groups:control(C group),enteral nutrition(EN group),rhubarb with EN(R-EN group), glutamine with EN(G-EN group)and rhubarb combined with glutamine and EN(R-G-EN group)groups(each n=10). The rats of C group received no treatment and were fed normally. Cecectomy and gastrostomy were performed in EN,R-EN,G-EN and R-G-EN groups,and in 24 hours after surgery,they were interfered with the use of amoxicillin 50 mg/d and metronidazole 20 mg twice a day. From the first day after operation,each group was treated with the same prescription of enteral isocaloric and isonitrogenous nutrition for consecutive 7 days. Seven days later, under anaesthesia,laparotomy was performed,and a specimen of 1 cm intestinal segment was taken from the terminal 5 cm long ileum for all the groups;under the light microscope,the morphological examination of intestinal mucosal epithelial tight junctions(including villus height and mucosal thickness)was carried out. For each group,enzyme-linked immunosorbent assay(ELISA)was used to measure the levels of plasma citruline and D-lactate,and the serum concentration of TNF-αwas detected by utilizing double antibody ABC-ELISA. Results The levels of D-lactate and TNF-αin EN,R-EN,G-EN and R-G-EN groups were obviously higher than those in C group(all P<0.01). The level of plasma citruline(μmol/L:16.13±0.58 vs. 14.52±0.28)of R-G-EN group was remarkably higher than that in R-EN group(P<0.05),and its levels of serum D-lactate(mmol/L:3.12±0.56 vs. 3.62±0.52)and TNF-α(ng/L:2.82±0.62 vs. 3.54±0.49)were markedly lower than those of G-EN group(both P<0.05). The mucosal villus height(μm:126.50±6.31 vs. 175.67±11.91),mucosal thickness(μm:223.67±11.94 vs. 277.00±14.06)and the level of plasma citruline(μmol/L:13.25±0.36 vs. 15.67±0.55)in EN group were obviously lower than those of C group(all P<0.01);the above indexes of R-EN,G-EN and R-G-EN groups were higher than those of EN group (P<0.05 or P<0.01),but in the comparisons of the villus height and mucosal thickness among the three groups there were no statistical significant differences(all P>0.05). Conclusion The application of rhubarb combined with glutamine can protect gut mucosa,reduce the level of inflammatory reaction and elevate the level of plasma citruline, that is beneficial to the repair of gut mucosal barrier injury and its functional recovery after gastrointestinal surgery in rats.

Objective To study the relationships among postpartum depression,dysfunctional attitudes and automatic thoughts.Methods 274 cases of postpartum women were estimated at fifth or sixth week after delivery by Edinburgh Postnatal Depression Scale (EPDS),Dysfunctional Attitudes Scales (DAS),Automatic Thoughts Questionnaire(ATQ) and some other forms designed by ourselves.Results ①The prevalence of postpartum depression was 18.20％.②The impact of culture degree on postpartum depression was statistically significant(x2 =12.37,P ＜ 0.05).③Compared with normal subjects,the postpartum depression group had significantly higher total score of ATQ (48.98 ± 12.35 vs 79.29 ± 23.90),total scores of DAS (118.92 ± 20.03 vs 148.03 ± 28.47),attraction or repulsion (13.82 ± 4.33 vs 18.01 ± 6.38),perfection (14.53 ± 5.91 vs 19.21 ± 5.23),compulsion(13.09 ± 3.61 vs 19.36 ± 4.98),seeking applause (14.97 ± 4.06 vs 18.78 ± 5.59),dependence (14.30 ±4.12 vs 19.23 ± 5.43),autonomous attitude(16.54 ± 3.67 vs 21.51 ± 4.81),cognition philosophy (12.32 ± 3.79 vs 18.16 ± 3.43),the difference was all statistically significant (P ＜ 0.01).④There was a significant positive correlation in postpartum depression and total scores of ATQ,total scores of DAS,attraction or repulsion,perfection,compulsion,seeking applause,dependence,cognition philosophy (the Pearson correlation was respectively:0.658,0.432,0.187,0.432,0.481,0.304,0.458,0.459,P ＜ 0.01).⑤Through stepwise regression analysis,automatic thoughts,cognition philosophy,compulsion,perfection and dysfunctional attitudes might play considerable role to predict the occurrence of postpartum depression.Conclusion Automatic thoughts,dysfunctional attitudes can predict postpartum depression to some extent.

Objective To explore the microanatomy technique of the endoscopy-assisted extreme lateral supracerebellar infratentorial keyhole approach and clinical significance.Methods The extreme lateral supracerebellar infratentorial keyhole approach was imitated in 10 vascular perfusion adult cadaveric heads fixed in formalin,the dorsolateral mesencephalon region was dissected and observed by microscopy and neuroendoscopy from December 2012 to March 2013.Results The extreme lateral supracerebellar infratentorial keyhole approach could be fully exposed the ambient cisterns,the posteromedial tentorial incisura,the dorsolateral mesencephalon region,the trochlear nerve,the root of the trigeminal nerve,the superior cerebellar artery,the posterior cerebral artery and other important anatomical structures.The temporal lobe and parahippocampal gyrus could be exposed when the tentorial was incised.The application of neuroendoscopy could clearly reveal the trigeminal nerve and surrounding structures.Conclusion The extreme lateral supracerebellar infratentorial keyhole approach is suitable for the surgical resection in dorsolateral mesencephalon region.The application of neuroendoscopy can make up for the lack of the trigeminal nerve observed under microscopy and expand the operative field.