The development of Chinese herbs in a modernized context has been an endeavor of traditional Chinese medicine (TCM) industry for many years. Studies on active fractions and functional mechanism of Chinese herbs based on reductionism have been dominated for many years in modernization research of Chinese herbs, due to its multi-component and multi-targets nature. Fortunately, systemic theory of TCM is in a position to provide novel insights for Chinese herbs study. Based on systemic theory of TCM, this paper discussed the development of Chinese herbs combining with research ideas of“Chinese medicalization of western medicine” and“Chinese medicalization of chemicals”.

Objective To study the clinical application effect of Ivor-Lewis combined with thoracic-lapa-roscopy for esophageal carcinoma resection in patients with esophageal lowest cancer.Methods A total of 68 patients with esophageal lowest cancer were individed into the observation group and control group by the random number method,each of 34 cases.The patients in the observation group received Ivor-Lewis combined with thoracic-lapa-roscopy,and the patients in the control group received intrathoracic esophagogastrostomy by left chest posterolateral incision,then to compare the differences of clinical effect and complications.Results The operation time,blood loss in-operation,in-hospital time,thoracic drainage after operation in the observation group were all significantly less than those of the control group[operation time:(165.3 ±53.7)vs (214.3 ±71.5)min,t=3.814,P=0.037;blood loss in-operation:(153.6 ±23.1)vs (312.3 ±48.6)min,t=3.413,P=0.042;in-hospital time:(7.4 ±1.2)vs (10.5 ±2.3)d,t=3.256,P=0.043;thoracic drainage after operation:(635.6 ±34.2)vs (831.3 ±45.6)mL,t=2.594,P=0.039].The total rate of complications including pulmonary infection,gastric emptying,anastomotic fistula and diaphragmatic hernia and the recurrence rate after 6 months including recurrence in situ,lymph node and hema-togenous metastasis were all significantly lower in the observation group(complication:17.65%vs 38.24%,χ2 =4.012, P<0.001;recurrence:20.59% vs 47.06%,χ2 =4.825,P<0.001).Conclusion Ivor-Lewis combined with tho-racic-laparoscopy for esophageal carcinoma resection in patients with esophageal lowest cancer is safe and effective.

BACKGROUND:64-slice CT reconstruction technique for tibial plateau fractures hasobtainedsatisfactory results. This technology can reconstruct three-dimensional fracture relations, provide more scientific positioning and navigation standards for physicians, and improve the clinical cure rate. However, in the clinic, navigation method of 64-slice CT reconstruction techniques for tibial plateau fractures lacks a unified standard. OBJECTIVE:To study the navigation method and effect of 64-slice CT reconstruction in tibial plateau fractures. METHODS:A total of 44 patients with tibial plateau fractures were enroled in this study, including 27 males and 17 females, at the age between 21 and 74 years old. These patients were randomly divided into two groups (n=22). The control group underwent navigation method according to physician’s experience. The test group underwent navigation method according to 64-slice spiral CT scan results. Healing time, ful weight-bearing time, knee motion rangeat1 year postoperatively, the recovery ofknee functionat1 year postoperatively and adverse reaction were compared between the two groups. RESULTS AND CONCLUSION:(1) No significant differences in ful weight-bearing time and knee motion rangeat1 year postoperatively were detected between the two groups (P> 0.05). Healing time was significantly shorter in the test group than in the control group (P< 0.05). (2) The excelent and good rate of knee joint was 100% at 1 year postoperatively in the test group, which was significantly higher thanthe control group (81%) (P< 0.05). (3) The complication rate was significantly lower in the test group (8%) than in the control group (18%) (P< 0.05). (4) These results indicate that the effect of 64-slice CT reconstruction for navigation in tibial plateau fractures was ideal, could clearly simulate the pedicle screw fixation channel, visibly display anatomic characteristics of tibial plateau fractures, improve internal fixation accuracy, and improve repair effect.

Two thousands six hundreds and forty-four patients of malignant tumors of the breast were diagnosed and treated in our hospital from 1956 to 1990. Twenty-one cases of them were diagnosed mammary sarcoma by pathology in 0. 8% in contcmporary malignant tumors of the breast. The axillary lymph nodes of 6 cases in this group are clinically enlarged in 28. 6%, all of them were proved benign through mieroscopie examination. The age of the patient of mammary sarcoma is younger than that of the patient of mammary careinoma in the group. The patient's age over forty-five years and more than one year course have a hetter prognosis. Mammary sarcoma metastasizes by blood stream or local infiltrate, rarely metastasizes to regional lymph nodes. Mammary sarcoma is treated by removal of the tumor and simple mastectomy. The value of radiotherapy will be futher explored by practice.

Objective To investigate the MRI features and pathological findings of primary intracranial lymphoma and to improve the diagnostic accuracy.Methods MRI features and pathological findings were analyzed in 28 pathologically proved cases of primary intracranial lymphoma.All 28 cases were examined by MRI scanning and diffusion weighted imaging(DWI),among which 19 cases were examined by enhanced MRI scanning.Results MRI manifestations:(1)single type:intracranial single lesion,the most common site was nearly the middle area of deep brain or brain surface;(2)multiple type.Type Ⅱa:multiple lesions in regional distribution, often showed several small lesions around a larger lesion.Type Ⅱb:multiple lesions in diffused distribution,often showed scattered distribution without an obvious regional distribution.The shapes of lesion were mass-like,nodular or irregular.The lesions showed isointense or slightly hypointense on T1 WI,and isointense,slightly hyperintense or slightly hypointense on T2 WI.On DWI,the le-sions showed hyperintense.Most lesions showed obvious enhancement with fisting sign,incision sign,pointed sign,butterfly sign,long and stiff spicule signand comb sign.Pathology:the tumor cells were heteromorphic and spread around the vessels, and showed greater cellularity,big prominent nucleoli,and small lymphocytes.The immunohistochemical examination showed that all tumor cells were positive for CD20 and CD79a and negative for GFAP.Conclusion The MRI findings of primary intracranial lym-phoma are various.Careful analysis can improve the diagnostic accuracy of the disease,definitive diagnosis requires histopathological and immunohistochemical examinations.

Objective To investigate the effect of siRNA-down-regulating astrocyte elevated gene-1(AEG-1)on the proliferation and apoptosis of cervical carcinoma cells.Methods The designed and synthesized AEG-1 siRNA was transfected to the cervical car-cinoma SiHa cells.The influence of AEG-1 siRNA on the expression of AEG-1 gene was detected by real-time fluorescent quantita-tive-polymerase chain reaction (RT-PCR);the inhibiting effect of AEG-1 siRNA on the proliferation of cervical cancer SiHa cells was detected by the MTT method;the influence of AEG-1 siRNA on apoptosis and cell cycle of the cervical cancer SiHa cellswas determined by the flow cytometry.Results Compared with the control group,AEG-1 siRNA transfecting human cervical cancer Si-Ha cells could evidently down-regulate the expression of AEG-1 gene with statistical difference (P<0.01);at the same time,the dwn-regulation of AEG-1 gene expression could significantly inhibit the proliferation of the cervical cancer SiHa cells (P<0.01), promote the apoptosis of SiHa cells (P<0.01)and arrest the cell cycle in G0/G1 phase.Conclusion AEG-1 siRNA can down-regulate the expression of AEG-1 gene in the cervical carcinoma SiHa cells,inhibit the proliferation of the cervical carcinoma SiHa cells and promote their apoptosis.

Objective To investigate the effects of intensive insulin therapy on prognosis of patients with severe traumatic brain injury.Methods 130 patients with severe traumatic brain injury(GCS score 3-8) were randomly divided into the observation group (65 cases) and the control group (65 cases).The observation group received intensive insulin therapy,while the control group received conventional insulin therapy.The infection rate,hospital stay,in-hospital mortality and the 6-month mortality were observed.Results The infection rate of the observation group (32.4％) was significantly lower than that of the control group (46.7％) (x2 =1.946,P ＜ 0.05).The hospital stay of the observation group was significantly shorter than that of the control group (t =9.247,P ＜ 0.05).The inhospital mortality and the 6-month mortality were similar in the two groups(x2 =0.086,0.484,all P ＞ 0.05).The neurologic outcome according to Glasgow Outcome Score(GOS 4-5) at 6 months in the observation group was better than that in the control group(x2 =6.407,P ＜ 0.05).Conclusion Intensive insulin therapy can improve the prognosis of patients with severe traumatic brain injury.

Objective To evaluate the role of EP_2 in synaptic transmission in dentate gyrus of hippocampus. Methods Field excitatory post-synaptic potentials (fEPSP) were recorded at the perforated path-granule neurons in dentate gyrus in vitro. Results ① Butaprost, an agonist of EP_2, enhanced the synaptic transmission in dentate gyrus and decreased the paired-pulse ratio, and these effects were reversed by bath application of AH6809 (EP_2 antagonist). ② Application of Forskolin alone or with AH6809 elevated the slope of fEPSP. ③ The Butaprost-induced responses were mediated via PKA, ERK and IP_3 signal pathways. Conclusion Multiple signal pathways were involved in the EP_2 activation-mediated enhancement of synaptic transmission.

Objective To explore the role of cyclooxgense-2 (COX-2) in tetrahydrocannabinol (THC)-induced inhibition of long-term depression (LTD) in CA1 area in vitro. Methods The hippocampal slices were prepared at 24 h after intraperitoneal injection of Δ~9-THC (10 mg/kg) or COX-2 inhibitor NS398 (10 mg/kg). Field excitatory post-synaptic potentials (fEPSP) were recorded in the stratum radiatum of the CA1 area in vitro to observe the effect of NS398, a selective inhibitor of COX-2, on the THC-induced inhibition of LTD and THC's effect on membrane excitability in pyramidal neurons by using the whole-cell patch clamp technique. Results ① Low-frequency stimulation (1 Hz, 15 min) induced-LTD in CA1 area was significantly attenuated by Δ9-THC. ② Δ~9-THC did not affect the basal synaptic transmission and membrane excitability (including membrane resting potentials, input resistance and firing property). ③ THC-induced inhibition of LTD was reversed by NS398. ④ THC-induced inhibition of LTD was robustly impaired in COX-2 knockout mice. Conclusion THC-induced inhibition of LTD in CA1 area was mediated via COX-2.

Objective To study the clinical efficacy of canalith reposition on benign paroxysmal positional vertigo(BPPV).Methods In the eighty patients with BPPV,the 45 cases with anterior semicircular canal were treated with Epley reposition method and the other 35 patients with the horizontal semicircular canal were treated with barbecue tumble method.The clinical efficacy was observed.Results The effective rate of the Epley reposition method for anterior semicircular canal patients was 95.5％ (43/45) and the effective rate of the barbecue tumble method for horizontal semicircular canal patients was 91.4％ (32/35).Conclusion The canalith reposition method treated with BPPV patients has high clinical efficacy,simple procedures,and no obvious adverse reactions.