Objective To evaluate the role of EP_2 in synaptic transmission in dentate gyrus of hippocampus. Methods Field excitatory post-synaptic potentials (fEPSP) were recorded at the perforated path-granule neurons in dentate gyrus in vitro. Results ① Butaprost, an agonist of EP_2, enhanced the synaptic transmission in dentate gyrus and decreased the paired-pulse ratio, and these effects were reversed by bath application of AH6809 (EP_2 antagonist). ② Application of Forskolin alone or with AH6809 elevated the slope of fEPSP. ③ The Butaprost-induced responses were mediated via PKA, ERK and IP_3 signal pathways. Conclusion Multiple signal pathways were involved in the EP_2 activation-mediated enhancement of synaptic transmission.

Objective To explore the role of cyclooxgense-2 (COX-2) in tetrahydrocannabinol (THC)-induced inhibition of long-term depression (LTD) in CA1 area in vitro. Methods The hippocampal slices were prepared at 24 h after intraperitoneal injection of Δ~9-THC (10 mg/kg) or COX-2 inhibitor NS398 (10 mg/kg). Field excitatory post-synaptic potentials (fEPSP) were recorded in the stratum radiatum of the CA1 area in vitro to observe the effect of NS398, a selective inhibitor of COX-2, on the THC-induced inhibition of LTD and THC's effect on membrane excitability in pyramidal neurons by using the whole-cell patch clamp technique. Results ① Low-frequency stimulation (1 Hz, 15 min) induced-LTD in CA1 area was significantly attenuated by Δ9-THC. ② Δ~9-THC did not affect the basal synaptic transmission and membrane excitability (including membrane resting potentials, input resistance and firing property). ③ THC-induced inhibition of LTD was reversed by NS398. ④ THC-induced inhibition of LTD was robustly impaired in COX-2 knockout mice. Conclusion THC-induced inhibition of LTD in CA1 area was mediated via COX-2.

To discuss the common failures of PHILIPS DR and KODAK DR7500, analyze the working principle and failure phenomenon of the device, and then propose the exclusion and problem-solving methods to prove the accuracy through practice. The paper also focused on the correct use of DR and how the equipment maintenance staff to do a good job of maintenance and preventive maintenance , reduce unexpected failures and prolong the equipment life, provide reference for counterparts involved in the DR maintenance

The changes in tight junction proteins expression have effects on the function of the intestinal barrier.It is an important factor involved in the pathogenesis of inflammatory bowel disease.The levels of expression of tight junction proteins have been detected by different techniques in inflammatory bowel disease.The mechanism which leads to changes of tight junction proteins is still unknown completely.It is helpful to understand the pathogenesis of inflammatory of bowel disease by discussing the mechanism of changes in tight junction proteins.Improving expression of tight junction proteins is one of our strategies to treat inflammatory bowel disease,and some drugs have been identified.

0.05).The results showed that patients treated with Damon3TM brackets pay significantly lower treat- ment time(4.23 months),required significantly lower chairside time(137.15s)than those treated with conventionally-ligated brackets.Conclusions:In matched cases,Damon3TM brackets produced significant reductions in treatment time and the chair- side time,but gained the same treatment results comparing with conventionally-ligated brackets.

The decision-making problems of buying medical equipment in hospitals are given. With Dempster fusion of evidence decision-making theory, according to the expert judgment complexion for buying large-scale medical equipment on technical performance, price, maintenance cost, matching degree, economical efficiency and qualify, basic probability partition function and computational belief function are established, and then achieves advantage connection on candidate equipment and gives support for buying medical equipment in hospital.

Pseudomonas aeruginosa was inoculated into corneal stroma of rabbits and the model of keratoconjunc tivitis was established. Empty liposomes were used in a control, group ofloxacin liposome and ofloxacin eyedrop groups as experimental ones. After treatment for six times conjunctival and corneal lesions were examinated. Swabs of conjunctival sac were cultured. Result: in conjunctiva, lesions in the two experimental groups were all significantly less than those in the control group on the 6th, 8th and 11th day. Lesions in the ofloxacin liposomes group were also significantly less than those in the ofloxacin eyedrop group on the6th day. In cornea, lesions in the ofloxacin liposomes group were significantly less than those in the control group on the 6 th, 8th and 11th day. Lesions in the ofloxacin eyedrop group were significantly less than those the control group on the 11th day. In the result of bacterium culture, the culture's positive rate of two experimental gronqs and control group had significant differences on the 6th day. On the 11th day, the positive rate the ofloxacin liposome groups and ofloxacin eyedrop groups increased, and in the ofloxaein liposomes group had a less increase. Conclusion: ofloxacin liposomes and ofloxacin eyedrops were effective in treatments of pseudomonas keratoconjuntivitis in the rabbit model, the effects of ofloxacin liposomes were better than that of ofloxacin eyedrops.

Objective To investigate the effect of siRNA-down-regulating astrocyte elevated gene-1(AEG-1)on the proliferation and apoptosis of cervical carcinoma cells.Methods The designed and synthesized AEG-1 siRNA was transfected to the cervical car-cinoma SiHa cells.The influence of AEG-1 siRNA on the expression of AEG-1 gene was detected by real-time fluorescent quantita-tive-polymerase chain reaction (RT-PCR);the inhibiting effect of AEG-1 siRNA on the proliferation of cervical cancer SiHa cells was detected by the MTT method;the influence of AEG-1 siRNA on apoptosis and cell cycle of the cervical cancer SiHa cellswas determined by the flow cytometry.Results Compared with the control group,AEG-1 siRNA transfecting human cervical cancer Si-Ha cells could evidently down-regulate the expression of AEG-1 gene with statistical difference (P<0.01);at the same time,the dwn-regulation of AEG-1 gene expression could significantly inhibit the proliferation of the cervical cancer SiHa cells (P<0.01), promote the apoptosis of SiHa cells (P<0.01)and arrest the cell cycle in G0/G1 phase.Conclusion AEG-1 siRNA can down-regulate the expression of AEG-1 gene in the cervical carcinoma SiHa cells,inhibit the proliferation of the cervical carcinoma SiHa cells and promote their apoptosis.

BACKGROUND: In the past, much attention was paid mainly to the body symptoms like focal neurological impairment in stroke, but the accompanied psychological symptoms were ignored easily.OBJECTIVE: To investigate the psychosocial factor in acute cerebrovascular disease and the mental state after the attack.DESIGN: Observational case-control study based on patients and healthy people.SETTING: A neurology department in a university hospital.PARTICIPANTS: Totally 55 patients with stroke hospitalized in Neurology Department of Hospital Affiliated to Medical College of Shaoxing Art and Science College and 35 healthy people taking physical examination from January 2001 to December 2003 in the hospital as controls.METHODS: Patients with acute cerebrovascular disease certified by CT/MR and people in control group took symptoms checklist-90(SCL-90) test within a month after attack.MAIN OUTCOME MEASURES: SCL-90 scores of the two groups.RESULTS: The condition of mental state in patients with acute cerebrovascular disease was worse than that in control group( P ＜ 0.01 ). There existed obvious physical and mental reaction. It was found in subsection analysis that the size and location of the focus had relationship with the degree of mental disorder after attack and it was easier to have mental disorder if the affection lied in left cerebral hemisphere.CONCLUSION: It is necessary to give psychological intervention in prevention and cure of acute cerebrovascular disease. Recognizing bad effect of stroke on psychoactivity may help to guide the treatment.

ObjectiveTo study the maternal diet on neonatal weight and gestational diabetes,placental function and the impact of it. Methods93 cases of maternal gestational diabetes admitted to hospital with informed consent according to odd and even numbers randomly divided to observation group(50 case) and control group(43 cases) ,all accept conventional treatment,including exercise,health education and drug,observation group plus with diet control,compare the two sets of maternal placental maturity,amniotic fluid situation and the situation of fetal weight. ResultsObservation group with normal weight was 38 cases(76.0％ ) ,low body weight was 9 cases( 18.0％ ) ,huge children was 3 cases(6.0％ ) ,control group was 27 cases( 62.8％ ) ,2 cases(4.7％ ), 14 cases( 32.6％ ) ,two groups of great children birth rate had no significant difference( x2 =9.210,P =0. 002) ;placental maturity between the two groups showed no significant differenee( x2 =1. 034,3. 353 and 0. 433,P =0. 307,0. 067and 0. 510). Observation group normal amniotic fluid was 42 cases(84.0％ ) ,oligohydranmios was 6 cases( 12.0％ ) ,polyhydramnios was 2 cases(4.0％ ) ,the control group was 28 cases(65.1％ ) ,3 cases(7.0％ ) ,12 cases(27.9％ ) ,normal amniotic fluid and polyhydranmnios were significant differences between the two groups ( x2 =4. 429,8. 548, P =0. 035,0. 003 ).ConclusionDiet can reduce fetal macrosomia and the incidence of oligohydramnios, and it could ensure the maternal and child safety.