The Content of LT B_4 in the edge of 36 SD rats were determined by HPLC. It was found that the content of LTB_4 in the anternortem wounds is markedly increased, and has certain linear relationship with time of injury within one hour. No increasing of LTB_4 could be found in the postmortem wounds. It indicates that it is very valuable to estimate time of injury by determining LT B_4 content in wound edge, The content of LTB_4 in wound edge can be determined rapidly and accurately with HPLC.

Objective To evaluate the clinical efficacy and safety between Alprostadil regular therapy in the patients with ischemic stroke. Methods We searched the electronic databases (WANFANG DATA,CNKI,and OVID) in order to obtain the randomized controlled trial (RCT)with interventions of lipo-prostaglandin E1 from January 1,2003 to March 1,2011. The methodological quality of the trials was assessed using four standards:random allocation program,allocation concealment,blind and quit.A meta-analysis of included clinical trials was performed with Revman 5.0 software. Results 13 trials were included and the data were divided into 3 groups by different outcomes,the effective rate(12 trials),the neurological deficit score(CCS,6 trials) and the daily life score (4 trials). Meta-analysis showed that after treatment,there were differences in neurological deficit (OR:-4.76,95％ CI:8.46,-1.06)and daily life(OR:0.95,95％ CI:-1.52,-0.38) between lipo-PGE1 treatment group and regular treatment group.The effective rate were higher in lipo-PGE1 treatment group than in regular treatment group (OR:3.75,95％ CI:2.45,5.75).The improvement in the overall symptoms of ischemic stroke after lipo-PGE1 therapy was better than regular treatment.In the occurrence of adverse reactions,18 cases appeared in lipo-PGEl treatment group(among which 11 cases were vascular stimulating reactions after injection),1 case in regular treatment group. Conclusions Lipo-prostaglandin E1 is an effective drug for ischemic stroke in view of its improvements in cure rate,neurological deficit and activities of daily life.

The in vitro passage number and proliferation of non-immortalized cells are limited, which restrictions cell therapy or in vitro studies. Cells transfected with temperature sensitive simian virus 40 large T antigen (ts-SV40LT) gene could show the greatest proliferation. The cells can be amplified with compensating the lack of limited number of cells under the permissive temperature. Non-permissive temperature can be used in studying the cell therapy or its other physiological characteristics. This research field involves peritoneal stromal cells, satellite cells of urinary tract, oral epithelial cells, adre?nal medullary cells, bone marrow-derived endothelial cells, retinal progenitor cells, mesenchymal stem cells, hematopoietic stem cells, mast cells, podocytes and Kupffer cells. In this study, the current research on Ts-SV40-mediated temperature-sensitive cells was reviewed.

Objective:To assess coronary stenosis using 256-slice spiral CT (CTA).Methods:The data of 126 pa-tients diagnosed as coronary heart disease (CHD)using CTA and coronary angiography (CAG)Were retrospectively analyzed.The CAG results for CHD Were regarded as “gold standard”diagnosing CHD.The CTA diagnostic value for CHD and consistency of CAG and CTA diagnosing coronary stenosis Were analyzed.Results:A total of 393 cor-onary segments Were assessed using CTA.According to CAG results for CHD ,the CTA diagnostic accuracy Was 89.3%,sensitivity Was 91.4%,specificity Was 84.8%,negative predictive value Was 82.2% and positive predictive value Was 92.8%;the consistency of CAG and CTA diagnosing coronary stenosis Was good (Kappa value =0.755, P<0.01).Conclusion:The 256-slice spiral CT can diagnosing coronary stenosis,it possesses high sensitivity in di-agnosing vascular stenosis;as a simple and noninvasive examination it can be used as a reliable method for early screening subhealthy population and reexamine of putients With coronary heart disease.

Objective A rabbit model of common carotid artery grafted by external jugular veins was used. Vein grafts were transferred by c-myc antisense oligodeoxynucleotides(ODN) and carried by soluble stent. Try to find a new approach to prevent veins graft failure on molecular basis. Methods New Zealand rabbit were randomly divided into five groups, 10 animals each. Pretreating the soluble stents with synthesized c-myc ODN, following stents: (1) control group; (2) soluble stent; (3) soluble stent with sense-ODN; (4) soluble stent with antisense-ODN; (5) soluble stent with mismatch-ODN were put into the vein graft during end to end anastomosis. After 7, 28 and 90 days of operation, vein grafts were harvested. HE and ET stain were made aim to calculate the extent of intima hyperplasia. The expression of c-myc and PCNA were identified by immunchemistry methods. Situ hybridization and Northern Bloting were made to assess the expression of c-myc mRNA quantitatively and semi-quantitatively in veins. Results In the vein grafts of 7,28,90 days post-operation of soluble stent antisense-ODN group ①Intima hyperplasia were inhibited significantly compare with other four groups. ②The expression of c-myc and PCNA were inhibited significantly compare with other four groups. ③C-myc mRNA expression level was significantly Lower than the other four groups. Conclusion Soluble stent can transfer c-myc ODN effectively. C-myc antisense-ODN transferred by soluble stent can inhibit the expression of c-myc and PCNA, can inhibit intima hyperplasia of vein graft significantly, thus preventing the stenosis of vein graft.

Objective To evaluate the effects of allograft fusion cage(AFC) on anterior cervical interbody fusion. Methods AFCs were implanted in 61 degenerative cervical intervertebral spaces of 39 cases, who needed anterior cervical interbody fusion from September 1995 to December 1999. 31 cases were diagnosed as cervical spondylolysis， 2 cases as acute protrusion of cervical intervertebral disc and 6 cases as fracture and dislocation of cervical spine. The clinical effects and complications were observed, and the postoperative presentations of X－ ray examination of cervical spine were also evaluated. Results Thirty- nine cases were followed up with a mean period of 28.6 months. No neurologic complications appeared, and no AFCs shifted or dislocated. The clinical effects were satisfactory. 61 intervertebral spaces were confirmed to be solid fused completely by constant X－ ray examination at 3.9 months in average after operation. There were no collapse or angular deformities in 59 spaces of them,the other 2 spaces lost a little height because of removal of external fixation too early. Conclusion The implantation of AFC was simple, stable, less injury with similar intervertebral osseous fusion rate compared to the conventional anterior cervical interbody fusion. Forthermore, the implantation of AFC does not need auto iliac crest graft or the use of metal fixations. Some complications caused by implanting auto iliac crest and metal fixations can be avoided.

Objective To study the effect of brain-derived neurotrophic factor (BDNF) on the environmental nutrition and neural differentiation of the transplanted stem cells under hypothermia.Methods The BDNF gene mediated by liposome was transfected into 293T cell line, and ELISA assay was applied to find the peak time of BDNF expression. When BDNF was highly expressed, the supernatant was collected for establishment of SD rat models of brain injury. The rats were divided into Group A (stem cell transplantation group) and Group B (stem cell transplantation and BDNF group). Rats in both groups were under hypothermia treatment for five days. Four and eight days later ( three days from rewarming), rat brain tissues were obtained to detect the expressions of proliferating cell nuclear antigen (PCNA), nestin, neuron-specific enolase (NSE) and glial fibrillary acidic protein (GFAP) by immunohistochemical method and to detect the apoptosis by in situ hybridization. Finally, the nerve function scores were obtained for evaluation of the nerve function. Results The ELISA showed that the high level of BDNF expression was at 48 to 60 hours after gene transfection. PCNA and nestin were highly expressed, while NES and GFAP showed nil or low level of expression in both groups at the fourth day after hypothermia, with little apoptotic cells especially in the Group B (P ＜0.05). The expressions of PCNA and nestin were decreased, but the expressions of NSE and GFAP were increased at the third day after rewarming. The positive rate of NSE expression in the Group B was much higher and the apoptotic cells were much less compared with the Group A ( P ＜ 0. 05 ). A better nerve score was obtained in the Group B. Conclusion BDNF can enhance the survival rate of the transplanted stem cells and induce their differentiation into neurons under hypothermia.

Objective To explore the method of correctly placing and fixing mesh plug and patch in tension-free hernioplasty. Methods The data of 123 cases of plug-mesh tension-free hernioplasty with the method of placing and fixing mesh plug and patch were reviewed. Observed the incidence of postoperative effusion, infection, sinus formation, skin flap necrosis and hernia recurrence. Results All patients were restored after operation 8 days. Followed-up 1.5 -10.0 years,there was no incision effusion, infection,skin flap necrosis in all cases. No relapse happened on the operated side and 5 cases of inguinal hernia occurred on the non-operated side. Conclusions Mesh plug and patch must be placed to cover the whole abdominal wall defects,and contact closely with surrounding healthy tissue. They need to be placed neatly,fixed firmly and perpetually without any damage to the normal tissue structure.

Objective To study the clinical features and therapeutic method of severe cerebral injured patients with hyponatremia.Methods The electrolyte and central venous pressure were examined on 45 cases of severe cerebral injured patients with hyponatremia every day.According to plasma sodium value and central venous pressure,we regulated treatment perscription daily.Results 45 patients occured hyponatremia in total 288 of severe cerebral injured patients.Hyponatremia was detected 5～13 days after operation or after injure.The morbility is usually the highest in the seventh day.Plasma sodium recovered to normal value in 14 days after operation.Conclusion Severe cerebral injured patients with hyponatremia should be diagnosed and treated as early as possible,then it will receive better prognosis.

BACKGROUND:The self-expanding Smart nitinol stent system is a popular treatment for carotid artery stenosis, because it is easy to manipulate and deploy, and endothelialization is rapid. OBJECTIVE:To assess the efficacy of Smart nitinol stent system for the treatment of severe atherosclerotic carotid stenosis. METHODS:We conducted a retrospective, single-center, non-randomized, paral el control ed trial. A cohort of 103 patients with severe atherosclerotic carotid stenosis was included in the analysis after obtaining written informed consent from participants or their guardians. Treatment was undertaken according to each patient’s wishes after weighing the options:a Smart nitinol stent system (Cordis Corporation, Miami, FL, USA) was used in 40 patients, while 63 were managed conservatively with antiplatelet drugs. The primary outcome is the degree of disability of dependence 2 years after treatment, assessed by the modified Rankin Scale. The secondary outcomes are mRS scores 90 days and 1 year after treatment, recurrence of cerebrovascular events, and severity of neurologic deficit measured using the National Institutes of Health Stroke Scale 1 and 2 years after treatment. The study protocol was approved by the Ethics Committee of Beijing Jishuitan Hospital, China (approval number:201605-01) and conducted in accordance with the guidelines of the Declaration of Helsinki, formulated by the World Medical Association. This trial was registered at ClinicalTrial.gov (NCT02800174). DISCUSSION:Previous studies of the Smart nitinol stent system for the treatment of carotid stenosis are mostly self-control ed case series or smal cohort studies with short fol ow-up periods. Consequently, the long-term influence of Smart nitinol stent deployment on the risk of cerebrovascular events and long-term outcomes are not known. This trial il uminates the therapeutic benefits of the Smart nitinol stent system in a 2-year fol ow-up study involving a large cohort of patients with severe atherosclerotic carotid stenosis.