Mobile network is used to transmit signals for telemedicine dynamic ECG monitoring system.In this way,ECG monitoring can be performed to patients at all times and places,thus really realizing telemedicine dynamic ECG monitoring.In the paper,the system is studied.The discussion on how to transmit ECG signals and feedback the diagnostic message through mobile network is presented in detail.

BACKGROUND: The mechanism of central fatigue is unclear at present.The cumulation of lactic acid can play an important role in the central fatigue. Whether lactic acid can affect the neuronal functions, leading to the fatigue, needs to be examined.OBJECTIVE: To investigate the effects of lactic acid on the survival rate of primary neurons cultured from neonate rat cortex.DESIGN: Single sample observation SETTING: Laboratory of Cell Biology, Institute of Health and Environmental Medicine, Academy of Military Medical Science MATERIALS: Totally 40 Wistar neonate rats (within one day) provided by Experimental Animal Center of Academe of Military Medical Science were used in this study.METHODS: This experiment was conducted at the Laboratory of Cell Biology, Institute of Health and Environmental Medicine, Academy of Military Medical Science from October 2003 to May 2004. Primary neurons in added , and the pH value was decreased to 7.35,7.15,6.95 and 6.00,acid was added , and the pH value was kept at 7.35 , then the survival rate of neurons were observed. We analyzed the effect of lactic acid on neuron whether through down regulating pH value or the role of lactic acid.different concentrations of lactic acid and the same pH value on viability regulating pH value and lactic acid on neuron.pH. When PH values were 7.35,7.15,6.95 and 6.00, the survival rate of concentrations of lactic acid were 0,5,10 and 20 mmol/L,pH value was constantly kept at 7.35. Viabilities of neurons were 100%, 88%, 82%,81%, respectively, with significant difference as compared with control was significan tly lower than that of corresponding lactic acid group.CONCLUSION: A close relationship exists between pH decrease induced by lactic acid and viability of neurons. Excess lactic acid can have a toxic effect on neurons independently of t on the changes in levels of pH.

AIM: To explore the delayed protection of heme oxygenase-1 (HO-1) in the exercise preconditioning (EP) from the myocardial relative ischemia reperfusion injury (rI/R). METHODS: 40 Wistar Rats were divided into 5 groups randomly: control group (CN), rI/R group (IR), EP+rI/R group (EI), HO-1 inductor hemin+rI/R group (HE) and HO-1 inhibitor ZnPP+EP+rI/R group (EZ). The following indexes were detected, including the HO-1activity in myocardium, the cardiac function parameter-pressure-rate product (heart rate × left ventricular developed pressure, PRP) and the content of MDA in coronary effluent. RESULTS: After myocardial rI/R, HO-1 activity increased significantly. Moreover, EP or HO-1 inductor could enhance this effect manifestly. Nevertheless, when the HO-1 inhibitor was administered before EP,HO-1 activity decreased. In addition, there was no distinct difference in the HO-1 activity between EI group and HE group. At the 30 min point of reperfusion, the PRP recovery rate of EI group was higher clearly than that of IR group. However, there was reverse effect between the EZ group and the EI group. The MDA in coronary effluent of EI group, EZ group and HE group were lower obviously than that of IR group and there was significant difference between EI group and EZ group. CONCLUSION: EP could protect the heart from the rI/R injury occurring 24 hours later, which might be performed through activating the HO-1.

Objective In order to investigate the changes of glycogen and lactate in cortex after exercise-induced central system fatigue. Methods Central fatigue model was established according to Bedford weight/maximal oxygen uptake equation. The rats were divided into three groups: control group (C), sedentary-exhausted group (SE), and overtraining group (O). Results After 1 week exercise, compared with control group, the weight of rats in SE and O group increased slowly. BUN of SE and O group increased significantly to 4.78?1.99 mmol/L and 8.48 ?0.92 mmol/L respectively,but Hb showed no changes. Compared with C group, both DA and DA/5-HT of O group decreased dynamical which were 309.04?63.68 ng/ml?g and 0.42?0.25 respectively. The latency of SEP in O group distinctly delayed, P1: 2.98?0.55ms, N1: 4.23?0.35ms, P2: 5.28?0.60ms. Regarding to 5-HT, no significant difference was found among all groups. Cortex lactate concentration of O (1.46mg/100g )and SE(1.12 mg/100g) group were significantly higher than control group(0.92 mg/100g). There was a tendency of glycogen decrease but had no significant difference among all groups. Conclusion The results suggested that lactate and glycogen might have close relationship with central fatigue and were the possible reasons to cause central fatigue.

Objective:To investigate the duck thymocyte surface antigens.Methods:Repeated immunization of normal BALB/c mice with separated thymocyte from mature duck result in the production of mAbs which react with duck thymocyte surface antigens.Applying the immunohistochemistry assay,tracing the originating and distributing of lymphocytes which reacted with mAb in spleen,thymus,and bursa of duck different development stages.Results:Five monoclonal antibody-producing hybridomas(B6,B8,C12,E6,H5)were isolated.According to the result of immunohistochemistry assay,there were distributing strained lymphocytes in thymus,bursa and spleen of duck.The stained cells belonged to different populations.Conclusion:The mAb C12 reacted with the common cell surface marker of between mature T lymphocytes and dendritic cells.

Objective To determine the toxicity, maximal dose and clinical practicality of VM26 (teniposide) plus radiotherapy for postoperative brain neurospongioma. Methods Twenty patients were alloted in phase Ⅰ trial. The total dose was 60 Gy for the field radiotherapy (30 fractions of 2 Gy over six weeks). Teniposide at three dose levels (50 mg/m2, 75 mg/m2 and 100 mg/m2) was given intravenously once a week, totally five weeks. Dose escalation was based on each level, with a minimum of five patients in cohort if severe toxicity was not observed until the maximum tolerance dose(MTD). Results The predominant form of toxicity was hematologic toxicity. Four patients developed grade 3, 4 leucopenia when a second level of MTD (75 mg/m2) was given. Conclusion Combined radiotherapy and teniposide for postoperative brain neurospongioma is well tolerated. The dose of 50 mg/m2 for phase Ⅱ clinical trial was recommended.

[Objective] To determine the sun protection factor (SPF) and protection factor of ultraviolet A (PFA) of a self-made sunscreen cream.[Methods] The sample of a self-made sunscreen cream and a commercial sunscreen cream were painted to an artificial skin (3M film) at a dose of 2 μl/cm2,and a tester was used to evaluate their photoprotective effect.A total of 48 albinism guinea pigs were classified into 8 groups to remain unprotected or be protected by the self-made or commercial sunscreen cream.A solar ultraviolet light simulator (SUV-1000) was used to simulate the ultraviolet rays in sunlight to irradiate the guinea pigs,and the photoprotecfive effect of these sunscreen creams was determined.[Results] The in vitro evaluation revealed that the SPF value of the self-made sun-screen cream and commercial cream was 32.26 ± 2.42 and 30.87 ± 2.57respectively (n =5,t =0.94,P ＞ 0.05),and the PFA value was 24.28 ± 2.44 and 17.53 ± 2.28 respectively (n =5,t =4.52,P＜ 0.01 ).As the in vivo experiment showed,the SPF value of the self-made sun-screen cream and commercial cream was 30.39 ± 6.65 and 28.79 ± 7.36,respectively (n =12,t =0.38,P＞ 0.05),and the PFA value was ＞ 8.91 and ＞ 8.93 respectively.[Conclusion] The photoprotective effect of the self-made sunscreen cream is similar to that of the commercial cream.

AIM: To investigated the changes of hippocampus noradrenaline levels and its tyrosine hydroxyase (TH) , monoamine oxidase A (MAOA) expressions during chronic psycological stress procedure and physical exercise in rats. METHODS: Male Wistar rats (n =24) were randomly assigned to three conditions; (1) restraint stress (S) at 6 h/d for 3 weeks; (2) 24 h access to activity wheel running (E) for 4 weeks; (3) a sedentary control group (C). Levels of NE were assayed by high performance liquid chromatography with electrochemical detection. Protein expression of TH and MAOA were detected by Western blotting. RESULTS: Compared to C, weight loss and sucrose consumption rate decrease were induced in S, and NE was lower while TH and MAOA expressions increased significantly. In E group, NE was higher and TH, MAOA increased distinctly. CONCLUSION: It is suggested that hippocampus NE level may play an important role in the mechanism on the protection of physical exercise to chronic psycological stress, there is no close connection between NE change and TH, MAOA.Other mechanisms may exist.

AIM: This study is to determine changes of hippocampal norepinephrine (NE) and serotonin (5 -HT) in long term physical exercise and chronic psychological stress, and to study the roles of the two monoamine transmitters in the effect of exercise counteracting stress - induced hippocampal damages in brain. METHODS : Levels of hippocampal NE and 5 - HT in rats undergoing 4 - week voluntary wheel running exercise (exercise group) or 3 - week restraint stress (stress group) or 4 - week exercise and 3 - week stress (exercise - stress group) were detected by high - performance liquid chromatography using electrochemical detection. RESULTS: It is showed that levels of hippocampal NEand 5 - HT increased significantly (P < 0. 01) in the exercised rats, and in the stressed rats, hippocampal 5 - HT levelssignificantly decreased(P < 0. 05). Additionally, the NE levels maintained significant high (P < 0. 01) in exercise -stressed rats compared to the pure stressed ones. On the other hand, no obvious difference was observed in hippocampal5 - HT levels between stress group and exercise - stress group, which were all significant lower (P < 0. 05) than that in exercise group. CONCLUSION: It is suggested that both the NE and 5 - HT may play important roles in mediating the exercise-induced positive effects and the 5 - HT may play an important role in stress - induced negative effects on the hippocampus. Moreover, NE may take more action in the exercise attenuating stress - induced hippocampal damages. The hippocampal NE may be more susceptible to exercise, and the hippocampal 5 - HT may be more susceptible to stress.`

Objective To study the behavior factors associated with nonalcoholic fatty liver disease (NAFLD).Methods A total of 2038 subjects who underwent physical examination at the Department of Health check-up center,the Affiliated Hospital of Medical College,Qingdao University were included in this study(including 1438 cases of NAFLD).A questionnaire survey was conducted on their lifestyle and behaviors.Data of routine physical examination,ultrasound examination,liver function and blood lipid test were collected and analyzed.Results Multi-factors Logistic regression analysis showed that age (20 to 49 years) male sex,stress from work,sleep time, physical activity and diet axe among factors associated with NAFLD(all P＜0.05).Conclusion Lifestyle and behavior factor are associated with the incidence of NAFLD.