Mobile network is used to transmit signals for telemedicine dynamic ECG monitoring system.In this way,ECG monitoring can be performed to patients at all times and places,thus really realizing telemedicine dynamic ECG monitoring.In the paper,the system is studied.The discussion on how to transmit ECG signals and feedback the diagnostic message through mobile network is presented in detail.

Objective In order to investigate the changes of glycogen and lactate in cortex after exercise-induced central system fatigue. Methods Central fatigue model was established according to Bedford weight/maximal oxygen uptake equation. The rats were divided into three groups: control group (C), sedentary-exhausted group (SE), and overtraining group (O). Results After 1 week exercise, compared with control group, the weight of rats in SE and O group increased slowly. BUN of SE and O group increased significantly to 4.78?1.99 mmol/L and 8.48 ?0.92 mmol/L respectively,but Hb showed no changes. Compared with C group, both DA and DA/5-HT of O group decreased dynamical which were 309.04?63.68 ng/ml?g and 0.42?0.25 respectively. The latency of SEP in O group distinctly delayed, P1: 2.98?0.55ms, N1: 4.23?0.35ms, P2: 5.28?0.60ms. Regarding to 5-HT, no significant difference was found among all groups. Cortex lactate concentration of O (1.46mg/100g )and SE(1.12 mg/100g) group were significantly higher than control group(0.92 mg/100g). There was a tendency of glycogen decrease but had no significant difference among all groups. Conclusion The results suggested that lactate and glycogen might have close relationship with central fatigue and were the possible reasons to cause central fatigue.

BACKGROUND: The mechanism of central fatigue is unclear at present.The cumulation of lactic acid can play an important role in the central fatigue. Whether lactic acid can affect the neuronal functions, leading to the fatigue, needs to be examined.OBJECTIVE: To investigate the effects of lactic acid on the survival rate of primary neurons cultured from neonate rat cortex.DESIGN: Single sample observation SETTING: Laboratory of Cell Biology, Institute of Health and Environmental Medicine, Academy of Military Medical Science MATERIALS: Totally 40 Wistar neonate rats (within one day) provided by Experimental Animal Center of Academe of Military Medical Science were used in this study.METHODS: This experiment was conducted at the Laboratory of Cell Biology, Institute of Health and Environmental Medicine, Academy of Military Medical Science from October 2003 to May 2004. Primary neurons in added , and the pH value was decreased to 7.35,7.15,6.95 and 6.00,acid was added , and the pH value was kept at 7.35 , then the survival rate of neurons were observed. We analyzed the effect of lactic acid on neuron whether through down regulating pH value or the role of lactic acid.different concentrations of lactic acid and the same pH value on viability regulating pH value and lactic acid on neuron.pH. When PH values were 7.35,7.15,6.95 and 6.00, the survival rate of concentrations of lactic acid were 0,5,10 and 20 mmol/L,pH value was constantly kept at 7.35. Viabilities of neurons were 100%, 88%, 82%,81%, respectively, with significant difference as compared with control was significan tly lower than that of corresponding lactic acid group.CONCLUSION: A close relationship exists between pH decrease induced by lactic acid and viability of neurons. Excess lactic acid can have a toxic effect on neurons independently of t on the changes in levels of pH.

AIM: To explore the delayed protection of heme oxygenase-1 (HO-1) in the exercise preconditioning (EP) from the myocardial relative ischemia reperfusion injury (rI/R). METHODS: 40 Wistar Rats were divided into 5 groups randomly: control group (CN), rI/R group (IR), EP+rI/R group (EI), HO-1 inductor hemin+rI/R group (HE) and HO-1 inhibitor ZnPP+EP+rI/R group (EZ). The following indexes were detected, including the HO-1activity in myocardium, the cardiac function parameter-pressure-rate product (heart rate × left ventricular developed pressure, PRP) and the content of MDA in coronary effluent. RESULTS: After myocardial rI/R, HO-1 activity increased significantly. Moreover, EP or HO-1 inductor could enhance this effect manifestly. Nevertheless, when the HO-1 inhibitor was administered before EP,HO-1 activity decreased. In addition, there was no distinct difference in the HO-1 activity between EI group and HE group. At the 30 min point of reperfusion, the PRP recovery rate of EI group was higher clearly than that of IR group. However, there was reverse effect between the EZ group and the EI group. The MDA in coronary effluent of EI group, EZ group and HE group were lower obviously than that of IR group and there was significant difference between EI group and EZ group. CONCLUSION: EP could protect the heart from the rI/R injury occurring 24 hours later, which might be performed through activating the HO-1.

Objective:To investigate the duck thymocyte surface antigens.Methods:Repeated immunization of normal BALB/c mice with separated thymocyte from mature duck result in the production of mAbs which react with duck thymocyte surface antigens.Applying the immunohistochemistry assay,tracing the originating and distributing of lymphocytes which reacted with mAb in spleen,thymus,and bursa of duck different development stages.Results:Five monoclonal antibody-producing hybridomas(B6,B8,C12,E6,H5)were isolated.According to the result of immunohistochemistry assay,there were distributing strained lymphocytes in thymus,bursa and spleen of duck.The stained cells belonged to different populations.Conclusion:The mAb C12 reacted with the common cell surface marker of between mature T lymphocytes and dendritic cells.

AIM: To investigated the changes of hippocampus noradrenaline levels and its tyrosine hydroxyase (TH) , monoamine oxidase A (MAOA) expressions during chronic psycological stress procedure and physical exercise in rats. METHODS: Male Wistar rats (n =24) were randomly assigned to three conditions; (1) restraint stress (S) at 6 h/d for 3 weeks; (2) 24 h access to activity wheel running (E) for 4 weeks; (3) a sedentary control group (C). Levels of NE were assayed by high performance liquid chromatography with electrochemical detection. Protein expression of TH and MAOA were detected by Western blotting. RESULTS: Compared to C, weight loss and sucrose consumption rate decrease were induced in S, and NE was lower while TH and MAOA expressions increased significantly. In E group, NE was higher and TH, MAOA increased distinctly. CONCLUSION: It is suggested that hippocampus NE level may play an important role in the mechanism on the protection of physical exercise to chronic psycological stress, there is no close connection between NE change and TH, MAOA.Other mechanisms may exist.

ABSTRACT:Objective To investigate the differential gene expression of nasopharyngeal carcinoma cell line CNE-2 and CNE-2R exposed to X-rays.Methods We used neutral comet assay to detect the intrinsic radiosensitiv-ity of tumor cells,immunofluorescence to detect the phosphorylated histone γH2AX and Rad5 1 foci formation and to assay the time-dose effects on cell radiation injury and radiation radiosensitivity change after irradiation.The dif-ferential gene expression was screened with cDNA microarray by DNA damage repair related gene chip.Differenti-ally expressed proteins screened were confirmed by Western blot.Results After 2 h of 4 Gy 9MeV-β-ray irradia-tion,CNE-2R cells had aggravated DNA damage compared to CNE-2 cells and the damage was more obvious with the prolongation of time.It suggested that cell DNA damage severity was more obvious.The immunofluorescence showed the positive rates ofγH2AX cells in CNE-2 cell line were higher than those in CNE-2R cell line.There were 37 different genes related to DNA damage repair in CNE-2 cell line compared with CNE-2R cell line,while 24 up-regulated and 13 down-regulated genes were found in SiHaR cells.In them,there were 6 genes whose ratios were 6 times higher and 3 genes whose ratios were lower than 0.1.Western blot verified the results of four differentially expressed proteins compared with CNE-2 cells,in which GADD45a and RRAD1 expression levels were significantly lowered in CNE-2R while the RAD9a and RCF2 protein significantly increased.Conclusion The radioresistance of CNE-2R cells is significantly related to DNA damage repair capacity in certain mutations at the gene level.It is possible to regulate cell radiosensitivity by regulating the related gene.

Objective To observe the clinical efficacy of glucocorticoidcombined with the eradication of helicobactepylori (HP) in the treatmenof idiopathithrombocytopenipurpura(ITP) and itinfluence on platelecounand cell subset.MethodNinety-foucaseof ITP were randomly divided into two group.The control group(47 cases) wagiven prednisone treatmen, while the observation group(47 cases) wagiven prednisone combined with HP eradication treatmen.The clinical effecand recur-rence rate within 1 yeawere observed ,and the changeof platelecounand cell subsetwere tested .ResultThe total effective rate of the observation group wa89 .36% ,which wasignificantly highethan 70 .21% in the control group ;the 1-yearecurrence rate in the observation group wa36 .17% ,which wasignificantly lowethan 72 .34% in the control group ,the differencebetween the two grouphad statistical significance (P＜0 .05) .The platelecounaftetreatmenin the two groupwere significantly in-creased ,platelet-associated antibody(PAIgG) level wasignificantly decreased ,buthe improvemenin the observation group wamore significant(P＜0 .05) .The percentage of CD3+CD4+ % increased significantly ,the percentage of CD3+CD8+ % wadecreased significantly ,buthe improvemenin the observation group wamore obviou(P＜0 .05) .Conclusion Glucocorticoidcombined with HP eradication treatmenin treating ITP hadefinitely clinical efficacy ,can increase the platelecoun,improve cell subsetimbalance ,and iworthy to be clinically promoted .

AIM: This study is to determine changes of hippocampal norepinephrine (NE) and serotonin (5 -HT) in long term physical exercise and chronic psychological stress, and to study the roles of the two monoamine transmitters in the effect of exercise counteracting stress - induced hippocampal damages in brain. METHODS : Levels of hippocampal NE and 5 - HT in rats undergoing 4 - week voluntary wheel running exercise (exercise group) or 3 - week restraint stress (stress group) or 4 - week exercise and 3 - week stress (exercise - stress group) were detected by high - performance liquid chromatography using electrochemical detection. RESULTS: It is showed that levels of hippocampal NEand 5 - HT increased significantly (P < 0. 01) in the exercised rats, and in the stressed rats, hippocampal 5 - HT levelssignificantly decreased(P < 0. 05). Additionally, the NE levels maintained significant high (P < 0. 01) in exercise -stressed rats compared to the pure stressed ones. On the other hand, no obvious difference was observed in hippocampal5 - HT levels between stress group and exercise - stress group, which were all significant lower (P < 0. 05) than that in exercise group. CONCLUSION: It is suggested that both the NE and 5 - HT may play important roles in mediating the exercise-induced positive effects and the 5 - HT may play an important role in stress - induced negative effects on the hippocampus. Moreover, NE may take more action in the exercise attenuating stress - induced hippocampal damages. The hippocampal NE may be more susceptible to exercise, and the hippocampal 5 - HT may be more susceptible to stress.`

Objective To study the behavior factors associated with nonalcoholic fatty liver disease (NAFLD).Methods A total of 2038 subjects who underwent physical examination at the Department of Health check-up center,the Affiliated Hospital of Medical College,Qingdao University were included in this study(including 1438 cases of NAFLD).A questionnaire survey was conducted on their lifestyle and behaviors.Data of routine physical examination,ultrasound examination,liver function and blood lipid test were collected and analyzed.Results Multi-factors Logistic regression analysis showed that age (20 to 49 years) male sex,stress from work,sleep time, physical activity and diet axe among factors associated with NAFLD(all P＜0.05).Conclusion Lifestyle and behavior factor are associated with the incidence of NAFLD.