Clinical and epidemiological studies have shown that inflammatory bowel diseases (IBD) can increase the risks of cerebral artery and venous thrombosis.The pathogenesis of cerebrovascular disease in IBD remains unclear.IBD itself and its accompanying hypercoagulable state may be the main reasons.Clinicians familiar with neurological manifestations of IBD,early initiation of risk assessment for thromboembolism,and timely multidisciplinary consultation are very important for early diagnosis.The inpatients with IBD should receive the active measures of thromboprophylaxis.Usually,the preventive use of low-molecular-weight heparin is safe for patients at high risk of thrombosis.The treatment scheme for IBD patients with cerebral infarction and cerebral venous thrombosis is the same as other cerebrovascular diseases,but the most critical is that the treatment and remission of IBD itself.In case of emergency,intraarterial local thrombolysis or mechanical thrombectomy can be used;however,any benefits should be weighed against the high risk of intestinal bleeding.

Compared with the stiff traditional teaching mode,the application of problem-based learning(PBL)mode to education reform in sanitary chemistry owns great advantages of stimulating study-motive formation and improving practical ability.In practice,problem design,self-study guidance,proposal implement and feedback should be involved in the whole procedure.

ObjectiveTo prepare varicella-zoster virus(VZV)harvest and immunize Japanese rabbits with purified bulk of virus to prepare antiserum against VZV,and to perform various controltests.MethodsThe VZV Oka strain(vOka)was subcultured in human diploid cell line 2BS. The harvested virus was purified by ultracentrifugation to prepare immunogen,which was mixed with Freund′s adjuvant and then used to immunize 5 female Japanese white rabbits by multi-point subcutaneous injection to prepare VZV antiserum. The specificity of serum antibody was detected by Western blot. The titers of fluorescent antibody to membrane antigen(FAMA)and serum neutralizing antibody were determined,and the correlation between the results of the two detection methods was analyzed. Interference of heterogenous viruses was performed on measles,mumps and rubella viruses by using antiserum with high antibody titer.ResultsThe prepared VZV antiserum had specific binding with vOka and Oka-7S virus harvests,gE and ORF7 proteins and other VZV antigens. After the third immunization,the serum antibody titer detected by FAMA was 1 ∶ 16 384,and the neutralizing antibody titer was 1 ∶ 256.According to Karl Pearson correlation coefficient analysis,the results of the two detection methods were linearly correlated. Interference of heterogenous viruses showed that the differences between the experimental and control groups were less than 0. 50 lgCCID50/mL,indicating that the antisera against VZV had no interference to the titers of measles,mumps and rubella viruses.ConclusionThe VZV antiserum with high specificity and affinity was successfully prepared,which lays a foundation for the validation of VZV component vaccines.

Objective To culture normal adult esophageal epithelial cells and establish a normal esophageal epithelial cell line to provide experimental materials for further studies in vitro.Methods Normal esophageal epithelium samples were obtained in sterile phosphate buffered saline supplemented with antibiotics from the normal part of the esophagus of a patient with esophageal carcinoma.Tissue was cleaned of all connective tissues and muscles and rinsed in phosphate buffered saline 5-6 times.The mucosal layer was minced into small pieces and dissociated into a single cell suspension by 0.25% Dispase and 0.25% trypsin/0.02%EDTA.Cells were grown in keratinocyte serum-free media.The cultured cells were identified through their morphological characteristics and immunohistochemical staining.The proliferative capacity of the cultured cells was also examined.Results The cultured cells showed microscopic features of epithelial cells and were positive in keratin and epithelial membrane antigen staining.Eight days after primary culture,the cells displayed a cobblestone morphology reaching 80%-90% confluency and were passaged successfully with 0.25% trypsin/0.02%EDTA.The cell cycle analysis showed about 77.60% of the cultured cells was in G0/G1 phase and the others in S/G2/M phase.Conclusion\ The culture methods and techniques used in the experiments are convenient and suitable for the primary culture and subculture of normal adult esophageal epithelial cells.

Objective To observe the attachment,shape,function and activity of human umbilical cord mesenchymal stem cells cultured on silk fibroin porous scaffolds in vitro,and to provide experimental foundation for the choice of scaffold in the study of adipocyte tissue engineering.Methods These silk fibroin porous scaffolds were subsequently seeded with hUCMSCs and cultured in vitro.The growth and function of hUCMSCs were observed and measured with fluorescence inverted microscopy,scanning electronic microscopy and MTT methed. Results hUCMSCs were fixed on silk fibroin porous scaffolds 1 or 2 days later,and multiplicative growth was observed on the 5th to 7th day.After about 10 days,the microholes of the scaffolds were overlayed by hUCMSCs.Scanning electronic microscopy and fluorescence inverted microscopy showed that cells adhered to scafold well and there was a lot of extra cellular matrix surrounding cells. Conclusion Silk fibroin porous scaffolds are ideal for attachment,growth,function maintenance and activity of hUCMSCs.and the scaffolds can be used as natural scaffolds for hUCMSCs in 3D culture.

The rapid recovery of postoperative gastrointestinal motility is the key factor to accelerate the recovery of patients. The recovery of gastrointestinal motility is often affected by various factors, such as anesthesia and operation. The present study shows that the inhibition of stress response caused by acute pain can improve the recovery of gastrointestinal motility. Howeve, the effects of perioperative intravenous analgesics on gastrointestinal motility are not consistent. So we reviewed the effects of different intravenous analgesics on gastrointestinal motility.

AIM:To study the effects of different duration of sleep deprivation (SD) on neurogranin (Ng),protein kinase C (PKC) and Ca2+-calmodulin dependent protein kinase II (CaMK II) mRNA expressions in hippocampus and forebrain of rats. METHODS:Male Wistar rats were divided into three groups:24 h,48 h and 72 h experimental groups. Each group was divided into sleep deprivation group (SD group) and control group (C group). SD model was established by sleep deprivation box. One step methods by Trizol was used to extract hippocampus and forebrain neuronal total RNA. The changes of Ng,PKC and CaMK II mRNA expressions were detected by SYBRA green I RT-PCR. RESULTS:(1) The expression of Ng mRNA in hippocampus of SD group was significantly lower than that in C group after 24 h,48 h and 72 h SD (P

Objective To investigate the influence of calcium channel blockers on intracellular calcium levels of cold preservation-reperfusion injury during liver transplantation in rats.Methods According to the ingredient of perfusion and preservation solutions,the animals were divided into four groups: the control group with HCA solution,the experimental group I with HCA solution plus Verapamil,the experimental group II with HCA solution plus Nifedipine and experimental group III with HCA solution plus Diltiazem.The intracellular calcium of the rat hepatocytes and liver function were measured and the liver structure was observed under light and electron microscopy.Results There were significant differences between the experimental groups and the control group in intracellular calcium concentration and liver function(P

The internal limiting membrane (ILM),composed of collagen fibers,glycosaminoglycans,laminin and fibronectin,is the basement membrane of the retinal Müller glia cells and serves as an interface between the vitreous and retina.The ILM is the structural interface between the vitreous and retina.ILM removal ensures separation of the posterior hyaloid from the macular surface,which can relieve macular traction and prevent postoperative epiretinal membrane formation.Thus,vitrectomy with ILM peeling has become an increasingly utilized and vital component in surgical intervention for various vitreoretinal disorders.However,many recent studies showed that ILM peeling is a procedure that can cause immediate traumatic effects and progressive modification on the underlying inner retinal layers.There were some surgical strategy (fovea-sparing ILM peeling or inverted internal limiting membrane flap technique,or Abrasion Technique).But some controversies exist,such as when ILM peeling is necessary,which adjuvant to use to perform the procedure,and what is the best technique to peel the ILM.A full assessment ILM structure and function and related factors of surgery is helpful to predict the anatomical and functional prognosis.

Objective To investigates the diagnostic value of CT of the gallbladder and accuracy.Methods Clinical data of 26 patients with pathologically confirmed gallhladder were retrospectively analyzed.Results 26 patients were diagnosed with CT.CT manifestations of primary gallbladder carcinoma:papillary intraluminal mass type,limited or diffuse gallbladder wall thickening,large cystic mass in full or swallowed type.Combined with the direct signs of pathology gallbladder cancer was divided into three types:infiltrative,nodular,tumor type;Enhance a performance of CT scan of gallbladder:artery of high density,such as density of the portal vein,followed by arterial and portal vein of both high-density,higher than normal latency of the gallbladder wall and liver,regression not significant,especially gallbladder junction local thickening of the gallbladder wall continued enhanced features.Conclusion The performance of primary gallbladder was complex,and CT examination was a key means of establishing diagnosis.