Objective:To investigate the expression of MMP-13 in breast cancer tissues and its relationship with clinical prognosis of patients.Methods: Tissue microarray and immunohistochemical SP method were used to detect the expression of MMP-13 in 183 specimens of breast cancer.The association between the expression of MMP-13 and the clinicopathologic parameters of patients was analyzed;the association between MMP-13 and the prognosis of breast cancer was also discussed based on 10 years' follow-up data.Results: The positive rate of MMP-13 in the breast cancer tissue(54.1%) was significantly higher than that in the benign breast tissues(13.3%,P

Objective To detect the expression of miRNA‐29a in abdominal aortic aneurysm(AAA) patients and construct a recombinant lentiviral vector carrying miRNA‐29a ,transfection of human abdominal aortic vascular smooth muscle cells (HVSMCs) ,and to investigate the alteration on the proliferation in HVSMCs .Methods Collected 25 AAA patients′serum and 25 normal elderly serum specimens from March 2011 to March 2014 ,the mean age were 62 .9 ± 13 .6 and 61 .5 ± 11 .8 separately .Em‐ploy RT‐PCR analysis the miRNA‐29 expression in different group .The MCS‐CMV‐miRNA‐29a was transfected into HVSMCs cells ,the transfection efficiency was observed with fluorescence microscope ,the mRNA and COL1A1 protein expresstion was detec‐ted by RT‐PCR and Western blot respectively .MTS method was used to determine effects of cell inhibition rates of different groups ,cell apoptosis was analysised by Annexin V/7‐AAD double staining after 72 h .Results The expression of miRNA‐29a with AAA was lower than normal group .The transfection efficiency was (74 .25 ± 10 .10)% .miRNA‐29a mRNA and its target protein COL1A1 expression increased significantly compared with the control group after transfection ,MTS and Annexin V/7‐AAD results showed that cell apoptosis was decreased after miRNA‐29a overexpression ,cell proliferation was increased compared with the con‐trol group .Conclusion The miRNA‐29 expression decreased associated with the occurrence of abdominal aortic aneurysm ,but overexpress miRNA‐29a in HVSMC cells by lentivirus mediated ,which could promote the proliferation of cells ,inhibit its apoptosis .

Objective To compare the influence of two different managements of relief incision (exposed relaxation therapy or absorbable hemostatic gauze) in infants' palatoplasty. Methods A total of 118 infants with cleft palate between 5- to 8-month-old for the study were randomly divided into two groups: group A was exposed therapy group, and in group B both sides of relief incision were placed absorbable hemostatic gauze. The wound bleeding, body temperature, diet and incidence of fistula were observed and analyzed after operation. Results There was no significant difference between the two groups in postoperative temperature, diet and wound healing (P>0. 05). The postoperative wound bleeding was statistically significant differences between the two groups (P<0. 05). Conclusions Either exposed therapy or placed absorbable hemostatic gauze in relief incision, has minimal systemic and local reactions in the postoperative infant. The advantages of exposed treatment are economic and simple, and less irritant to the wound.

Objective To analyze the m edical records of poisoned children to provide references for the forensic identification ofmelam ine-tainted milk pow der poisoning. Methods M edical records of six fatal cases of consum ing som e brandmelam ine-tainted milk pow der w ere studied, specifically the poisoning sym ptom s, m edical im aging, blood biochem ical tests, treatm ent and prognosis. Results The m ajor m edical problems of these eight-m onth sick infants w ere urinary tract obstruction caused by urinary tract calculi. The poisoned infants developed oliguria, anuria and other sym ptom s, eventually, acute renal failure or other com plications leaded to death. The serum BUN and Crabnorm ally increased. Conclusion B y con-sidering the toxicological effects of melam ine, it was concluded that the deaths of these sick infants w ere related to themelam ine poisoning.

Objective:To study the morphology and function of the dendritic cells(DC) from human peripheral blood monocytes and the change in cell differentiation and maturation after DC treated with dexamethasone(Dex-DC).Methods:DCs were sorted from human monocytes by culturing in presence of cytokines(GM-CSF, IL-4) and LPS for 8 days with or without dexamethasone. The change in morphology, function and phenotypic characterization was compared.Results:The cells treated with or without Dex, developed a characteristic dendritic morphology, however, Dex-DC expressed higher level of CD14 and lower level of CD83 than the untreated cells. In addition, a low APC function was demonstrated by Dex-DC.Conclusion:DCs can be induced from peripheral blood monocytes in medium with cytokines; the cultured PS in presence of Dex are shown at a more immature stage, indicating that Dex modulates DC differentiation, maturation, and function.

Objective To investigate the improving effect of betahistine hydrochloride (BH) on the cochlear blood supply in guinea pig and the potential use in cochlear ischemia. Methods The guinea pig model of ischemic inner ear was established by ferromagnetic embolization of the target microvessels of the cochlea. A total of 10 model guinea pigs were divided into BH group and control group ( n =5 in each group). Guinea pigs in BH group were treated with 0.1% BH through gastric canal, and those in the control group with the identical volume of saline. At 24 h after treatment, the cochlear blood flow (CBF), auditory brainstem responses (ABR), ferromagnetic emboli in the microvessels of cochlear lateral wall, and the hair cell succinic dehydrogenase (SDH) activity were detected. Results CBF in 10 ears in the control group was (50?7)%, and the elevated ABR threshold was found in 7 ears (70%). Ferromagnetic emboli confirmed by carbonyl iron spheres were extensively distributed in the microvessels in the cochlear lateral wall. Spot like lesion of the outer hair cells was found in SDH stained basic membrane. However, in BH group, CBF was (81?11)%. Elevated threshold of ABR was found in only 3 ears, and the ferromagnetic emboli in the microvessles in the cochlear lateral wall were less than those in the control group. Scattered lesions of the outer hair cells were found in SDH stained basic membrane. There was significant difference in the number of deleted hair cells between the BH group and the control group. Conclusion The preliminary results indicated that BH may increase CBF and attenuate the cochlear lesion due to ischemia, resulting in partial protection of hearing.

Objective To investigate the influence of calcium channel blockers on intracellular calcium levels of cold preservation-reperfusion injury during liver transplantation in rats.Methods According to the ingredient of perfusion and preservation solutions,the animals were divided into four groups: the control group with HCA solution,the experimental group I with HCA solution plus Verapamil,the experimental group II with HCA solution plus Nifedipine and experimental group III with HCA solution plus Diltiazem.The intracellular calcium of the rat hepatocytes and liver function were measured and the liver structure was observed under light and electron microscopy.Results There were significant differences between the experimental groups and the control group in intracellular calcium concentration and liver function(P

This paper underlines the composition of OS(Quorum Sensing) systems in Pesudomonas aeruginosa,the resistance mechanism of Pesudomonas aeruginosa and the relevance between QS systems and the resistance mechanism.What's more,it introduces several pathways of the interfering of QS systems to weaken the resistance.These will show a new prospect to the control of bacterial infection.

Objective To investigate the effects and mechanism of dexmedetomidine hydrochloride preconditioning against glutamate-induced neurotoxicity.Methods The model of glutamate-induced neurotoxicity was established by the injection of glutamate into lateral cerebral ventricle.Thirty-six SD rats were randomly divided into control group (C group),glutamate-induced neurotoxicity group (G group),Dex1 group and Dex2 group.Dex1 group and Dex2 group received intraperitoneal injection of dexmedetomidine respectively at a dose of 50 μg/kg or 100 μg/kg before glutamate application.Two hours later,the rats were sacrificed and hippocampus was separated to measure the level of SOD and MDA.The rest of each brain was used to measure the degree of brain edema.Pathological changes were observed under microscope with Nissl's staining.Results In contrast to G group,brain edema and MDA concentration in Dex1 group and Dex2 group were significant lower,while SOD concentrations were significantly increased and the pathological change in Dex1 group and Dex2 group were relieved obviously compared to glutamate-induced neurotoxicity group.Conclusion Dexmedetomidine preconditioning can significantly attenuate glutamate-induced neurotoxicity,which is properly related to the inhibition of oxidative-stress reaction.

Objective To study the apoptosis-inducing effect of Oridonin on PC-3 cells line and the role of Survivin in the process.Methods After PC-3 cells were incubated with different concentrations of Oridonin,cell viability was analyzed with MTT assay.The percentage of earlier apoptosis cell was analyzed by flow cytometry.The protein expression of Survivin in PC-3 cells were detected by Western blot and fluorescent quantitative PCR.Results Oridonin effectively inhibited the proliferation of PC-3 cells in a concentration-time dependent way.After PC-3 cells were treated with Oridonin ( 2.5,5,10,20,40 μmol/L)for 48 hours,the cytotoxicity index were 9.2％,25.3％,39.3％,77.2％,92.5％ and the IC50 of PC-3 cells was 10.29 μmol/L,respectively.Flow cytometry was used to detect the effect of different concentration of Oridonin (0,10,20,40 μmol/L) for 48 hours,the apoptotic rates of PC-3 cells were 4.8％,15.4％,19.5％,27.4％ ( P ＜ 0.05).Oridonin down-regulated Survivin protein in a concentration-dependent way in PC-3 cells.Conclusions Oridonin can induce the apoptosis of PC-3 cells by a concentration-dependent manner.Oridonin can induce the apoptosis of PC-3 cells by down-regulated Survivin protein.