By means of TLC-densitomery, the contents of oleanolic acid extracted from the fruits of Ligustrum lucidum were determined to be 8.04% (g/g) in the unriped fruit collected in August and 2.73% (g/g) in riped fruit gathered in December. This result showed that the oleanolic acid content in fructus L. Iucidum decreases gradually with growing until the fruit is fully riped.

Objectives: To study the clinical meaning of condyle-form asymmetry and its osseous changes shown on TMJ images of lateral oblique transcranial projection and lateral tomography. Methods: 113 patients with partial posterior teeth lose were enroled in the study.Among them 25 were with temporomandibular disorder (TMD group),39 with slight TMD sings (sub-TMD group) and 45 without TMD(TMD-free group). Radiographs were taken and studied for each patient. Results: It was found that the condyle form asymmetry and osseous changes were not significantly different among the three groups. Osseous change was related to the number of teeth-lose (P0.05). The symptoms and signs of TMD had close relationship with occlusal interferences. Conclusions: The changes shown on TMJ images do not show close relationship with TMD symptoms and signs. Occlusal interferences resulted from teeth-lose are related to the clinical symptoms and signs of TMD.

Objective To investigate the relationship between expressions of alpha 2-HS-glycoprotein (AHSG) gene mRNA and protein in placenta and insulin resistance in patients with gestational diabetes mellitus (GDM).Methods The expressions of AHSG gene mRNA and protein in placenta were detected by reverse transcriptase-polymerase chain reaction (RT-PC R) and Western blot technique in normal uterogestation uterine-incision delivery patients (control group,43 cases) and patients with GDM (GDM group,45 cases).Fasting plasma glucose (FPG) and fasting insuhn (FINS) were detected before uterine-incision delivery,and the homeostasis model of assessment for insulin resistance index (HOMA-IR) was computed.Results The FPG,FINS and HOMA-IR in GDM group were significantly higher than those in control group [(5.28 ± 0.63)mmol/L vs.(4.24 ±0.21) mmol/L,(11.75 ±4.85) mU/L vs.(9.73 ±2.18) mU/L,2.79 ±1.02 vs.1.87 ± 0.66],there were statistical differences (P ＜ 0.01 or ＜ 0.05).The expressions of AHSG gene mRNA and protein in GDM group were significantly higher than those in control group (0.761 6 ± 0.069 1 vs.0.329 4 ± 0.025 1 and 0.824 5 ± 0.137 5 vs.0.420 3 ± 0.075 0),there were statistical differences (P＜ 0.01or ＜ 0.05).HOMA-IR level in GDM group was positively correlated with AHSG gene mRNA and protein (r =0.633,0.587,P ＜0.05).Conclusions The expressions of AHSG gene mRNA and protein are significantly higher in patients with GDM.Insulin resistance may play an important role in the pathogenesis and development of GDM.

Objective To explore the role of caspase-3 activation and DNA fragmentation in later period of neonatal rat hypoxic-ischemic brain damage(HIBD). Methods DNA fragmentation,caspase-3 mRNA and caspase-3 protein were measured in 2 wks、3 wks and 4wks after setting HIBD animal model in newborn wistar rats by FCM, RT-PCR and Immunohistochemistry. Results (1) Apoptosis lasted 4ks after HIBD. This suggested a long lasting role of apoptosis in neonatal HIBD by TNNEL and EM.(2)Caspase-3 mRNA expression was estimated by semi-quantitative RT-PCR. It was higher in HIBD group(0.771?0.074) than in control group(0.620?0.038, P0.05. Average Avalue of Caspase-3 protein in HIBD group(0.374 ?0.038) at 3 wks was significantly higher than that in control group(0.356?0.020,P

Objectives: To investigate the effects of cent ra l disordered occlusion on the thickness of TMJ disc. Methods:The thickness of temporomandibular joint disc was measured in 5 cadavers with centr al disordered occlusion, including secondary malocclusion caused by loss of post erior tooth for a comparable long time with collapse of neighbor teeth and supra -eruption of its antagonized tooth, the supra-erupted third molar resulted fro m lack of antagonized tooth for a comparable long time and individual posterior tooth cross bite or reverse cross bite asymmetrically. Other 7 cadavers without central disordered occlusion served as controls. All samples were examined histo logically for the TMJ disc thickness. Out of 18 New Zealand rabbits, aged 5 mont hs, 9 were treated orthodontically to pull the first premolar move medially, occ luding with its opposite un-coincidently, central disordered occlusion was grad ually induced. The other 9 rabbits served as blank controls. The TMJs were sampl ed 3 months after beginning of the experiments, stained with HE and observed und er microscope. Results: In both cadavers and animals, the TMJ di sc was found much thicker in central disordered occlusion group than that in the control group (P

Purpose:To explore the value of MSCT-3D in stomatology through comparing with COPT.Materials and Methods:Both MSCT 117 patients and COPT 117 patients in stomatology were selected at a particular period,COPT imaging was using computed post-processing,MSCT imaging was processed according to 3D information through work station,then to analyze the imaging results according to metal tooth、damaged tooth and broken tooth,later to compare the photograph 、the technique of computed post-processing、the photograph condition and exposure of MSCT and COPT.Results:Normal COPT patients were 9 cases ( 0.24% ),normal teeth were 3160 (84.4% ),unusual teeth were 551 (15.6%),normal MSCT-3D patients were llcases ( 0.29% ),normal teeth were 3138 (83.8% ),and unusual teeth were 606 (16.2% ).COPT belonged to COPT photograph,the emphasis was 2D,MSCT-3D belonged to spiral photograph,the emphasis was 3D,and MSCT imaging could be processed according to 3D reformation.The 3D effect of MSCT was batter than that of COPT.The radiation dosage was much higher than that of COPT.Conclusions: COPT could get 2D effects: MSCT-3D could improve 3D results on 2D basis,but the radiation dosage of MSCT is much higher than that of COPT in stomatology.

Objective To investigate the effect of microRNA (miRNA )‐548ah targeting histone deacetylase‐4 (HDAC4) on the replication and expression of hepatitis B virus (HBV) .Methods HepG2 , 2 ,15 cells were transfected by mimics and inhibitors . The expressions of miRNA‐548ah and HDAC4 before and after transfection were detected by fluorescent quantitative polymerase chain reaction (PCR) . The expression of HDAC4 protein in HepG2 ,2 ,15 cells was detected by Western blotting .The target gene of miRNA‐548ah was analyzed by bioinformatics methods .3′UTR dual‐luciferase expression vector containing candidate HDAC4 target genes was built to test the luciferase activity .The levels of hepatitis B e antigen (HBeAg) and hepatitis B surface antigen (HBsAg) in the supernatant of cultured HepG2 ,2 ,15 cells were detected by enzyme‐linked immunosorbent assay (ELISA) .The level of HBV DNA in the supernatant of HepG2 ,2 ,15 cells was detected by fluorescent quantitative PCR .The t‐test was used for comparison between two groups ,SNK‐q tests were used for multiple groups comparisons .Results The expressions of miRNA‐548ah in HepG2 ,2 ,15 and HepG2 cells were 5 .74 ± 0 .02 and 2 .96 ± 0 .40 , respectively (t= 11 .89 ,P< 0 .01) ,and the expressions of HDAC4 mRNA were 9 .38 ± 0 .39 and 18 .13 ±0 .34 ,respectively (t = 29 .39 , P < 0 .01) . The expression of miRNA‐548ah in HepG2 ,2 ,15 cells was inhibited by transfection of miRNA‐548ah inhibitors (1 .01 ± 0 .13 ,t= 15 .48 , P< 0 .01) .Compared with control group ,the levels of HBsAg ([6 .45 ± 0 .46 ] IU/mL vs [2 .60 ± 0 .20 ] IU/mL , t = 7 .48 , P <0 .01) ,HBeAg ([5 .49 ± 0 .27] NCU/mL vs [4 .15 ± 0 .34 ] NCU/mL , t = 3 .10 , P < 0 .05 ) and HBV DNA ([3 .93 ± 0 .06] lg copy/mL vs[2 .04 ± 0 .07] lg copy/mL ,t = 18 .89 , P< 0 .01) in the supernatant of cultured HepG2 ,2 ,15 cells significantly decreased in inhibitor group . The expression of HDAC4 in HepG2 ,2 ,15 cells significantly decreased after transfection of miRNA‐548ah mimics (2 .98 ± 0 .94) ,but significantly increased after transfection of miRNA‐548ah inhibitors (23 .77 ± 6 .74 ) , with statistical significance (F= 9 .34 , P< 0 .01) .The expression of HDAC4 protein was also significantly inhibited after transfection of miRNA‐548ah mimics (0 .53 ± 0 .14 vs 0 .23 ± 0 .02 , t = 3 .58 , P = 0 .02) .The activity of luciferase was significantly inhibited by transfection of miRNA‐548ah mimics (7 .62 ± 0 .45 vs 6 .65 ±0 .27 ,t = 3 .18 , P = 0 .03 ) .Conclusion miRNA‐548ah may promote the replication and expression of HBV through the regulation of target gene HDAC4 .

This article introduces a constant-temperature-and-humidity test box, which, with a high-performance Aduc812 singlechip as the core, is composed of an integrated temperature and humidity sensor, a serial keyboard controller and a lattice-style liquid crystal module. With balance control mode of temperature an d humidity, PID control, temperature and humidity decoupling applied, the high-r esponse-speed and high-precision control can be fulfilled.

OBJECTIVE To assure the medical safety,enhance the medical care,prevent medical infection by(endoscope) examination and do effectively the standardized management for rinse and disinfection of endoscope.METHODS Strengthening the organization,to establish and consummate the system,increase the devotion of(facilities,) organize personal training and standardize the work procedures.RESULTS The work for rinse and(disinfection) of endoscope in hospital came to be standardized management gradually.CONCLUSIONS The(standardized) management for rinse and disinfection of endoscope is the assurance for the validity and security of(endoscope) sterilization.

OBJECTIVE To enhance the management against the HIV and AIDS,strengthen the awareness of occupational protection among medical staff,and prevent the HIV spreads in the hospital.METHODS To establish the HIV/AIDS monitoring report management system,launching AIDS knowledge training,screening HIV seriously in hospital,sterilizing strictly with the medical instrument and managing the medical discards,and standardizing procedures of the medical nursing and process after the occupational exposing.RESULTS Through establishing the HIV/AIDS monitoring report management system and launching occupation protection training,the awareness of medical staff for precaution against the HIV/ AIDS was enhanced obviously.They could discover the patients early and take the effective measures for protection,sterilization and isolation in time.The average number of diagnosis days for HIV/AIDS patients shortened obviously than before.CONCLUSIONS Through enhancing management and supervision for HIV/AIDS in hospital,paying more attention to safety education for medical staff,we can prevent the AIDS spreading in the hospital and reduce the occurrence of occupational exposure among medical staff.