The CFU-GM in 30 full-term newborn cord blood were cultured on the mon-olayer agar and compared with those,in quatity and quality,of cord blood,adultbone marrow and peripheral blood,indicating:1.The CFU-GM in newborn cordblood were in a state of the active proliferization with characterization of rapiddivision,early colony appearance,largy colony volume and more filial cell con-tainment;2.The yield of CFU-GM and cluster in full-term newborn cord blo-od ranged between those in the adult bone marrow and peripheral blood but variedgreatly from one individual to another,the yield of CFU-GM in 13 3% cordblood samples was higher than that in the bone marrow cultured at the same time.In addition,the colony type of CFU-GM in cord blood,adult bone marrow andperipheral blood was different,suggesting that the CFU-GM in cord blood mightbe a different subtype.

The amount of CFU-GM colony and cluster yielded after the two units of cord blood were mixed, was observed,using the in vitro CFU-GM culture technique, indicating that the yield of CFU-GM colony and cluster in the mixed culture group was no less than that in the single culture group, so this study can provide the theoritical basis for the transplantation of mixed cord blood hematopoietic stem cells.

Objective To observe the effect of Danshen Yin on some gastric mucosal defensive factors in rats with acetic acid-induced gastric ulcer and to explore its anti-ulcer mechanisms.Methods Rat models with acetic acid-induced gastric ulcer were treated with Danshen Yin,ranitidine,and saline respectively for 14 days. After treatment,the ulcer index,serum nitric oxide(NO) level,plasma prostaglandin E2(PGE2)content and the number of micrangium in the granulation at the ulcer base were examined.Results The ulcer index in Dandhen Yin group and ranitidine group was significantly less than that in model group(P

The emulsifiers of the ferula oil was shown to suppress markedly the swelling of the hind paw induced by injection of carrageenin and to suppress the increase of vascular permeability induced by intrad-ermal HA or 5-HT in rats. It also showed significant suppressive effects on oedema of rats hind paw induced by CFA.Experimental results indicated that it has inhibiting effect on DTH by SRBC in mice and suppressing on lymphocytes transformation induced by PHA.

AIM: To evaluate the effect of polysaccharide from spirulina platensis (PSP) on hematopoietic recovery and related cytokines in mice with transplant tumor after treated by chemotherapy. METHODS: The tumor cells were subcutaneously injected in the right forefoot to induce transplant solid tumor in mice. The mice were ig with PSP for 10 d and injected intraperitoneally with cyclophosphamide (CTX) for 3 d. On d 11, the count of peripheral blood cell, nucleated cell in bone marrow and CFU-S (Colony Forming Unit-Spleen) was observed; The content of DNA in bone marrow was inspected by UV-spectrophotometer; The cytokines in serums were inspected by double antibody sandwich ELISA. RESULTS: CTX could induce evident myelosuppression. But PSP could elevate the level of peripheral blood cell, increase nucleated cell and DNA in bone marrow, and promote CFU-S formation. In addition, PSP could also increase the content of IL-1, IL-3, GM-CSF, and TNF-? in serum. CONCLUSION: It is probably that PSP accelerates the hematopoietic recovery in mice with transplant tumor treated by chemotherapy by promoting endogenous cytokines secretion.

We learn the advanced philosophy and methods in dermatology residency standardized training program by introducing the residents matching system,periodical training programs and evaluation mechanisms in Taiwan.With the knowledge of current dermatology residency training system in mainland China,we give some recommendations to how to improve dermatology residency standardized training system in mainland China:standardizing and improving dermatology residence recruiting metrics,preparing effective training plan,and implementing better candidate evaluation processes as well as providing a strong faculty team.

AIM To observe the effect of polysaccharide from spirulina platensis(PSP) on lung injured by exposing ozone. METHODS The injury model of lung were induced by exposing ozone. The xanthine oxidase method and TBA were used to determine SOD and MDA in serum and lung respectively. The transmission electron microscope was used to observe the structural change of lung cell. RESULTS Compared with model mice, SOD in the groups of PSP administrated mice were increased and MDA were decreased significantly(P

OBJECTIVE: To investigate the preventive and therapeutic effects of Danshen Decoction on gastric ulcer in experimental animal models. METHODS: The model of water immersion restraint stress ulcer in mice, and the models of acetic acid impaired gastric ulcer and pyloric ligation gastric ulcer in rats were established. The total gastric acid, the activity of pepsin and the amount of gastric wall binding mucus were detected. RESULTS: Danshen Decoction reduced the acute gastric mucosal lesion. The effect of 5.0 or 10.0 g/kg group was obviously better than that of the normal saline control group (P<0.05 and P<0.01 respectively). Danshen Decoction accelerated the healing of ulcer induced by acetic acid significantly, and increased the content of gastric wall binding mucus (P<0.01); and it also inhibited the formation of gastric ulcer induced by pyloric ligation, while having no influence on the secretion of gastric acid and the activity of pepsin. CONCLUSION: Danshen Decoction has a significant anti-ulcerative effect, which may be related to improving the gastric mucosal defensive function.

Objective:To observe the living cells on the surface of intraocular lenses and to compare the cellular numbers and morphology of prestaining to that of poststaining.Methods:Twenty adult pigment rabbit eyes were given posterior chamber intraocular lens implantation.Lenses were extracted and put into 1640 cell cultural liquid immediately on the 1 st,3 rd,7 th,14 th and 28 th day respectively after operatrion.The samples were observed under inverted phase contrast microscope subsequently.Results:We found cells deposited on the surface of lenses from 1 st to 28 th day after operation.These cells contracted and lost three-demensional appearance while its number was less than that of prestaining obviously.Conclusion:A part of cells remove from the surface of lenses during staining;however,we could count the number but could not affirm cellular types which would make error in cellular classification and counting.The fact suggests that we should discover new accurate and reliable methods to avoid mistakes during experiment.

Objective To investigate the effect of nobiletin,a citrus polymethoxy flavonoid,on hepatocarcinoma Heps in mice and to explore its immunological mechanism.Methods Mice tumor models were made by transplantation of Heps hepatocarcinoma cell strain.The tumor mass was weighed to calculate the tumor-inhibitory rates.The whole cellular immunity was observed by delayed type hypersensitivity(DTH).MTT assay was used to detect the proliferation of T and B lymphocyte,and the activities of cytotoxic T lymphocytes(CTL) and natural killing cells(NKC) were measured by lactate dehydrogenase(LDH) releasing method.Results Nobiletin could markedly inhibit the growth of hepatocarcinoma Heps(solid tumor).The decreased DTH in hepatocaricinoma mice was enhanced significantly,and T and B lymphocyte proliferation were increased by nobiletin.Meanwhile,the killing activities of CTL and NKC were promoted obviously.Conclusion Improvement of the immune function may be a vital route of nobiletin in inhibiting the proliferation of hepatocarcinoma Heps in mice.