OBJECTIVE:To study the compatible stability of Ceftriaxone Sodium with Compound Sodium Chloride Injection or Sodium Lactate Ringer's Injection and study the compatible stability of the simulated in vivo peak concentration of ceftriaxone sodium with instant high concentration of calcium gluconate ion injection. METHODS: The concentration of Ceftriaxone Sodium in the mixture was determined by HPLC,meanwhile,the changes of the mixture in appearance,pH value,and the insoluble particles at room temperature were observed. RESULTS: At 0～2 hours,the mixture of Ceftriaxone Sodium and Compound Sodium Chloride Injection was clear in appearance;however,at 2 hours,it was white cloudy macroscopically,and its pH valued increased,ceftriaxone sodium content decreased and insoluble particles increased. The ceftriaxone sodium content reduced when mixed with sodium lactate ringer's injection. After mixing of simulated in vivo peak concentration of ceftriaxone sodium with instant high concentration of calcium gluconate ion injection,the appearance of the mixture was stable but the concentration of Ceftriaxone Sodium reduced slightly. CONCLUSION: Ceftriaxone Sodium can't be used in combination with Compound Sodium Chloride Injection or Sodium Lactate Ringer's Injection. Whether it is suitable to infuse calcium gluconate when ceftriaxone sodium (iv gtt) reached peak concentration remains to be confirmed in future study.

Objective To observe the effect of Danshen Yin on some gastric mucosal defensive factors in rats with acetic acid-induced gastric ulcer and to explore its anti-ulcer mechanisms.Methods Rat models with acetic acid-induced gastric ulcer were treated with Danshen Yin,ranitidine,and saline respectively for 14 days. After treatment,the ulcer index,serum nitric oxide(NO) level,plasma prostaglandin E2(PGE2)content and the number of micrangium in the granulation at the ulcer base were examined.Results The ulcer index in Dandhen Yin group and ranitidine group was significantly less than that in model group(P

AIM: To evaluate the effect of polysaccharide from spirulina platensis (PSP) on hematopoietic recovery and related cytokines in mice with transplant tumor after treated by chemotherapy. METHODS: The tumor cells were subcutaneously injected in the right forefoot to induce transplant solid tumor in mice. The mice were ig with PSP for 10 d and injected intraperitoneally with cyclophosphamide (CTX) for 3 d. On d 11, the count of peripheral blood cell, nucleated cell in bone marrow and CFU-S (Colony Forming Unit-Spleen) was observed; The content of DNA in bone marrow was inspected by UV-spectrophotometer; The cytokines in serums were inspected by double antibody sandwich ELISA. RESULTS: CTX could induce evident myelosuppression. But PSP could elevate the level of peripheral blood cell, increase nucleated cell and DNA in bone marrow, and promote CFU-S formation. In addition, PSP could also increase the content of IL-1, IL-3, GM-CSF, and TNF-? in serum. CONCLUSION: It is probably that PSP accelerates the hematopoietic recovery in mice with transplant tumor treated by chemotherapy by promoting endogenous cytokines secretion.

OBJECTIVE: To investigate the preventive and therapeutic effects of Danshen Decoction on gastric ulcer in experimental animal models. METHODS: The model of water immersion restraint stress ulcer in mice, and the models of acetic acid impaired gastric ulcer and pyloric ligation gastric ulcer in rats were established. The total gastric acid, the activity of pepsin and the amount of gastric wall binding mucus were detected. RESULTS: Danshen Decoction reduced the acute gastric mucosal lesion. The effect of 5.0 or 10.0 g/kg group was obviously better than that of the normal saline control group (P<0.05 and P<0.01 respectively). Danshen Decoction accelerated the healing of ulcer induced by acetic acid significantly, and increased the content of gastric wall binding mucus (P<0.01); and it also inhibited the formation of gastric ulcer induced by pyloric ligation, while having no influence on the secretion of gastric acid and the activity of pepsin. CONCLUSION: Danshen Decoction has a significant anti-ulcerative effect, which may be related to improving the gastric mucosal defensive function.

Objective To explore the puncture technic and method of the transabdominal L5/S1 lumbar diskectomy (TALD). Methods Two cases of cadaver were dissected and L5/S1 intervertebral disks were explored and punctured from different angles in order to probe the proper puncture angle for the needling. The radiographs of the lumbar and sacral vertabral samples of 30 cases were scrutinized and analysed to find the pancture imaging marks of the bones. When performing barium meal examinations, the lumbar and sacral spine of 200 cases were inspected to figure out the opacification of the rate imaging marks. 68 cases TALD were reviewed. Results The most pivotal process of the TALD is the needle puncture should be in parallel direction with lumbosacral interspace and then be pushed into the intervertebral disk. In order to make sure of the puncture point two sticking points should be fixed, on the abdomen and the another at the front edges of L5/S1 intervertebral disks. The five imaging makes of the lumbar, sacral, spine together with the neighboring L5/S1 intervertebral disks are of important help to find the front edges. Conclusion The correct puncture method is very important for performing TALD.

Objective To study the influence of anticipatory and post-event information processing on the memories and perception of the symptom in patients with social anxiety disorder (SAD). Methods A group of 32 SAD patients and a control group of 35 healthy indivisuals were included. Instruments including Self-Rating Depression Scale, Subjective Units Discomfort Scale( SUDS ), Rumination Questionnaire ,Open-ended Recall and Body Sensations Questionnaire were adopted in both groups. Results In the group SAD, no significant difference was identified between the experimental group( (49.68 ± 17.68), ( 19.00 ± 1.25), (0. 54 ±0. 17) ) and the experimental control group( ( 50.43 ± 20.72 ), ( 18.68 ± 1.25 ), ( 0.52 ± 0.17 ) ) when the ratings the memories of body sensations, rumination, and negative self-information score were compared (P＞ 0.05 ). There was a significant positive correlation between the level of rumination ,SUDS, the memories of negative self-information and the body sensations scores( r= 0.72; r= 0.94; r= 0.70, P＜ 0.01 ). The scores of rumination explained 64% of the variation in SUDS scores(β=0.82, P＜0. 01 ). Conclusion This study suggest that social anxiety is affected directly by rumination which can result in more memories of negative self-information and the body sensation. Symptoms are maintained by post-event information processing.

Objective To investigate the anti-HBV constituents from Alternanthera philoxeroides.Methods The constituents were isolated with silica gel and gel permeation chromatography,and purified by HPLC.Their structures were elucidated by spectroscopy.The antivirus effects of the isolated compounds were tested by ELISA method in vitro.Results Ten compounds were isolated and elucidated as followings:oleanolic acid(Ⅰ),oleanolic acid 3-O-?-D-glucuronopyranoside(Ⅱ),oleanolic acid 28-O-?-D-glucopyranoside(Ⅲ),chikusetsusaponin Ⅳ a methyl ester(Ⅳ),4,5-dihydroblumenol(Ⅴ),N-trans-feruloyl 3-methyldopamine(Ⅵ),N-trans-feruloyl tyramine(Ⅶ),3?-hydroxystigmast-5-en-7-one(Ⅷ),24-methylenecycloartanol(Ⅸ),and cycloeucalenol(Ⅹ).The values of inhibition percent of compounds Ⅰ-Ⅲ,Ⅴ-Ⅶ revealed a significant distinction compared to the control group.Compounds Ⅱ and Ⅴ showed significant inhibition against HepG2 cells transected with cloned hepatitis B virus DNA,their inhibitive ratios were 85.38% and 87.37% at 50 ?g/mL,respectively.Conclusion Compounds Ⅳ-Ⅶ are isolated from this plant for the first time and phenolic amides have been determined as the new structure type from the plants of Alternanthera Forsk.Compounds Ⅱ and Ⅴ from A.philoxeroides show the more significant anti-HBV activities.

Objective: To evaluate the antitumor effect of polysaccharide from spirulina platensis(PSP), and its synergic and attenuated effects on chemotherapeutic drugs in tumor-bearing mice. Methods: Mouse models of transplanting tumor were established. Antitumor effects of PSP and PSP combined with chemotherapeutic drugs were observed and the effect of PSP on leukopenia induced by chemotherapeutic drugs was also investigated. Results: PSP significantly inhibited S180 and H22, the inhibitory rate being 50 %-58 %and 43 %-51 %respectively. PSP evidently increased the antitumor action of cytoxan, PDD and 5-Fu, and elevated the number of white blood cells in leukopenia. Conclusion: PSP can obviously inhibit the tumor growth, also has synergic and attenuated effects on chemotherapeutic drugs.

AIM To observe the alteration of the functions and hypermicro-instructure in lung of experimental aging mice and the effect of cistanche desertica polysacchrides on the alteration. METHODS To use the models of inhaling ozone (O 3) for 4 week,and two doses of cistanche desertica polysacchrides (50,100 mg?kg -1 ?d -1 ) were ig administrated to small and large dose group mice per day respectively(for 6 week).Then the behavioral and bio-chemical examinations of lung and blood was carried out,and the ultrastructures were observe by the transmission electronic microscopic. RESULTS Compared with aging models,cistanche desertica polysacchrides could significantly prolong the period of non-oxygen ( P

Sixty-four patients with Graves' diseases were divided into methimazole group ( n =30 ) and propylthiouracil group( n =34 ).20 healthy volunteers were used as the control group.The levels of interleukin (IL) -2,IL-6,and TSH receptor antibody (TRAb) were determined by ELISA after serum samples were eollected before treatment and after treatment for 3 and 6 months.The results showed that the general data of two groups were not significantly different before treatment.IL-2 and IL-6 levels at 6 months after treatment were significantly different from the baseline (P＜ 0.05 ),IL-2 being gradually increased while IL-6 decreased with time.The level of IL-6 in methimazole group was lower than that in propylthiouracil group after treatment for 6 months ( P＜ 0.05 ).There was no significant difference in TRAb levels between two groups before treatment while differences became significant at 3 and 6 months of treatment ( all P＜ 0.01 ),being gradually decreased with time. IL-2/IL-6 ratio became significantly greater at 3 and 6 months of treatment compared with that before treatment in the same treatment group( P＜0.05 ) and the ratio in methimazole group was higher than that in propylthiouracil group by 6 months of treatment ( P＜0.05 ).IL-6level was positively correlated with FT3 and FT4 levels,and IL-2 level was negatively correlated with FT3,FT4,and TRAb levels in GD patients before treatment.The correlations of IL-2 and IL-6 with FT3 and FT4 disappeared by 3 and 6 months of treatment.IL-2 and IL-6 levels were related with TRAb level before and after treatment in methimazole group. These results suggest that the immunosuppressive effect of methimazole is more evident than that of propylthiouracil in patients with Graves' disease.