DNAzymes, screened through in vitro selection, were artificial nucleic acids with catalytic function . They could cleave specific substrates in the presence of cofactors with unique characteristics, such as high catalytic activity, high specificity for cofactors, excellent stability, and easy to synthesize and modify. The combination of DNAzymes with nanomaterials could retain the DNAzyme activity and realize the functional integration of recognition and signal transduction, promoting rapid development of biosensors. In the current paper, we mainly reviewed the recent progress in DNAzyme-nanomaterial based biosensors, and the nanomaterials included gold nanoparticles, graphene, quantum dots, magnetic nanomaterials, and so on.

Objective:To explore the effects of PRECEDE model combined with diet guidance in continuing nursing for patients with type 2 diabetes mellitus (T2DM) .Methods:The convenience sampling was used to select 108 T2DM patients from the Affiliated Hospital of Jining Medical University from August 2017 to April 2019, and they were divided into control group and observation group with the method of random number table, with 54 cases in each group. Control group carried out the routine follow-up nursing. On this basis, observation group implemented the PRECEDE model combined with diet guidance. We compared the glucose metabolism related indicators [fasting blood glucose, 2-hour postprandial blood glucose and glycosylated hemoglobin (HbA1c) ], and self-management ability score of patients between two groups at discharge and six months after discharge, and health knowledge mastery as well as chronic complications of those six months after discharge.Results:At discharge, there was no statistical difference in blood glucose indicators and score of self-management ability of patients between two groups ( P>0.05) . Six months after discharge, fasting blood glucose, 2-hour postprandial blood glucose and HbA1c of patients between two groups were lower than those before discharge, and those of observation group were lower than those of control group, the differences were statistically significant ( P<0.01) . Six months after discharge, there was no statistically significant difference in the score of self-management ability of patients in control group compared with that before discharge ( P>0.05) ; scores of self-management ability of patients in observation group six months after discharge were higher than those before discharge and those of control group with statistical differences ( P<0.01) . The rate of good scores on the Health Knowledge Questionnaire of patients in observation group six months after discharge was higher than that in control group with a statistical difference ( P<0.05) . Followed up for six months, the total incidence of complication of observation group was lower than that of control group also with a statistical difference ( P<0.01) . Conclusions:The application of PRECEDE model combined with diet guidance in the follow-up of T2DM patients can improve patients' health knowledge and self-management ability after discharge, help blood glucose control and reduce complications.

Objective:To understand the current situation of occupational stress and job burnout of male clinical nurses, and to explore the influencing factors of job burnout of male nurses.Methods:An electronic questionnaire collection system was used to select male nurses from 4 general hospitals in Jining City in October 2020 by cluster sampling. The investigation was conducted with the General Information Questionnaire, Job Stress Scale, and Maslach Burnout Inventory. A total of 540 questionnaires were distributed and 514 valid questionnaires were returned with a valid response rate of 95.19%.Results:Among 514 male nurses, the total score of the Job Stress Scale was 157.50 (144.00, 171.00) , and the total score of the Maslach Burnout Inventory was (29.07±17.36) . The results of hierarchical regression analysis showed that emotional exhaustion in the Maslach Burnout Inventory could explain 23.8% of the total variation of male nurses' occupational stress ( P<0.05) . Conclusions:The occupational stress of male nurses is correlated with job burnout. Nursing managers should pay attention to the occupational stress of male nurses in their career development, and take effective measures from the management level to assist male nurses in coping with setbacks and difficulties, and reduce the level of occupational stress.

Objective To study the effects of Jaridonin, a novel diterpenoid from isodon rubescens, on the cell cycle of human gastric cancer cells and its molecular mechanism of action. Methods Flow cytometry was used to analyze the cell cycle distribution and expression of ataxia telangiectasia mutated kinase ( ATM) after Jaridonin treatment. Western blot was performed to detect the expression of cell cycle?related proteins. Results The results of flow cytometry showed that the percentages of MGC?803 cells in G2/M phase at 6 hours after 0, 10, 20 μmol/L Jaridonin?treatment were (10.8±2.2)%, (18.2±2.5)%, (27.3±3.2)%, respectively;those at 12 hours after Jaridonin?treatment were (12.0±1.5)%, (24.1±2.0)%and (39.7±5.2)%, respectively, indicating a G2/M phase arrest of MGC?803 cells was resulted in a time?and dose?dependent manner. The expressions of ATM, Chk1, Chk2, phosphorylated Cdc2 and CDK2 were up?regulated in the MGC?803 cells after Jaridonin treatment, while the levels of Cdc2 and CDK2 were decreased. KU?55933, an inhibitor of ATM, reversed the expression of relevant proteins and G2/M phase arrest induced by Jaridonin. Conclusions Jaridonin can significantly induce G2/M arrest in gastric cancer MGC?803 cells. Its mechanism may be related to the activation of ATM and Chk1/2, and inactivation of Cdc2 and CDK2 phosphorylation.

Objective To study the effects of Jaridonin, a novel diterpenoid from isodon rubescens, on the cell cycle of human gastric cancer cells and its molecular mechanism of action. Methods Flow cytometry was used to analyze the cell cycle distribution and expression of ataxia telangiectasia mutated kinase ( ATM) after Jaridonin treatment. Western blot was performed to detect the expression of cell cycle?related proteins. Results The results of flow cytometry showed that the percentages of MGC?803 cells in G2/M phase at 6 hours after 0, 10, 20 μmol/L Jaridonin?treatment were (10.8±2.2)%, (18.2±2.5)%, (27.3±3.2)%, respectively;those at 12 hours after Jaridonin?treatment were (12.0±1.5)%, (24.1±2.0)%and (39.7±5.2)%, respectively, indicating a G2/M phase arrest of MGC?803 cells was resulted in a time?and dose?dependent manner. The expressions of ATM, Chk1, Chk2, phosphorylated Cdc2 and CDK2 were up?regulated in the MGC?803 cells after Jaridonin treatment, while the levels of Cdc2 and CDK2 were decreased. KU?55933, an inhibitor of ATM, reversed the expression of relevant proteins and G2/M phase arrest induced by Jaridonin. Conclusions Jaridonin can significantly induce G2/M arrest in gastric cancer MGC?803 cells. Its mechanism may be related to the activation of ATM and Chk1/2, and inactivation of Cdc2 and CDK2 phosphorylation.

Objective:To explore effects of the total number of night shifts on BMI of medical workers in recent 5 years.Methods:This study was a cross-sectional study. Using the convenient sampling method, data of medical workers in Affiliated Hospital of Jining Medical University were collected from January 2016 to December 2020. The medical examination data of medical workers were obtained from the electronic information system of medical examination center, and the number of night shift and basic information of medical workers are extracted from the human resource management department. The covariates in this study included categorical variables and continuous variables. Categorical variables included gender, job title and job type. Continuous variables included age, working years, systolic blood pressure, diastolic blood pressure, white blood cells, red blood cells, hemoglobin, average hemoglobin volume, platelets, ALT, total bilirubin, total protein, albumin, urea, creatinine, uric acid, triacylglycerol, Total cholesterol, HDL-C, LDL-C and glucose. The relationship between the number of night shift and BMI was processed by generalized addition model and smoothing curve fitting.Results:Finally, a total of 908 medical workers were selected for data analysis. The average number of night shifts for 908 medical workers in 5 years was (339.0±30.8) , and the average BMI was (22.88±2.08) kg/m 2. After adjusting for confounding factors, the number of night shift showed a non-linear relationship with BMI, and the number of inflection points was 634. On the left of the inflection point, there was no significant relationship between the number of night shifts and BMI ( P=0.829) . The relationship between the two on the right side of the inflection point was significant, the effect size and 95% confidence interval were 0.02 and 0.01-0.03, respectively. Conclusions:BMI value of medical workers increases significantly with the increase of night shift number when the number of night shift is more than 634 in recent 5 years. Hospital managers can calculate and plan the number of night shifts per year to reduce the effect of night shifts on the health of medical workers.

OBJECTIVETo explore the associations between the genetic variations in the SDC2 gene and overall survival and risk of radiation esophagitis in patients with esophageal squamous cell carcinoma (ESCC).

METHODSEleven functional haplotype-tagging single nucleotide polymorphisms (htSNPs) of SDC2 were genotyped in 296 ESCC patients who received radiotherapy alone, and had different response and esophagitis. The associations between genotypes and risk of esophagitis were measured by odds ratios (ORs) and 95% confidence intervals (CIs), adjusted for sex, age, tumor location, staging, radiotherapy mode and total radiation dose. The hazard ratios (HRs) were estimated using Cox proportional hazards regression model.

RESULTSThe median survival time (MST) of these patients was 14 months. Of them, 260 (87.8%) had died until the last date of follow-up of 30 June, 2014. Clinical stage (stage IV vs. stage II) and total radiation dose (≥ 60 Gy vs. < 60 Gy) influence the overall survival time of the patient significantly. Cox proportional hazards regression model analysis showed that the subjects with rs61599409 T allele had an decreased hazard ratio as compared with those with C allele (adjusted HR = 0.82, 95% CI, 0.66-1.02), but the difference was not statistically significant (P = 0.071). The rest 10 htSNPs were not associated with the overall survival of ESCC patients treated with radiotherapy. Among this set of patients, 160 (54.1%) suffered from radiation esophagitis. We found that rs17788084 A > T SNP in the 3'-untranslational region of SDC2 was associated with esophagitis risk, with the OR being 0.48 (95% CI = 0.28-0.85, P = 0.011) for the TA or TT genotype compared with the AA genotype.

CONCLUSIONSThese results suggest that rs17788084 genetic variation in SDC2 is associated with risk of radiation esophagitis and might serve as a potential biomarker for personalized radiotherapy of ESCC.

Alleles ; Carcinoma, Squamous Cell ; mortality ; pathology ; radiotherapy ; Esophageal Neoplasms ; mortality ; pathology ; radiotherapy ; Esophagitis ; genetics ; Genetic Variation ; Genotype ; Haplotypes ; Humans ; Odds Ratio ; Polymorphism, Single Nucleotide ; Proportional Hazards Models ; Radiation Injuries ; genetics ; Radiotherapy Dosage ; Risk ; Survival Analysis ; Syndecan-2 ; genetics ; Time Factors

model. Results The median survival time (MST) of these patients was 14 months. Of them, 260 (87.8%) had died until the last date of follow?up of 30 June, 2014. Clinical stage ( stage Ⅳ vs. stage Ⅱ) and total radiation dose (≥ 60 Gy vs. <60 Gy) influence the overall survival time of the patient significantly. Cox proportional hazards regression model analysis showed that the subjects with rs61599409 T allele had an decreased hazard ratio as compared with those with C allele ( adjusted HR=0.82, 95% CI, 0.66?1.02) , but the difference was not statistically significant ( P=0.071) . The rest 10 htSNPs were not associated with the overall survival of ESCC patients treated with radiotherapy. Among this set of patients, 160 ( 54. 1%) suffered from radiation esophagitis. We found that rs17788084 A > T SNP in the 3′?untranslational region of SDC2 was associated with esophagitis risk, with the OR being 0.48 (95% CI=0.28?0.85, P=0.011) for the TA or TT genotype compared with the AA genotype. Conclusions These results suggest that rs17788084 genetic variation in SDC2 is associated with risk of radiation esophagitis and might serve as a potential biomarker for personalized radiotherapy of ESCC.

model. Results The median survival time (MST) of these patients was 14 months. Of them, 260 (87.8%) had died until the last date of follow?up of 30 June, 2014. Clinical stage ( stage Ⅳ vs. stage Ⅱ) and total radiation dose (≥ 60 Gy vs. <60 Gy) influence the overall survival time of the patient significantly. Cox proportional hazards regression model analysis showed that the subjects with rs61599409 T allele had an decreased hazard ratio as compared with those with C allele ( adjusted HR=0.82, 95% CI, 0.66?1.02) , but the difference was not statistically significant ( P=0.071) . The rest 10 htSNPs were not associated with the overall survival of ESCC patients treated with radiotherapy. Among this set of patients, 160 ( 54. 1%) suffered from radiation esophagitis. We found that rs17788084 A > T SNP in the 3′?untranslational region of SDC2 was associated with esophagitis risk, with the OR being 0.48 (95% CI=0.28?0.85, P=0.011) for the TA or TT genotype compared with the AA genotype. Conclusions These results suggest that rs17788084 genetic variation in SDC2 is associated with risk of radiation esophagitis and might serve as a potential biomarker for personalized radiotherapy of ESCC.