OBJECTIVE:To establish the microemulsion TLC for the separation and identification of various effective compositions in Changji'an prescription.METHODS: The compositions in Changji'an prescription were identified on polyamide film(fixed phase) with five distribution ratios of sodium lauryl sulphate-n-butanol-skellysolve C-H2O microemulsions as developing agents.The effects of factors such as the water content of the microemulsion and the temperature on the separation efficacy were studied.RESULTS: With microemulsion(75% water content) as developing agent,Rhizoma Coptidis in Changji'an prescription was detected;with microemulsion(75% water content)-formic acid(9.0∶1.0) as developing agent,Corydalis yanhusuo,Saposhnikovia divaricata,chlorogenic acid and Radix et Rhizoma Glycyrrhizae in Changji'an prescription were detected.CONCLUSION: Microemulsion TLC is simple,environmental-friendly,sensitive with ideal separation efficacy,and it can be used for the separation and identification of various compositions in Chinese patent medicines.

Objective To explore the sensitive blood plasma molecular markers in diabetic nephropathy (DN) .Methods Two-di-mensional fluorescence difference gel electrophoresis (2D-DIGE) was used to analyze early DN patients plasma (n=8) and normal plasma ,proteins that showed differential expression of a 1 .5 fold change were analyzed by MALDI-TOF/TOF mass spectrometry . Results 2D-DIGE maps of plasma proteins in patients with DN and normal plasma were established successfully .We validated 13 differentially expressed proteins detected by 2D-DIGE ,including complement component C3、complement component C4、apolipopro-tein E ,etc .Conclusion Proteomic analysis can objectively revealed the differences of protein expression between the two kinds of blood plasma .The 13 proteins might be the potential plasma molecular markers for the clinical diagnosis of diabetic nephropathy .

Objective To screen the sensitive plasma molecular markers in diabetic nephropathy ( DN) patients with kidney yang deficiency. Methods Two-dimensional fluorescence difference gel electrophoresis (2-D DIGE) was used to analyze the plasma which was sampled from 4 early DN patients with kidney yang deficiency and 4 healthy adult volunteers. Proteins that showed differential expression with an over 1.5-fold change were analyzed by MALDI-TOF/TOF mass spectrometry. Results Two-dimensional electrophoresis (2-DE) maps of plasma proteins in DN patients with kidney yang deficiency and healthy adults were established successfully. We validated 9 differentially expressed proteins, including complements C3 and C4, apolipoprotein E and ubiquitination factor. Conclusion Proteomic analysis by 2-D DIGE can objectively reveal the differences of plasma protein expression in DN patents with kidney yang deficiency and healthy adult volunteers. The obtained 9 proteins have potential to provide plasma molecular markers for the early clinical diagnosis and for the research of traditional Chinese medical patterns of DN.

0.05). Conclusion The extract technology is basically reasonable,and need further improvement.

Objective To investigate the role of 8 iso PGF2a in patients with chronic obstructive pulmonary disease(COPD).Methods Level of serum 8 iso PGF2a and lung function were studied in 38 patients with acute exacerbation of COPD(AECOPD),23 patients with stable COPD and 18 health subjects.Results The serum 8 iso PGF2a level was significantly higher in patients with AECOPD[(6.84?1.70)?g/L]as compared to those in the controls[(4.64?1.24)?g/L]and in patients with stable COPD[(5.82?1.72)?g/L](P

[Objective] To develop a method for the determination of thiamazole content in Yingqi Ling Tablets. [Methods] High performance liquid chromatography (HPLC) was used. The chromatographic conditions were: C18 Gravity Column (4.6mm ? 250mm), methanol - water (10:90 ) as mobile phase, flow rate being 1.0mL/min and the detection wavelength at 258nm. [Results] The calibration curve was linear in the range of 0.16 - 0.64?g. The average recovery was 101.01% (relative standard deviation sr = 2.21%). Relative standard deviation of precision test was 1.04% and the content of the sample was 0.4841 mg per tablet (sR = 0.78%). [ Conclusion] HPLC is effective for the determination of thiamazole content in Yingqi Ling Tablets.

Objective To investigate the feasibility of the gene therapy on angiogenesis after myocardial infarction in rats.Methods Thirty-six male SD rats,after the ligation of left anterior descending coronary artery,were divided into 2 groups as experimental and control one.Expressions of VEGF were measured by RT-PCR and Immunohistochemistry(IHC).Angiogenesis and capillary density were evaluated by HE stain,and qualitative and quantitative analysis were carried out.The adverse effects were tested after injection of pVEGF165-PLGA nanoparticle.Results Compared with control groups,ischemic myocardial cells persistently and stably expressed VEGF in experimental group;Vascular endothelial cells actively proliferated,and the effect of angiogenesis was significant;48 hours later,nanoparticles were observed in myocardial cells.Conclusion Injection of with pVEGF165-PLGA nanoparticle,it can stimulate effective host-derived angiogenesis,which results in the prevention of impaired cardiac muscle after myocardial infarction.It may be an effect way to treat MI.

No abstract available.
Comorbidity* ; Depression* ; Irritable Bowel Syndrome* ; Neuronal Plasticity*

Objective To determine the prognosis and risk factors of neonatal cerebral infarction.Methods From January 2002 to December 2010,44 newborn infants were diagnosed with cerebral infarction by imaging examinations at Peking University First Hospital.The neurodevelopmental outcomes of these newborn infants were followed up and evaluated by clinical manifestations,Gesell development scale,cranial imaging,electroencephalogram and auditory evoked potential.Factors related to prognosis were analyzed with single and multi-factor Logistic regression analysis.Results Thirty-eight (86％) cases were followed up,and of these cases,five children died and the results of three were inconclusive due to small age (less than 6 months old).Among the remaining 30 children,neurodevelopmental outcome was normal in 15 cases and abnormal in the remaining 15 cases,thus,the incidence of sequelae was 50％ (15/30) and the mortality rate was 13％ (5/38).Of the 15 abnormal cases,all had cerebral palsy and movement retardation,eight cases had cognitive impairment,eight cases had epilepsy and five had visual impairment.The incidence of large cerebral infarction (more than one lobe) was 14/15,worse cranial imaging outcome (one month after treatment,cerebral infarction lesion still present or had expanded)was 13/15,and severe complications was 8/15 in the newborns with sequelae,which were higher than in those without sequelae (4/15,5/15 and 1/15,respectively) (x2=13.889,8.889 and 7.778,all P＜0.05).Logistic regression analysis showed that large cerebral infarction was a risk factor for sequelae (OR=38.500,95％C1:3.749-395.407,P=0.002),however,worse cranial imaging outcome (OR=8.563,95％CI:0.909-80.683,P=0.061) and severe complications (OR=18.024,95％CI:0.516-630.163,P=0.111) were not risk factors for sequelae.Cerebral infarction with middle cerebral artery injury had a high risk of movement retardation (OR=6.000,95％CI:1.172-3.725,P=0.025),and those with a large cerebral infarction were more likely to have epilepsy (x2=7.273,P=0.010).The incidence of large cerebral infarction in the newborn infants with cognitive impairment was 8/8,which was much higher than in those without cognitive impairment (46％,10/22),thus,infarct area may be related to cognitive ability (x2=7.273,P=0.010).Conclusions Neonatal cerebral infarction might result in many types of sequelae,with motor impairment being the most common form.A large cerebral infarction is more likely to result in abnormal neurodevelopmental outcome.

Objective To investigate the correlation between hypoglycemia and brain injury of newborns.Methods Medical records and follow-up data of 110 newborns with hypoglycemia (blood glucose level≤2.2 mmol/L) who admitted into neonatal department of Peking University First Hospital from December 2006 to December 2009 were studied.All patients were divided into 3 groups:no brain injury group,mild and severe brain injury group according to their clinical manifestation,cerebral radiological characteristics and cerebral functional tests.By using receiver operating characteristic (ROC) curve and x2 test,the potential optimal blood glucose level and duration of hypoglycemia for predicting brain injury were confirmed.Multivariate Logistic regression was taken to determine independent predictors for brain injury.The analyzed factors included gender,preterm/small for gestational age,hyperbillirubinemia,fetal distress,asphyxia,infection,seizures and maternal hypertensive disorder complicating pregnancy and hyperglycemia.Results Among the 110 hypoglycemia newborns,33 (30.0％) infants suffered from brain injury,of which 23 were mild and 10 were severe.Blood glucose ≤1.7 mmol/L had high specificity (73％) and sensitivity (60％)for predicting brain injury.When blood glucose≤ 1.7 mmol/L,the incidence of brain injury and severe brain injury was 43.6％ (24/55) and 18.2％ (10/55),which was higher than those [16.4％(9/55) and 0.0％ (0/55)] of patients whose glucose level ＞1.7 mmol/L(x2 =9.74 and 11.00,P＜0.01 respectively).Blood glucose ≤ 1.2 mmol/L had high specificity (100％) and sensitivity (81％) for predicting severe brain injury.When blood glucose ≤1.2 mmol/L,the incidence of severe brain injury was higher than that of the patients whose glucose level was higher than 1.2 mmol/L [34.5％ (10/29) vs 0.0％ (0/81),x2 =30.72,P＜0.01].Duration of hypoglycemia ≥12 h had specificity (100％) and sensitivity (36 ％) for predicting brain injury.When duration of hypoglycemia ＜12 h,the incidence of brain injury was lower than that of the patients whose duration of hypoglycemia≥12 h [21.4％ (21/98) vs 6/6,x2 =27.69,P＜0.01].Multivariate Logistic regression showed that fetal distress (OR=4.69,95％CI:1.47-14.97,P=0.009),glucose level≤1.2 mmol/L (OR =5.16,95％CI:1.56-17.03,P=0.007),duration of hypoglycemia≥12 h (OR=8 885 220 297.12,95％CI:0.00-∞,P =0.000) and maternal hyperglycemia (OR =3.34,95％CI:1.01-11.02,P=0.048) were independent risk factors for neonatal brain injury.Conclusions Low blood glucose level and prolonged hypoglycemia might induce injury of neurol system.Fetal distress and maternal hyperglycemia might increase the incidence of brain injury in newborns with hypoglycemia.