Objective To conclude nursing experience of second anastomotic leakage after radical gastric cancer surgery. Methods The present study compared the differences of clinical symptoms between these two anastomotic leakages, and found a way to detect latent anastomotic leakage with an unobvious abdominal sign accurately and opportunely. The study also intervened in the patient′s observation, psychological condition, peritoneal irrigation and nutritional support after surgery. Results The patients were discharged from the hospital after cured with well controlled condition. Conclusions In order to make an early detection and treatment of the second anastomotic leakage, nurses should not only observe the abdominal sign, but also observe the systematic poisoning and concomitant symptoms.

AIM To investigate the roles of Cl- channels in Ca2+ influx induced by activaion of al- adrenoceptor subtypes in transfected-CHO cells. METHODS The effects of drugs on ?1A、?1B and ?1D- AR-induced Ca2+ influx were investigated with Fura2 fluorescence technique. RESULTS The ?1A-AR- induced Ca2+ influx was inhibited by furosemide(2 .5 ~ 10 M?mol?L- 1 )and SK&F96365(5- 15 ?mol?L- 1 ) in a concentration- dependent manner respectively; The ?1B-AR-induced Ca2+ influx could also be inhibit inhibited by NFA(2. 5 ~ 10 ?mol? L-1 ), whereas the alD AR-induced Ca2+ influx was only suppressed by NFA. In ?1B-CHO cells, Adr-triggered Ca2+ influx could be further inhibited by NFA or furosemide after the maximal inhibition by SK&F96365;SK&F96365 could further inhibit Ca2+ influx which had been inhibited by NFA or furosemide. In ?1A-CHO cells, Adr-triggered Ca2+ influx could be further inhibited by SK&F96365 after had been inhibited by furosemide; furosemide could not further inhibite Ca2+ influx which had been inhibited by S&F96365. CONCLUSION There are different characteristics of CI- channels related to ?1A、 ?1B and ?1D-AR-induced Ca2+ influx.

Objective:To observe the efficacys and side effects of gefitinib(Iressa)and docetaxel(aisu)as the second-line treatment for advanced non-small cell lung cancer(NSCLC)patients.Methods:A total of 98 patients with advanced non-small cell lung cancer who had failed to previous fimt-line chemotherapy were randomly divided into two groups:gefitinib group and docetaxel group.In the gefitinib group,50 patients took Iressa 250 mg once daily until disease progression or intolerable toxicity occurred.In the docetaxel group,58 patients were treated with aisu 75mg/m~2 i.v.in 60 minutes in day 1.The regimen was repeated every 21 days for at least 2 consecutive cycles.They were assessed on the basis of RECIST evaluation standard of therapeutic effect for solid tumor.Results:In the gefitinib group,the response rate was 22.4%(11/49),the clinical benefit rate was 55.1%(27/49),median survival time was 7.1 months and 1-year survival rate was 35.9%.In the docetaxel group,the response rate,clinical benefit rate,median survival time and 1-year survival rate were 18.8%(9/48),50.0%(24/48),6.9months and 31.5%,respectively(P>0.05).The incidences of Ⅰ～Ⅳ degree and Ⅲ～Ⅳ degree rash were 51.0%and 10.2%in the gefitinib group,significantly lower than those in the docetaxel group (P=0.0000 and P=0.0296).The incidence of Ⅰ～Ⅳ degree diarrhea was significantly higher in the gefitinib group than that in the docetaxel group(P<0.01).The incidence of leukopenia was significantly higher in the docetaxel group than that in the gefitinib group(P=0.0000).The incidences of thrombocytopenia and anemia of Ⅰ～Ⅳ degree were also higher in the docetaxel group (P=0.0000 and P=0.0266).The improvement rate of quality of life was higher in the gefitinib group (P<0.05).Conclusion:Gefitinib has a similar anti-tumor effect as docetaxel on advanced NSCLC patients who have failed to previous fimt-line chemotherapy.Gefitnib can achieve higher improvement rate of quality of life in advanced NSCLC patients,with a lower incidence of toxicity.

Objective Testing the CRP and PLT for patients with acute myocardium infarction(AMI) .Analysising the relation‐ship between AMI and CPR or PLT .Methods We chose 60 patients with AMI who treat in our hospital during 2012/11 and 2014/10 as an observation group .As the same time ,we also chose 60 healthy person as a comparison group .Testing the CRP and PLT of the two groups with the same method ,and then we compare and analysis the results .Results The observation group's concentration of CRP is (22 .13 ± 4 .71)mg/L ,level of PLT is (241 ± 33)× 109/L .The comparison group's concentration of CRP is(2 .74 ± 0 .49) mg/L ,level of PLT is(162 ± 26) × 109/L .The result of the observation group is obvious higher than the comparison group ,and the difference is significance(P<0 .05) .The observation group's positive rate of CRP is 73 .33% ,increase of PLT is 38 .33% .The comparison group's positive rate of CRP is 3 .33% ,increase of PLT is 5 .00% .The result of the observation group is obvious higher than the comparison group ,and the difference is significance(P<0 .05) .Conclusion To the clinical diagnosis of AMI ,testing the CRP concentration and the PLT level is useful to understand the patient's host defenses and inflammation condition .It has clinical value to AM I's prevent ,diagnosis and prognosis .

Objective Study on the diagnosis value of expression of serum macrophage inhibitory factor 1 (MIC-1) combined with alpha-fetoprotein isoforms 3 (AFP-L3) in primary liver cancer detected by enzyme linked immunosorbent assay (ELISA).Methods MIC-1 and AFP-L3 concentrations were detected by ELISA from selected 116 patients of primary liver cancer.Results were compared both in combined and sigle detection.Results In primary liver cancer group AFP-L3 concentration [(127.12±51.43) ngmml] was significantly higher than that in normal control group [(27.11±7.26) ng/ml,P ＜ 0.001].With AFP-L3 ＞ 38.0 ng/ml as the critical value,the sensitivity was 85.34 ％ (99/116),specificity was 88.33 ％ (53/60) and the diagnostic accuracy was 86.36 ％ (152/176).In primary liver cancer group MIC-1 concentration [(3140.43±1138.23) pg/ml]was significantly higher than that in normal the control group [(701.88±302.34) pg/ml,P ＜ 0.001],the sensitivity was 91.38 ％ (106/116),specificity was 85.00 ％ (51/60),the diagnostic accuracy was 89.20 ％(157/176).The two combined detection sensitivity was 83.62 ％ (97/116),specificity was 91.67 ％ (55/60),diagnostic accuracy was 86.36 ％ (152/176).Conclusion MIC-1 combined with AFP-L3 concentration detection can improve the specificity of the diagnosis of primary liver cancer,which has certain clinical value.

Objective:To summarize the complications' prevention and nursing experience for patients after autologous transplantation of the microvascular submandibular gland for severe keratoconjunctivis sicca.Methods:Retrospective analysis of 141 patients(151 sides) after autologous microvascular submandibular gland transfer operation was undertaken to record the timing and incidence of major complications and to summarize the nursing experience.Results: The main complications after the transplantation were: vascular crisis,catheter obstruction,salivary fistula and temporary paralysis of hypoglossal nerve.Related nursing measures in response to these complications were discussed.Conclusion:Targeted nur-sing interventions for patients with autologous transplantation the submandibular gland have a positive effect on the prevention of the surgical complications.

AIM: To investigate the relationship between chloride channels and the Ca 2+influx induced by adrendine(Adr). METHODS: The effects of drugs on Adr-induced Ca 2+influx were investigated with Fura-2 fluorescence technique. RESULTS: Adr-induced Ca 2+influx was inhibited by nifedipine,SK&F96365,NFA and furosemide in a concentration manner respectively; Ca 2+influx could be further inhibited by NFA or furosemide after the maximal inhibition by SK&F96365;SK&F96365 also could further inhibit the Ca 2+influx which had been inhibited by NFA or furosemide. Genistein and vanadate could reduce or increase the Ca 2+influx respectively. CONCLUSION: Ca 2+influx induced by Adr is related to VDC and ROC, and chloride channels involves in the processes.The levels of tyrosine phosphoralation affect the Ca 2+influx.

Objective To study the diagnostic value of the golgi protein 73 (GP73) combined with alpha‐fetoprotein (AFP) ,α‐L‐fucosidase (AFU) andα‐antitrypsin(α‐AT) detection in primary hepatic carcinoma(PHC) .Methods 100 patients with PHC ,100 patients with hepatitis and cirrhosis ,and 100 healthy people hospitalized in the hospital from October 2011 to May 2013 were en‐rolled in the study .Serum AFP levels were detected by using Beckman DxI800 chemiluminescent analyzer ,serum AFU ,α‐AT levels were detected by using Beckman DxC800 automatic biochemical analyzer ,and GP73 levels were measured by using enzyme‐linked immunosorbent GP73 quantitative detection kits .Results In patients with PHC and patients with hepatitis and cirrhosis ,serum GP73 ,AFP ,AFU ,α‐AT levels were significantly higher than healthy people(P<0 .05) .In patients with PHC ,GP73 ,AFP ,AFU ,α‐AT levels were significantly higher than those in patients with hepatitis and cirrhosis(P<0 .05) .The sensitivities of single serum GP73 ,AFP ,AFU ,α‐AT detection were 75% ,72% ,23% ,67% respectively and specificity were 95% ,95% ,100% ,97% respective‐ly .The sensitivity and specificity of the 4 tumor markers′combined detection were 99% and 100% .The sensitivity of combined de‐tection was significantly different from single detection(P<0 .05) .Conclusion GP73 ,AFP ,AFU ,α‐AT may improve the diagnostic efficiency of primary liver cancer .

Objective To explore the relationship between health-promoting lifestyle and work engagement in nurses of traditional Chinese medicine hospitals. Methods Totally 527 nurses from 3A level hospitals of the first, second, third affiliated to Zhejiang University of Traditional Chinese Medicine were investigated by the Chinese revision of Health-Promoting Lifestyle Scale, Chinese version of Utrecht Work Engagement Scale. Results M ultiple regression analysis showed that nurse health-promoting lifestyle had a significant correlation with work engagement, F = 8.987, P < 0.01. Among them, the self-realization dimension to the nurse work engagement most affected, t = 3.287, P < 0.01. Conclusions Improving nurses′health-promoting lifestyle can raise the level of work engagement and stabilize nursing staff and improve quality of care in traditional Chinese medicine hospitals.

OBJECTIVE To study and improve the washing methods for the metal piping medical instruments. METHODS Some contaminated metal uteroventral scraping and drawing instruments were randomly divided into group A(by the ultrasonic vibration with proteinase washing method)and group B(by the traditional manual washing method). The effects of two washing methods were studied. RESULTS The qualified rate of experimental samples in the group A was higher than that in the group B(P