Objective To explore the effect of comprehensive nursing intervention on the rehabilitation of patients with schizophrenia,and to provide reference for clinical nursing.Methods Sixty-seven long-term hospitalized patients with schizophrenia received 6 months comprehensive nursing intervention.The instruments of the nurse observation scale for inpatient evaluation(NOSIE),insight and treatment attitude questionnaire (ITAQ),scale of social function in psychosis inpatients (SSPI) were administered both at the start and the end of intervention.Result After 6 months of treatment,the scores on mental disorder,recovery of social ability and treatment compliance and recovery or selfconcicousness increased as compared with pre-intervention (all P＜0.001).Conclusion The comprehensive nursing intervention can improve the mental symptoms and social function,alleviate their depression and promote mental rehabilitation of the patients with schizophrenia.

Objective To investigate the relationship between platelet maximum aggregation rate(MAR),platelet thrombocyt-ocrit(PCT),platelet count(PLT),platelet distribution width(PDW)and mean platelet volume(MPV)with the course of acute cere-bral infarction(ACI)to provide the basis for its clinical early diagnosis and treatment.Methods 107 patients with ACI in our hospi-tal were selected and divided into the great infarction group(infarction size >10 cm3 ),middle infarction group(infarction size 4-10 cm3 )and small infarction group(infarction size<4 cm3 )according to the infarction lesion size by head CT or MRI and the infarction volume calculated by the Pullicino formula(length×width×layer number/2),40 healthy individuals were selected as the healthy control group.MAR,PLT,PDW,MPV and PCT were detected before and after the induction by PLR-06.Results (1 )Compared with the control group,PLT,PCT,PDW and MPV before the induction by PLR-06 in the great infarction group were obviously in-creased(P <0.01);PLT,PCT,PDW and MPV in the middle and small infarction groups were increased(P <0.05).(2)Compared with the control group,MAR after the induction by PLR-06 in each infarction group was increased(P <0.05);PLT had no statisti-cal difference among the groups(P >0.05 );PCT,PDW and MPV in the great and middle infarction groups were increased(P <0.05);PCT,PDW and MPV in the small infarction group had no statistical differences(P >0.05).Conclusion The change of the platelet aggregation rate,number and volume is closely related with the occurrence and development of ACI,monitoring their change has important clinical significance to prevention and treat ACI.

Objective:To explore the mechanism of 14-3-3σgene in regulating inflammatory response of human pulmonary epithelial cells induced by endotoxin/lipopolysaccharide (LPS).Methods:(1) Cells of human normal pulmonary epithelial cell line BEAS-2B cultured in logarithmic growth period were collected and divided into control group and PCMV6-14-3-3σgroup using the random number table, with 3 wells in each group. Cells in control group were transfected with empty plasmid, and cells in PCMV6-14-3-3σgroup were transfected with PCMV6-14-3-3σplasmid. The protein expression of 14-3-3σin cell was detected by Western blotting at 48 hours after transfection. (2) Cells of human normal pulmonary epithelial cell line BEAS-2B cultured in logarithmic growth period were collected and divided into control group, PCMV6-14-3-3σgroup, PCMV6-14-3-3σ+ LPS group, and LPS group using the random number table, with 3 wells in each group. Cells in control group were transfected with empty plasmid for 42 hours. Cells in PCMV6-14-3-3σgroup were transfected with PCMV6-14-3-3σplasmid for 42 hours. Cells in PCMV6-14-3-3σ+ LPS group were stimulated with 1 μg/mL LPS (the same final mass concentration below) for 6 hours after being transfected with PCMV6-14-3-3σplasmid for 42 hours. Cells in LPS group were stimulated by LPS for 6 hours. The protein expressions of Bax and B-cell lymphoma-2 (Bcl-2) were detected by Western blotting, and the ratio of Bax to Bcl-2 was calculated. Apoptotic rate was detected by flow cytometry. The mRNA expressions of tumor necrosis factor alpha (TNF-α) and interleukin 1beta (IL-1β) in cells were detected by real-time fluorescent quantitative reverse transcription polymerase chain reaction technique. Content of TNF-α and IL-1β in cell culture supernatant was detected by enzyme-linked immunosorbent assay. Data were statistically analyzed with t test, one-way analysis of variance, and least significant difference test. Results:(1) At 48 hours after transfection, the protein expression of 14-3-3σin cells of PCMV6-14-3-3σgroup (1.05±0.03) was significantly higher than that in control group (0.78±0.04, t=5.41, P<0.01). (2) Compared with those in control group, the ratio of Bax to Bcl-2, apoptotic rate, mRNA expressions of TNF-α and IL-1β, and content of TNF-α and IL-1β in cell supernatant in PCMV6-14-3-3σgroup showed no significant difference ( P>0.05); the above-mentioned indexes of cells in LPS group were significantly higher or increased ( P<0.01). Compared with those in LPS group, the above-mentioned indexes of cells in PCMV6-14-3-3σ+ LPS group were significantly lower or decreased ( P<0.01). Conclusions:14-3-3σis a key factor in regulating apoptosis. It can alleviate the LPS-induced inflammatory responses by regulating the ratio of apoptotic regulators Bax to Bcl-2 and inhibiting apoptosis of human pulmonary epithelial cells.

Objective To fabricate DVDMS-Mn-LPs and evaluate its potential on fluorescence mo-lecular imaging, MRI and sonodynamic therapy ( SDT) . Methods DVDMS was used to chelate with Mn2+, and then encapsulated into nanoliposomes by a typical thin-film rehydration method to fabricate DVDMS-Mn-LPs. The particle morphology, average diameter, zeta potential, encapsulation efficiencies and Mn2+content were determined. Fluorescence molecular imaging was performed on different concentrations of DVDMS-Mn-LPs (0, 40, 80, 120, 160 μg/ml) with the small animal fluorescence imaging system. T1WI was per-formed with 3.0 T MR. The SDT effect of DVDMS-Mn-LPs was verified by CCK8 assay among the following groups:control group, ultrasound group ( experimental group 1, EG1) , DVDMS-Mn-LPs group ( EG2) and DVDMS-Mn-LPs + ultrasound group ( EG3) . One-way analysis of variance and the least significant differ-ence t test were used to analyze the data. Results DVDMS-Mn-LPs exhibited a well-defined spherical mor-phology and homogeneous distribution. The encapsulation efficiency was (65.56±1.47)%. ICP-AES meas-urement revealed that the chelation of Mn2+ with DVDMS occurred at a molar ratio of 1. 6:1. The fluores-cence intensities progressively increased with the elevated concentrations of DVDMS-Mn-LPs. The r1 value for DVDMS-Mn-LPs was 23.74 mmol·L-1·s-1. The cell viabilities of EG3 was (54.82±8.55)%, which were significantly lower than those of EG2 and EG1 ((86.54±2.67)% and (83.76±6.48)%;F=6011, t values: -8.35, -9.15, all P<0.001). Conclusion DVDMS-Mn-LPs is successfully fabricated and has good potential on fluorescence molecular imaging, MRI and SDT, which provides a promising imaging-guided modality for glioma treatment in vivo.

Very long chain polyunsaturated fatty acids (VLC-PUFAs) are unique fatty acids in tissues of mammals such as retina and testis, and the key enzyme of its biosynthesis is very long chain fatty acid elongase 4 (Elovl4). Development of an animal model of tissue-specific knockout of Elovl4 gene is conducive to the in-depth study of the biological function of VLC-PUFAs. Therefore, we constructed Stra8-Cre mice and Elovl4 floxed mice based on Cre/loxP system, and obtained the (Elovl4[flox/+], Stra8-Cre) heterozygous knockout mice by hybridization. Subsequently, female mice were selected to cross with male mice with homozygous Elovl4[flox/flox] to gain homozygous mice (Elovl4[flox/flox], Stra8-Cre) through genotype identification and screening. RT-PCR, qRT-PCR, Western blotting, immunohistochemistry and immunofluorescence techniques were used to detect the knock-out efficiency of Elovl4 in testis. The expression of Elovl4 in testis of both heterozygous and homozygous knockout mice were significantly down-regulated at mRNA and protein levels, but were not affected in other tissues. In summary, we constructed a mouse model with specific knockout of Elovl4 gene in testis, which provides a reliable animal model for studying the effect of VLC-PUFAs on the reproductive function of male mice and the underpinning molecular mechanisms.
Animals ; Disease Models, Animal ; Eye Proteins/metabolism* ; Female ; Gene Knockout Techniques ; Integrases ; Male ; Mammals/metabolism* ; Membrane Proteins/metabolism* ; Mice ; Mice, Knockout ; Testis/metabolism*

Patients with severe trauma require an extremely timely treatment and transfusion plays an irreplaceable role in the emergency treatment of such patients. An increasing number of evidence-based medicinal evidences and clinical practices suggest that patients with severe traumatic bleeding benefit from early transfusion of low-titer group O whole blood or hemostatic resuscitation with red blood cells, plasma and platelet of a balanced ratio. However, the current domestic mode of blood supply cannot fully meet the requirements of timely and effective blood transfusion for emergency treatment of patients with severe trauma in clinical practice. In order to solve the key problems in blood supply and blood transfusion strategies for emergency treatment of severe trauma, Branch of Clinical Transfusion Medicine of Chinese Medical Association, Group for Trauma Emergency Care and Multiple Injuries of Trauma Branch of Chinese Medical Association, Young Scholar Group of Disaster Medicine Branch of Chinese Medical Association organized domestic experts of blood transfusion medicine and trauma treatment to jointly formulate Chinese expert consensus on blood support mode and blood transfusion strategies for emergency treatment of severe trauma patients ( version 2024). Based on the evidence-based medical evidence and Delphi method of expert consultation and voting, 10 recommendations were put forward from two aspects of blood support mode and transfusion strategies, aiming to provide a reference for transfusion resuscitation in the emergency treatment of severe trauma and further improve the success rate of treatment of patients with severe trauma.