Child ; Humans ; Tuberculosis ; mortality

Objective:To evaluate bacteriostasis effect of domestic-made water-soluble propolis on prevotella intermedia(Pi),fu-sobacterium nucleatum(Fn)and mixture bacteria of Pi and Fn in vitro.Methods:With agar dilution,minimal bactericidal con-centrations(MBC)of propolis for Fn,Pi and mixture bacteria were determined.Results:MBC of propolis for Fn,Pi or mixture bacteria were 0.025%,0.025%,0.05% respectively.Conclusion:Propolis shows the favourable antibacterial activity against the main anaerobic bacterium in infectious root canal which it has a value of application for disinfecting root canal.

Objective:To investigate the pluripotency of human embryonic stem cells in vitro.Meth-ods:Human embryonic stem cells were cultured in suspension without feeder layers and bFGF in medi-um.mRNAs of different types of cells were analyzed by RT-PCR.Results:When Human embryonicstem cells were cultured in suspension without feeder layers and bFGF in medium,they formed floatingaggregates termed embryoid bodies(EBs),in which many precursors and functional cells were detectedby RT-PCR,such as neural and islet precursors,neurons,insulin-secreting cells,glucagon-secretingcells and liver cells et al.Conclusion:Human embryonic stem cells are able to form embryoid bodiesnaturally in vitro which express specific markers of many types of precursors and mature functional cells.

Objective To explore the diagnosis value by acoustic halo sign in hepatocellular carcinoma.Methods Ultrasonography was performed on 88 echogenic areas in livers,to view the acoustic halo sign around them.And to prepare with the clinicopathological characteristics.Results Around all the echogenic areas,there were 51 with the acoustic halo sign,50 echogenic areas were hepatocellular carcinoma.There were 37 without the acoustic halo sign,18 echogenic areas were hepatocellular carcinoma.Conclusion The acoustic halo sign has high value in diagnosis of hepatocellular carcinoma.

Objective:To establish the identification method of Jianpigao Tablets.Methods: The microscopic identification and TLC were used. Results: 8 herbs in Jianpigao Tablets can be identified by microscopic identified. Radix Codonopsis, Rhizoma atractylodis macrocephalea and Radix glycyrrhizae can be identified respectively by TLC.Conclusions: The method is accurate and simple, and can be used for quality control of Jianpigao Tablets.

Objective :To establish the consensus sequence of PreS/S and Enhll/CP/PreC in Chongqing,China. Methods :The genes of PreS/S and Enhll/CP/PreC of HBV in 15 AsC were sequenced. The genotype and serotype of HBV were determined. The HBV strain prevailing in Chongqing was found and its consensus sequence was established. Results: Nine strains were genotype B/serolype adw2, 3 were genotype B/serotype aywl and 3 were genotype B/serotype adrq+ . There were 6 and 1 site differences between the consensus sequences of PreS/S and Enhll/CP/PreC of HBV with genotype B/serotype adw2 and that in Southeast China. Conclu-sions:The consensus sequence of PreS/S and Enhll/CP/PreC of hepatitis B virus prevailing in Chongqing was established.

Objective:To clarify the possibility of higher sensitivity to ototoxicity of aminoglycosides in neonatal animal.Methods:The difference in ototoxicity was studied by scanning electron-microscopy and microscopy between neonatal and adult groups of 36 guinea pigs administrated with therapeutic doses of amikacin.Pharmacokinetics was investigated in 10 guinea pigs in each group by TDx system.Results:(1)The pharmacokinetics were compatible with the model of two compartment in all age groups.There was no significant difference in the peak level and peak time.Compared with adult group, the plasma clearance was lower(0.0044?0.0011 and 0.0088?0.0014 L/min. kg, P

Objective:To explore the angiogenic ability of vascular endothelial cells in solid tumors.Methods:Immunocytochemistry,reverse transcription polymerase chain reaction (PT-PCR),and two-well co-culture system were used to detect the expressions of matrix metalloproteinase 9(MMP-9),transforming growth factor-?1(TGF-?1),tenascin(TN)and bcl-2,telomerase activity,and their ability of both migration and tube formation of ECV304-SKOV3 cells respectively,which ,as the cell model,had been established in our laboratory before.Then their abilities of protein synthesis and adhesion were analysed.Results:The ability to synthesize proteins and telomerase activity was enhanced(P

Objective:To explore the synergism of vascular endothelial cells and fibroblasts derived from tumors in the tumor angiogenesis after the establishment of the cell models.Methods:Reverse transcription polymerase chain reaction,two-well co-culture system were used to detect the tolomerase activity,and their abilities of migration and angiogenesis of ECV304-SKOV3 cells and the potential of L_(929)-H_(22) to promote angiogenesis and invasion of MDA-MB-231 cells,respectively,which were established as the cell models for vascular endothelial cells and fibroblasts derived from tumors in our laboratory before.Results:Tolomerase activity level of ECV304-SKOV3 cells was higher than that of ECV304 cells (0.778?0.011 vs 0.692?0.014,P

Objective: To invistigate the characteristics of mutations in EnhII/CP/PreC of HBV in AsC children infected through mother-to-infant transmission and their AsC mothers with different degrees of viremia.Methods: There were 15 pairs of children and mothers.They were divided into three groups based on the level of viremia: groupⅠ(both children and mothers presented high viremia),groupⅡ(children presented high and mothers presented low viremia) and groupⅢ(children presented low and mothers were high viremia).There were 5 pairs in each group with 4/5 of HBV being B/adw2 and 1/5 of HBV being C/adrq+ in each group.EnhII/CP/PreC was amplified and cloned into pGEM-T vector.Tow clones were selected to sequence each patient.Results: Substitution was the main model of mutations.There were no significant differences in the mutation numbers in EnhII/CP/PreC among high viremia groups or between two low viremia groups.There were significant differences between each high viremia group and each low viremia group.In patients with high viremia,there were 6 mutational positions in 32 clones in 16/20 patients with B/adw2,and there were 4 mutational positions in 8 clones in 4/20 patients with C/adrq+.In patients with low viremia(mothers of group Ⅱ and children of group Ⅲ),there were 26 mutational positions totally.Conclusion: The mutations in EnhII/CP/PreC are related to the degree of viremia in AsC children infected through mother-to-infant transmission and their AsC mothers,and no associated with age.