Child ; Humans ; Tuberculosis ; mortality

Hereditary Autoinflammatory Diseases ; Humans

Objective To provide basic datas for enriching the scientific and reasonable security strategy of blood transfusion in Chongqing ,even for the national use in the future ,we studied the infection status of human parvovirus B19(B19V) among unpaid blood donors and evaluated the prevalence of B19V in Chongqing .Methods B19 IgM and B19 IgG antibody from blood donors were detected by the method of enzyme linked immunosorbent assay .Results Among 1 104 blood samples of blood donors ,the positive rate of B19 IgM was 5 .07% ,and the positive rate of B19 IgG was 36 .68% ,there was a significant difference between them(P<0 .05) .The positive rate of B19 IgM from females was higher than males′(P<0 .05) ,but there was no statistical difference for the positive rate of B19 IgG between different gender(P> 0 .05) .Comparing with various age groups ,There was still no significant difference of the positive rate of B19 IgM among them(P>0 .05) ,but the positive rates of B19 IgG increased with age increasing (P<0 .05) .The different blood types or collection seasons ,and the statistical difference were not established in these two condi‐tions (P>0 .05) .And there was no significant difference for positive rate of B19 IgM between positive samples and negative sam‐ples of HBV ,HCV ,HIV ,the results of B19 IgG also were such(P> 0 .05) .Conclusion Among blood donors in Chongqing ,al‐though there was no screening for B19V ,we still found infections by B19V .So this may illustrated that the risk of blood transfusion by B19V was still existed .

Diagnostic criteria for coeliac disease(CD) from the European Society for Paediatric Gastroenterology,Hepatology,and Nutrition were published in 1990.Since then,the autoantigen in CD,tissue transglutaminase had been identified;CD-specific antibody tests had improved.The perception of CD had changed greatly.A panel of 17 experts defined CD and developed new diagnostic criteria based on the Delphi process in 2012.The 2004 National Institutes of Health/Agency for Healthcare Research and Quality report and a systematic literature search on antibody tests for CD in paediatric patients covering the years 2004 to 2009 was the basis for the evidence-based recommendations on CDspecific antibody testing.In group of children with symptoms suggestive of CD,the diagnosis of CD is based on symptoms,positive serology,and histology that is consistent with CD.If immunoglobulin A anti-tissue transglutaminase type 2 antibody titers are high (＞ 10 times the upper limit of normal),then the option is to diagnose CD without duodenal biopsies by applying a strict protocol with further laboratory tests.In group of asymptomatic children at increased risk for CD,the diagnosis of CD is based on positive serology and histology.Human leukocyte antigen-DQ2 and human leukocyte antigen-DQ8 testing is valuable because CD is unlikely if both haplotypes are negative.The aim of the new guidelines was to achieve a high diagnostic accuracy and to reduce the burden for patients and their families.

Objective:To evaluate the safety and feasibility of root canal therapy in leukemia patients before bone marrow transplantation.Methods:15 root canal therapy of teeth with chronic periapical diseases were performed in 15 patients with leukemia before bone marrow transplantation.Diameters of the shaded area of periapex were all less than 0.5 cm.The root canal therapy was done at appropriate time,restricted hemateikon(neutrophil counts range from 3.0?109/L ~10?109/L,platelet counts more than 50?109/L),all the teeth were done with root canal therapy according to the principle of root canal therapy.All the patients were monitored by return visit after transplantation.Results:It did not happen any odontogenous complication with 15 patients during the bone marrow transplantation period.The periapical diseases of 15 teeth have been well controlled after root canal therapy.Conclusion:Teeth with chronic periapical diseases of patients with leukemia before bone marrow transplantation can be preserved after adequate root canal therapy and the root canal therapy is safe.

Objective:To evaluate bacteriostasis effect of domestic-made water-soluble propolis on prevotella intermedia(Pi),fu-sobacterium nucleatum(Fn)and mixture bacteria of Pi and Fn in vitro.Methods:With agar dilution,minimal bactericidal con-centrations(MBC)of propolis for Fn,Pi and mixture bacteria were determined.Results:MBC of propolis for Fn,Pi or mixture bacteria were 0.025%,0.025%,0.05% respectively.Conclusion:Propolis shows the favourable antibacterial activity against the main anaerobic bacterium in infectious root canal which it has a value of application for disinfecting root canal.

Objective:To establish the identification method of Jianpigao Tablets.Methods: The microscopic identification and TLC were used. Results: 8 herbs in Jianpigao Tablets can be identified by microscopic identified. Radix Codonopsis, Rhizoma atractylodis macrocephalea and Radix glycyrrhizae can be identified respectively by TLC.Conclusions: The method is accurate and simple, and can be used for quality control of Jianpigao Tablets.

Objective :To establish the consensus sequence of PreS/S and Enhll/CP/PreC in Chongqing,China. Methods :The genes of PreS/S and Enhll/CP/PreC of HBV in 15 AsC were sequenced. The genotype and serotype of HBV were determined. The HBV strain prevailing in Chongqing was found and its consensus sequence was established. Results: Nine strains were genotype B/serolype adw2, 3 were genotype B/serotype aywl and 3 were genotype B/serotype adrq+ . There were 6 and 1 site differences between the consensus sequences of PreS/S and Enhll/CP/PreC of HBV with genotype B/serotype adw2 and that in Southeast China. Conclu-sions:The consensus sequence of PreS/S and Enhll/CP/PreC of hepatitis B virus prevailing in Chongqing was established.

Objective:To explore the angiogenic ability of vascular endothelial cells in solid tumors.Methods:Immunocytochemistry,reverse transcription polymerase chain reaction (PT-PCR),and two-well co-culture system were used to detect the expressions of matrix metalloproteinase 9(MMP-9),transforming growth factor-?1(TGF-?1),tenascin(TN)and bcl-2,telomerase activity,and their ability of both migration and tube formation of ECV304-SKOV3 cells respectively,which ,as the cell model,had been established in our laboratory before.Then their abilities of protein synthesis and adhesion were analysed.Results:The ability to synthesize proteins and telomerase activity was enhanced(P

Objective:To explore the synergism of vascular endothelial cells and fibroblasts derived from tumors in the tumor angiogenesis after the establishment of the cell models.Methods:Reverse transcription polymerase chain reaction,two-well co-culture system were used to detect the tolomerase activity,and their abilities of migration and angiogenesis of ECV304-SKOV3 cells and the potential of L_(929)-H_(22) to promote angiogenesis and invasion of MDA-MB-231 cells,respectively,which were established as the cell models for vascular endothelial cells and fibroblasts derived from tumors in our laboratory before.Results:Tolomerase activity level of ECV304-SKOV3 cells was higher than that of ECV304 cells (0.778?0.011 vs 0.692?0.014,P