1.High Wet Package Rate of Rent Surrgical Instrument:Reasons and Countermeasures
Jun ZHAO ; Jiangling YI ; Honglu WU
Chinese Journal of Nosocomiology 2004;0(10):-
OBJECTIVE To explore the reasons of high wet package rate of the rent surgical instrument and to look for improvement method.METHODS A total of 2872 packages of hospitals in Chongqing city were investigated during the last two years.RESULTS Among 2872 rent surgical instrument packages,1064 packages were found to be damp(37.05%).CONCLUSIONS Unstandardized and too large packaging are the main reasons.After elimination of above two reasons,the damp rate will be decreased.
2.Effects of Aconite Alkaloids on Apoptosis and Cell Cycle of Human Gastric Cancer Cell Line SGC-7901
Yunxia DING ; Xi ZOU ; Jian WU ; Honglu GE ; Shouyou HU
World Science and Technology-Modernization of Traditional Chinese Medicine 2013;(5):915-919
This study was aimed to investigate effect of aconite alkaloids on proliferation and apoptosis of hu-man gastric cancer cell line SGC-7901 . Effects of different concentrations of aconite alkaloids on proliferation of human gastric cancer cell line SGC-7901 were investigated with MTT assay; induced apoptosis and cell cy-cle blocking were detected with flow cytometer ( FCM ) . The results showed that the IC50 of aconite alkaloids on human gastric cancer cell line SGC-7901 was 0 . 2318 ± 0 . 0022 , 0 . 1601 ± 0 . 0227 , 0 . 1031 ± 0 . 0231 mg/ml at 24 h , 48 h and 72 h , respectively , compared to the control group with significant difference ( P <0.01). When the aconite alkaloids concentration was 0.8 mg/ml, it appeared with obvious apoptosis. The apop-tosis rate was ( 59 . 38 ± 5 . 05 )%. The FCM detection showed that compared with control group , the percentage of S-phase cells increased in the treatment group . And a typical sub-diploid peak appeared before G0 / G1 phase . It was concluded that aconite alkaloids can inhibit the proliferation of human gastric cancer cell line SGC-7901 in vitro, induce the apoptosis of cells and make SGC-7901 cells remain in S phase.