Cyclooxygenase-2 (COX-2) is the rate-limiting enzyme in the production of arachidonic acid to PG, and was differently expressed in leukemia, which may be involved in the progression of leukemia. The specific COX-2 inhibitor might become a new target for the therapy of leukemia. In this article, the effect of COX-2 in pathogenesy of leukemia and application of COX-2 inhibitor in leukemia are reviewed.

Objective To study on the correlation between plasma homocysteine (Hcy),folate acid(FA) and vitamin B12(VitB12) levels and vascular dementia (VD).Methods To measure plasma Hcy, FA and vitamin B12 in 96 patients with VD ,in 152 non-dementia cerebrovascular disease ( NDCVD ) and in 80 normal controls re-spectively,and results werw compared between each two groups by using student test .Cognitive status was evaluar by using mini mentai state examination(MMSE).The effect of Hcy,FA and VitB12 on VD and its cognitive function were analyzed.Results The levels of plasma Hcy in VD and NDCVD were higher than those in normal control grop ,and there was significantly difference (t=3.26,3.14,all P<0.01),and the best high one is VD;The levels of plasma FA and VitB12 in VD and NDCVD were lower than those in normal control grop ,and there was significantly difference ( t=3.16,2.98,3.21,2.91,all P<0.01),and the best low one is VD.The levels of plasma Hcy concentrations were in-creased and MMSE scores were decreased with severity of dementia , and there were negative correlation between MMSE scores and plasma Hcy concentrations (r=-0.69,P<0.05).Conclusion The levels of plasma Hcy corre-lated with VD.High-Hcy is one of possible rise factor for VD in senior .Both low-FA and low-VitB12 were indirect fac-tors for VD.

Objective To study the method of cryopreservation, and method of cultivation of hepatocytes of sucking pig. Methods Hepatocytes were isolated from sucking pig by two-step perfusion with collagenase using an extracorporeal perfusion apparatus. They were cryopreserved in liquid nitrogen for 4 months. They were thawed and seeded on gelatin coated bottom of a flask. The cells were covered with another layer of collagen. The exprssion of albumin mRNA, cellular morphology synthesis of urea, and content of oxalylacetylaminotransferase were determined in the collagen cultures. Results Thawed hepatocytes maintained their biological characters well after cryopreservation. The thawed hepatocytes could grow well in collagen gel, sandwich culture. In the 10-day cultures, typical cellular morphological and biological characteristics of cultured hepatocytes could be observed. Conclusion Hepatocytes stored in liquid nitrogen could maintain their activities for a long time. The hepatocytes could be cultivated in collagenous matrix, which provided an environment closely resembling that in nature, and their morphology and functions could be maintained.

Leukemia is a kind of cloning disease from malignant hematopoietic stem cells. Its patho-genesis may have relationship with proliferation out of control,dysdiffreentiation and inhibition of apoptosis about leukemia cells. The study confirms that CD44 is expressed in leukemia cells,promoting the occurrence and development of leukemia through participation in cell adhension,proliferation,migration and invasion behavior. In vitro experiment shows that CD44 targeted therapies can effectively kill leukemia cells,and reduce the toxicity to normal tissue cells. Therefore,aiming at the design of the anticancer drugs targeted for CD44 is expected to be used in clinical treatment of leukemia.

OBJECTIVE:To explore the effects of Haikangling Mixture on immunological function in immunosuppressed mice induced by cyclophosphamide. METHODS: Mice were given Haikangling Mixture intragastrically for 14d, and the level of hemolysin was determined by sheep erythrocyte (SRBC) immunization to exploer the influence of Haikangling on humoral immunity in mice; splenic lymphocytes were measured by MTT to observe the influence on cell immunity; the clearance of macrophage were measured by carbon particle clearance method to explore the influence on non-specificity immunity. RESULTS: The level of hemolysin, the cell reproductive activity, splenic lymphocytes and the phagotrophy of mononuclear macrophage were improved obviously by Haikangling Mixture in immunosuppressed mice induced by cyclophosphamide. CONCLUSION: Haikangling Mixture can enhance the immunological function in immunosuppressed mice induced by cyclophosphamide.

OBJECTIVE:To develop an HPLC method for the determination of L-Carnosine in Polaprezine granules.METHODS:The Chromatographic column was Agilent ODS with detection wavelength of 215 nm and flow rate of 1 mL?min-1.The mobile phase consisted of sodium subsulfite buffer solution(pH=3)-acetonetrile(77:23).The column temperature was set at 30 ℃ and injection size was 10 ?L.RESULTS:The good linearity was obtained in the range of 0.1559～3.1584 ?g for L-Carnosine(r=1.0000).The average recovery was 99.65%(RSD=0.078%).CONCLUSION:The method is simple,accurate and sensitive,and it is suitable for content determination of L-Carnosine in Polaprezine granules.

Objective To evaluate the clinical efficacy of percutaneous vertebroplasty (PVP) with flexible bone cement injector through unilateral puncture. Method Unilateral puncture line PVP treatment for thoracolumbar vertebral compression fracture was conducted in 78 patients, which was divided into observation group and control group according to the use of different equipment. The flexible bone cement injector was used in 36 cases of observation group, which injected the bone cement into three places at the vertebral body. The ordinary straight bone cement injector was applied in the 42 cases of the control group. The operation time, the time of radiation exposure, and the amount of bone cement injection were recorded, and the leakage of bone cement was observed. The visual analogue scale (VAS), relative vertebral height and Cobb angle were recorded at 1 week, 1 and 2 years follow-up. The time of operation, the time of radiation exposure, the amount of bone cement injection, the incidence of bone cement leakage, VAS, relative height of vertebral body and Cobb angle were compared between the two groups using independent samples t test orχ2 test. Result All patients in the two groups were successfully treated by surgery. There were no significant differences between the observation group and the control group in the operation time and the time of radiation exposure and the incidence of bone cement leakage (P>0.05). The injection amounts of bone cement in the observation group and the control group were (5.6 ± 0.7) and (3.2 ± 0.6) ml, with statistically significant difference (P<0.05). There were no significant differences in VAS score, relative vertebral height and Cobb angle between the two groups at 1 week after operation (P>0.05). At 1 year and 2 years after operation, the differences of the above indexes between the two groups were significantly different (P<0.05), with the observation group being superior to the control group. Conclusions Application of flexible bone cement injector for PVP operation is safe and feasible. Compared with the traditional straight bone cement injector, the use of flexible bone cement injector will not increase the operation time, radiation exposure time and the incidence of bone cement leakage but will improve the long-term effect.

The CNKI database was searched using the keywords premature ovarian failure and acupuncture. Ninety-eight related articles published from 2006 to 2015 were retrieved and 17 with randomized controlled trials were sifted out. The recent 10 years' research situation of the mechanism of acupuncture treatment for premature ovarian failure was systematically summarized through literature review and collation. A retrospective summary was made from five aspects: improvement in ovarian endocrine function, acceleration of ovulation, effect on immunologic system, improvement in ovarian reserve function and regulation of ovarian microenvironment. It is put forward on this basis that future studies on the mechanism of acupuncture treatment for premature ovarian failure can be further into higher gene and protein levels; and no matter what kind of acupuncture manipulation is used, arrival of qi is necessary; only arrival of qi can produce an effect.

Objective To explore the influence of monosialoglycans ganglioside(GM1) on dynamic changes of plasma BDNF and NSE in the treatment of neonatal hypoxic-ischemic encephalopathy(HIE).Methods According to the digital table,82 children with HIE were randomly divided into study group and control group, 41 cases in each group.The study group was given GM1 treatment,the control group was given routine treatment.The BDNF and NSE levels after birth 1,7,14 d of the two groups were detected.Results The BDNF levels in the study group at 7,14 days were higher than those in the control group[(1 823.3±286.2)pg/mL vs (1 368.3±229.2)pg/mL,t=3.07,P<0.01;(958.3±89.3)pg/mL vs (863.2±88.6)pg/mL,t=2.03,P<0.05].The NSE levels of moderate and severe children in the study group at 7 days were lower than those in the control group[(9.55±3.32)ng/L vs (14.31±2.52)ng/L,(20.22±3.63)ng/L vs (32.05±5.73)ng/L,t=5.32,4.69,all P<0.05].The incidence rate of complication in the study group was 4.88%,which was lower than 19.51% in the control group,the difference was statistically significant(x2=4.10,P<0.05).Conclusion GM1 in the treatment of HIE can achieve significant effect,and the plasma BDNF and NSE levels increase and decrease rapidly.

Enterovirus A71 (EV-A71) causes hand, foot, and mouth disease (HFMD) and various neurological complications, including aseptic meningitis and neurogenic pulmonary edema in young children. HFMD caused by EV-A71 have broken out several times in the Asia-Pacific region since 2007. And it has been a serious threat to public health. There is no effective vaccine or antiviral drug. The pathogenesis of EV-A71 infection is unknown, and EV-A71 3C protein plays an irreplaceable role in replication and anti - innate immunity. Further research on EV-A71 3C protein is conducive to understand the pathogenesis of EV-A71 infection and antiviral drug.
Animals ; Antiviral Agents ; pharmacology ; Enterovirus ; drug effects ; immunology ; metabolism ; physiology ; Humans ; Immunity, Innate ; Viral Proteins ; chemistry ; genetics ; metabolism ; Virus Replication ; drug effects